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The aim of this study was to further elucidate the effect of mutated\n                      <jats:italic>CBS<\/jats:italic>\n                      gene on circulating lipids using a rabbit model harboring a homozygous G307S point mutation in\n                      <jats:italic>CBS.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      CRISPR\/Cas9 system was used to edit the\n                      <jats:italic>CBS<\/jats:italic>\n                      gene in rabbit embryos. The founder rabbits were sequenced, and their plasma homocysteine (Hcy) and lipid profile were analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Six\n                      <jats:italic>CBS<\/jats:italic>\n                      -knockout (\n                      <jats:italic>CBS<\/jats:italic>\n                      -KO) founder lines with biallelic modifications were obtained. Mutation in\n                      <jats:italic>CBS<\/jats:italic>\n                      caused significant growth retardation and high mortality rates within 6\u2009weeks after birth. In addition, the 6-week old\n                      <jats:italic>CBS<\/jats:italic>\n                      -KO rabbits showed higher plasma levels of Hcy, triglycerides (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) compared to the age-matched wild-type (WT) controls. Histological analysis of the mutants showed accumulation of micro-vesicular cytoplasmic lipid droplets in the hepatocytes. However, gastric infusion of vitamin B and betaine complex significantly decreased the plasma levels of TG, TC and LDL-C in the\n                      <jats:italic>CBS<\/jats:italic>\n                      -KO rabbits, and alleviated hepatic steatosis compared to the untreated animals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      A\n                      <jats:italic>CBS<\/jats:italic>\n                      <jats:sup>G307S<\/jats:sup>\n                      rabbit model was generated that exhibited severe dyslipidemia when fed on a normal diet, indicating that G307S mutation in the\n                      <jats:italic>CBS<\/jats:italic>\n                      gene is a causative factor for dyslipidemia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12944-020-01394-5","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T10:03:14Z","timestamp":1602669794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine \u03b2-synthase-deficient rabbit generated using CRISPR\/Cas9"],"prefix":"10.1186","volume":"19","author":[{"given":"Ting","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuguo","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaozheng","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunning","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"issue":"1","key":"1394_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1053\/ejvs.2001.1526","volume":"23","author":"M Hansrani","year":"2002","unstructured":"Hansrani M, Gillespie JI, Stansby G. 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