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Demographic information, microbiology culture results and laboratory findings, including white blood counts (WBC), C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), and erythrocyte sedimentation rate (ESR) were collected and analyzed. MRSA was confirmed by antimicrobial susceptibility testing. Other MRSA-caused infections were randomly selected for comparison. Twenty-three virulence and antimicrobial resistance (AMR) genes were screened for MRSA strains. Multilocus sequence typing (MLST) and Staphylococcal protein A (spa) typing were performed using PCR amplification and sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the identified pathogens in BJI, MRSA accounted for 21.0% (47\/224). Patients with BJI had high levels of initial CRP, white blood cell count (WBC) and IL-6. ST59 (43.9%) and t437 (37.6%) were the main MRSA subtypes isolated from the children. The major genotypes in BJI were ST59-t437 (29.8%) and ST22-t309 (14.9%), with high carriage of hemolysins including\n                      <jats:italic>hla<\/jats:italic>\n                      (94.4\u2013100%),\n                      <jats:italic>hlb<\/jats:italic>\n                      (66.2\u201393.3%), and\n                      <jats:italic>hld<\/jats:italic>\n                      (100%). Notably, Panton\u2013Valentine leukocidin (\n                      <jats:italic>pvl<\/jats:italic>\n                      ) had a high prevalence (53.3%) in ST22-t309-MRSA. Other virulence genes including\n                      <jats:italic>tst<\/jats:italic>\n                      ,\n                      <jats:italic>seg<\/jats:italic>\n                      and\n                      <jats:italic>sei<\/jats:italic>\n                      were more commonly detected in ST22-t309-MRSA (40.0\u201346.7%) than in ST59-t437-MRSA (4.2\u20139.9%). High-carriage AMR genes in MRSA included\n                      <jats:italic>aph(3\u02b9)\/III<\/jats:italic>\n                      (66.7\u201380%)\n                      <jats:italic>, ermB<\/jats:italic>\n                      (57.5\u201373.3%) and\n                      <jats:italic>ermC<\/jats:italic>\n                      (66.7\u201378.9%). MRSA presented high-resistance to erythromycin (52.0\u2013100%) and clindamycin (48.0\u201392.5%), different genotypes displayed variation in their susceptibilities to antibiotics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The major MRSA genotype in BJI was ST59-t437, followed by ST22-t309, with a higher prevalence of the\n                      <jats:italic>pvl<\/jats:italic>\n                      gene. Continuous surveillance of\n                      <jats:italic>pvl<\/jats:italic>\n                      -positive ST22-t309-MRSA in pediatric BJI infections is thus required.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12941-023-00654-3","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T11:02:58Z","timestamp":1700650978000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus in bone and joint infection among children"],"prefix":"10.1186","volume":"22","author":[{"given":"Pan","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxier","family":"Nijiati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"654_CR1","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1097\/INF.0000000000001635","volume":"36","author":"J Saavedra-Lozano","year":"2017","unstructured":"Saavedra-Lozano J, Falup-Pecurariu O, Faust SN, Girschick H, Hartwig N, Kaplan S, et al. 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