{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T22:36:36Z","timestamp":1781044596741,"version":"3.54.1"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Clin Microbiol Antimicrob"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Tuberculosis (TB), a major cause of disease and antimicrobial resistance, is spread via aerosols. Aerosols have diagnostic potential and airborne-microbes other than\n                      <jats:italic>Mycobacterium tuberculosis complex<\/jats:italic>\n                      (MTBC) may influence transmission. We evaluated whether PneumoniaCheck (PMC), a commercial aerosol collection device, captures MTBC and the aeromicrobiome of people with TB.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      PMC was done in sputum culture-positive people (\u2265\u200930 forced coughs each,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200916) pre-treatment and PMC air reservoir (bag, corresponding to upper airways) and filter (lower airways) washes underwent Xpert MTB\/RIF Ultra (Ultra) and 16S rRNA gene sequencing (sequencing also done on sputum). In a subset (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096), PMC microbiota (bag, filter) was compared to oral washes and bronchoalveolar lavage fluid (BALF).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>\n                      54% (7\/13) bags and 46% (6\/14) filters were Ultra-positive. Sequencing read counts and microbial diversity did not differ across bags, filters, and sputum. However, microbial composition in bags (\n                      <jats:italic>Sphingobium-<\/jats:italic>\n                      ,\n                      <jats:italic>Corynebacterium-<\/jats:italic>\n                      ,\n                      <jats:italic>Novosphingobium-<\/jats:italic>\n                      enriched) and filters (\n                      <jats:italic>Mycobacterium-<\/jats:italic>\n                      ,\n                      <jats:italic>Sphingobium-<\/jats:italic>\n                      ,\n                      <jats:italic>Corynebacterium-<\/jats:italic>\n                      enriched) each differed vs. sputum. Furthermore, sequencing only detected\n                      <jats:italic>Mycobacterium<\/jats:italic>\n                      in bags and filters but not sputum. In the subset, bag and filter microbial diversity did not differ vs. oral washes or BALF but microbial composition differed. Bags vs. BALF were\n                      <jats:italic>Sphingobium-<\/jats:italic>\n                      enriched and\n                      <jats:italic>Mycobacterium-<\/jats:italic>\n                      ,\n                      <jats:italic>Streptococcus-<\/jats:italic>\n                      , and\n                      <jats:italic>Anaerosinus-<\/jats:italic>\n                      depleted (\n                      <jats:italic>Anaerosinus<\/jats:italic>\n                      also depleted in filters vs. BALF). Compared to BALF, none of the aerosol-enriched taxa were enriched in oral washes or sputum.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>PMC captures aerosols with Ultra-detectable MTBC and MTBC is more detectable in aerosols than sputum by sequencing. The aeromicrobiome is distinct from sputum, oral washes and BALF and contains differentially-enriched lower respiratory tract microbes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12941-024-00735-x","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T10:12:10Z","timestamp":1724407930000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["PneumoniaCheck, a novel aerosol collection device, permits capture of airborne Mycobacterium tuberculosis and characterisation of the cough aeromicrobiome in people with tuberculosis"],"prefix":"10.1186","volume":"23","author":[{"given":"Tinaye L.","family":"Chiyaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina R.","family":"Nyawo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charissa C.","family":"Naidoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suventha","family":"Moodley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose C.","family":"Clemente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanus T.","family":"Malherbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin M.","family":"Warren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David N.","family":"Ku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo N.","family":"Segal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant","family":"Theron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"735_CR1","unstructured":"World Health Organization. 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GT received in-kind donations of PMC devices from MD Innovate, which played no role in the study design or interpretation of results.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Completing interests"}}],"article-number":"74"}}