{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T09:08:02Z","timestamp":1767172082349,"version":"build-2238731810"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T00:00:00Z","timestamp":1624665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T00:00:00Z","timestamp":1624665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"NHC Key Laboratory of Male Reproduction and Genetics","award":["KF201902"],"award-info":[{"award-number":["KF201902"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod Biol Endocrinol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Otogelin-like protein (encoded by\n                    <jats:italic>Otogl<\/jats:italic>\n                    ) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99\u2009%). Then, we examined the expression of OTOGL in developing mouse testes.\n                    <jats:italic>Otogl<\/jats:italic>\n                    mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global\n                    <jats:italic>Otogl<\/jats:italic>\n                    knockout mouse (KO) model using the CRISPR\/Cas9 technology; however,\n                    <jats:italic>Otogl<\/jats:italic>\n                    KO mice displayed normal development and fertility. Further histological analysis of\n                    <jats:italic>Otogl<\/jats:italic>\n                    knockout mouse testes revealed that all types of spermatogenic cells are present in\n                    <jats:italic>Otogl<\/jats:italic>\n                    KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis.\n                  <\/jats:p>","DOI":"10.1186\/s12958-021-00779-0","type":"journal-article","created":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T05:02:37Z","timestamp":1624683757000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice"],"prefix":"10.1186","volume":"19","author":[{"given":"Zhiming","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congcong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoting","family":"Gui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunge","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1460-7682","authenticated-orcid":false,"given":"Shuiqiao","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,6,26]]},"reference":[{"key":"779_CR1","doi-asserted-by":"publisher","first-page":"2783","DOI":"10.1093\/humrep\/del164","volume":"21","author":"CL Russo","year":"2006","unstructured":"Russo CL, Spurr-Michaud S, Tisdale A, Pudney J, Anderson D, Gipson IK. 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All experiments with mice were conducted ethically according to the Guide for the Care and Use of Laboratory Animal guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no completing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"95"}}