{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:11:15Z","timestamp":1793315475522,"version":"4.5.0"},"reference-count":69,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":38,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Wuhan Outstanding Young Talents Project and Open Fund of Hubei Provincial Key Laboratory - Pilot Project","award":["2024KFZ020"],"award-info":[{"award-number":["2024KFZ020"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372093"],"award-info":[{"award-number":["82372093"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chinese Medicine Research Project of Hubei Provincial Administration of Traditional Chinese Medicine","award":["ZY2023Q034"],"award-info":[{"award-number":["ZY2023Q034"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Nanobiotechnol"],"DOI":"10.1186\/s12951-026-04237-0","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T01:43:32Z","timestamp":1772761412000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Matrix micro\/nano-topography drives oncogenic signaling and drug response in a 3D osteosarcoma model"],"prefix":"10.1186","volume":"24","author":[{"given":"Mei-Ling","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Chao","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Lin","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Qiu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"issue":"10","key":"4237_CR1","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1038\/s41571-021-00519-8","volume":"18","author":"J Gill","year":"2021","unstructured":"Gill J, Gorlick R. Advancing therapy for osteosarcoma[J]. Nat Rev Clin Oncol. 2021;18(10):609\u201324.","journal-title":"Nat Rev Clin Oncol"},{"key":"4237_CR2","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1093\/annonc\/mdq276","volume":"21","author":"J Ritter","year":"2010","unstructured":"Ritter J, Bielack SS. Osteosarcoma[J]. Ann Oncol. 2010;21:320\u20135.","journal-title":"Ann Oncol"},{"issue":"22","key":"4237_CR3","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1056\/NEJMra2103423","volume":"385","author":"PS Meltzer","year":"2021","unstructured":"Meltzer PS, Helman LJ. New Horizons in the Treatment of Osteosarcoma[J]. N Engl J Med. 2021;385(22):2066\u201376.","journal-title":"N Engl J Med"},{"key":"4237_CR4","doi-asserted-by":"crossref","unstructured":"Beird HC, Bielack SS, Flanagan AM, Gill J, Heymann D, Janeway KA, Livingston JA, Roberts RD, Strauss SJ, Gorlick R. Osteosarcoma[J]. Nat Rev Dis Primers. 2022:8(1):77.","DOI":"10.1038\/s41572-022-00409-y"},{"issue":"2","key":"4237_CR5","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1038\/nbt0204-151","volume":"22","author":"SG Zhang","year":"2004","unstructured":"Zhang SG. Beyond the Petri dish[J]. Nat Biotechnol. 2004;22(2):151\u20132.","journal-title":"Nat Biotechnol"},{"issue":"12","key":"4237_CR6","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1038\/nrm3904","volume":"15","author":"C Bonnans","year":"2014","unstructured":"Bonnans C, Chou J, Werb Z. Remodelling the extracellular matrix in development and disease[J]. Nat Rev Mol Cell Biol. 2014;15(12):786\u2013801.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"4237_CR7","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.addr.2015.11.001","volume":"97","author":"AD Theocharis","year":"2016","unstructured":"Theocharis AD, Skandalis SS, Gialeli C, Karamanos NK. Extracellular matrix structure[J]. Adv Drug Delivery Rev. 2016;97:4\u201327.","journal-title":"Adv Drug Delivery Rev"},{"issue":"5957","key":"4237_CR8","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1126\/science.1176009","volume":"326","author":"H Ro","year":"2009","unstructured":"Ro H. The extracellular matrix: not just pretty fibrils[J]. Sci. 2009;326(5957):1216\u20139.","journal-title":"Sci"},{"key":"4237_CR9","doi-asserted-by":"crossref","unstructured":"Du YY, Guo JL, Wang JL, Mikos AG, Zhang SM. Hierarchically designed bone scaffolds: From internal cues to external stimuli[J]. Biomater. 2019;218:11933.","DOI":"10.1016\/j.biomaterials.2019.119334"},{"issue":"14","key":"4237_CR10","doi-asserted-by":"publisher","first-page":"6695","DOI":"10.1111\/jcmm.16672","volume":"25","author":"Q Gao","year":"2021","unstructured":"Gao Q, Hou YY, Li Z, Hu JY, Huo DW, Zheng HM, Zhang JJ, Yao XY, Gao R, Wu XD, Sui L. mTORC2 regulates hierarchical micro\/nano topography-induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization[J]. J Cell Mol Med. 2021;25(14):6695\u2013708.","journal-title":"J Cell Mol Med"},{"key":"4237_CR11","first-page":"379","volume":"17","author":"R Zhao","year":"2022","unstructured":"Zhao R, Shang TL, Yuan B, Zhu XD, Zhang XD, Yang X. Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles[J]. Bioact Mater. 2022;17:379\u201393.","journal-title":"Bioact Mater"},{"key":"4237_CR12","doi-asserted-by":"crossref","unstructured":"Cui JC, Dean D, Hornicek FJ, Chen ZW, Duan ZF. The role of extracelluar matrix in osteosarcoma progression and metastasis[J]. J Exp Clin Cancer Res. 2020;39(1):178.","DOI":"10.1186\/s13046-020-01685-w"},{"issue":"5","key":"4237_CR13","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/s007760050055","volume":"3","author":"U Kk","year":"1998","unstructured":"Kk U. Osteosarcoma of bone[J]. J Orthop Sci. 1998;3(5):287\u201394.","journal-title":"J Orthop Sci"},{"key":"4237_CR14","doi-asserted-by":"crossref","unstructured":"Mondal S, Park S, Choi J, Vu TTH, Doan VHM, Vo TT, Lee B, Oh J. Hydroxyapatite: A journey from biomaterials to advanced functional materials[J]. Adv Colloid Interface Sci. 2023;321:103013.","DOI":"10.1016\/j.cis.2023.103013"},{"issue":"3","key":"4237_CR15","doi-asserted-by":"publisher","first-page":"3064","DOI":"10.1021\/acsami.3c13273","volume":"16","author":"SQ Ma","year":"2024","unstructured":"Ma SQ, Ma BB, Yang YL, Mu YZ, Wei PF, Yu XQ, Zhao B, Zou ZY, Liu ZH, Wang MG, Deng JY. Functionalized 3D Hydroxyapatite Scaffold by Fusion Peptides-Mediated Small Extracellular Vesicles of Stem Cells for Bone Tissue Regeneration[J]. ACS Appl Mater Interfaces. 2024;16(3):3064\u201381.","journal-title":"ACS Appl Mater Interfaces"},{"key":"4237_CR16","doi-asserted-by":"crossref","unstructured":"Alkaron W, Almansoori A, Bal\u00e1zsi C, Bal\u00e1zsi K. A Critical Review of Natural and Synthetic Polymer-Based Biological Apatite Composites for Bone Tissue Engineering[J]. J Compos Sci. 2024;8(12):523.","DOI":"10.3390\/jcs8120523"},{"issue":"8","key":"4237_CR17","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1038\/s41592-018-0070-7","volume":"15","author":"S Bian","year":"2018","unstructured":"Bian S, Repic M, Guo ZM, Kavirayani A, Burkard T, Bagley JA, Krauditsch C, Knoblich JA. Genetically engineered cerebral organoids model brain tumor formation[J]. Nat Methods. 2018;15(8):631\u2013.","journal-title":"Nat Methods"},{"key":"4237_CR18","doi-asserted-by":"crossref","unstructured":"Jiang TM, Xu GJ, Chen XM, Huang XY, Zhao JM, Zheng L. Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts[J]. Adv Healthc Mater. 2019;8(9):1801587.","DOI":"10.1002\/adhm.201801587"},{"key":"4237_CR19","doi-asserted-by":"crossref","unstructured":"Lu CX, Jin AX, Gao C, Qiao H, Liu HZ, Zhang Y, Sun WB, Yang SM, Liu YY. Synergistic Approach of High-Precision 3D Printing and Low Cell Adhesion for Enhanced Self-Assembled Spheroid Formation[J]. Volume 15. Biosens.-Basel; 2025. 1.","DOI":"10.3390\/bios15010007"},{"key":"4237_CR20","doi-asserted-by":"crossref","unstructured":"Jubelin C, Mu\u00f1oz-Garcia J, Cochonneau D, Ollivier E, Vallette F, Heymann MF, Oliver L, Heymann D. Technical report: liquid overlay technique allows the generation of homogeneous osteosarcoma, glioblastoma, lung and prostate adenocarcinoma spheroids that can be used for drug cytotoxicity measurements[J]. Front. Bioeng. Biotechnol., 2023, 11.","DOI":"10.3389\/fbioe.2023.1260049"},{"issue":"5","key":"4237_CR21","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1089\/ten.teb.2020.0254","volume":"27","author":"T Chow","year":"2021","unstructured":"Chow T, Wutami I, Lucarelli E, Choong PF, Duchi S, Di Bella C. Creating In Vitro Three-Dimensional Tumor Models: A Guide for the Biofabrication of a Primary Osteosarcoma Model[J]. Tissue Eng Part B-Reviews. 2021;27(5):514\u201329.","journal-title":"Tissue Eng Part B-Reviews"},{"issue":"7","key":"4237_CR22","doi-asserted-by":"publisher","first-page":"2264","DOI":"10.1016\/j.biomaterials.2013.11.038","volume":"35","author":"KM Charoen","year":"2014","unstructured":"Charoen KM, Fallica B, Colson YL, Zaman MH, Grinstaff MW. Embedded multicellular spheroids as a biomimetic 3D cancer model for evaluating drug and drug-device combinations[J]. Biomaterials. 2014;35(7):2264\u201371.","journal-title":"Biomaterials"},{"key":"4237_CR23","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.actbio.2025.08.046","volume":"205","author":"CE Monette","year":"2025","unstructured":"Monette CE, Lee J, Peasah A, Sayles LC, Tai M, Sweet-Cordero EA, Yang F. Impact of scaffold material choice on osteosarcoma phenotype and drug responses in 3D[J]. Acta Biomater. 2025;205:164\u201375.","journal-title":"Acta Biomater"},{"key":"4237_CR24","doi-asserted-by":"crossref","unstructured":"D\u00edaz ECG, Lee AG, Sayles LC, Feria C, Sweet-Cordero EA, Yang F. A 3D Osteosarcoma Model with Bone-Mimicking Cues Reveals a Critical Role of Bone Mineral and Informs Drug Discovery[J]. Adv Healthc Mater. 2022;11(17):2200768.","DOI":"10.1002\/adhm.202200768"},{"issue":"1","key":"4237_CR25","doi-asserted-by":"publisher","first-page":"40","DOI":"10.2144\/btn-2018-0083","volume":"66","author":"C Kw Jr","year":"2019","unstructured":"Kw C Jr, Jy Y, Ac C. Recent advances in photo-crosslinkable hydrogels for biomedical applications[J]. Biotechniques. 2019;66(1):40\u201353.","journal-title":"Biotechniques"},{"key":"4237_CR26","doi-asserted-by":"crossref","unstructured":"Wang ML, Xu NY, Tang RZ, Liu XQ. A 3D-printed scaffold-based osteosarcoma model allows to investigate tumor phenotypes and pathogenesis in an in vitro bone-mimicking niche[J]. Mater Today Bio. 2022;15:100295.","DOI":"10.1016\/j.mtbio.2022.100295"},{"issue":"9","key":"4237_CR27","doi-asserted-by":"publisher","first-page":"11275","DOI":"10.1021\/acsami.3c17863","volume":"16","author":"NY Xu","year":"2024","unstructured":"Xu NY, Li J, Wang ML, Chen XY, Tang RZ, Liu XQ. Fabrication of a Coculture Organoid Model in the Biomimetic Matrix of Alginate to Investigate Breast Cancer Progression in a TAMs-Leading Immune Microenvironment[J]. ACS Appl Mater Interfaces. 2024;16(9):11275\u201388.","journal-title":"ACS Appl Mater Interfaces"},{"key":"4237_CR28","doi-asserted-by":"crossref","unstructured":"Liu MX, Dai LB, Shi HZ, Xiong S, Zhou CR. In vitro evaluation of alginate\/halloysite nanotube composite scaffolds for tissue engineering[J]. Volume 49. Materials Science & Engineering C-Materials for Biological Applications; 2015. pp. 700\u201312.","DOI":"10.1016\/j.msec.2015.01.037"},{"issue":"7","key":"4237_CR29","doi-asserted-by":"publisher","first-page":"750","DOI":"10.15541\/jim20220580","volume":"38","author":"R Zhao","year":"2023","unstructured":"Zhao R, Mao F, Qian H, Yang X, Zhu XD, Zhang XD. Micro-\/Nano-structured Biomaterials for Bone Regeneration: New Progress[J]. J Inorg Mater. 2023;38(7):750\u201362.","journal-title":"J Inorg Mater"},{"key":"4237_CR30","doi-asserted-by":"crossref","unstructured":"Lian QJ, Liu H, Li JY, Luo C, Liu C, Zhao HN, Dai PJ, Wang BX, Zhou HP, Jiang X, Wang ZW, Qiao SC. Enhancing radiosensitivity of osteosarcoma by ITGB3 knockdown: a mechanism linked to enhanced osteogenic differentiation status through JNK\/c-JUN\/RUNX2 pathway activation[J]. J Exp Clin Cancer Res. 2025;44(1):159.","DOI":"10.1186\/s13046-025-03417-4"},{"key":"4237_CR31","doi-asserted-by":"crossref","unstructured":"Xiang W, Zhang BY, Lv FL, Feng GD, Chen L, Yang F, Zhang K, Cao CY, Wang PQ, Chu MX. The potential regulatory mechanisms of the gonadotropin-releasing hormone in gonadotropin transcriptions identified with bioinformatics analyses[J]. Reprod Biol Endocrinol. 2017;15:46.","DOI":"10.1186\/s12958-017-0264-3"},{"issue":"5","key":"4237_CR32","first-page":"1644","volume":"46","author":"L Chen","year":"2020","unstructured":"Chen L, Xiao DQ, Tang FJ, Gao H, Li XH. CAPN6 in disease: An emerging therapeutic target (Review)[J]. Int J Mol Med. 2020;46(5):1644\u201352.","journal-title":"Int J Mol Med"},{"key":"4237_CR33","doi-asserted-by":"crossref","unstructured":"Ji ZY, Shen JL, Lan YJ, Yi Q, Liu H. Targeting signaling pathways in osteosarcoma: Mechanisms and clinical studies[J]. Medcomm, 2023, 4(4): e308.","DOI":"10.1002\/mco2.308"},{"key":"4237_CR34","doi-asserted-by":"crossref","unstructured":"Chen YF, Chen YT, Chiu WT, Shen MR. Remodeling of calcium signaling in tumor progression[J]. J Biomed Sci. 2013;20:23.","DOI":"10.1186\/1423-0127-20-23"},{"key":"4237_CR35","doi-asserted-by":"crossref","unstructured":"Zhou DW, Fern\u00e1ndez-Yag\u00fce MA, Holland EN, Garc\u00eda AF, Castro NS, O\u2019neill EB, Eyckmans J, Chen CS, Fu JP, Schlaepfer DD, Garc\u00eda AJ. Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair[J]. Nat Commun. 2021;12(1):2359.","DOI":"10.1038\/s41467-021-22602-5"},{"issue":"1","key":"4237_CR36","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.apsb.2016.11.001","volume":"7","author":"CC Cui","year":"2017","unstructured":"Cui CC, Merritt R, Fu LW, Pan Z. Targeting calcium signaling in cancer therapy[J]. Acta Pharm Sinica B. 2017;7(1):3\u201317.","journal-title":"Acta Pharm Sinica B"},{"issue":"6","key":"4237_CR37","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nrc.2017.18","volume":"17","author":"GR Monteith","year":"2017","unstructured":"Monteith GR, Prevarskaya N, Roberts-Thomson SJ. The calcium-cancer signalling nexus[J]. Nat Rev Cancer. 2017;17(6):367\u201380.","journal-title":"Nat Rev Cancer"},{"issue":"4","key":"4237_CR38","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1007\/BF00310384","volume":"88","author":"N Ohara","year":"1994","unstructured":"Ohara N, Hayashi K, Shinohara C, Kamitani M, Furuta T, Yoshino T, Takahashi K, Taguchi K, Akagi T, PRIMARY OSTEOSARCOMA OF THE CEREBRUM WITH IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES. - REPORT OF A CASE[J]. Acta Neuropathol. 1994;88(4):384\u20138.","journal-title":"Acta Neuropathol"},{"key":"4237_CR39","doi-asserted-by":"crossref","unstructured":"Zeng M, Zhou J, Wen LF, Zhu YS, Luo YQ, Wang WC. The relationship between the expression of Ki-67 and the prognosis of osteosarcoma[J]. BMC Cancer. 2021;21(1):210.","DOI":"10.1186\/s12885-021-07880-y"},{"key":"4237_CR40","doi-asserted-by":"crossref","unstructured":"Du MR, Gu JT, Liu CL, Liu NN, Yu Z, Zhou CP, Heng W, Cao ZC, Wei FL, Zhu KL, Wang YW, Zhang W, Xue XC, Zhang Y, Qian JX. Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma[J]. J Exp Clin Cancer Res. 2022; 41(1):154.","DOI":"10.1186\/s13046-022-02344-y"},{"key":"4237_CR41","doi-asserted-by":"crossref","unstructured":"Wang B, Zhan Y, Yan L, Hao DJ. How zoledronic acid improves osteoporosis by acting on osteoclasts[J]. Front Pharmacol. 2022;13:961941.","DOI":"10.3389\/fphar.2022.961941"},{"issue":"12","key":"4237_CR42","doi-asserted-by":"publisher","first-page":"3593","DOI":"10.1158\/0008-5472.CAN-16-0061","volume":"76","author":"L Astudillo","year":"2016","unstructured":"Astudillo L, Da Silva TG, Wang ZQ, Han XQ, Jin K, Vanwye J, Zhu XX, Weaver K, Oashi T, Lopes PEM, Orton D, Neitzel LR, Lee E, Landgraf R, Robbins DJ, Mackerell AD, Capobianco AJ. The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis[J]. Cancer Res. 2016;76(12):3593\u2013603.","journal-title":"Cancer Res"},{"issue":"20","key":"4237_CR43","doi-asserted-by":"publisher","first-page":"5031","DOI":"10.1242\/dev.00697","volume":"130","author":"HT Cheng","year":"2003","unstructured":"Cheng HT, Miner JH, Lin MH, Tansey MG, Roth K, Kopan R. \u03b3-Secretase activity is dispensable for mesenchyme-to-epithelium transition but required for podocyte and proximal tubule formation in developing mouse kidney[J]. Development. 2003;130(20):5031\u201342.","journal-title":"Development"},{"issue":"23","key":"4237_CR44","doi-asserted-by":"publisher","first-page":"10712","DOI":"10.7150\/thno.46143","volume":"10","author":"WH Yan","year":"2020","unstructured":"Yan WH, Li TH, Yin TY, Hou ZJ, Qu K, Wang N, Durkan C, Dong LQ, Qiu JH, Gregersen H, Wang GX. M2 macrophage-derived exosomes promote the c-KIT phenotype of vascular smooth muscle cells during vascular tissue repair after intravascular stent implantation[J]. Theranostics. 2020;10(23):10712\u201328.","journal-title":"Theranostics"},{"key":"4237_CR45","doi-asserted-by":"crossref","unstructured":"Maloney SM, Hoover CA, Morejon-Lasso LV, Prosperi JR. Mechanisms of Taxane Resistance[J] Cancers. 2020;12(11):3323.","DOI":"10.3390\/cancers12113323"},{"key":"4237_CR46","doi-asserted-by":"crossref","unstructured":"Das T, Anand U, Pandey SK, Ashby CR, Assaraf YG, Chen ZS, Dey A. Therapeutic strategies to overcome taxane resistance in cancer[J]. Drug Resist Updates. 2021;55:100754.","DOI":"10.1016\/j.drup.2021.100754"},{"issue":"4","key":"4237_CR47","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1634\/theoncologist.2007-0220","volume":"13","author":"DR Lucas","year":"2008","unstructured":"Lucas DR, Kshirsagar MP, Biermann JS, Hamre MR, Thomas DG, Schuetze SM, Baker LH. Histologic alterations from neoadjuvant chemotherapy in high-grade extremity soft tissue sarcoma: Clinicopathological correlation[J]. Oncologist. 2008;13(4):451\u20138.","journal-title":"Oncologist"},{"key":"4237_CR48","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.cca.2014.12.041","volume":"444","author":"J Zhang","year":"2015","unstructured":"Zhang J, Yu XH, Yan YG, Wang C, Wang WJ. PI3K\/Akt signaling in osteosarcoma[J]. Clin Chim Acta. 2015;444:182\u201392.","journal-title":"Clin Chim Acta"},{"key":"4237_CR49","doi-asserted-by":"crossref","unstructured":"Assi A, Farhat M, Hachem MCR, Zalaquett Z, Aoun M, Daher M, Sebaaly A, Kourie HR. Tyrosine kinase inhibitors in osteosarcoma: Adapting treatment strategiesa[J]. J Bone Oncol. 2023;43:100511.","DOI":"10.1016\/j.jbo.2023.100511"},{"issue":"21","key":"4237_CR50","doi-asserted-by":"publisher","first-page":"5233","DOI":"10.1158\/1078-0432.CCR-17-3766","volume":"24","author":"Y Chi","year":"2018","unstructured":"Chi Y, Fang ZW, Hong XN, Yao Y, Sun P, Wang GW, Du F, Sun YK, Wu Q, Qu GF, Wang SS, Song JM, Yu JC, Lu YK, Zhu X, Niu XH, He ZY, Wang JW, Yu H, Cai JQ. Safety and Efficacy of Anlotinib, a Multikinase Angiogenesis Inhibitor, in Patients with Refractory Metastatic Soft-Tissue Sarcoma[J]. Clin Cancer Res. 2018;24(21):5233\u20138.","journal-title":"Clin Cancer Res"},{"issue":"20","key":"4237_CR51","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1056\/NEJMoa1813904","volume":"380","author":"F Andr\u00e9","year":"2019","unstructured":"Andr\u00e9 F, Ciruelos E, Rubovszky G, Campone M, Loibl S, Rugo HS, Iwata H, Conte P, Mayer IA, Kaufman B, Yamashita T, Lu YS, Inoue K, Takahashi M, P\u00e1pai Z, Longin AS, Mills D, Wilke C, Hirawat S, Juric D, Grp S-S. Alpelisib for PIK3CA-Mutated, Hormone Receptor-Positive Advanced Breast Cancer[J]. N Engl J Med. 2019;380(20):1929\u201340.","journal-title":"N Engl J Med"},{"issue":"6","key":"4237_CR52","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1093\/jjco\/hyy053","volume":"48","author":"S Miyamoto","year":"2018","unstructured":"Miyamoto S, Kakutani S, Sato Y, Hanashi A, Kinoshita Y, Ishikawa A. Drug review: Pazopanib[J]. Jpn J Clin Oncol. 2018;48(6):503\u201313.","journal-title":"Jpn J Clin Oncol"},{"key":"4237_CR53","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.actbio.2021.07.009","volume":"131","author":"C Liu","year":"2021","unstructured":"Liu C, Li M, Dong ZX, Jiang D, Li XJ, Lin SB, Chen DM, Zou XN, Zhang XD, Luker GD. Heterogeneous microenvironmental stiffness regulates pro-metastatic functions of breast cancer cells[J]. Acta Biomater. 2021;131:326\u201340.","journal-title":"Acta Biomater"},{"key":"4237_CR54","first-page":"550","volume":"49","author":"F Perin","year":"2025","unstructured":"Perin F, Ricci A, Fagiolino S, Rak-Raszewska A, Kearney H, Ramis J, Bereen I, Baker M, Maniglio D, Motta A, Moroni L, Mota C. Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidney in vitro modeling[J]. Bioact Mater. 2025;49:550\u201363.","journal-title":"Bioact Mater"},{"key":"4237_CR55","doi-asserted-by":"crossref","unstructured":"Ma XM, Yue Q, Fu S, Liu CJ, Luan J. Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers[J]. Mater Today Bio. 2024;28:101235.","DOI":"10.1016\/j.mtbio.2024.101235"},{"key":"4237_CR56","doi-asserted-by":"publisher","unstructured":"Zhang M, Feng QC, Zhang GN, Wang RH, Zhang H, Li MZ, Zhou YM, Jiang C, Li J, Nie Y. Calcium-Capturing Hydrogel with Self-Reinforced Multi-Dynamic Networks for Effective Periodontal Bone Regeneration in Three-Dimension[J]. Adv Funct Mater, 2025, Early Access, https:\/\/doi.org\/10.1002\/adfm.202415185.","DOI":"10.1002\/adfm.202415185"},{"issue":"27","key":"4237_CR57","doi-asserted-by":"publisher","first-page":"5474","DOI":"10.1016\/j.biomaterials.2005.02.002","volume":"26","author":"V Karageorgiou","year":"2005","unstructured":"Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis[J]. Biomater. 2005;26(27):5474\u201391.","journal-title":"Biomater"},{"key":"4237_CR58","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.actbio.2015.11.055","volume":"31","author":"JT Zhang","year":"2016","unstructured":"Zhang JT, Liu WZ, Gauthier O, Sourice S, Pilet P, Rethore G, Khairoun K, Bouler JM, Tancret F, Weiss P. A simple and effective approach to prepare injectable macroporous calcium phosphate cement for bone repair: Syringe-foaming using a viscous hydrophilic polymeric solution[J]. Acta Biomater. 2016;31:326\u201338.","journal-title":"Acta Biomater"},{"key":"4237_CR59","doi-asserted-by":"crossref","unstructured":"Shaabani A, Sedghi R, Motasadizadeh H, Dinarvand R. Self-healable conductive polyurethane with the body temperature-responsive shape memory for bone tissue engineering[J]. Chem Eng J. 2021;411:128449.","DOI":"10.1016\/j.cej.2021.128449"},{"key":"4237_CR60","doi-asserted-by":"crossref","unstructured":"Previdi A, Borghi F, Profumo F, Schulte C, Piazzoni C, Lamanna J, Racchetti G, Malgaroli A, Milani P. Nanotopography and Microconfinement Impact on Primary Hippocampal Astrocyte Morphology, Cytoskeleton and Spontaneous Calcium Wave Signalling[J]. Cells. 2023;12(2):293.","DOI":"10.3390\/cells12020293"},{"issue":"5","key":"4237_CR61","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1002\/cac2.12416","volume":"43","author":"A Goenka","year":"2023","unstructured":"Goenka A, Khan F, Verma B, Sinha P, Dmello CC, Jogalekar MP, Gangadaran P, Ahn BC. Tumor microenvironment signaling and therapeutics in cancer progression[J]. Cancer Commun. 2023;43(5):525\u201361.","journal-title":"Cancer Commun"},{"issue":"10","key":"4237_CR62","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1038\/s41556-023-01238-1","volume":"25","author":"W Zhang","year":"2023","unstructured":"Zhang W, Lu CH, Nakamoto ML, Tsai CT, Roy AR, Lee CE, Yang Y, Jahed Z, Li X, Cui BX. Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions[J]. Nat Cell Biol. 2023;25(10):1453\u2013.","journal-title":"Nat Cell Biol"},{"key":"4237_CR63","doi-asserted-by":"crossref","unstructured":"Lam VKL, Wong JYH, Chew SY, Chan BP. Rac1-GTPase regulates compression-induced actin protrusions (CAPs) of mesenchymal stem cells in 3D collagen micro-tissues[J]. Biomater. 2021;274:120829.","DOI":"10.1016\/j.biomaterials.2021.120829"},{"issue":"7323","key":"4237_CR64","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/nature09621","volume":"468","author":"P Kanchanawong","year":"2010","unstructured":"Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM. Nanoscale architecture of integrin-based cell adhesions[J]. Nature. 2010;468(7323):580\u2013U262.","journal-title":"Nature"},{"key":"4237_CR65","doi-asserted-by":"crossref","unstructured":"Yuan X, Zhu W, Yang ZY, He N, Chen F, Han XX, Zhou K. Recent Advances in 3D Printing of Smart Scaffolds for Bone Tissue Engineering and Regeneration[J]. Adv Mater. 2024:36(34).","DOI":"10.1002\/adma.202403641"},{"issue":"1","key":"4237_CR66","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1158\/1078-0432.CCR-23-1270","volume":"30","author":"S Singh","year":"2024","unstructured":"Singh S, Bradford D, Li XX, Mishra-Kalyani PS, Shen YL, Wang LS, Zhao H, Xiong Y, Liu J, Charlab R, Kraft J, Khasar S, Miller CP, Rivera DR, Kluetz PG, Pazdur R, Beaver JA, Singh H, Donoghue M. FDA Approval Summary: Alpelisib for PIK3CA-Related Overgrowth Spectrum[J]. Clin Cancer Res. 2024;30(1):23\u20138.","journal-title":"Clin Cancer Res"},{"key":"4237_CR67","doi-asserted-by":"crossref","unstructured":"Id SLKLLQ. O. Cancer stem cells in brain tumors: From origin to clinical implications[J]. MedComm (2020), 2023, 4(4): e341.","DOI":"10.1002\/mco2.341"},{"key":"4237_CR68","doi-asserted-by":"crossref","unstructured":"Khan SU, Fatima K, Aisha S, Malik F. Unveiling the mechanisms and challenges of cancer drug resistance[J]. Cell Commun Signal. 2024;22(1):109.","DOI":"10.1186\/s12964-023-01302-1"},{"issue":"6","key":"4237_CR69","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1634\/theoncologist.2014-0465","volume":"20","author":"YJ Zhao","year":"2015","unstructured":"Zhao YJ, Adjei AA. Targeting Angiogenesis in Cancer Therapy: Moving Beyond Vascular Endothelial Growth Factor[J]. Oncologist. 2015;20(6):660\u201373.","journal-title":"Oncologist"}],"container-title":["Journal of Nanobiotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12951-026-04237-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12951-026-04237-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12951-026-04237-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T12:40:46Z","timestamp":1776084046000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12951-026-04237-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,6]]},"references-count":69,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4237"],"URL":"https:\/\/doi.org\/10.1186\/s12951-026-04237-0","relation":{},"ISSN":["1477-3155"],"issn-type":[{"value":"1477-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,6]]},"assertion":[{"value":"3 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal study protocol was approved by the Animal Care and Use Committee (ACUC) of Experimental Animal EthicsHuazhong University of Science and Technology, protocol number 3846. The study adhered to the guidelines set by the committee.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval, consent to participate and consent to publication."}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"345"}}