{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:22:25Z","timestamp":1772166145548,"version":"3.50.1"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T00:00:00Z","timestamp":1628208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T00:00:00Z","timestamp":1628208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Clin Microbiol Antimicrob"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The resistance of\n                      <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                      to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in\n                      <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                      (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different \u03b2-lactams and fluoroquinolones.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case presentation<\/jats:title>\n                    <jats:p>\n                      A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (\n                      <jats:italic>penA<\/jats:italic>\n                      ,\n                      <jats:italic>ponA<\/jats:italic>\n                      ,\n                      <jats:italic>porinB<\/jats:italic>\n                      ,\n                      <jats:italic>mtr<\/jats:italic>\n                      ,\n                      <jats:italic>gyrA<\/jats:italic>\n                      ,\n                      <jats:italic>parC<\/jats:italic>\n                      , 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the\n                      <jats:italic>porinB<\/jats:italic>\n                      gene were observed, and data on this gene are limited. Information on the identified allele type of the\n                      <jats:italic>penA<\/jats:italic>\n                      gene is very limited as well. Three different mutations of\n                      <jats:italic>parC<\/jats:italic>\n                      and\n                      <jats:italic>gyrA<\/jats:italic>\n                      that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12941-021-00456-5","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T11:03:31Z","timestamp":1628247811000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular characterization of a ceftriaxone-resistant Neisseria gonorrhoeae strain found in Switzerland: a case report"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3029-4428","authenticated-orcid":false,"given":"Konrad","family":"Egli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Roditscheff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Fl\u00fcckiger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Risch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenz","family":"Risch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Bodmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,6]]},"reference":[{"key":"456_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2334-13-603","volume":"13","author":"H Kovari","year":"2013","unstructured":"Kovari H, de Melo Oliveira MDG, Hauser P, L\u00e4uchli S, Meyer J, Weber R, et al. Decreased susceptibility of Neisseria gonorrhoeae isolates from Switzerland to cefixime and ceftriaxone: antimicrobial susceptibility data from 1990 and 2000 to 2012. BMC Infect Dis. 2013;13:1\u20135.","journal-title":"BMC Infect Dis"},{"key":"456_CR2","unstructured":"European Committee on Antimicrobial Susceptibility Testing. Testing breakpoint tables for interpretation of MICs and zone diameters Version 9. 2019. p. 0\u201377. www.eucast.org"},{"issue":"10","key":"456_CR3","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.10.1900147","volume":"24","author":"DW Eyre","year":"2019","unstructured":"Eyre DW, Town K, Street T, Barker L, Sanderson N, Cole MJ, et al. Detection in the United Kingdom of the Neisseria gonorrhoeae FC428 clone, with ceftriaxone resistance and intermediate resistance to azithromycin, october to december 2018. 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