{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T16:05:59Z","timestamp":1794413159470,"version":"4.9.1"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Special Foundation for National Science and Technology Basic Research Program of China","award":["2019FY101200"],"award-info":[{"award-number":["2019FY101200"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Clin Microbiol Antimicrob"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Elizabethkingia<\/jats:italic> is emerging as an opportunistic pathogen in humans. The aim of this study was to investigate the clinical epidemiology, antimicrobial susceptibility, virulence factors, and genome features of <jats:italic>Elizabethkingia<\/jats:italic> spp.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Clinical data from 71 patients who were diagnosed with <jats:italic>Elizabethkingia<\/jats:italic>-induced pneumonia and bacteremia between August 2019 and September 2021 were analyzed. Whole-genome sequencing was performed on seven isolates, and the results were compared with a dataset of 83 available <jats:italic>Elizabethkingia<\/jats:italic> genomes. Genomic features, Kyoto Encyclopedia of Genes and Genomes (KEGG) results and clusters of orthologous groups (COGs) were analyzed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The mean age of the patients was 56.9\u2009\u00b1\u200920.7 years, and the in-hospital mortality rate was 29.6% (21\/71). <jats:italic>Elizabethkingia<\/jats:italic> strains were obtained mainly from intensive care units (36.6%, 26\/71) and emergency departments (32.4%, 23\/71). The majority of the strains were isolated from respiratory tract specimens (85.9%, 61\/71). All patients had a history of broad-spectrum antimicrobial exposure. Hospitalization for invasive mechanical ventilation or catheter insertion was found to be a risk factor for infection. The isolates displayed a high rate of resistance to cephalosporins and carbapenems, but all were susceptible to minocycline and colistin. Genomic analysis identified five \u03b2-lactamase genes (<jats:italic>bla<\/jats:italic><jats:sub>GOB<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>BlaB<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CME<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>OXA<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>) responsible for \u03b2-lactam resistance and virulence genes involved in stress adaptation (<jats:italic>ureB\/G, katA\/B<\/jats:italic>, and <jats:italic>clpP<\/jats:italic>), adherence (<jats:italic>groEL, tufA<\/jats:italic>, and <jats:italic>htpB<\/jats:italic>) and immune modulation (<jats:italic>gmd, tviB, cps4J, wbtIL, cap8E\/D\/G<\/jats:italic>, and <jats:italic>rfbC<\/jats:italic>). Functional analysis of the COGs revealed that \u201cmetabolism\u201d constituted the largest category within the core genome, while \u201cinformation storage and processing\u201d was predominant in both the accessory and unique genomes. The unique genes in our 7 strains were mostly enriched in KEGG pathways related to microRNAs in cancer, drug resistance (\u03b2-lactam and vancomycin), ABC transporters, biological metabolism and biosynthesis, and nucleotide excision repair mechanisms.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The <jats:italic>Elizabethkingia<\/jats:italic> genus exhibits multidrug resistance and carries carbapenemase genes. This study presents a comparative genomic analysis of <jats:italic>Elizabethkingia<\/jats:italic>, providing knowledge that facilitates a better understanding of this microorganism.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12941-024-00691-6","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T17:01:44Z","timestamp":1712768504000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Clinical manifestations, antimicrobial resistance and genomic feature analysis of multidrug-resistant Elizabethkingia strains"],"prefix":"10.1186","volume":"23","author":[{"given":"Chongyang","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanfeng","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"issue":"Pt 3","key":"691_CR1","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1099\/ijs.0.63541-0","volume":"55","author":"KK Kim","year":"2005","unstructured":"Kim KK, Kim MK, Lim JH, Park HY, Lee ST. 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The hospital institutional review board waived the need for informed patient consent because retrospective analysis of the clinical data posed only a minimal risk to the subject and did not involve procedures that normally require written consent outside of the research context.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"32"}}