{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:32:08Z","timestamp":1792873928011,"version":"4.5.0"},"reference-count":96,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T00:00:00Z","timestamp":1775347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":50,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100002858","name":"the China Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2025M773034"],"award-info":[{"award-number":["2025M773034"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005046","name":"the Heilongjiang Provincial Natural Science Foundation of China","doi-asserted-by":"crossref","award":["LH2023C023"],"award-info":[{"award-number":["LH2023C023"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Basic Research Center, Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-BRC-LPDC-2025-02"],"award-info":[{"award-number":["CAAS-BRC-LPDC-2025-02"]}]},{"name":"the Foundation of the National Research Center of Engineering and Technology for Veterinary Biologicals","award":["GTKF(23)008"],"award-info":[{"award-number":["GTKF(23)008"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1800300"],"award-info":[{"award-number":["2022YFD1800300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["ZD2023C005"],"award-info":[{"award-number":["ZD2023C005"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key Research & Development Foundation of Heilongjiang Province","award":["JD22A023"],"award-info":[{"award-number":["JD22A023"]}]},{"name":"the Innovation Program of the Chinese Academy of Agricultural Sciences","award":["CAAS-CSLPDCP-202301"],"award-info":[{"award-number":["CAAS-CSLPDCP-202301"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Nanobiotechnol"],"DOI":"10.1186\/s12951-026-04348-8","type":"journal-article","created":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T04:38:02Z","timestamp":1775363882000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nanobody-based bioPROTAC for viral protein degradation provides an antiviral strategy for porcine arterivirus"],"prefix":"10.1186","volume":"24","author":[{"given":"Shibo","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxia","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Dong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqi","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanrong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehui","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fandan","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongqing","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,5]]},"reference":[{"issue":"5","key":"4348_CR1","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1038\/s12276-021-00608-9","volume":"53","author":"YK Choi","year":"2021","unstructured":"Choi YK. Emerging and re-emerging fatal viral diseases. Exp Mol Med. 2021;53(5):711\u20132.","journal-title":"Exp Mol Med"},{"key":"4348_CR2","doi-asserted-by":"publisher","first-page":"110042","DOI":"10.1016\/j.virol.2024.110042","volume":"594","author":"S Li","year":"2024","unstructured":"Li S, Li H, Lian R, Xie J, Feng R. New perspective of small-molecule antiviral drugs development for RNA viruses. Virology. 2024;594:110042.","journal-title":"Virology"},{"key":"4348_CR3","doi-asserted-by":"publisher","first-page":"116069","DOI":"10.1016\/j.ejmech.2023.116069","volume":"265","author":"Y He","year":"2024","unstructured":"He Y, Zhou J, Gao H, Liu C, Zhan P, Liu X. Broad-spectrum antiviral strategy: Host-targeting antivirals against emerging and re-emerging viruses. Eur J Med Chem. 2024;265:116069.","journal-title":"Eur J Med Chem"},{"issue":"36","key":"4348_CR4","doi-asserted-by":"publisher","first-page":"15448","DOI":"10.1002\/anie.202004310","volume":"59","author":"LM Luh","year":"2020","unstructured":"Luh LM, Scheib U, Juenemann K, Wortmann L, Brands M, Cromm PM. Prey for the Proteasome: Targeted Protein Degradation-A Medicinal Chemist\u2019s Perspective. Angew Chem Int Edit. 2020;59(36):15448\u201366.","journal-title":"Angew Chem Int Edit"},{"issue":"13","key":"4348_CR5","doi-asserted-by":"publisher","first-page":"5330","DOI":"10.1039\/D1CS00762A","volume":"51","author":"Y Chen","year":"2022","unstructured":"Chen Y, Tandon I, Heelan W, Wang Y, Tang W, Hu Q. Proteolysis-targeting chimera (PROTAC) delivery system: advancing protein degraders towards clinical translation. Chem Soc Rev. 2022;51(13):5330\u201350.","journal-title":"Chem Soc Rev"},{"issue":"1","key":"4348_CR6","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1038\/s41392-022-00966-4","volume":"7","author":"L Zhao","year":"2022","unstructured":"Zhao L, Zhao J, Zhong K, Tong A, Jia D. Targeted protein degradation: mechanisms, strategies and application. Signal Transduct Target Ther. 2022;7(1):113.","journal-title":"Signal Transduct Target Ther"},{"issue":"7","key":"4348_CR7","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.tips.2020.04.005","volume":"41","author":"Y Ding","year":"2020","unstructured":"Ding Y, Fei Y, Lu B. Emerging New Concepts of Degrader Technologies. Trends Pharmacol Sci. 2020;41(7):464\u201374.","journal-title":"Trends Pharmacol Sci"},{"issue":"45","key":"4348_CR8","first-page":"24785","volume":"145","author":"H He","year":"2023","unstructured":"He H, Zhou C, Chen X. ATNC: Versatile Nanobody Chimeras for Autophagic Degradation of Intracellular Unligandable and Undruggable Proteins. J Am Chem Soc. 2023;145(45):24785\u201395.","journal-title":"J Am Chem Soc"},{"issue":"2","key":"4348_CR9","doi-asserted-by":"publisher","first-page":"e202100270","DOI":"10.1002\/cbic.202100270","volume":"23","author":"Z Hu","year":"2022","unstructured":"Hu Z, Crews CM. Recent Developments in PROTAC-Mediated Protein Degradation: From Bench to Clinic. ChemBioChem. 2022;23(2):e202100270.","journal-title":"ChemBioChem"},{"issue":"19","key":"4348_CR10","doi-asserted-by":"publisher","first-page":"6515","DOI":"10.3390\/molecules27196515","volume":"27","author":"J Lee","year":"2022","unstructured":"Lee J, Lee Y, Jung YM, Park JH, Yoo HS, Park J. Discovery of E3 Ligase Ligands for Target Protein Degradation. Molecules. 2022; 27(19):6515.","journal-title":"Molecules"},{"issue":"11","key":"4348_CR11","doi-asserted-by":"publisher","first-page":"5791","DOI":"10.1073\/pnas.1920251117","volume":"117","author":"S Lim","year":"2020","unstructured":"Lim S, Khoo R, Peh KM, Teo J, Chang SC, Ng S, Beilhartz GL, Melnyk RA, Johannes CW, Brown CJ, et al. bioPROTACs as versatile modulators of intracellular therapeutic targets including proliferating cell nuclear antigen (PCNA). P Natl Acad Sci USA. 2020;117(11):5791\u2013800.","journal-title":"P Natl Acad Sci USA"},{"issue":"9","key":"4348_CR12","doi-asserted-by":"publisher","first-page":"107616","DOI":"10.1016\/j.jbc.2024.107616","volume":"300","author":"M Hoffman","year":"2024","unstructured":"Hoffman M, Krum D, Wittrup KD. Bivalent target-binding bioPROTACs induce potent degradation of oncogenic SHP2. J Biol Chem. 2024;300(9):107616.","journal-title":"J Biol Chem"},{"issue":"20","key":"4348_CR13","doi-asserted-by":"publisher","first-page":"3946","DOI":"10.1111\/febs.15056","volume":"286","author":"MJ Cuneo","year":"2019","unstructured":"Cuneo MJ, Mittag T. The ubiquitin ligase adaptor SPOP in cancer. Febs j. 2019;286(20):3946\u201358.","journal-title":"Febs j"},{"issue":"1","key":"4348_CR14","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/s41392-021-00678-1","volume":"6","author":"B Wang","year":"2021","unstructured":"Wang B, Zhang L, Dai T, Qin Z, Lu H, Zhang L, Zhou F. Liquid-liquid phase separation in human health and diseases. Signal Transduct Target Ther. 2021;6(1):290.","journal-title":"Signal Transduct Target Ther"},{"issue":"33","key":"4348_CR15","doi-asserted-by":"publisher","first-page":"e2302478120","DOI":"10.1073\/pnas.2302478120","volume":"120","author":"J Li","year":"2023","unstructured":"Li J, Zhu K, Gu A, Zhang Y, Huang S, Hu R, Hu W, Lei QY, Wen W. Feedback regulation of ubiquitination and phase separation of HECT E3 ligases. Proc Natl Acad Sci U S A. 2023;120(33):e2302478120.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"4348_CR16","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1021\/acsmedchemlett.3c00498","volume":"15","author":"D Grifagni","year":"2024","unstructured":"Grifagni D, Lenci E, De Santis A, Orsetti A, Barracchia CG, Tedesco F, Bellini Puglielli R, Lucarelli F, Lauriola A, Assfalg M, et al. Development of a GC-376 Based Peptidomimetic PROTAC as a Degrader of 3-Chymotrypsin-like Protease of SARS-CoV-2. ACS Med Chem Lett. 2024;15(2):250\u20137.","journal-title":"ACS Med Chem Lett"},{"issue":"8","key":"4348_CR17","doi-asserted-by":"publisher","first-page":"6495","DOI":"10.1021\/acs.jmedchem.3c02416","volume":"67","author":"YR Alugubelli","year":"2024","unstructured":"Alugubelli YR, Xiao J, Khatua K, Kumar S, Sun L, Ma Y, Ma XR, Vulupala VR, Atla S, Blankenship LR, et al. Discovery of First-in-Class PROTAC Degraders of SARS-CoV-2 Main Protease. J Med Chem. 2024;67(8):6495\u2013507.","journal-title":"J Med Chem"},{"key":"4348_CR18","doi-asserted-by":"publisher","first-page":"129880","DOI":"10.1016\/j.bmcl.2024.129880","volume":"111","author":"D Luo","year":"2024","unstructured":"Luo D, Luo R, Wang W, Deng R, Wang S, Ma X, Pu C, Liu Y, Zhang H, Yu S, et al. Discovery of L15 as a novel Vif PROTAC degrader with antiviral activity against HIV-1. Bioorg Med Chem Lett. 2024;111:129880.","journal-title":"Bioorg Med Chem Lett"},{"issue":"16","key":"4348_CR19","doi-asserted-by":"publisher","first-page":"3402","DOI":"10.3390\/molecules30163402","volume":"30","author":"J Jin","year":"2025","unstructured":"Jin J, Quan M, Cao X, Zhang Y, Xu X, Wang Z. PROTACs in Antivirals: Current Advancements and Future Perspectives. Molecules. 2025;30(16):3402.","journal-title":"Molecules"},{"issue":"12","key":"4348_CR20","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1021\/acscentsci.2c00998","volume":"8","author":"F Shen","year":"2022","unstructured":"Shen F, Zheng G, Setegne M, Tenglin K, Izadi M, Xie H, Zhai L, Orkin SH, Dassama LMK. A Cell-Permeant Nanobody-Based Degrader That Induces Fetal Hemoglobin. ACS Cent Sci. 2022;8(12):1695\u2013703.","journal-title":"ACS Cent Sci"},{"issue":"2","key":"4348_CR21","doi-asserted-by":"publisher","first-page":"e70068","DOI":"10.1002\/mco2.70068","volume":"6","author":"C Deng","year":"2025","unstructured":"Deng C, Ma J, Liu Y, Tong X, Wang L, Dong J, Shi P, Wang M, Zheng W, Ma X. Targeting intracellular cancer proteins with tumor-microenvironment-responsive bispecific nanobody-PROTACs for enhanced therapeutic efficacy. MedComm (2020). 2025;6(2):e70068.","journal-title":"MedComm (2020)"},{"issue":"1","key":"4348_CR22","doi-asserted-by":"publisher","first-page":"5506","DOI":"10.1038\/s41467-021-25777-z","volume":"12","author":"X Chen","year":"2021","unstructured":"Chen X, Gentili M, Hacohen N, Regev A. A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies. Nat Commun. 2021;12(1):5506.","journal-title":"Nat Commun"},{"key":"4348_CR23","doi-asserted-by":"crossref","unstructured":"Truong TTT, Huynh VQ, Vo NT, Nguyen HD. Studying the characteristics of nanobody CDR regions based on sequence analysis in combination with 3D structures. J Genet Eng Biotechnolol. 2022;20(1):157.","DOI":"10.1186\/s43141-022-00439-9"},{"issue":"12","key":"4348_CR24","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1021\/acscentsci.2c00998","volume":"8","author":"FF Shen","year":"2022","unstructured":"Shen FF, Zheng G, Setegne M, Tenglin K, Izadi M, Xie H, Zhai LT, Orkin SH, Dassama LMK. A Cell-Permeant Nanobody-Based Degrader That Induces Fetal Hemoglobin. Acs Cent Sci. 2022;8(12):1695\u2013703.","journal-title":"Acs Cent Sci"},{"issue":"4","key":"4348_CR25","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.ccr.2014.02.007","volume":"25","author":"G Li","year":"2014","unstructured":"Li G, Ci W, Karmakar S, Chen K, Dhar R, Fan Z, Guo Z, Zhang J, Ke Y, Wang L, et al. SPOP promotes tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer Cell. 2014;25(4):455\u201368.","journal-title":"Cancer Cell"},{"key":"4348_CR26","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.virol.2015.02.012","volume":"479","author":"MA Kappes","year":"2015","unstructured":"Kappes MA, Faaberg KS. PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity. Virology. 2015;479:475\u201386.","journal-title":"Virology"},{"key":"4348_CR27","doi-asserted-by":"crossref","unstructured":"Li Y, Zhou L, Zhang J, Ge X, Zhou R. Nsp9 and Nsp10 Contribute to the Fatal Virulence of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China . PLoS Pathog. 2014;10(8):e1004344.","DOI":"10.1371\/journal.ppat.1004344"},{"issue":"2","key":"4348_CR28","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1017\/S1466252310000034","volume":"11","author":"N Music","year":"2010","unstructured":"Music N, Gagnon CA. The role of porcine reproductive and respiratory syndrome (PRRS) virus structural and non-structural proteins in virus pathogenesis. Anim Health Res Rev. 2010;11(2):135\u201363.","journal-title":"Anim Health Res Rev"},{"issue":"1","key":"4348_CR29","doi-asserted-by":"publisher","first-page":"e0131923","DOI":"10.1128\/jvi.01319-23","volume":"98","author":"H Duan","year":"2024","unstructured":"Duan H, Chen X, Zhang Z, Zhang Z, Li Z, Wang X, Zhao J, Nan Y, Liu B, Zhang A, et al. A nanobody inhibiting porcine reproductive and respiratory syndrome virus replication via blocking self-interaction of viral nucleocapsid protein. J Virol. 2024;98(1):e0131923.","journal-title":"J Virol"},{"issue":"1","key":"4348_CR30","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s13567-020-00868-9","volume":"52","author":"L Zhang","year":"2021","unstructured":"Zhang L, Wang L, Cao S, Lv H, Huang J, Zhang G, Tabynov K, Zhao Q, Zhou EM. Nanobody Nb6 fused with porcine IgG Fc as the delivering tag to inhibit porcine reproductive and respiratory syndrome virus replication in porcine alveolar macrophages. Vet Res. 2021;52(1):25.","journal-title":"Vet Res"},{"issue":"7","key":"4348_CR31","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1007\/s10529-016-2086-3","volume":"38","author":"H Liu","year":"2016","unstructured":"Liu H, Liang C, Duan H, Zhang X, Wang X, Xiao S, Zhou EM. Intracellularly expressed nanobodies against non-structural protein 4 of porcine reproductive and respiratory syndrome virus inhibit virus replication. Biotechnol Lett. 2016;38(7):1081\u20138.","journal-title":"Biotechnol Lett"},{"issue":"1","key":"4348_CR32","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1186\/s12951-024-02662-7","volume":"22","author":"H Yang","year":"2024","unstructured":"Yang H, Sun M, Qiu H, Xu H, Deng Z, Gu H, Wang N, Du L, Shi F, Zhou J, et al. Nanobody peptide conjugate: a novel CD163 based broad neutralizing strategy against porcine reproductive and respiratory syndrome virus. J Nanobiotechnol. 2024;22(1):388.","journal-title":"J Nanobiotechnol"},{"key":"4348_CR33","doi-asserted-by":"crossref","unstructured":"Hoz\u00e1kov\u00e1 L, Vokat\u00e1 B, Ruml T, Ulbrich P. Targeting the Virus Capsid as a Tool to Fight RNA Viruses. Viruses. 2022;14(2):174.","DOI":"10.3390\/v14020174"},{"key":"4348_CR34","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.cbpa.2018.05.010","volume":"44","author":"V Poongavanam","year":"2018","unstructured":"Poongavanam V, Doak BC, Kihlberg J. Opportunities and guidelines for discovery of orally absorbed drugs in beyond rule of 5 space. Curr Opin Chem Biol. 2018;44:23\u20139.","journal-title":"Curr Opin Chem Biol"},{"issue":"13","key":"4348_CR35","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1016\/j.bmcl.2019.04.030","volume":"29","author":"SD Edmondson","year":"2019","unstructured":"Edmondson SD, Yang B, Fallan C. Proteolysis targeting chimeras (PROTACs) in \u2018beyond rule-of-five\u2019 chemical space: Recent progress and future challenges. Bioorg Med Chem Lett. 2019;29(13):1555\u201364.","journal-title":"Bioorg Med Chem Lett"},{"issue":"16","key":"4348_CR36","doi-asserted-by":"publisher","first-page":"6092","DOI":"10.1021\/ja311795d","volume":"135","author":"EF Douglass Jr","year":"2013","unstructured":"Douglass EF Jr., Miller CJ, Sparer G, Shapiro H, Spiegel DA. A comprehensive mathematical model for three-body binding equilibria. J Am Chem Soc. 2013;135(16):6092\u20139.","journal-title":"J Am Chem Soc"},{"key":"4348_CR37","doi-asserted-by":"crossref","unstructured":"Bayda S, Adeel M, Tuccinardi T, Cordani M, Rizzolio F. The History of Nanoscience and Nanotechnology: From Chemical-Physical Applications to Nanomedicine. Molecules. 2019;25(1):112.","DOI":"10.3390\/molecules25010112"},{"issue":"12","key":"4348_CR38","doi-asserted-by":"publisher","first-page":"4448","DOI":"10.1039\/D1NR06991H","volume":"14","author":"BE Ferdows","year":"2022","unstructured":"Ferdows BE, Patel DN, Chen W, Huang X, Kong N, Tao W. RNA cancer nanomedicine: nanotechnology-mediated RNA therapy. Nanoscale. 2022;14(12):4448\u201355.","journal-title":"Nanoscale"},{"key":"4348_CR39","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.addr.2020.07.002","volume":"154\u2013155","author":"S Shah","year":"2020","unstructured":"Shah S, Dhawan V, Holm R, Nagarsenker MS, Perrie Y. Liposomes: Advancements and innovation in the manufacturing process. Adv Drug Deliv Rev. 2020;154\u2013155:102\u201322.","journal-title":"Adv Drug Deliv Rev"},{"issue":"11","key":"4348_CR40","doi-asserted-by":"publisher","first-page":"5279","DOI":"10.7150\/thno.55680","volume":"11","author":"W Wang","year":"2021","unstructured":"Wang W, Zhou Q, Jiang T, Li S, Ye J, Zheng J, Wang X, Liu Y, Deng M, Ke D, et al. A novel small-molecule PROTAC selectively promotes tau clearance to improve cognitive functions in Alzheimer-like models. Theranostics. 2021;11(11):5279\u201395.","journal-title":"Theranostics"},{"key":"4348_CR41","doi-asserted-by":"publisher","first-page":"106627","DOI":"10.1016\/j.phrs.2022.106627","volume":"188","author":"QQ Zhou","year":"2023","unstructured":"Zhou QQ, Xiao HT, Yang F, Wang YD, Li P, Zheng ZG. Advancing targeted protein degradation for metabolic diseases therapy. Pharmacol Res. 2023;188:106627.","journal-title":"Pharmacol Res"},{"key":"4348_CR42","doi-asserted-by":"crossref","unstructured":"Zhang HL, Xiang LR, Xu H, Li C, Tang YD, Gong BJ, Zhang WL, Zhao J, Song SJ, Peng JM et al. Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets. Vaccines-Basel. 2022;10(5):752.","DOI":"10.3390\/vaccines10050752"},{"key":"4348_CR43","doi-asserted-by":"crossref","unstructured":"Xu H, Li C, Gong BJ, Li WS, Guo ZY, Sun Q, Zhao J, Xiang LR, Li JH, Tang YD et al. Protective Efficacy of a Candidate Live-Attenuated Vaccine Derived from the SD-R Strain against NADC34-like Porcine Reproductive and Respiratory Syndrome Virus. Vaccines-Basel. 2023;11(8):1349.","DOI":"10.3390\/vaccines11081349"},{"key":"4348_CR44","doi-asserted-by":"crossref","unstructured":"Xu H, Gong BJ, Sun Q, Li C, Zhao J, Xiang LR, Li WS, Guo ZY, Tang YD, Leng CL et al. Genomic Characterization and Pathogenicity of BJEU06-1-Like PRRSV-1 ZD-1 Isolated in China. Transboundary and Emerging Diseases. 2023;2023:6793604.","DOI":"10.1155\/2023\/6793604"},{"key":"4348_CR45","doi-asserted-by":"crossref","unstructured":"Zhang YJ, Zhang K, Zheng H, Liu CL, Jiang YF, Du NN, Li LW, Li GX, Yu LX, Zhou YJ et al. Development of a Monoclonal Antibody Against Porcine CD163 SRCR5 Domain Which Partially Blocks Infection of PRRSV. Front Veterinary Sci. 2020;7:597843.","DOI":"10.3389\/fvets.2020.597843"},{"key":"4348_CR46","doi-asserted-by":"publisher","first-page":"144248","DOI":"10.1016\/j.ijbiomac.2025.144248","volume":"312","author":"M Sun","year":"2025","unstructured":"Sun M, Cao D, Zhao M, Zhu X, Sun Y, Wang Q, Zhang H, Yang Y, Tian X, Wang H, et al. Nanobody-based competitive enzyme-linked immunosorbent assay for detecting antibodies of porcine reproductive and respiratory syndrome virus. Int J Biol Macromol. 2025;312:144248.","journal-title":"Int J Biol Macromol"},{"key":"4348_CR47","doi-asserted-by":"publisher","first-page":"139670","DOI":"10.1016\/j.ijbiomac.2025.139670","volume":"296","author":"S Wang","year":"2025","unstructured":"Wang S, Chen T, Yang YB, Wang H, Sun M, Gao K, Wei Z, Bai Y, Tang YD, Cai X, et al. Development of blocking ELISA for detection anti-PRRSV antibodies and serological investigation of PRRSV in China. Int J Biol Macromol. 2025;296:139670.","journal-title":"Int J Biol Macromol"},{"key":"4348_CR48","doi-asserted-by":"crossref","unstructured":"Chen M, Wang MH, Shen XG, Liu H, Zhang YY, Peng JM, Meng F, Wang TY, Bai YZ, Sun MX et al. Neuropilin-1 Facilitates Pseudorabies Virus Replication and Viral Glycoprotein B Promotes Its Degradation in a Furin-Dependent Manner. J Virol. 2022;96(20):e0131822.","DOI":"10.1128\/jvi.01318-22"},{"key":"4348_CR49","doi-asserted-by":"publisher","first-page":"1387309","DOI":"10.3389\/fmicb.2024.1387309","volume":"15","author":"H Zhou","year":"2024","unstructured":"Zhou H, Sun M, Su S, Meng L, Yang W, Yang L, Shi X, Li X, Wang H, Ma H, et al. Identification of a linear B-cell epitope on the puff loop of the Senecavirus A VP2 protein involved in receptor binding. Front Microbiol. 2024;15:1387309.","journal-title":"Front Microbiol"},{"key":"4348_CR50","doi-asserted-by":"crossref","unstructured":"Yu F, Liu LQ, Tian XX, Chen LG, Huang XY, Sun Y, Yan Y, Tian ZJ, Cai XH, Liu D et al. Genomic Analysis of Porcine Reproductive and Respiratory Syndrome Virus 1 Revealed Extensive Recombination and Potential Introduction Events in China. Veterinary Sci.2022;9(9):450.","DOI":"10.3390\/vetsci9090450"},{"issue":"6","key":"4348_CR51","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1111\/tbed.13276","volume":"66","author":"NH Chen","year":"2019","unstructured":"Chen NH, Ye MX, Xiao YZ, Li S, Huang YC, Li XD, Tian KG, Zhu JZ. Development of universal and quadruplex real-time RT-PCR assays for simultaneous detection and differentiation of porcine reproductive and respiratory syndrome viruses. Transbound Emerg Dis. 2019;66(6):2271\u20138.","journal-title":"Transbound Emerg Dis"},{"issue":"1","key":"4348_CR52","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1099\/jgv.0.000316","volume":"97","author":"D Tian","year":"2016","unstructured":"Tian D, Meng XJ. Amino acid residues Ala283 and His421 in the RNA-dependent RNA polymerase of porcine reproductive and respiratory syndrome virus play important roles in viral ribavirin sensitivity and quasispecies diversity. J Gen Virol. 2016;97(1):53\u20139.","journal-title":"J Gen Virol"},{"key":"4348_CR53","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s12917-015-0330-z","volume":"11","author":"A Khatun","year":"2015","unstructured":"Khatun A, Shabir N, Yoon KJ, Kim WI. Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus. BMC Vet Res. 2015;11:21.","journal-title":"BMC Vet Res"},{"issue":"38","key":"4348_CR54","doi-asserted-by":"publisher","first-page":"22783","DOI":"10.1039\/D0RA04070C","volume":"10","author":"Y Chen","year":"2020","unstructured":"Chen Y, Li H, Wu L, Zhang M, Gao Y, Wang H, Xu D, Chen W, Song G, Chen J. Ursolic acid derivatives are potent inhibitors against porcine reproductive and respiratory syndrome virus. RSC Adv. 2020;10(38):22783\u201396.","journal-title":"RSC Adv"},{"issue":"9","key":"4348_CR55","doi-asserted-by":"publisher","first-page":"4103","DOI":"10.1128\/AAC.00294-11","volume":"55","author":"L Delang","year":"2011","unstructured":"Delang L, Vliegen I, Froeyen M, Neyts J. Comparative study of the genetic barriers and pathways towards resistance of selective inhibitors of hepatitis C virus replication. Antimicrob Agents Chemother. 2011;55(9):4103\u201313.","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"4348_CR56","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/s13567-021-00914-0","volume":"52","author":"Z Zhang","year":"2021","unstructured":"Zhang Z, Zhai T, Li M, Zhang K, Li J, Zheng X, Tian C, Chen R, Dong J, Zhou EM, et al. A broadly neutralizing monoclonal antibody induces broad protection against heterogeneous PRRSV strains in piglets. Vet Res. 2021;52(1):45.","journal-title":"Vet Res"},{"issue":"1","key":"4348_CR57","doi-asserted-by":"publisher","first-page":"4813","DOI":"10.1038\/s41467-024-48807-y","volume":"15","author":"Z Cui","year":"2024","unstructured":"Cui Z, Liu J, Xie C, Wang T, Sun P, Wang J, Li J, Li G, Qiu J, Zhang Y, et al. High-throughput screening unveils nitazoxanide as a potent PRRSV inhibitor by targeting NMRAL1. Nat Commun. 2024;15(1):4813.","journal-title":"Nat Commun"},{"issue":"1","key":"4348_CR58","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.molcel.2009.09.022","volume":"36","author":"M Zhuang","year":"2009","unstructured":"Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, et al. Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Mol Cell. 2009;36(1):39\u201350.","journal-title":"Mol Cell"},{"key":"4348_CR59","doi-asserted-by":"publisher","first-page":"353","DOI":"10.2147\/IJN.S387160","volume":"18","author":"Y Lu","year":"2023","unstructured":"Lu Y, Li Q, Fan H, Liao C, Zhang J, Hu H, Yi H, Peng Y, Lu J, Chen Z. A Multivalent and Thermostable Nanobody Neutralizing SARS-CoV-2 Omicron (B.1.1.529). Int J Nanomed. 2023;18:353\u201367.","journal-title":"Int J Nanomed"},{"issue":"8","key":"4348_CR60","doi-asserted-by":"publisher","first-page":"3825","DOI":"10.1128\/JVI.02687-09","volume":"84","author":"D Atanasiu","year":"2010","unstructured":"Atanasiu D, Whitbeck JC, de Leon MP, Lou H, Hannah BP, Cohen GH, Eisenberg RJ. Bimolecular Complementation Defines Functional Regions of Herpes Simplex Virus gB That Are Involved with gH\/gL as a Necessary Step Leading to Cell Fusion. J Virol. 2010;84(8):3825\u201334.","journal-title":"J Virol"},{"issue":"1","key":"4348_CR61","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.molcel.2020.04.032","volume":"79","author":"AFM Ibrahim","year":"2020","unstructured":"Ibrahim AFM, Shen L, Tatham MH, Dickerson D, Prescott AR, Abidi N, Xirodimas DP, Hay RT. Antibody RING-Mediated Destruction of Endogenous Proteins. Mol Cell. 2020;79(1):155\u2013e166159.","journal-title":"Mol Cell"},{"issue":"11","key":"4348_CR62","doi-asserted-by":"publisher","first-page":"5390","DOI":"10.1021\/acs.jproteome.5c00121","volume":"24","author":"D Kovanich","year":"2025","unstructured":"Kovanich D, Ketsuwan K, Hengphasatporn K, Thepparit C, Sittipaisankul P, Wongkongkathep P, Sirisereewan C, Techakriengkrai N, Nedumpun T, Shigeta Y, et al. Analysis of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Interactome. J Proteome Res. 2025;24(11):5390\u2013411.","journal-title":"J Proteome Res"},{"issue":"6381","key":"4348_CR63","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1126\/science.aag3048","volume":"359","author":"DS Leeman","year":"2018","unstructured":"Leeman DS, Hebestreit K, Ruetz T, Webb AE, McKay A, Pollina EA, Dulken B, Zhao XA, Yeo RW, Ho TT, et al. Lysosome activation clears aggregates and enhances quiescent neural stem cell activation during aging. Science. 2018;359(6381):1277\u201382.","journal-title":"Science"},{"key":"4348_CR64","doi-asserted-by":"crossref","unstructured":"Hansen TE, Johansen T. Following autophagy step by step. Bmc Biol. 2011;9:39.","DOI":"10.1186\/1741-7007-9-39"},{"key":"4348_CR65","doi-asserted-by":"crossref","unstructured":"Xu H, Li C, Gong B, Li W, Guo Z, Sun Q, Zhao J, Xiang L, Li J, Tang YD et al. Protective Efficacy of a Candidate Live-Attenuated Vaccine Derived from the SD-R Strain against NADC34-like Porcine Reproductive and Respiratory Syndrome Virus. Vaccines (Basel). 2023;11(8):1349.","DOI":"10.3390\/vaccines11081349"},{"key":"4348_CR66","doi-asserted-by":"crossref","unstructured":"Xu H, Gong B, Sun Q, Li C, Zhao J, Xiang L, Li W, Guo Z, Tang YD, Leng C et al. Genomic Characterization and Pathogenicity of BJEU06-1-Like PRRSV-1 ZD-1 Isolated in China. Transbound Emerg Dis 2023;2023:6793604.","DOI":"10.1155\/2023\/6793604"},{"key":"4348_CR67","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","volume":"4","author":"JK Lunney","year":"2016","unstructured":"Lunney JK, Fang Y, Ladinig A, Chen N, Li Y, Rowland B, Renukaradhya GJ. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System. Annu Rev Anim Biosci. 2016;4:129\u201354.","journal-title":"Annu Rev Anim Biosci"},{"key":"4348_CR68","doi-asserted-by":"publisher","first-page":"1460973","DOI":"10.3389\/fvets.2024.1460973","volume":"11","author":"S Su","year":"2024","unstructured":"Su S, Shen X, Shi X, Li X, Chen J, Yang W, Sun M, Tang YD, Wang H, Wang S, et al. Cell-penetrating peptides TAT and 8R functionalize P22 virus-like particles to enhance tissue distribution and retention in vivo. Front Vet Sci. 2024;11:1460973.","journal-title":"Front Vet Sci"},{"key":"4348_CR69","doi-asserted-by":"crossref","unstructured":"Liu Z, Hu MX, Yang Y, Du CH, Zhou HX, Liu CYL, Chen YW, Fan L, Ma HQ, Gong YL et al. An overview of PROTACs: a promising drug discovery paradigm. Mol Biomed. 2022;3(1):46.","DOI":"10.1186\/s43556-022-00112-0"},{"issue":"4","key":"4348_CR70","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1016\/j.chembiol.2024.02.004","volume":"31","author":"LA Emert-Sedlak","year":"2024","unstructured":"Emert-Sedlak LA, Tice CM, Shi H, Alvarado JJ, Shu ST, Reitz AB, Smithgall TE. PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication. Cell Chem Biol. 2024;31(4):658\u2013e668614.","journal-title":"Cell Chem Biol"},{"key":"4348_CR71","doi-asserted-by":"crossref","unstructured":"Song J, Liu Y, Gao P, Hu Y, Chai Y, Zhou S, Kong C, Zhou L, Ge X, Guo X et al. Mapping the Nonstructural Protein Interaction Network of Porcine Reproductive and Respiratory Syndrome Virus. J Virol. 2018;92(24):e01112-18.","DOI":"10.1128\/JVI.01112-18"},{"issue":"Pt 5","key":"4348_CR72","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1099\/0022-1317-82-5-985","volume":"82","author":"EJ Snijder","year":"2001","unstructured":"Snijder EJ, van Tol H, Roos N, Pedersen KW. Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. J Gen Virol. 2001;82(Pt 5):985\u201394.","journal-title":"J Gen Virol"},{"issue":"1\u20132","key":"4348_CR73","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.virusres.2010.07.030","volume":"154","author":"Y Fang","year":"2010","unstructured":"Fang Y, Snijder EJ. The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins. Virus Res. 2010;154(1\u20132):61\u201376.","journal-title":"Virus Res"},{"issue":"9","key":"4348_CR74","doi-asserted-by":"publisher","first-page":"4480","DOI":"10.1128\/JVI.02756-07","volume":"82","author":"CC Posthuma","year":"2008","unstructured":"Posthuma CC, Pedersen KW, Lu Z, Joosten RG, Roos N, Zevenhoven-Dobbe JC, Snijder EJ. Formation of the arterivirus replication\/transcription complex: a key role for nonstructural protein 3 in the remodeling of intracellular membranes. J Virol. 2008;82(9):4480\u201391.","journal-title":"J Virol"},{"key":"4348_CR75","doi-asserted-by":"crossref","unstructured":"Sha HY, Zhang H, Chen Y, Huang LZ, Zhao MM, Wang NA. Research Progress on the NSP9 Protein of Porcine Reproductive and Respiratory Syndrome Virus. Front Veterinary Sci. 2022;9:872205.","DOI":"10.3389\/fvets.2022.872205"},{"issue":"2","key":"4348_CR76","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.virusres.2003.12.026","volume":"100","author":"Y Fang","year":"2004","unstructured":"Fang Y, Kim DY, Ropp S, Steen P, Christopher-Hennings J, Nelson EA, Rowland RRR. Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res. 2004;100(2):229\u201335.","journal-title":"Virus Res"},{"key":"4348_CR77","doi-asserted-by":"crossref","unstructured":"Zhang YM, Sun Y, Xie YC, Shang WJ, Wang Z, Jiang HL, Shen JS, Xiao GF, Zhang LK. A viral RNA-dependent RNA polymerase inhibitor VV116 broadly inhibits human coronaviruses and has synergistic potency with 3CLpro inhibitor nirmatrelvir. Signal Transduct Tar. 2023;8(1):360.","DOI":"10.1038\/s41392-023-01587-1"},{"key":"4348_CR78","doi-asserted-by":"crossref","unstructured":"Velthuis AJWT, van den Worm SHE, Sims AC, Baric RS, Snijder EJ, van Hemert MJ. Zn2\u2009+\u2009Inhibits Coronavirus and Arterivirus RNA Polymerase Activity In Vitro and Zinc Ionophores Block the Replication of These Viruses in Cell Culture. PLoS Pathog. 2010;6(11):e1001176.","DOI":"10.1371\/journal.ppat.1001176"},{"issue":"1","key":"4348_CR79","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.molcel.2018.08.027","volume":"72","author":"JJ Bouchard","year":"2018","unstructured":"Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, et al. Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments. Mol Cell. 2018;72(1):19\u2013e3618.","journal-title":"Mol Cell"},{"issue":"5","key":"4348_CR80","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/j.molcel.2019.09.009","volume":"76","author":"D Takahashi","year":"2019","unstructured":"Takahashi D, Moriyama J, Nakamura T, Miki E, Takahashi E, Sato A, Akaike T, Itto-Nakama K, Arimoto H. AUTACs: Cargo-Specific Degraders Using Selective Autophagy. Mol Cell. 2019;76(5):797\u2013810.","journal-title":"Mol Cell"},{"issue":"1","key":"4348_CR81","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.cell.2019.11.031","volume":"181","author":"GM Burslem","year":"2020","unstructured":"Burslem GM, Crews CM. Proteolysis-Targeting Chimeras as Therapeutics and Tools for Biological Discovery. Cell. 2020;181(1):102\u201314.","journal-title":"Cell"},{"key":"4348_CR82","doi-asserted-by":"crossref","unstructured":"Steven Banik, Kayvon Pedram, Simon Wisnovsky, Nicholas Riley, Carolyn Bertozzi. Lysosome targeting Chimeras (LYTACs) for the degradation of secreted and membrane proteins. chemrxiv. 2019.","DOI":"10.26434\/chemrxiv.7927061.v1"},{"issue":"5","key":"4348_CR83","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/j.molcel.2019.09.009","volume":"76","author":"D Takahashi","year":"2019","unstructured":"Takahashi D, Moriyama J, Nakamura T, Miki E, Takahashi E, Sato A, Akaike T, Itto-Nakama K, Arimoto H. AUTACs: Cargo-Specific Degraders Using Selective Autophagy. Mol Cell. 2019;76(5):797\u2013e810710.","journal-title":"Mol Cell"},{"key":"4348_CR84","doi-asserted-by":"crossref","unstructured":"Yang G, Chen PL, Rovito SW, Minari K, Writt HN, DeBeauchamp J, Crumpton JC, Kercher L, DuBois RM, Webby RJ et al. Resistance Mutations to Broadly Neutralizing Antibodies Destabilize Hemagglutinin and Attenuate H1N1 Influenza Virus. Viruses. 2025;18(1):32.","DOI":"10.3390\/v18010032"},{"issue":"3\u20134","key":"4348_CR85","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/j.vetmic.2015.10.021","volume":"181","author":"H Liu","year":"2015","unstructured":"Liu H, Wang Y, Duan H, Zhang A, Liang C, Gao J, Zhang C, Huang B, Li Q, Li N, et al. An intracellularly expressed Nsp9-specific nanobody in MARC-145 cells inhibits porcine reproductive and respiratory syndrome virus replication. Vet Microbiol. 2015;181(3\u20134):252\u201360.","journal-title":"Vet Microbiol"},{"key":"4348_CR86","doi-asserted-by":"publisher","first-page":"1726449","DOI":"10.3389\/fimmu.2025.1726449","volume":"16","author":"CR Ball","year":"2025","unstructured":"Ball CR, Ramage W, Mate R, Hufton SE. Susceptibility of broad reactivity nanobodies to resistance mutations in the S2 domain of SARS-CoV-2 predicted by yeast display deep mutational scanning. Front Immunol. 2025;16:1726449.","journal-title":"Front Immunol"},{"issue":"1","key":"4348_CR87","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/jps.23783","volume":"103","author":"B Wu","year":"2014","unstructured":"Wu B, Sun YN. Pharmacokinetics of Peptide-Fc fusion proteins. J Pharm Sci. 2014;103(1):53\u201364.","journal-title":"J Pharm Sci"},{"key":"4348_CR88","doi-asserted-by":"crossref","unstructured":"Mu XMC, Wang M, Meng C, Liu H, Lu H. Nanotechnology Advances Proteolysis Targeting Chimeras (PROTACs): Transition From Basic Research to Clinical Application. 2025;20:12177-98.","DOI":"10.2147\/IJN.S552644"},{"issue":"1","key":"4348_CR89","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1186\/s13287-022-03180-9","volume":"13","author":"Y Sun","year":"2022","unstructured":"Sun Y, Li B, Cao Q, Liu T, Li J. Targeting cancer stem cells with polymer nanoparticles for gastrointestinal cancer treatment. Stem Cell Res Ther. 2022;13(1):489.","journal-title":"Stem Cell Res Ther"},{"key":"4348_CR90","doi-asserted-by":"publisher","first-page":"100423","DOI":"10.1016\/j.mtbio.2022.100423","volume":"16","author":"Y He","year":"2022","unstructured":"He Y, Zan X, Miao J, Wang B, Wu Y, Shen Y, Chen X, Gou H, Zheng S, Huang N, et al. Enhanced anti-glioma efficacy of doxorubicin with BRD4 PROTAC degrader using targeted nanoparticles. Mater Today Bio. 2022;16:100423.","journal-title":"Mater Today Bio"},{"issue":"15","key":"4348_CR91","doi-asserted-by":"publisher","first-page":"10458","DOI":"10.1021\/acs.jmedchem.3c00587","volume":"66","author":"Q He","year":"2023","unstructured":"He Q, Zhou L, Yu D, Zhu R, Chen Y, Song M, Liu X, Liao Y, Ding T, Fan W, et al. Near-Infrared-Activatable PROTAC Nanocages for Controllable Target Protein Degradation and On-Demand Antitumor Therapy. J Med Chem. 2023;66(15):10458\u201372.","journal-title":"J Med Chem"},{"issue":"1","key":"4348_CR92","doi-asserted-by":"publisher","first-page":"9374","DOI":"10.1038\/s41467-025-64426-7","volume":"16","author":"R Zhou","year":"2025","unstructured":"Zhou R, Wang H, Zhang GM, Liu Y, Liu XL, Li Z, Shi G, Yuan J, Qu C, Li Y, et al. Targeted degradation of endogenous YAP by nanobody bioPROTAC inhibits tumor progression. Nat Commun. 2025;16(1):9374.","journal-title":"Nat Commun"},{"issue":"1","key":"4348_CR93","doi-asserted-by":"publisher","first-page":"5808","DOI":"10.1038\/s41467-024-50235-x","volume":"15","author":"A Chan","year":"2024","unstructured":"Chan A, Haley RM, Najar MA, Gonzalez-Martinez D, Bugaj LJ, Burslem GM, Mitchell MJ, Tsourkas A. Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins. Nat Commun. 2024;15(1):5808.","journal-title":"Nat Commun"},{"key":"4348_CR94","doi-asserted-by":"publisher","first-page":"5739","DOI":"10.2147\/IJN.S448684","volume":"19","author":"Y Song","year":"2024","unstructured":"Song Y, Dong QQ, Ni YK, Xu XL, Chen CX, Chen W. Nano-Proteolysis Targeting Chimeras (Nano-PROTACs) in Cancer Therapy. Int J Nanomed. 2024;19:5739\u201361.","journal-title":"Int J Nanomed"},{"issue":"1","key":"4348_CR95","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/s12951-019-0514-y","volume":"17","author":"W Wang","year":"2019","unstructured":"Wang W, Lu KJ, Yu CH, Huang QL, Du YZ. Nano-drug delivery systems in wound treatment and skin regeneration. J Nanobiotechnol. 2019;17(1):82.","journal-title":"J Nanobiotechnol"},{"issue":"9","key":"4348_CR96","doi-asserted-by":"publisher","first-page":"4378","DOI":"10.1039\/D3NR06059D","volume":"16","author":"J Zhong","year":"2024","unstructured":"Zhong J, Zhao R, Wang Y, Su YX, Lan X. Nano-PROTACs: state of the art and perspectives. Nanoscale. 2024;16(9):4378\u201391.","journal-title":"Nanoscale"}],"container-title":["Journal of Nanobiotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12951-026-04348-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12951-026-04348-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12951-026-04348-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T01:01:55Z","timestamp":1779670915000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12951-026-04348-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,5]]},"references-count":96,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4348"],"URL":"https:\/\/doi.org\/10.1186\/s12951-026-04348-8","relation":{},"ISSN":["1477-3155"],"issn-type":[{"value":"1477-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,5]]},"assertion":[{"value":"18 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were performed within animal biosafety level 2 (ABSL-2) facilities under oversight and were approved by the Animal Ethics Committee of the Harbin Veterinary Research Institute. The animal ethics committee approval numbers were 231207-03-GR for the mouse experiments and 250220-06-GR for the piglet experiments. Consent to Participate declaration: not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Consent to Publish declaration: not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Tongqing An and Fandan Meng filed a patent related to this technology.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"454"}}