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The isolated VNS-CoNS were evaluated with Matrix-assisted Laser Desorption\/ionization Time of Flight Mass Spectrometry (MALDI ToF MS) for species characterization and pan-antimicrobial resistance pattern.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Out of 256 clinical samples collected including pus, abscess, ear swabs, eye swabs, and aspirates, 91 CoNS isolates were biotyped and further characterized with MALDI-TOF MS. Staphylococci marker genes, Vancomycin susceptibility, and biofilm assays were performed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Of 91 CoNS isolates,\u00a0<jats:italic>S.cohnii<\/jats:italic>\u00a0(2.3%),\u00a0<jats:italic>S.condimentii<\/jats:italic>\u00a0(3.4%),\u00a0<jats:italic>S. saprophyticus<\/jats:italic>\u00a0(6.7%),\u00a0<jats:italic>and S.scuri<\/jats:italic>\u00a0(21.1%) were characterized with MALDI-TOF with significant detection rate (99.4%; CI 95, 0.775\u20130.997, positive predictive values, 90.2%) compared to lower biotyping detection rate (p\u2009=\u20090.001). Hemolytic VNS-CoNS lacked\u00a0<jats:italic>nuc, pvl<\/jats:italic>\u00a0and\u00a0<jats:italic>spa<\/jats:italic>\u00a0genes from wound, ear, and aspirates of more 0.83 MARI clustered into a separate phylo-diverse group and were widely distributed in urban and peri-urban locations. MALDI TOF\u2013MS yielded a high discriminatory potential of AUC-ROC score of 0.963 with true-positivity prediction. VNS-CoNS of MIC\u2009\u2265\u200916\u00a0\u00b5g\/mL were observed among all the ages with significant resistance at 25th and 75th quartiles. More than 10.5% of CoNS expressed multi-antibiotic resistance with more than 8\u00a0\u00b5g\/mL vancomycin cut-off values (p\u2009&lt;\u20090.05).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Antibiotic resistant CoNS should be considered significant pathogens rather than contaminant. Biofilm producing VNS-<jats:italic>S. sciuri<\/jats:italic>\u00a0and\u00a0<jats:italic>S. condimentii<\/jats:italic>\u00a0are potential strains with high pathological tropism for skin, soft tissues and wound infections, and these strains require urgent surveillance in peri-urban and rural communities.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12941-022-00516-4","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T15:09:11Z","timestamp":1656688151000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections"],"prefix":"10.1186","volume":"21","author":[{"given":"Paul A.","family":"Akinduti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yemisi Dorcas","family":"Obafemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet","family":"Ugboko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maged","family":"El-Ashker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olayemi","family":"Akinnola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chioma Jane","family":"Agunsoye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abiola","family":"Oladotun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno S. 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