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This study aimed at evaluating the protective or ameliorative effect of ICA against reproductive toxicity induced by intraperitoneal injection of nicotine in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using simple random allocation, forty male mice were randomly divided into 4 groups: control (received 0.35\u2009mL physiological saline via gastric gavage), nicotine (0.75\u2009mg\/kg BW\/day intraperitoneally), ICA (75\u2009mg\/kg BW\/day gastric gavage), and nicotine plus ICA (nicotine, 0.75\u2009mg\/kg BW\/day intraperitoneally + ICA, 75\u2009mg\/kg BW\/day gastric gavage) group. After 35\u2009days of treatment, the mice were weighed, sacrificed, and their reproductive organs (testis and epididymis) were collected and examined for further studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The nicotine-treated group showed significantly decreased epididymal sperm density and serum testosterone concentration relative to the control group. Nicotine also caused oxidative damage shown by significant reduction in the activities of antioxidant enzymes and elevation in Malondialdehyde (MDA) levels. ICA on the other hand, improved the reduction in sperm density, hormone levels, and activities of antioxidant enzymes altered in the nicotine treated mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings indicate that nicotine-induced reproductive toxicity and oxidative damage on male reproductive tissues could be attenuated by ICA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12958-020-00620-0","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T05:02:36Z","timestamp":1592456556000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of the protective effects of icariin on nicotine-induced reproductive toxicity in male mouse \u2013a pilot study"],"prefix":"10.1186","volume":"18","author":[{"given":"Guochao","family":"Ni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth Yaw","family":"Afedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Rui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"620_CR1","doi-asserted-by":"publisher","first-page":"24","DOI":"10.4103\/0971-5851.151771","volume":"36","author":"A Mishra","year":"2015","unstructured":"Mishra A, Chaturvedi P, Datta S, Sinukumar S, Joshi P, Garg A. 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