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Autophagy and circular RNAs (circRNAs) play critical roles in cancer progression and chemoresistance. However, the function and mechanism of circRNA in autophagy regulation of LSCC remain unclear.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>The autophagy-suppressive circRNA <jats:italic>circPARD3<\/jats:italic> was identified via RNA sequencing of 107 LSCC tissues and paired adjacent normal mucosal (ANM) tissues and high-content screening. RT-PCR, Sanger sequencing, qPCR and fluorescence in situ hybridization were performed to detect <jats:italic>circPARD3<\/jats:italic> expression and subcellular localization. Biological functions of <jats:italic>circPARD3<\/jats:italic> were assessed by proliferation, migration, invasion, autophagic flux, and chemoresistance assays using in vitro and in vivo models. The mechanism of <jats:italic>circPARD3<\/jats:italic> was investigated by RNA immunoprecipitation, RNA pulldown, luciferase reporter assays, western blotting and immunohistochemical staining.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Autophagy was inhibited in LSCC, and <jats:italic>circPARD3<\/jats:italic> was upregulated in the LSCC tissues (<jats:italic>n<\/jats:italic>\u00a0=\u2009100, <jats:italic>p<\/jats:italic> &lt; 0.001). High <jats:italic>circPARD3<\/jats:italic> level was associated with advanced T stages (<jats:italic>p<\/jats:italic> &lt; 0.05), N stages (<jats:italic>p<\/jats:italic>\u00a0=\u20090.001), clinical stages (<jats:italic>p<\/jats:italic> &lt; 0.001), poor differentiation degree (<jats:italic>p<\/jats:italic>\u00a0=\u20090.025), and poor prognosis (<jats:italic>p<\/jats:italic>\u00a0=\u20090.002) of LSCC patients (<jats:italic>n<\/jats:italic>\u00a0=\u2009100). Functionally, <jats:italic>circPARD3<\/jats:italic> inhibited autophagy and promoted LSCC cell proliferation, migration, invasion and chemoresistance. We further revealed that activation of the PRKCI-Akt-mTOR pathway through sponging <jats:italic>miR-145-5p<\/jats:italic> was the main mechanism of <jats:italic>circPARD3<\/jats:italic> inhibited autophagy, promoting LSCC progression and chemoresistance.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Our study reveals that the novel autophagy-suppressive <jats:italic>circPARD3<\/jats:italic> promotes LSCC progression and chemoresistance through the PRKCI-Akt-mTOR pathway, providing new insights into circRNA-mediated autophagy regulation and potential biomarker and target for LSCC treatment.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Graphical abstract<\/jats:title>\n\n<\/jats:sec>","DOI":"10.1186\/s12943-020-01279-2","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T05:26:17Z","timestamp":1606195577000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":144,"title":["circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway"],"prefix":"10.1186","volume":"19","author":[{"given":"Wei","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huina","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiwang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuting","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Bo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoya","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1669-3860","authenticated-orcid":false,"given":"Yongyan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"1279_CR1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3322\/caac.21386","volume":"67","author":"CE Steuer","year":"2017","unstructured":"Steuer CE, El-Deiry M, Parks JR, Higgins KA, Saba NF. 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