{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T16:11:21Z","timestamp":1793722281180,"version":"4.6.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,10,23]],"date-time":"2021-10-23T00:00:00Z","timestamp":1634947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,10,23]],"date-time":"2021-10-23T00:00:00Z","timestamp":1634947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Surg Onc"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The present study aims to investigate the preliminary mechanism underlying the peritoneal metastasis of gastric cancer cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Exosomes from GC9811 cells (Con-Exo) and from GC9811-P cells (PM-Exo) were extracted by ultracentrifugation, which were identified with transmission electron microscopy (TEM) and nanoparticle trafficking analysis, as well as the expression of CD9, CD63, and CD81 detected by Western blot assay.\n                      <jats:italic>\u03b1<\/jats:italic>\n                      -SMA expression was determined by immunofluorescence assay and Western blot assay. The levels of Snail1, E-cadherin, and Actin-related protein 3 (ACTR3) were evaluated by Western blot assay. MiRNA array was performed on exosomes to screen the differentially expressed miRNAs. The expressions of miRNAs,\n                      <jats:italic>SMAD2<\/jats:italic>\n                      ,\n                      <jats:italic>CDK4<\/jats:italic>\n                      , and\n                      <jats:italic>ACTR3<\/jats:italic>\n                      were determined by QRT-PCR. The delivery of miR-486-5p was confirmed by laser confocal detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Firstly, TEM, nanoparticle trafficking analysis, and Western blot assays were used to confirm the successful extraction of Con-Exo and PM-Exo. The incubation of Con-Exo and PM-Exo could decrease E-cadherin expression and increase of\n                      <jats:italic>\u03b1<\/jats:italic>\n                      -SMA respectively in HMrSV5 cells, with the increased proportion of fusiform cells. More significant changes were observed in PM-Exo-treated HMrSV5 cells. Secondary, compared to Con-Exo, miR-486-5p and miR-132-3p were found downregulated, and miR-132-5p was found upregulated in PM-Exo. The transfection of miR-486-5p and miR-132-3p was observed to suppress EMT, and the transfection of miR-132-3p was observed to induce EMT. Laser confocal detection confirmed the delivery of miR-486-5p from gastric cancer cells to HMrSV5 cells through exosomes. Lastly, the expression of Mothers against decapentaplegic homolog 2 (SMAD2), cyclin-dependent kinase 4 (CDK4), and ACTR3 was found to be downregulated via miR-486-5p.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Decreased delivery of miR-486-5p via exosomes might be responsible for the peritoneal metastasis of gastric cancer cells by promoting epithelial-mesenchymal transition progress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12957-021-02381-5","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T21:04:44Z","timestamp":1634936684000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Decreased exosome-delivered miR-486-5p is responsible for the peritoneal metastasis of gastric cancer cells by promoting EMT progress"],"prefix":"10.1186","volume":"19","author":[{"given":"Xian-Ming","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Jiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xiao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,23]]},"reference":[{"issue":"2","key":"2381_CR1","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1002\/ags3.12060","volume":"2","author":"J Kitayama","year":"2018","unstructured":"Kitayama J, Ishigami H, Yamaguchi H, Sakuma Y, Horie H, Hosoya Y, et al. Treatment of patients with peritoneal metastases from gastric cancer. Annals of gastroenterological surgery. 2018;2(2):116\u201323. https:\/\/doi.org\/10.1002\/ags3.12060.","journal-title":"Annals of gastroenterological surgery"},{"issue":"10060","key":"2381_CR2","doi-asserted-by":"publisher","first-page":"2606","DOI":"10.1016\/S0140-6736(16)32226-7","volume":"388","author":"L Zong","year":"2016","unstructured":"Zong L, Abe M, Seto Y, Ji J. The challenge of screening for early gastric cancer in China. Lancet. 2016;388(10060):2606. https:\/\/doi.org\/10.1016\/S0140-6736(16)32226-7.","journal-title":"Lancet"},{"issue":"11","key":"2381_CR3","doi-asserted-by":"publisher","first-page":"4012","DOI":"10.3390\/ijms21114012","volume":"21","author":"J Machlowska","year":"2020","unstructured":"Machlowska J, Baj J, Sitarz M, Maciejewski R, Sitarz R. Gastric cancer: epidemiology, risk factors, classification, genomic characteristics and treatment strategies. Int J Mol Sci. 2020;21(11):4012. https:\/\/doi.org\/10.3390\/ijms21114012.","journal-title":"Int J Mol Sci"},{"issue":"5","key":"2381_CR4","doi-asserted-by":"publisher","first-page":"1448","DOI":"10.1245\/s10434-013-3327-5","volume":"21","author":"D Magge","year":"2014","unstructured":"Magge D, Zenati M, Mavanur A, Winer J, Ramalingam L, Jones H, et al. Aggressive locoregional surgical therapy for gastric peritoneal carcinomatosis. Ann Surg Oncol. 2014;21(5):1448\u201355. https:\/\/doi.org\/10.1245\/s10434-013-3327-5.","journal-title":"Ann Surg Oncol"},{"issue":"3","key":"2381_CR5","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1002\/ijc.28373","volume":"134","author":"I Thomassen","year":"2014","unstructured":"Thomassen I, van Gestel YR, van Ramshorst B, Luyer MD, Bosscha K, Nienhuijs SW, et al. Peritoneal carcinomatosis of gastric origin: a population-based study on incidence, survival and risk factors. Int J Cancer. 2014;134(3):622\u20138. https:\/\/doi.org\/10.1002\/ijc.28373.","journal-title":"Int J Cancer"},{"key":"2381_CR6","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/1471-230X-12-34","volume":"12","author":"D Na","year":"2012","unstructured":"Na D, Lv ZD, Liu FN, et al. Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo. BMC Gastroenterol. 2012;12:34.","journal-title":"BMC Gastroenterol"},{"issue":"24","key":"2381_CR7","doi-asserted-by":"publisher","first-page":"3176","DOI":"10.1038\/onc.2014.246","volume":"34","author":"R Satoyoshi","year":"2015","unstructured":"Satoyoshi R, Aiba N, Yanagihara K, Yashiro M, Tanaka M. Tks5 activation in mesothelial cells creates invasion front of peritoneal carcinomatosis. Oncogene. 2015;34(24):3176\u201387. https:\/\/doi.org\/10.1038\/onc.2014.246.","journal-title":"Oncogene"},{"issue":"4","key":"2381_CR8","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1002\/cam4.2041","volume":"8","author":"S Yin","year":"2019","unstructured":"Yin S, Miao Z, Tan Y, Wang P, Xu X, Zhang C, et al. SPHK1-induced autophagy in peritoneal mesothelial cell enhances gastric cancer peritoneal dissemination. Cancer Med. 2019;8(4):1731\u201343. https:\/\/doi.org\/10.1002\/cam4.2041.","journal-title":"Cancer Med"},{"key":"2381_CR9","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.biopha.2017.01.039","volume":"88","author":"ZD Lv","year":"2017","unstructured":"Lv ZD, Zhao WJ, Jin LY, et al. Blocking TGF-beta1 by P17 peptides attenuates gastric cancer cell induced peritoneal fibrosis and prevents peritoneal dissemination in vitro and in vivo. Biomed Pharmacother. 2017;88:27\u201333.","journal-title":"Biomed Pharmacother"},{"issue":"4","key":"2381_CR10","doi-asserted-by":"publisher","first-page":"3575","DOI":"10.1007\/s13277-013-1472-x","volume":"35","author":"ZF Miao","year":"2014","unstructured":"Miao ZF, Zhao TT, Wang ZN, Miao F, Xu YY, Mao XY, et al. Transforming growth factor-beta1 signaling blockade attenuates gastric cancer cell-induced peritoneal mesothelial cell fibrosis and alleviates peritoneal dissemination both in vitro and in vivo. Tumour Biol. 2014;35(4):3575\u201383. https:\/\/doi.org\/10.1007\/s13277-013-1472-x.","journal-title":"Tumour Biol"},{"issue":"4","key":"2381_CR11","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1002\/hep.29920","volume":"68","author":"JH Fang","year":"2018","unstructured":"Fang JH, Zhang ZJ, Shang LR, Luo YW, Lin YF, Yuan Y, et al. Hepatoma cell-secreted exosomal microRNA-103 increases vascular permeability and promotes metastasis by targeting junction proteins. Hepatology. 2018;68(4):1459\u201375. https:\/\/doi.org\/10.1002\/hep.29920.","journal-title":"Hepatology"},{"issue":"1","key":"2381_CR12","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1038\/s41467-019-08989-2","volume":"10","author":"AC Frank","year":"2019","unstructured":"Frank AC, Ebersberger S, Fink AF, Lampe S, Weigert A, Schmid T, et al. Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype. Nat Commun. 2019;10(1):1135. https:\/\/doi.org\/10.1038\/s41467-019-08989-2.","journal-title":"Nat Commun"},{"issue":"7","key":"2381_CR13","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1007\/s12094-017-1805-0","volume":"20","author":"M Wang","year":"2018","unstructured":"Wang M, Ji S, Shao G, Zhang J, Zhao K, Wang Z, et al. Effect of exosome biomarkers for diagnosis and prognosis of breast cancer patients. Clin Transl Oncol. 2018;20(7):906\u201311. https:\/\/doi.org\/10.1007\/s12094-017-1805-0.","journal-title":"Clin Transl Oncol"},{"issue":"14","key":"2381_CR14","doi-asserted-by":"publisher","first-page":"2167","DOI":"10.1002\/1873-3468.12722","volume":"591","author":"G Deng","year":"2017","unstructured":"Deng G, Qu J, Zhang Y, Che X, Cheng Y, Fan Y, et al. Gastric cancer-derived exosomes promote peritoneal metastasis by destroying the mesothelial barrier. FEBS Lett. 2017;591(14):2167\u201379. https:\/\/doi.org\/10.1002\/1873-3468.12722.","journal-title":"FEBS Lett"},{"issue":"3","key":"2381_CR15","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1158\/0008-5472.CAN-16-0964","volume":"77","author":"M Tanaka","year":"2017","unstructured":"Tanaka M, Kuriyama S, Itoh G, Maeda D, Goto A, Tamiya Y, et al. Mesothelial cells create a novel tissue niche that facilitates gastric cancer invasion. Cancer Res. 2017;77(3):684\u201395. https:\/\/doi.org\/10.1158\/0008-5472.CAN-16-0964.","journal-title":"Cancer Res"},{"issue":"10","key":"2381_CR16","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1080\/15384101.2020.1749467","volume":"19","author":"M Zhu","year":"2020","unstructured":"Zhu M, Zhang N, He S, Lu X. Exosomal miR-106a derived from gastric cancer promotes peritoneal metastasis via direct regulation of Smad7. Cell Cycle. 2020;19(10):1200\u201321. https:\/\/doi.org\/10.1080\/15384101.2020.1749467.","journal-title":"Cell Cycle"},{"issue":"1","key":"2381_CR17","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1186\/s12957-018-1409-3","volume":"16","author":"T Li","year":"2018","unstructured":"Li T, Gao X, Han L, Yu J, Li H. Identification of hub genes with prognostic values in gastric cancer by bioinformatics analysis. World J Surg Oncol. 2018;16(1):114. https:\/\/doi.org\/10.1186\/s12957-018-1409-3.","journal-title":"World J Surg Oncol"},{"issue":"2","key":"2381_CR18","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1158\/2326-6066.CIR-19-0522","volume":"8","author":"V Vignard","year":"2020","unstructured":"Vignard V, Labbe M, Marec N, et al. MicroRNAs in tumor exosomes drive immune escape in melanoma. Cancer Immunol Res. 2020;8(2):255\u201367. https:\/\/doi.org\/10.1158\/2326-6066.CIR-19-0522.","journal-title":"Cancer Immunol Res"},{"issue":"4","key":"2381_CR19","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1172\/JCI81136","volume":"126","author":"TL Whiteside","year":"2016","unstructured":"Whiteside TL. Exosomes and tumor-mediated immune suppression. J Clin Invest. 2016;126(4):1216\u201323. https:\/\/doi.org\/10.1172\/JCI81136.","journal-title":"J Clin Invest"},{"issue":"1","key":"2381_CR20","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1186\/s12957-020-01846-3","volume":"18","author":"H Zhang","year":"2020","unstructured":"Zhang H, Li L, Yuan C, Wang C, Gao T, Zheng Z. MiR-489 inhibited the development of gastric cancer via regulating HDAC7 and PI3K\/AKT pathway. World J Surg Oncol. 2020;18(1):73. https:\/\/doi.org\/10.1186\/s12957-020-01846-3.","journal-title":"World J Surg Oncol"},{"issue":"2","key":"2381_CR21","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1002\/stem.1571","volume":"32","author":"Y Feng","year":"2014","unstructured":"Feng Y, Huang W, Meng W, Jegga AG, Wang Y, Cai W, et al. Heat shock improves Sca-1+ stem cell survival and directs ischemic cardiomyocytes toward a prosurvival phenotype via exosomal transfer: a critical role for HSF1\/miR-34a\/HSP70 pathway. Stem Cells. 2014;32(2):462\u201372. https:\/\/doi.org\/10.1002\/stem.1571.","journal-title":"Stem Cells"},{"issue":"1","key":"2381_CR22","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1186\/s12957-016-0985-3","volume":"14","author":"T Okumura","year":"2016","unstructured":"Okumura T, Kojima H, Miwa T, Sekine S, Hashimoto I, Hojo S, et al. The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma. World J Surg Oncol. 2016;14(1):228. https:\/\/doi.org\/10.1186\/s12957-016-0985-3.","journal-title":"World J Surg Oncol"},{"issue":"1","key":"2381_CR23","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1186\/s12957-015-0513-x","volume":"13","author":"Z Zhao","year":"2015","unstructured":"Zhao Z, Wang L, Song W, Cui H, Chen G, Qiao F, et al. Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer. World J Surg Oncol. 2015;13(1):101. https:\/\/doi.org\/10.1186\/s12957-015-0513-x.","journal-title":"World J Surg Oncol"},{"issue":"1","key":"2381_CR24","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1186\/s12957-021-02274-7","volume":"19","author":"Q Meng","year":"2021","unstructured":"Meng Q, Wang N, Duan G. Long non-coding RNA XIST regulates ovarian cancer progression via modulating miR-335\/BCL2L2 axis. World J Surg Oncol. 2021;19(1):165. https:\/\/doi.org\/10.1186\/s12957-021-02274-7.","journal-title":"World J Surg Oncol"},{"issue":"1","key":"2381_CR25","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1186\/s12957-020-01896-7","volume":"18","author":"J Zhang","year":"2020","unstructured":"Zhang J, Zhang Y, Cheng S, Mu Y, Liu Y, Yi X, et al. LAIR-1 overexpression inhibits epithelial\u2013mesenchymal transition in osteosarcoma via GLUT1-related energy metabolism. World J Surg Oncol. 2020;18(1):136. https:\/\/doi.org\/10.1186\/s12957-020-01896-7.","journal-title":"World J Surg Oncol"},{"issue":"1","key":"2381_CR26","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1186\/1477-7819-9-172","volume":"9","author":"J Zhou","year":"2011","unstructured":"Zhou J, Zhang L, Gu Y, Li K, Nie Y, Fan D, et al. Dynamic expression of CEACAM7 in precursor lesions of gastric carcinoma and its prognostic value in combination with CEA. World J Surg Oncol. 2011;9(1):172. https:\/\/doi.org\/10.1186\/1477-7819-9-172.","journal-title":"World J Surg Oncol"},{"issue":"4","key":"2381_CR27","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1093\/jb\/mvy047","volume":"164","author":"M Saitoh","year":"2018","unstructured":"Saitoh M. Involvement of partial EMT in cancer progression. J Biochem. 2018;164(4):257\u201364. https:\/\/doi.org\/10.1093\/jb\/mvy047.","journal-title":"J Biochem"},{"issue":"1-2","key":"2381_CR28","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s10555-008-9169-0","volume":"28","author":"M Yilmaz","year":"2009","unstructured":"Yilmaz M, Christofori G. EMT, the cytoskeleton, and cancer cell invasion. Cancer Metastasis Rev. 2009;28(1-2):15\u201333. https:\/\/doi.org\/10.1007\/s10555-008-9169-0.","journal-title":"Cancer Metastasis Rev"},{"issue":"5","key":"2381_CR29","first-page":"362","volume":"172","author":"D Menzies","year":"1991","unstructured":"Menzies D, Ellis H. The role of plasminogen activator in adhesion prevention. Surg Gynecol Obstet. 1991;172(5):362\u20136.","journal-title":"Surg Gynecol Obstet"},{"issue":"6","key":"2381_CR30","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1038\/ncb2976","volume":"16","author":"A Puisieux","year":"2014","unstructured":"Puisieux A, Brabletz T, Caramel J. Oncogenic roles of EMT-inducing transcription factors. Nat Cell Biol. 2014;16(6):488\u201394. https:\/\/doi.org\/10.1038\/ncb2976.","journal-title":"Nat Cell Biol"},{"issue":"2","key":"2381_CR31","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/s41580-018-0080-4","volume":"20","author":"A Dongre","year":"2019","unstructured":"Dongre A, Weinberg RA. New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer. Nat Rev Mol Cell Biol. 2019;20(2):69\u201384. https:\/\/doi.org\/10.1038\/s41580-018-0080-4.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"Suppl 3","key":"2381_CR32","doi-asserted-by":"publisher","first-page":"S1436","DOI":"10.1245\/s10434-015-4418-2","volume":"22","author":"S Sakimura","year":"2015","unstructured":"Sakimura S, Sugimachi K, Kurashige J, et al. The miR-506-induced epithelial-mesenchymal transition is involved in poor prognosis for patients with gastric cancer. Ann Surg Oncol. 2015;22(Suppl 3):S1436\u201343.","journal-title":"Ann Surg Oncol"},{"issue":"4","key":"2381_CR33","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1159\/000490871","volume":"47","author":"LQ Li","year":"2018","unstructured":"Li LQ, Pan D, Chen Q, Zhang SW, Xie DY, Zheng XL, et al. Sensitization of gastric cancer cells to 5-FU by microRNA-204 through targeting the TGFBR2-mediated epithelial to mesenchymal transition. Cell Physiol Biochem. 2018;47(4):1533\u201345. https:\/\/doi.org\/10.1159\/000490871.","journal-title":"Cell Physiol Biochem"},{"key":"2381_CR34","first-page":"2732057","volume":"2019","author":"J He","year":"2019","unstructured":"He J, Xiao B, Li X, He Y, Li L, Sun Z. MiR-486-5p suppresses proliferation and migration of hepatocellular carcinoma cells through downregulation of the E3 ubiquitin ligase CBL. Biomed Res Int. 2019;2019:2732057.","journal-title":"Biomed Res Int"},{"issue":"19","key":"2381_CR35","doi-asserted-by":"publisher","first-page":"4695","DOI":"10.7150\/jca.30596","volume":"10","author":"H Li","year":"2019","unstructured":"Li H, Mou Q, Li P, Yang Z, Wang Z, Niu J, et al. MiR-486-5p inhibits IL-22-induced epithelial-mesenchymal transition of breast cancer cell by repressing Dock1. J Cancer. 2019;10(19):4695\u2013706. https:\/\/doi.org\/10.7150\/jca.30596.","journal-title":"J Cancer"},{"issue":"8","key":"2381_CR36","doi-asserted-by":"publisher","first-page":"13747","DOI":"10.1002\/jcp.28054","volume":"234","author":"A Liu","year":"2019","unstructured":"Liu A, Liu L, Lu H. LncRNA XIST facilitates proliferation and epithelial-mesenchymal transition of colorectal cancer cells through targeting miR-486-5p and promoting neuropilin-2. J Cell Physiol. 2019;234(8):13747\u201361. https:\/\/doi.org\/10.1002\/jcp.28054.","journal-title":"J Cell Physiol"},{"key":"2381_CR37","doi-asserted-by":"publisher","first-page":"6909","DOI":"10.2147\/OTT.S117338","volume":"9","author":"X Zhang","year":"2016","unstructured":"Zhang X, Zhang T, Yang K, Zhang M, Wang K. miR-486-5p suppresses prostate cancer metastasis by targeting Snail and regulating epithelial-mesenchymal transition. Onco Targets Ther. 2016;9:6909\u201314.","journal-title":"Onco Targets Ther"}],"container-title":["World Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12957-021-02381-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12957-021-02381-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12957-021-02381-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T21:04:56Z","timestamp":1634936696000},"score":1,"resource":{"primary":{"URL":"https:\/\/wjso.biomedcentral.com\/articles\/10.1186\/s12957-021-02381-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,23]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["2381"],"URL":"https:\/\/doi.org\/10.1186\/s12957-021-02381-5","relation":{},"ISSN":["1477-7819"],"issn-type":[{"value":"1477-7819","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,23]]},"assertion":[{"value":"24 May 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"312"}}