{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:52:19Z","timestamp":1793109139231,"version":"4.5.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873227"],"award-info":[{"award-number":["81873227"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Lipids Health Dis"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Stroke is the leading cause of death in humans worldwide, and its incidence increases every year. It is well documented that lipids are closely related to stroke. Analyzing the changes in lipid content in the stroke model after absolute quantification and investigating whether changes in lipid content can predict stroke severity provides a basis for the combination of clinical stroke and quantitative lipid indicators.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This paper establishes a rapid, sensitive, and reliable LC\u2012MS\/MS analytical method for the detection of endogenous sphingolipids in rat serum and brain tissue and HT22 cells\u00a0and quantifies the changes in sphingolipid content in the serum and brain tissue of rats from the normal and pMCAO groups and in cells from the normal and OGD\/R groups. Using sphingosine (d17:1) as the internal standard, a chloroform: methanol (9:1) mixed system was used for protein precipitation and lipid extraction, followed by analysis by reversed-phase liquid chromatography coupled to triple quadrupole mass spectrometry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Based on absolute quantitative analysis of lipids in multiple biological samples, our results show that compared with those in the normal group, the contents of sphinganine (d16:0), sphinganine (d18:0), and phytosphingosine were significantly increased in the model group, except sphingosine-1-phosphate, which was decreased in various biological samples. The levels of each sphingolipid component in serum fluctuate with time.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This isotope-free and derivatization-free LC\u2012MS\/MS method can achieve absolute quantification of sphingolipids in biological samples, which may also help identify lipid biomarkers of cerebral ischemia.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s12944-022-01762-3","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T14:03:17Z","timestamp":1674655397000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Determination of endogenous sphingolipid content in stroke rats and HT22 cells subjected to oxygen-glucose deprivation by LC\u2012MS\/MS"],"prefix":"10.1186","volume":"22","author":[{"given":"Keqi","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,1,25]]},"reference":[{"issue":"8","key":"1762_CR1","doi-asserted-by":"publisher","first-page":"e254","DOI":"10.1161\/cir.0000000000000950","volume":"143","author":"SS Virani","year":"2021","unstructured":"Virani SS, Alonso A, Aparicio HJ, Benjamin EJ, Bittencourt MS, Callaway CW, Carson AP, Chamberlain AM, Cheng S, Delling FN, et al. Heart Disease and Stroke Statistics-2021 Update: A Report From the American Heart Association. Circulation. 2021;143(8):e254\u2013743. https:\/\/doi.org\/10.1161\/cir.0000000000000950.","journal-title":"Circulation"},{"issue":"4","key":"1762_CR2","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/s1474-4422(09)70025-0","volume":"8","author":"VL Feigin","year":"2009","unstructured":"Feigin VL, Lawes CM, Bennett DA, Barker-Collo SL, Parag V. Worldwide stroke incidence and early case fatality reported in 56 population-based studies: a systematic review. Lancet Neurol. 2009;8(4):355\u201369. https:\/\/doi.org\/10.1016\/s1474-4422(09)70025-0.","journal-title":"Lancet Neurol"},{"issue":"2","key":"1762_CR3","doi-asserted-by":"publisher","first-page":"2121","DOI":"10.4314\/ahs.v19i2.36","volume":"19","author":"Q Cui","year":"2019","unstructured":"Cui Q, Naikoo NA. Modifiable and non-modifiable risk factors in ischemic stroke: a meta-analysis. Afr Health Sci. 2019;19(2):2121\u20139. https:\/\/doi.org\/10.4314\/ahs.v19i2.36.","journal-title":"Afr Health Sci"},{"issue":"1","key":"1762_CR4","doi-asserted-by":"publisher","first-page":"E4","DOI":"10.3171\/2021.4.FOCUS21132","volume":"51","author":"S Kandregula","year":"2021","unstructured":"Kandregula S, Savardekar AR, Sharma P, McLarty J, Kosty J, Trosclair K, Cuellar H, Guthikonda B. Direct thrombectomy versus bridging thrombolysis with mechanical thrombectomy in middle cerebral artery stroke: a real-world analysis through National Inpatient Sample data. Neurosurg Focus. 2021;51(1):E4. https:\/\/doi.org\/10.3171\/2021.4.FOCUS21132.","journal-title":"Neurosurg Focus"},{"issue":"2","key":"1762_CR5","doi-asserted-by":"publisher","first-page":"107","DOI":"10.7417\/CT.2022.2403","volume":"173","author":"N Huu An","year":"2022","unstructured":"Huu An N, Dang Luu V, Duy Ton M, Anh Tuan T, Quang Anh N, Hoang Kien L, Tat Thien N, Viet Phuong D, Minh DN. Thrombectomy Alone versus Bridging Therapy in Acute Ischemic Stroke: Preliminary Results of an Experimental Trial. Clin Ter. 2022;173(2):107\u201314. https:\/\/doi.org\/10.7417\/CT.2022.2403.","journal-title":"Clin Ter"},{"key":"1762_CR6","doi-asserted-by":"publisher","first-page":"864618","DOI":"10.3389\/fmolb.2022.864618","volume":"9","author":"A Ouro","year":"2022","unstructured":"Ouro A, Correa-Paz C, Maqueda E, Custodia A, Aramburu-Nunez M, Romaus-Sanjurjo D, Posado-Fernandez A, Candamo-Lourido M, Alonso-Alonso ML, Hervella P, et al. Involvement of Ceramide Metabolism in Cerebral Ischemia. Front Mol Biosci. 2022;9:864618. https:\/\/doi.org\/10.3389\/fmolb.2022.864618.","journal-title":"Front Mol Biosci"},{"issue":"2","key":"1762_CR7","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1136\/jnnp-2020-324283","volume":"92","author":"A Chamorro","year":"2021","unstructured":"Chamorro A, Lo EH, Renu A, van Leyen K, Lyden PD. The future of neuroprotection in stroke. J Neurol Neurosurg Psychiatry. 2021;92(2):129\u201335. https:\/\/doi.org\/10.1136\/jnnp-2020-324283.","journal-title":"J Neurol Neurosurg Psychiatry"},{"issue":"1","key":"1762_CR8","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/s12944-019-0965-z","volume":"18","author":"G Hussain","year":"2019","unstructured":"Hussain G, Wang J, Rasul A, Anwar H, Imran A, Qasim M, Zafar S, Kamran SKS, Razzaq A, Aziz N, et al. Role of cholesterol and sphingolipids in brain development and neurological diseases. Lipids Health Dis. 2019;18(1):26. https:\/\/doi.org\/10.1186\/s12944-019-0965-z.","journal-title":"Lipids Health Dis"},{"issue":"1","key":"1762_CR9","doi-asserted-by":"publisher","first-page":"129","DOI":"10.3233\/ADR-210299","volume":"6","author":"JD Rudge","year":"2022","unstructured":"Rudge JD. A New Hypothesis for Alzheimer\u2019s Disease: The Lipid Invasion Model. J Alzheimers Dis Rep. 2022;6(1):129\u201361. https:\/\/doi.org\/10.3233\/ADR-210299.","journal-title":"J Alzheimers Dis Rep"},{"issue":"9","key":"1762_CR10","doi-asserted-by":"publisher","first-page":"5009","DOI":"10.3390\/ijms22095009","volume":"22","author":"M Hernaiz-Llorens","year":"2021","unstructured":"Hernaiz-Llorens M, Mart\u00ednez-M\u00e1rmol R, Rosell\u00f3-Busquets C, Soriano E. One Raft to Guide Them All, and in Axon Regeneration Inhibit Them. Int J Mol Sci. 2021;22(9):5009. https:\/\/doi.org\/10.3390\/ijms22095009.","journal-title":"Int J Mol Sci."},{"issue":"3","key":"1762_CR11","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1042\/EBC20190091","volume":"64","author":"A Gomez-Larrauri","year":"2020","unstructured":"Gomez-Larrauri A, Presa N, Dominguez-Herrera A, Ouro A, Trueba M, Gomez-Munoz A. Role of bioactive sphingolipids in physiology and pathology. Essays Biochem. 2020;64(3):579\u201389. https:\/\/doi.org\/10.1042\/EBC20190091.","journal-title":"Essays Biochem"},{"issue":"2","key":"1762_CR12","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1167\/iovs.09-3909","volume":"51","author":"CE Abrahan","year":"2010","unstructured":"Abrahan CE, Miranda GE, Agnolazza DL, Politi LE, Rotstein NP. Synthesis of sphingosine is essential for oxidative stress-induced apoptosis of photoreceptors. Invest Ophthalmol Vis Sci. 2010;51(2):1171\u201380. https:\/\/doi.org\/10.1167\/iovs.09-3909.","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"22","key":"1762_CR13","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.1002\/1873-3468.13933","volume":"594","author":"NJ Pyne","year":"2020","unstructured":"Pyne NJ, Pyne S. Recent advances in the role of sphingosine 1-phosphate in cancer. FEBS Lett. 2020;594(22):3583\u2013601. https:\/\/doi.org\/10.1002\/1873-3468.13933.","journal-title":"FEBS Lett"},{"issue":"7","key":"1762_CR14","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.1002\/jnr.21586","volume":"86","author":"IN Singh","year":"2008","unstructured":"Singh IN, Hall ED. Multifaceted roles of sphingosine-1-phosphate: how does this bioactive sphingolipid fit with acute neurological injury? J Neurosci Res. 2008;86(7):1419\u201333. https:\/\/doi.org\/10.1002\/jnr.21586.","journal-title":"J Neurosci Res"},{"issue":"9","key":"1762_CR15","doi-asserted-by":"publisher","first-page":"3470","DOI":"10.1021\/acs.jproteome.9b00370","volume":"18","author":"HC Chao","year":"2019","unstructured":"Chao HC, Lee TH, Chiang CS, Yang SY, Kuo CH, Tang SC. Sphingolipidomics Investigation of the Temporal Dynamics after Ischemic Brain Injury. J Proteome Res. 2019;18(9):3470\u20138. https:\/\/doi.org\/10.1021\/acs.jproteome.9b00370.","journal-title":"J Proteome Res"},{"key":"1762_CR16","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/bs.apcsb.2019.11.003","volume":"120","author":"OA Sukocheva","year":"2020","unstructured":"Sukocheva OA, Lukina E, McGowan E, Bishayee A. Sphingolipids as mediators of inflammation and novel therapeutic target in inflammatory bowel disease. Adv Protein Chem Struct Biol. 2020;120:123\u201358. https:\/\/doi.org\/10.1016\/bs.apcsb.2019.11.003.","journal-title":"Adv Protein Chem Struct Biol"},{"key":"1762_CR17","doi-asserted-by":"publisher","first-page":"112916","DOI":"10.1016\/j.jpba.2019.112916","volume":"179","author":"F Yin","year":"2020","unstructured":"Yin F, Keller J, Kraus D, Mangus H, Li F, Liu G. A double surrogate approach for the quantitation of 2-Hydroxyglutarate - An oncometabolite in human brain tumors via LC-MS\/MS. J Pharm Biomed Anal. 2020;179:112916. https:\/\/doi.org\/10.1016\/j.jpba.2019.112916.","journal-title":"J Pharm Biomed Anal"},{"key":"1762_CR18","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.jchromb.2019.02.023","volume":"1112","author":"HE Cho","year":"2019","unstructured":"Cho HE, Maurer BJ, Reynolds CP, Kang MH. Hydrophilic interaction liquid chromatography-tandem mass spectrometric approach for simultaneous determination of safingol and D-erythro-sphinganine in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;1112:16\u201323. https:\/\/doi.org\/10.1016\/j.jchromb.2019.02.023.","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"1762_CR19","doi-asserted-by":"publisher","first-page":"122041","DOI":"10.1016\/j.jchromb.2020.122041","volume":"1142","author":"Y Guan","year":"2020","unstructured":"Guan Y, Deng Q, Li G, Si L, Long L, Soleimani Damaneh M, Huang J. Development, validation and comparison of three LC-MS\/MS methods for determination of endogenous striatal oleoyl ethanolamine in mice. J Chromatogr B Analyt Technol Biomed Life Sci. 2020;1142:122041. https:\/\/doi.org\/10.1016\/j.jchromb.2020.122041.","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"1762_CR20","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.jpba.2016.06.017","volume":"128","author":"R Thakare","year":"2016","unstructured":"Thakare R, Chhonker YS, Gautam N, Alamoudi JA, Alnouti Y. Quantitative analysis of endogenous compounds. J Pharm Biomed Anal. 2016;128:426\u201337. https:\/\/doi.org\/10.1016\/j.jpba.2016.06.017.","journal-title":"J Pharm Biomed Anal"},{"issue":"7","key":"1762_CR21","doi-asserted-by":"publisher","first-page":"e4526","DOI":"10.1002\/bmc.4526","volume":"33","author":"D Xuejing","year":"2019","unstructured":"Xuejing D, Wenyu W, Hong W, Zhengrong Z, Li D, Jun F, Ran D, Feng L, Yan W, Xiang Z. UHPLC-MS\/MS analysis of sphingosine 1-phosphate in joint cavity dialysate and hemodialysis solution of adjuvant arthritis rats: Application to geniposide pharmacodynamic study. Biomed Chromatogr. 2019;33(7):e4526. https:\/\/doi.org\/10.1002\/bmc.4526.","journal-title":"Biomed Chromatogr"},{"issue":"1","key":"1762_CR22","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1186\/s12944-018-0917-z","volume":"17","author":"H Ozer","year":"2018","unstructured":"Ozer H, Aslan I, Oruc MT, Copelci Y, Afsar E, Kaya S, Aslan M. Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy. Lipids Health Dis. 2018;17(1):269. https:\/\/doi.org\/10.1186\/s12944-018-0917-z.","journal-title":"Lipids Health Dis"},{"issue":"1","key":"1762_CR23","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1161\/01.str.20.1.84","volume":"20","author":"EZ Longa","year":"1989","unstructured":"Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989;20(1):84\u201391. https:\/\/doi.org\/10.1161\/01.str.20.1.84.","journal-title":"Stroke"},{"issue":"5","key":"1762_CR24","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1194\/jlr.E400004-JLR200","volume":"46","author":"E Fahy","year":"2005","unstructured":"Fahy E, Subramaniam S, Brown HA, Glass CK, Merrill AH Jr, Murphy RC, Raetz CR, Russell DW, Seyama Y, Shaw W, et al. A comprehensive classification system for lipids. J Lipid Res. 2005;46(5):839\u201361. https:\/\/doi.org\/10.1194\/jlr.E400004-JLR200.","journal-title":"J Lipid Res"},{"issue":"1","key":"1762_CR25","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1186\/s12944-022-01707-w","volume":"21","author":"B Gao","year":"2022","unstructured":"Gao B, Li X, Chen W, Wang S, He J, Liu Y, Ding C, Chen X. The associations between lipid profiles and visceral obesity among gastrointestinal cancer patients: a cross-sectional study. Lipids Health Dis. 2022;21(1):102. https:\/\/doi.org\/10.1186\/s12944-022-01707-w.","journal-title":"Lipids Health Dis"},{"issue":"10","key":"1762_CR26","doi-asserted-by":"publisher","first-page":"2369","DOI":"10.1016\/j.neurobiolaging.2014.02.025","volume":"35","author":"SM Lam","year":"2014","unstructured":"Lam SM, Wang Y, Duan X, Wenk MR, Kalaria RN, Chen CP, Lai MK, Shui G. Brain lipidomes of subcortical ischemic vascular dementia and mixed dementia. Neurobiol Aging. 2014;35(10):2369\u201381. https:\/\/doi.org\/10.1016\/j.neurobiolaging.2014.02.025.","journal-title":"Neurobiol Aging"},{"issue":"12","key":"1762_CR27","doi-asserted-by":"publisher","first-page":"e12008","DOI":"10.7759\/cureus.12008","volume":"12","author":"M Agarwal","year":"2020","unstructured":"Agarwal M, Khan S. Plasma Lipids as Biomarkers for Alzheimer\u2019s Disease: A Systematic Review. Cureus. 2020;12(12):e12008. https:\/\/doi.org\/10.7759\/cureus.12008.","journal-title":"Cureus"},{"issue":"4","key":"1762_CR28","doi-asserted-by":"publisher","first-page":"100191","DOI":"10.1016\/j.jlr.2022.100191","volume":"63","author":"SC Spaulding","year":"2022","unstructured":"Spaulding SC, Bollag WB. The role of lipid second messengers in aldosterone synthesis and secretion. J Lipid Res. 2022;63(4):100191. https:\/\/doi.org\/10.1016\/j.jlr.2022.100191.","journal-title":"J Lipid Res"},{"key":"1762_CR29","doi-asserted-by":"publisher","first-page":"110566","DOI":"10.1016\/j.foodres.2021.110566","volume":"147","author":"W Li","year":"2021","unstructured":"Li W, Belwal T, Li L, Xu Y, Liu J, Zou L, Luo Z. Sphingolipids in foodstuff: Compositions, distribution, digestion, metabolism and health effects - A comprehensive review. Food Res Int. 2021;147:110566. https:\/\/doi.org\/10.1016\/j.foodres.2021.110566.","journal-title":"Food Res Int"},{"issue":"26","key":"1762_CR30","doi-asserted-by":"publisher","first-page":"2696","DOI":"10.1016\/j.jchromb.2008.12.057","volume":"877","author":"CA Haynes","year":"2009","unstructured":"Haynes CA, Allegood JC, Park H, Sullards MC. Sphingolipidomics: methods for the comprehensive analysis of sphingolipids. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(26):2696\u2013708. https:\/\/doi.org\/10.1016\/j.jchromb.2008.12.057.","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"1762_CR31","doi-asserted-by":"publisher","first-page":"950","DOI":"10.3389\/fneur.2019.00950","volume":"10","author":"I Azizkhanian","year":"2019","unstructured":"Azizkhanian I, Sheth SA, Iavarone AT, Lee S, Kakarla V, Hinman JD. Plasma Lipid Profiling Identifies Biomarkers of Cerebral Microvascular Disease. Front Neurol. 2019;10:950. https:\/\/doi.org\/10.3389\/fneur.2019.00950.","journal-title":"Front Neurol"},{"key":"1762_CR32","doi-asserted-by":"publisher","first-page":"175628641876983","DOI":"10.1177\/1756286418769830","volume":"11","author":"S Luger","year":"2018","unstructured":"Luger S, Schwebler A, Vutukuri R, Bouzas NF, Labocha S, Schreiber Y, Brunkhorst R, Steinmetz H, Pfeilschifter J, Pfeilschifter W. Beta adrenoceptor blockade ameliorates impaired glucose tolerance and alterations of the cerebral ceramide metabolism in an experimental model of ischemic stroke. Ther Adv Neurol Disord. 2018;11:1756286418769830. https:\/\/doi.org\/10.1177\/1756286418769830.","journal-title":"Ther Adv Neurol Disord"},{"issue":"13","key":"1762_CR33","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1002\/mds.25945","volume":"29","author":"T Pringsheim","year":"2014","unstructured":"Pringsheim T, Jette N, Frolkis A, Steeves TD. The prevalence of Parkinson\u2019s disease: a systematic review and meta-analysis. Mov Disord. 2014;29(13):1583\u201390. https:\/\/doi.org\/10.1002\/mds.25945.","journal-title":"Mov Disord"},{"key":"1762_CR34","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.cca.2021.06.020","volume":"521","author":"S Lu","year":"2021","unstructured":"Lu S, She M, Zeng Q, Yi G, Zhang J. Sphingosine 1-phosphate and its receptors in ischemia. Clin Chim Acta. 2021;521:25\u201333. https:\/\/doi.org\/10.1016\/j.cca.2021.06.020.","journal-title":"Clin Chim Acta"},{"key":"1762_CR35","doi-asserted-by":"publisher","first-page":"117542","DOI":"10.1016\/j.lfs.2020.117542","volume":"249","author":"M Han","year":"2020","unstructured":"Han M, Sun T, Chen H, Han M, Wang D. Potential sphingosine-1-phosphate-related therapeutic targets in the treatment of cerebral ischemia reperfusion injury. Life Sci. 2020;249:117542. https:\/\/doi.org\/10.1016\/j.lfs.2020.117542.","journal-title":"Life Sci"},{"key":"1762_CR36","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.jbior.2016.10.002","volume":"63","author":"N Coant","year":"2017","unstructured":"Coant N, Sakamoto W, Mao C, Hannun YA. Ceramidases, roles in sphingolipid metabolism and in health and disease. Adv Biol Regul. 2017;63:122\u201331. https:\/\/doi.org\/10.1016\/j.jbior.2016.10.002.","journal-title":"Adv Biol Regul"},{"issue":"3","key":"1762_CR37","doi-asserted-by":"publisher","first-page":"213","DOI":"10.14670\/hh-18-428","volume":"37","author":"Y Qiu","year":"2022","unstructured":"Qiu Y, Shen J, Jiang W, Yang Y, Liu X, Zeng Y. Sphingosine 1-phosphate and its regulatory role in vascular endothelial cells. Histol Histopathol. 2022;37(3):213\u201325. https:\/\/doi.org\/10.14670\/hh-18-428.","journal-title":"Histol Histopathol."},{"issue":"3","key":"1762_CR38","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1161\/circresaha.120.316711","volume":"128","author":"A Nitzsche","year":"2021","unstructured":"Nitzsche A, Poittevin M, Benarab A, Bonnin P, Faraco G, Uchida H, Favre J, Garcia-Bonilla L, Garcia MCL, L\u00e9ger PL, et al. Endothelial S1P(1) Signaling Counteracts Infarct Expansion in Ischemic Stroke. Circ Res. 2021;128(3):363\u201382. https:\/\/doi.org\/10.1161\/circresaha.120.316711.","journal-title":"Circ Res"},{"issue":"1","key":"1762_CR39","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s12017-020-08632-0","volume":"23","author":"XY Chua","year":"2021","unstructured":"Chua XY, Ho LTY, Xiang P, Chew WS, Lam BWS, Chen CP, Ong WY, Lai MKP, Herr DR. Preclinical and Clinical Evidence for the Involvement of Sphingosine 1-Phosphate Signaling in the Pathophysiology of Vascular Cognitive Impairment. Neuromolecular Med. 2021;23(1):47\u201367. https:\/\/doi.org\/10.1007\/s12017-020-08632-0.","journal-title":"Neuromolecular Med"},{"key":"1762_CR40","doi-asserted-by":"publisher","first-page":"106436","DOI":"10.1016\/j.prostaglandins.2020.106436","volume":"149","author":"Z Raza","year":"2020","unstructured":"Raza Z, Saleem U, Naureen Z. Sphingosine 1-phosphate signaling in ischemia and reperfusion injury. Prostaglandins Other Lipid Mediat. 2020;149:106436. https:\/\/doi.org\/10.1016\/j.prostaglandins.2020.106436.","journal-title":"Prostaglandins Other Lipid Mediat"},{"issue":"12","key":"1762_CR41","doi-asserted-by":"publisher","first-page":"3901","DOI":"10.1161\/strokeaha.120.033414","volume":"52","author":"E Schwedhelm","year":"2021","unstructured":"Schwedhelm E, Schwieren L, Tiedt S, von Lucadou M, Gloyer NO, B\u00f6ger R, Magnus T, Daum G, Thomalla G, Gerloff C, Choe CU. Serum Sphingosine-1-Phosphate Levels Are Associated With Severity and Outcome in Patients With Cerebral Ischemia. Stroke. 2021;52(12):3901\u20137. https:\/\/doi.org\/10.1161\/strokeaha.120.033414.","journal-title":"Stroke"},{"key":"1762_CR42","doi-asserted-by":"publisher","first-page":"720672","DOI":"10.3389\/fphys.2021.720672","volume":"12","author":"F Yu","year":"2021","unstructured":"Yu F, Feng X, Li X, Liu Z, Liao D, Luo Y, Wei M, Huang Q, Zhang L, Xia J. Association of Plasma Metabolic Biomarker Sphingosine-1-Phosphate With Cerebral Collateral Circulation in Acute Ischemic Stroke. Front Physiol. 2021;12:720672. https:\/\/doi.org\/10.3389\/fphys.2021.720672.","journal-title":"Front Physiol"},{"issue":"1 Pt 1","key":"1762_CR43","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.jfma.2021.01.006","volume":"121","author":"TH Lee","year":"2022","unstructured":"Lee TH, Cheng CN, Chao HC, Lee CH, Kuo CH, Tang SC, Jeng JS. Plasma ceramides are associated with outcomes in acute ischemic stroke patients. J Formos Med Assoc. 2022;121(1 Pt 1):43\u201350. https:\/\/doi.org\/10.1016\/j.jfma.2021.01.006.","journal-title":"J Formos Med Assoc"},{"issue":"2","key":"1762_CR44","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1007\/s12028-021-01338-6","volume":"36","author":"HH Zhang","year":"2022","unstructured":"Zhang HH, Wang L, Zhang W, Wan Z. The Role of Sphingomyelin Metabolism in the Protection of Rat Brain Microvascular Endothelial Cells by Mild Hypothermia. Neurocrit Care. 2022;36(2):546\u201359. https:\/\/doi.org\/10.1007\/s12028-021-01338-6.","journal-title":"Neurocrit Care"},{"issue":"14","key":"1762_CR45","doi-asserted-by":"publisher","first-page":"7468","DOI":"10.3390\/ijms22147468","volume":"22","author":"A Kov\u00e1\u010dik","year":"2021","unstructured":"Kov\u00e1\u010dik A, Pullmannov\u00e1 P, Op\u00e1lka L, \u0160ilarov\u00e1 M, Maixner J, V\u00e1vrov\u00e1 K. Effects of (R)- and (S)-\u03b1-Hydroxylation of Acyl Chains in Sphingosine, Dihydrosphingosine, and Phytosphingosine Ceramides on Phase Behavior and Permeability of Skin Lipid Models. Int J Mol Sci. 2021;22(14):7468. https:\/\/doi.org\/10.3390\/ijms22147468.","journal-title":"Int J Mol Sci."},{"key":"1762_CR46","doi-asserted-by":"publisher","DOI":"10.1136\/neurintsurg-2022-019047","author":"W Li","year":"2022","unstructured":"Li W, Bai X, Hao J, Xu X, Lin F, Jiang Q, Ding C, Dai G, Peng F, Zhang M, et al. Thrombosis origin identification of cardioembolism and large artery atherosclerosis by distinct metabolites. J Neurointerv Surg. 2022. https:\/\/doi.org\/10.1136\/neurintsurg-2022-019047.","journal-title":"J Neurointerv Surg"},{"issue":"4","key":"1762_CR47","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1111\/jnc.12836","volume":"131","author":"FD Testai","year":"2014","unstructured":"Testai FD, Kilkus JP, Berdyshev E, Gorshkova I, Natarajan V, Dawson G. Multiple sphingolipid abnormalities following cerebral microendothelial hypoxia. J Neurochem. 2014;131(4):530\u201340. https:\/\/doi.org\/10.1111\/jnc.12836.","journal-title":"J Neurochem"},{"issue":"11","key":"1762_CR48","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.1038\/s41431-019-0444-z","volume":"27","author":"V Dolgin","year":"2019","unstructured":"Dolgin V, Straussberg R, Xu R, Mileva I, Yogev Y, Khoury R, Konen O, Barhum Y, Zvulunov A, Mao C, Birk OS. DEGS1 variant causes neurological disorder. Eur J Hum Genet. 2019;27(11):1668\u201376. https:\/\/doi.org\/10.1038\/s41431-019-0444-z.","journal-title":"Eur J Hum Genet"},{"issue":"10","key":"1762_CR49","doi-asserted-by":"publisher","first-page":"4519","DOI":"10.1039\/d0fo02845b","volume":"12","author":"R Wang","year":"2021","unstructured":"Wang R, Liu S, Liu T, Wu J, Zhang H, Sun Z, Liu Z. Mass spectrometry-based serum lipidomics strategy to explore the mechanism of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves in the treatment of ischemic stroke. Food Funct. 2021;12(10):4519\u201334. https:\/\/doi.org\/10.1039\/d0fo02845b.","journal-title":"Food Funct."},{"issue":"1","key":"1762_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0005-2760(70)90086-x","volume":"218","author":"NG Baz\u00e1n Jr","year":"1970","unstructured":"Baz\u00e1n NG Jr. Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brain. Biochim Biophys Acta. 1970;218(1):1\u201310. https:\/\/doi.org\/10.1016\/0005-2760(70)90086-x.","journal-title":"Biochim Biophys Acta"},{"issue":"4","key":"1762_CR51","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1111\/1753-0407.13262","volume":"14","author":"M Brusatori","year":"2022","unstructured":"Brusatori M, Wood MH, Tucker SC, Maddipati KR, Koya SK, Auner GW, Honn KV, Seyoum B. Ceramide changes in abdominal subcutaneous and visceral adipose tissue among diabetic and nondiabetic patients. J Diabetes. 2022;14(4):271\u201381. https:\/\/doi.org\/10.1111\/1753-0407.13262.","journal-title":"J Diabetes"},{"issue":"9","key":"1762_CR52","doi-asserted-by":"publisher","first-page":"459","DOI":"10.3390\/biom9090459","volume":"9","author":"G Brogden","year":"2019","unstructured":"Brogden G, Husein DM, Steinberg P, Naim HY. Isolation and Quantification of Sphingosine and Sphinganine from Rat Serum Revealed Gender Differences. Biomolecules. 2019;9(9):459. https:\/\/doi.org\/10.3390\/biom9090459.","journal-title":"Biomolecules."},{"key":"1762_CR53","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1007\/978-1-0716-2655-9_13","volume":"2561","author":"J Martinez-Gardeazabal","year":"2023","unstructured":"Martinez-Gardeazabal J, Moreno-Rodriguez M, de San Roman EG, Abad B, Manuel I, Rodriguez-Puertas R. Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer\u2019s Research. Methods Mol Biol. 2023;2561:245\u201359. https:\/\/doi.org\/10.1007\/978-1-0716-2655-9_13.","journal-title":"Methods Mol Biol"},{"key":"1762_CR54","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1007\/978-1-0716-2565-1_25","volume":"2546","author":"BB Stauffer","year":"2022","unstructured":"Stauffer BB, Yu C. Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography Tandem Mass Spectrometry. Methods Mol Biol. 2022;2546:271\u201384. https:\/\/doi.org\/10.1007\/978-1-0716-2565-1_25.","journal-title":"Methods Mol Biol"},{"key":"1762_CR55","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1007\/978-1-0716-2655-9_12","volume":"2561","author":"XY Chua","year":"2023","unstructured":"Chua XY, Huang R, Herr D, Lai MKP, Wenk MR, Torta F. Mass Spectrometry Analysis of the Human Brain Sphingolipidome. Methods Mol Biol. 2023;2561:233\u201343. https:\/\/doi.org\/10.1007\/978-1-0716-2655-9_12.","journal-title":"Methods Mol Biol"},{"key":"1762_CR56","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.chroma.2013.05.019","volume":"1298","author":"S Chen","year":"2013","unstructured":"Chen S, Hoene M, Li J, Li Y, Zhao X, H\u00e4ring HU, Schleicher ED, Weigert C, Xu G, Lehmann R. Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography\/mass spectrometry. J Chromatogr A. 2013;1298:9\u201316. https:\/\/doi.org\/10.1016\/j.chroma.2013.05.019.","journal-title":"J Chromatogr A"},{"issue":"1","key":"1762_CR57","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.jpha.2021.01.001","volume":"12","author":"H Zhang","year":"2022","unstructured":"Zhang H, Li Y, Li Z, Lam CW, Zhu P, Wang C, Zhou H, Zhang W. MTBSTFA derivatization-LC-MS\/MS approach for the quantitative analysis of endogenous nucleotides in human colorectal carcinoma cells. J Pharm Anal. 2022;12(1):77\u201386. https:\/\/doi.org\/10.1016\/j.jpha.2021.01.001.","journal-title":"J Pharm Anal"},{"issue":"8","key":"1762_CR58","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1016\/j.bbalip.2017.03.002","volume":"1862","author":"J Brandsma","year":"2017","unstructured":"Brandsma J, Bailey AP, Koster G, Gould AP, Postle AD. Stable isotope analysis of dynamic lipidomics. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(8):792\u20136. https:\/\/doi.org\/10.1016\/j.bbalip.2017.03.002.","journal-title":"Biochim Biophys Acta Mol Cell Biol Lipids"}],"container-title":["Lipids in Health and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12944-022-01762-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12944-022-01762-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12944-022-01762-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T14:05:25Z","timestamp":1674655525000},"score":1,"resource":{"primary":{"URL":"https:\/\/lipidworld.biomedcentral.com\/articles\/10.1186\/s12944-022-01762-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,25]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["1762"],"URL":"https:\/\/doi.org\/10.1186\/s12944-022-01762-3","relation":{},"ISSN":["1476-511X"],"issn-type":[{"value":"1476-511X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,25]]},"assertion":[{"value":"14 October 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 January 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This animal experiment was approved by the Laboratory Animal Ethics Committee of Guangzhou University of Chinese Medicine. (Record number: 20210323002).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors provide formal consent to publish the work.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13"}}