{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:05:06Z","timestamp":1785506706860,"version":"3.56.0"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T00:00:00Z","timestamp":1579305600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T00:00:00Z","timestamp":1579305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod Biol Endocrinol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Human embryos are usually cultured to blastocyst stage by Day 5 or 6 after insemination. However, some embryos grow slowly and reach blastocyst stage at Day 7. Acceptable live birth rates have been reported after transfer of Day 7 blastocysts resulted from fresh oocyte in vitro fertilization\u00a0(IVF). It is unknown whether an extended embryo culture to Day 7 is necessary for cryopreserved oocyte IVF to obtain more transferrable blastocysts.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>In this study, 455 oocytes from 57\u2009cycles were warmed, inseminated, and the resulting embryos were cultured by Day 7 to examine blastocyst development after extended culture. Fifty one blastocysts from 16\u2009cycles were biopsied to examine embryo aneuploidies.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>It was found that 35.1% of the cycles had Day 7 blastocysts, and 3.5% of the cycles had only Day 7 blastocysts. Day 7 blastocysts accounted for 15.6% of total blastocysts. The proportion of top quality of blastocysts was lower at Day 7 than at Day 5 or 6. However, no differences were observed on aneuploid blastocyst rates among Days 5, 6 and 7. Similar clinical pregnancy, ongoing pregnancy and embryo implantation rates were obtained after Day 7 blastocyst transfer as compared with Day 5 or 6 blastocyst transfer.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>These results indicate that embryos from oocyte warming cycles should be cultured to Day 7 if they do not reach to blastocyst stage by Day 6 so that number of usable blastocysts can be increased.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12958-020-0565-9","type":"journal-article","created":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T05:03:51Z","timestamp":1579323831000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Is day 7 culture necessary for in vitro fertilization of cryopreserved\/warmed human oocytes?"],"prefix":"10.1186","volume":"18","author":[{"given":"Xiangli","family":"Niu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassie T.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghassan","family":"Haddad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6267-8106","authenticated-orcid":false,"given":"Weihua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,18]]},"reference":[{"key":"565_CR1","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/j.fertnstert.2015.11.027","volume":"105","author":"A Cobo","year":"2016","unstructured":"Cobo A, Garc\u00eda-Velasco JA, Coello A, Domingo J, Pellicer A, Remoh\u00ed J. Oocyte vitrification as an efficient option for elective fertility preservation. Fertil Steril. 2016;105:755\u201364.e8. https:\/\/doi.org\/10.1016\/j.fertnstert.2015.11.027 Epub 2015 Dec 10.","journal-title":"Fertil Steril"},{"key":"565_CR2","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1016\/j.fertnstert.2015.10.026","volume":"105","author":"JO Doyle","year":"2016","unstructured":"Doyle JO, Richter KS, Lim J, Stillman RJ, Graham JR, Tucker MJ. Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Fertil Steril. 2016;105:459\u201366.e2. https:\/\/doi.org\/10.1016\/j.fertnstert.2015.10.026 Epub 2015 Nov 18.","journal-title":"Fertil Steril"},{"key":"565_CR3","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/S0303-7207(00)00365-8","volume":"169","author":"D Meirow","year":"2000","unstructured":"Meirow D. Reproduction post-chemotherapy in young cancer patients. Mol Cell Endocrinol. 2000;169:123\u201331.","journal-title":"Mol Cell Endocrinol"},{"key":"565_CR4","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1007\/978-1-4939-6828-2_14","volume":"1568","author":"J Graham","year":"2017","unstructured":"Graham J, Lim J, Tucker M. Establishing an oocyte cryobank network. Methods Mol Biol. 2017;1568:195\u2013203. https:\/\/doi.org\/10.1007\/978-1-4939-6828-2_14.","journal-title":"Methods Mol Biol"},{"issue":"3","key":"565_CR5","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j. fertnstert.2017.06.024","volume":"108","author":"A Cobo","year":"2017","unstructured":"Cobo A, Coello A, Remoh\u00ed J, Serrano J, de Los Santos JM, Meseguer M. Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation. Fertil Steril. 2017;108(3):491\u2013497.e3. https:\/\/doi.org\/10.1016\/j.%20fertnstert.2017.06.024.","journal-title":"Fertil Steril"},{"key":"565_CR6","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1093\/humrep\/dey091","volume":"33","author":"ER Hammond","year":"2018","unstructured":"Hammond ER, Cree LM, Morbeck DE. Should extended blastocyst culture include day 7? Hum Reprod. 2018;33:991\u20137. https:\/\/doi.org\/10.1093\/humrep\/dey091.","journal-title":"Hum Reprod"},{"key":"565_CR7","first-page":"4","volume":"6","author":"K Hiraoka","year":"2009","unstructured":"Hiraoka K, Hiraoka K, Miyazaki M, Fukunaga E, Horiuchi T, Kusuda T, Okano S, Kinutani M, Kinutani K. Perinatal outcomes following transfer of human blastocysts vitrified at day 5, 6 and 7. J Exp Clin Assist Reprod. 2009;6:4.","journal-title":"J Exp Clin Assist Reprod"},{"key":"565_CR8","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1016\/j.fertnstert.2013.06.021","volume":"100","author":"G Kovalevsky","year":"2013","unstructured":"Kovalevsky G, Carney SM, Morrison LS, Boylan CF, Neithardt AB, Feinberg RF. Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates. Fertil Steril. 2013;100:1008\u201312. https:\/\/doi.org\/10.1016\/j.fertnstert.2013.06.021 Epub 2013 Jul 19.","journal-title":"Fertil Steril"},{"key":"565_CR9","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s12958-016-0157-x","volume":"14","author":"Y Su","year":"2016","unstructured":"Su Y, Li JJ, Wang C, Haddad G, Wang WH. Aneuploidy analysis in day 7 human blastocysts produced by in vitro fertilization. Reprod Biol Endocrinol. 2016;14:20. https:\/\/doi.org\/10.1186\/s12958-016-0157-x.","journal-title":"Reprod Biol Endocrinol"},{"key":"565_CR10","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1093\/humrep\/dey092","volume":"33","author":"T Du","year":"2018","unstructured":"Du T, Wang Y, Fan Y, Zhang S, Yan Z, Yu W, Xi Q, Chen Q, Mol BW, Lyu Q, Kuang Y. Fertility and neonatal outcomes of embryos achieving blastulation on day 7: are they of clinical value? Hum Reprod. 2018;33:1038\u201351. https:\/\/doi.org\/10.1093\/humrep\/dey092.","journal-title":"Hum Reprod"},{"key":"565_CR11","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1093\/humrep\/dew183","volume":"31","author":"MG Minasi","year":"2016","unstructured":"Minasi MG, Colasante A, Riccio T, Ruberti A, Casciani V, Scarselli F, Spinella F, Fiorentino F, Varricchio MT, Greco E. Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study. Hum Reprod. 2016;31:2245\u201354. https:\/\/doi.org\/10.1093\/humrep\/dew183 Epub 2016 Sep 2.","journal-title":"Hum Reprod"},{"key":"565_CR12","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/s10815-017-1038-8","volume":"35","author":"A Kaing","year":"2018","unstructured":"Kaing A, Kroener LL, Tassin R, Li M, Liu L, Buyalos R, Hubert G, Shamonki M. Earlier day of blastocyst development is predictive of embryonic euploidy across all ages: essential data for physician decision-making and counseling patients. J Assist Reprod Genet. 2018;35:119\u201325. https:\/\/doi.org\/10.1007\/s10815-017-1038-8 Epub 2017 Sep 11.","journal-title":"J Assist Reprod Genet"},{"key":"565_CR13","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1080\/14647273.2016.1223357","volume":"19","author":"J Subira","year":"2016","unstructured":"Subira J, Craig J, Turner K, Bevan A, Ohuma E, McVeigh E, Child T, Fatum M. Grade of the inner cell mass, but not trophectoderm, predicts live birth in fresh blastocyst single transfers. Hum Fertil (Camb). 2016;19:254\u201361 Epub 2016 Sep 14.","journal-title":"Hum Fertil (Camb)"},{"key":"565_CR14","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1016\/j.fertnstert.2012.12.003","volume":"99","author":"MJ Hill","year":"2013","unstructured":"Hill MJ, Richter KS, Heitmann RJ, Graham JR, Tucker MJ, DeCherney AH, Browne PE, Levens ED. Trophectoderm grade predicts outcomes of single-blastocyst transfers. Fertil Steril. 2013;99:1283\u20131289.e1. https:\/\/doi.org\/10.1016\/j.fertnstert.2012.12.003 Epub 2013 Jan 8.","journal-title":"Fertil Steril"},{"key":"565_CR15","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1016\/j.fertnstert. 2018.07.013","volume":"110","author":"AM Quaas","year":"2018","unstructured":"Quaas AM, Pennings G. The current status of oocyte banks: domestic and international perspectives. Fertil Steril. 2018;110:1203\u20138. https:\/\/doi.org\/10.1016\/j.fertnstert.%202018.07.013.","journal-title":"Fertil Steril"}],"container-title":["Reproductive Biology and Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12958-020-0565-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12958-020-0565-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12958-020-0565-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T00:06:31Z","timestamp":1610841991000},"score":1,"resource":{"primary":{"URL":"https:\/\/rbej.biomedcentral.com\/articles\/10.1186\/s12958-020-0565-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,18]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["565"],"URL":"https:\/\/doi.org\/10.1186\/s12958-020-0565-9","relation":{},"ISSN":["1477-7827"],"issn-type":[{"value":"1477-7827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,18]]},"assertion":[{"value":"27 September 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 January 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All patients are recipients who received donated frozen oocytes from our donor oocyte bank. Patients signed written consents for all laboratory and clinical procedures. The data were retrospectively collected from the medical records so the IRB was waived for this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"4"}}