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Preliminary evidence revealed that in women undergoing IVF treatment, urinary BPA levels were associated with low serum anti-Mullerian hormone, however a definitive relationship between the two has not yet been characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study aimed to evaluate BPA and BPS effects on in vitro oocyte maturation and early preimplantation embryo development through i) analysis of anti-Mullerian hormone (AMH) and anti-Mullerian hormone receptor II (AMHRII), ii) investigation of developmental parameters, such as cleavage, blastocyst rates and developmental arrest, iii) detection of apoptosis and iv) assessment of possible sex ratio skew. An in vitro bovine model was used as a translational model for human early embryonic development. We first assessed AMH and AMHRII levels after bisphenol exposure during oocyte maturation. Zygotes were also analyzed during cleavage and blastocysts stages. Techniques used include in vitro fertilization, quantitative polymerase chain reaction (qPCR), western blotting, TUNEL and immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our findings show that BPA significantly decreased cleavage (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), blastocyst (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.005) and overall developmental rates as well as significantly increased embryonic arrest at the 2\u20134 cell stage (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Additionally, both BPA and BPS significantly increased DNA fragmentation in 2\u20134 cells, 8\u201316 cells and blastocyst embryos (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, BPA and BPS alter AMH and AMHRII at the mRNA and protein level in both oocytes and blastocysts. BPA, but not BPS, also significantly skews sex ratios towards female blastocysts (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study shows that BPA affects AMH and AMHRII expression during oocyte maturation and that BPS exerts its effects to a greater extent after fertilization and therefore may not be a safer alternative to BPA. Our data lay the foundation for future functional studies, such as receptor kinetics, downstream effectors, and promoter activation\/inhibition to prove a functional relationship between bisphenols and the AMH signalling system.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12958-021-00773-6","type":"journal-article","created":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T00:02:48Z","timestamp":1627948968000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["BPA and BPS affect the expression of anti-Mullerian hormone (AMH) and its receptor during bovine oocyte maturation and early embryo development"],"prefix":"10.1186","volume":"19","author":[{"given":"Angela Christina","family":"Saleh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem","family":"Sabry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Fabiana","family":"Mastromonaco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5757-568X","authenticated-orcid":false,"given":"Laura Alessandra","family":"Favetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,3]]},"reference":[{"issue":"3","key":"773_CR1","doi-asserted-by":"publisher","first-page":"e0120330","DOI":"10.1371\/journal.pone.0120330","volume":"10","author":"L Li","year":"2015","unstructured":"Li L, Wang Q, Zhang Y, Niu Y, Yao X, Liu H. 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