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This study aimed to evaluate the antibacterial and antibiofilm-formation effects of zinc oxide nanoparticles (ZnO-NPs) against <jats:italic>staphylococcus aureus<\/jats:italic> (<jats:italic>S. aureus)<\/jats:italic> isolates.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A total of 116 <jats:italic>S. aureus<\/jats:italic> isolates were recovered from 250 burn wound samples. The antimicrobial\/antibiofilm effects of ZnO-NPs against methicillin, vancomycin and linezolid resistant <jats:italic>S. aureus<\/jats:italic> (MRSA, VRSA and LRSA) isolates were examined using phenotypic and genotypic methods. The minimum inhibitory concentration (MIC) of ZnO-NPs was determined by microdilution method. The effects of sub-MIC concentrations of ZnO-NPs on biofilm formation and drug resistance in <jats:italic>S. aureus<\/jats:italic> were determined by the microtiter plate method. The change in the expression levels of the biofilm encoding genes and resistance genes in <jats:italic>S. aureus<\/jats:italic> isolates after treatment with ZnO-NPs was assessed by real time reverse transcriptase PCR (rt-PCR).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>MICs of ZnO-NPs in <jats:italic>S. aureus<\/jats:italic> isolates were (128\u20132048\u00a0\u00b5g\/ml). The sub-MIC of ZnO-NPs significantly reduced biofilm formation rate (the highest inhibition rate was 76.47% at 1024 \u00a0\u00b5g\/ml) and the expression levels of biofilm genes (<jats:italic>ica A, ica D<\/jats:italic> and <jats:italic>fnb A)<\/jats:italic> with P\u2009&lt;\u20090.001. Moreover, Sub-MIC of ZnO-NPs significantly reduced the rates of MRSA from 81.9 (95 isolates) to 13.30% (15 isolates), VRSA from 33.60 (39 isolates) to 0% and LARSA from 29.30 (34) to 0% as well as the expression levels of resistance genes (<jats:italic>mec A, van A<\/jats:italic> and <jats:italic>cfr<\/jats:italic>) with P value &lt;\u20090.001.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>ZnO-NPs can be used as antibiofilm and potent antimicrobial against MRSA, VRSA and LRSA isolates.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12941-021-00459-2","type":"journal-article","created":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T10:02:56Z","timestamp":1629540176000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates"],"prefix":"10.1186","volume":"20","author":[{"given":"Wedad M.","family":"Abdelraheem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha M. 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