{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:53:11Z","timestamp":1770339191080,"version":"3.49.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":28,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Natural Science Foundation of Jilin Province","award":["20240101272JC"],"award-info":[{"award-number":["20240101272JC"]}]},{"name":"the National Key R&D Program of China","award":["No. 2022YFC2504200"],"award-info":[{"award-number":["No. 2022YFC2504200"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 82270960"],"award-info":[{"award-number":["No. 82270960"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Science & Technology Development Talent Project of Jilin Financial Department, Jilin, China","award":["No. JCSZ2021893-35"],"award-info":[{"award-number":["No. JCSZ2021893-35"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Lipids Health Dis"],"DOI":"10.1186\/s12944-025-02853-7","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T10:30:52Z","timestamp":1767868252000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alternative polyadenylation mediated the downregulation of lysophosphatidylglycerol acyltransferase 1 in metabolic dysfunction-associated steatotic liver disease"],"prefix":"10.1186","volume":"25","author":[{"given":"Wei","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aowen","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaoran","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Shu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianli","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baiyu","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjin","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengwen","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"2853_CR1","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1038\/s41576-019-0145-z","volume":"20","author":"AJ Gruber","year":"2019","unstructured":"Gruber AJ, Zavolan M. Alternative cleavage and polyadenylation in health and disease. Nat Rev Genet. 2019;20:599\u2013614.","journal-title":"Nat Rev Genet"},{"key":"2853_CR2","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1038\/s41580-021-00356-8","volume":"22","author":"S Das","year":"2021","unstructured":"Das S, Vera M, Gandin V, Singer RH, Tutucci E. Intracellular mRNA transport and localized translation. Nat Rev Mol Cell Biol. 2021;22:483\u2013504.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2853_CR3","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1038\/nrm.2016.116","volume":"18","author":"B Tian","year":"2017","unstructured":"Tian B, Manley JL. Alternative polyadenylation of mRNA precursors. Nat Rev Mol Cell Biol. 2017;18:18\u201330.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2853_CR4","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/s41575-020-00406-0","volume":"18","author":"MF Abdelmalek","year":"2021","unstructured":"Abdelmalek MF. Nonalcoholic fatty liver disease: another leap forward. Nat Rev Gastroenterol Hepatol. 2021;18:85\u20136.","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"2853_CR5","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.canlet.2017.09.026","volume":"410","author":"M Sun","year":"2017","unstructured":"Sun M, Ding J, Li D, Yang G, Cheng Z, Zhu Q. NUDT21 regulates 3\u2019-UTR length and microRNA-mediated gene Silencing in hepatocellular carcinoma. Cancer Lett. 2017;410:158\u201368.","journal-title":"Cancer Lett"},{"key":"2853_CR6","doi-asserted-by":"publisher","first-page":"1687","DOI":"10.1001\/jama.2023.17935","volume":"330","author":"PB Leung","year":"2023","unstructured":"Leung PB, Davis AM, Kumar S. Diagnosis and management of nonalcoholic fatty liver disease. JAMA. 2023;330:1687\u20138.","journal-title":"JAMA"},{"key":"2853_CR7","doi-asserted-by":"publisher","first-page":"1607","DOI":"10.1136\/gutjnl-2023-329998","volume":"72","author":"T Marjot","year":"2023","unstructured":"Marjot T, Tomlinson JW, Hodson L, Ray DW. Timing of energy intake and the therapeutic potential of intermittent fasting and time-restricted eating in NAFLD. Gut. 2023;72:1607\u201319.","journal-title":"Gut"},{"key":"2853_CR8","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1053\/j.gastro.2016.05.051","volume":"151","author":"A Teufel","year":"2016","unstructured":"Teufel A, Itzel T, Erhart W, Brosch M, Wang XY, Kim YO, et al. Comparison of gene expression patterns between mouse models of nonalcoholic fatty liver disease and liver tissues from patients. Gastroenterology. 2016;151:513-25.e0.","journal-title":"Gastroenterology."},{"key":"2853_CR9","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/s41574-018-0096-z","volume":"15","author":"FJ Gonzalez","year":"2018","unstructured":"Gonzalez FJ, Xie C, Jiang C. The role of hypoxia-inducible factors in metabolic diseases. Nat Rev Endocrinol. 2018;15:21\u201332.","journal-title":"Nat Rev Endocrinol"},{"key":"2853_CR10","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.jhep.2015.02.019","volume":"63","author":"S Francque","year":"2015","unstructured":"Francque S, Verrijken A, Caron S, Prawitt J, Paumelle R, Derudas B, et al. PPAR\u03b1 gene expression correlates with severity and histological treatment response in patients with non-alcoholic steatohepatitis. J Hepatol. 2015;63:164\u201373.","journal-title":"J Hepatol"},{"key":"2853_CR11","doi-asserted-by":"publisher","first-page":"3379","DOI":"10.1093\/nar\/gkac165","volume":"50","author":"AM Jobbins","year":"2022","unstructured":"Jobbins AM, Haberman N, Artigas N, Amourda C, Paterson HAB, Yu S, et al. Dysregulated RNA polyadenylation contributes to metabolic impairment in non-alcoholic fatty liver disease. Nucleic Acids Res. 2022;50:3379\u201393.","journal-title":"Nucleic Acids Res"},{"key":"2853_CR12","doi-asserted-by":"publisher","first-page":"4050","DOI":"10.1038\/s41467-024-48254-9","volume":"15","author":"MM Fansler","year":"2024","unstructured":"Fansler MM, Mitschka S, Mayr C. Quantifying 3\u2019UTR length from scRNA-seq data reveals changes independent of gene expression. Nat Commun. 2024;15:4050.","journal-title":"Nat Commun"},{"key":"2853_CR13","doi-asserted-by":"publisher","first-page":"5681","DOI":"10.7150\/ijbs.65044","volume":"18","author":"T Fang","year":"2022","unstructured":"Fang T, Wang H, Pan X, Little PJ, Xu S, Weng J. Mouse models of nonalcoholic fatty liver disease (NAFLD): pathomechanisms and pharmacotherapies. Int J Biol Sci. 2022;18:5681\u201397.","journal-title":"Int J Biol Sci"},{"key":"2853_CR14","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/BF02623304","volume":"22","author":"BL Kreamer","year":"1986","unstructured":"Kreamer BL, Staecker JL, Sawada N, Sattler GL, Hsia MT, Pitot HC. Use of a low-speed, iso-density Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. Vitro Cell Dev Biol. 1986;22:201\u201311.","journal-title":"Vitro Cell Dev Biol"},{"key":"2853_CR15","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1083\/jcb.43.3.506","volume":"43","author":"MN Berry","year":"1969","unstructured":"Berry MN, Friend DS. High-yield Preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969;43:506\u201320.","journal-title":"J Cell Biol"},{"key":"2853_CR16","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/s41587-023-01767-y","volume":"42","author":"Y Hao","year":"2023","unstructured":"Hao Y, Stuart T, Kowalski MH, Choudhary S, Hoffman P, Hartman A, et al. Dictionary learning for integrative, multimodal and scalable single-cell analysis. Nat Biotechnol. 2023;42:293\u2013304.","journal-title":"Nat Biotechnol"},{"key":"2853_CR17","doi-asserted-by":"publisher","first-page":"979","DOI":"10.12688\/f1000research.73600.1","volume":"10","author":"PL Germain","year":"2021","unstructured":"Germain PL, Lun A, Meixide CG, Macnair W, Robinson MD. Doublet identification in single-cell sequencing data using ScDblFinder. F1000Res. 2021;10:979.","journal-title":"F1000Res"},{"key":"2853_CR18","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/s41590-018-0276-y","volume":"20","author":"D Aran","year":"2019","unstructured":"Aran D, Looney AP, Liu L, Wu E, Fong V, Hsu A, et al. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nat Immunol. 2019;20:163\u201372.","journal-title":"Nat Immunol"},{"key":"2853_CR19","doi-asserted-by":"publisher","first-page":"e66","DOI":"10.1093\/nar\/gkac167","volume":"50","author":"R Zhou","year":"2022","unstructured":"Zhou R, Xiao X, He P, Zhao Y, Xu M, Zheng X, et al. SCAPE: a mixture model revealing single-cell polyadenylation diversity and cellular dynamics during cell differentiation and reprogramming. Nucleic Acids Res. 2022;50:e66.","journal-title":"Nucleic Acids Res"},{"key":"2853_CR20","doi-asserted-by":"publisher","first-page":"W317","DOI":"10.1093\/nar\/gkab447","volume":"49","author":"D Bu","year":"2021","unstructured":"Bu D, Luo H, Huo P, Wang Z, Zhang S, He Z, et al. KOBAS-i: intelligent prioritization and exploratory visualization of biological functions for gene enrichment analysis. Nucleic Acids Res. 2021;49:W317\u201325.","journal-title":"Nucleic Acids Res"},{"key":"2853_CR21","doi-asserted-by":"publisher","first-page":"e107","DOI":"10.1002\/imt2.107","volume":"2","author":"S Chen","year":"2023","unstructured":"Chen S. Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using Fastp. Imeta. 2023;2:e107.","journal-title":"Imeta"},{"key":"2853_CR22","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","volume":"37","author":"D Kim","year":"2019","unstructured":"Kim D, Paggi JM, Park C, Bennett C, Salzberg SL. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat Biotechnol. 2019;37:907\u201315.","journal-title":"Nat Biotechnol"},{"key":"2853_CR23","doi-asserted-by":"publisher","first-page":"5","DOI":"10.6026\/97320630002005","volume":"2","author":"L Kumar","year":"2007","unstructured":"Kumar L, Futschik ME. Mfuzz: a software package for soft clustering of microarray data. Bioinformation. 2007;2:5\u20137.","journal-title":"Bioinformation"},{"key":"2853_CR24","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1038\/s41588-021-00864-5","volume":"53","author":"L Li","year":"2021","unstructured":"Li L, Huang KL, Gao Y, Cui Y, Wang G, Elrod ND, et al. An atlas of alternative polyadenylation quantitative trait loci contributing to complex trait and disease heritability. Nat Genet. 2021;53:994\u20131005.","journal-title":"Nat Genet"},{"key":"2853_CR25","doi-asserted-by":"publisher","first-page":"e1692","DOI":"10.1002\/wrna.1692","volume":"13","author":"NK Mohanan","year":"2022","unstructured":"Mohanan NK, Shaji F, Koshre GR, Laishram RS. Alternative polyadenylation: an enigma of transcript length variation in health and disease. Wiley Interdiscip Rev RNA. 2022;13:e1692.","journal-title":"Wiley Interdiscip Rev RNA"},{"key":"2853_CR26","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1038\/s42255-024-01038-3","volume":"6","author":"S Jiang","year":"2024","unstructured":"Jiang S, Yuan T, Rosenberger FA, Mourier A, Dragano NRV, Kremer LS, et al. Inhibition of mammalian MtDNA transcription acts paradoxically to reverse diet-induced hepatosteatosis and obesity. Nat Metab. 2024;6:1024\u201335.","journal-title":"Nat Metab"},{"key":"2853_CR27","doi-asserted-by":"publisher","first-page":"111951","DOI":"10.1016\/j.isci.2025.111951","volume":"28","author":"Q Su","year":"2025","unstructured":"Su Q, Kim SY, Adewale F, Zhou Y, Aldler C, Ni M, et al. Erratum: Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver. iScience. 2025;28:111951.","journal-title":"iScience"},{"key":"2853_CR28","doi-asserted-by":"publisher","first-page":"e447","DOI":"10.1038\/cddis.2012.185","volume":"3","author":"A Badmann","year":"2012","unstructured":"Badmann A, Langsch S, Keogh A, Brunner T, Kaufmann T, Corazza N. TRAIL enhances paracetamol-induced liver sinusoidal endothelial cell death in a Bim- and Bid-dependent manner. Cell Death Dis. 2012;3:e447.","journal-title":"Cell Death Dis"},{"key":"2853_CR29","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1016\/j.jcmgh.2019.02.002","volume":"7","author":"X Zhang","year":"2019","unstructured":"Zhang X, Zhang J, Sun H, Liu X, Zheng Y, Xu D, et al. Defective phosphatidylglycerol remodeling causes hepatopathy, linking mitochondrial dysfunction to hepatosteatosis. Cell Mol Gastroenterol Hepatol. 2019;7:763\u201381.","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"2853_CR30","doi-asserted-by":"publisher","first-page":"113376","DOI":"10.1016\/j.celrep.2023.113376","volume":"42","author":"VF Monteiro-Cardoso","year":"2023","unstructured":"Monteiro-Cardoso VF, Giordano F. New roles of LPGAT1: from mitochondrial import of phosphatidylglycerol to MEGDEL disease. Cell Rep. 2023;42:113376.","journal-title":"Cell Rep"},{"key":"2853_CR31","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1038\/nm.3200","volume":"19","author":"J Soh","year":"2013","unstructured":"Soh J, Iqbal J, Queiroz J, Fernandez-Hernando C, Hussain MM. MicroRNA-30c reduces hyperlipidemia and atherosclerosis in mice by decreasing lipid synthesis and lipoprotein secretion. Nat Med. 2013;19:892\u2013900.","journal-title":"Nat Med"},{"key":"2853_CR32","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1002\/oby.20243","volume":"21","author":"MT Traurig","year":"2013","unstructured":"Traurig MT, Orczewska JI, Ortiz DJ, Bian L, Marinelarena AM, Kobes S, et al. Evidence for a role of LPGAT1 in influencing BMI and percent body fat in native Americans. Obes (Silver Spring). 2013;21:193\u2013202.","journal-title":"Obes (Silver Spring)"}],"container-title":["Lipids in Health and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12944-025-02853-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12944-025-02853-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12944-025-02853-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:05:04Z","timestamp":1770293104000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12944-025-02853-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":32,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["2853"],"URL":"https:\/\/doi.org\/10.1186\/s12944-025-02853-7","relation":{},"ISSN":["1476-511X"],"issn-type":[{"value":"1476-511X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]},"assertion":[{"value":"16 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were conducted in accordance with institutional guidelines and were approved by the Experimental Animal Ethics Committee of Jilin University. The human transcriptomic datasets analyzed in this study were obtained from public databases (mice: GSE239747; human: GSE135251), and appropriate informed consent and ethical approval were obtained from all original studies.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"42"}}