{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:15:28Z","timestamp":1791490528327,"version":"4.3.4"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2703000"],"award-info":[{"award-number":["2022YFC2703000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC2706600"],"award-info":[{"award-number":["2024YFC2706600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China to J.Z.","award":["32200704"],"award-info":[{"award-number":["32200704"]}]},{"name":"2115 Talent Development Pogram of China Agricultural University to Y.Z."},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371664"],"award-info":[{"award-number":["82371664"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Program of National Natural Science Foundation of China to H.Z.","award":["82230051"],"award-info":[{"award-number":["82230051"]}]},{"name":"Innovative Project of State Key Laboratory of Animal Biotech Breeding to H.Z.","award":["2023SKLAB1-6"],"award-info":[{"award-number":["2023SKLAB1-6"]}]},{"name":"Chinese Universities Scientific Fund to H.Z.","award":["2024TC017"],"award-info":[{"award-number":["2024TC017"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod Biol Endocrinol"],"DOI":"10.1186\/s12958-025-01422-y","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T02:17:03Z","timestamp":1751509023000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A dual-drug strategy to enhance the function of cryopreserved ovaries by promoting revascularization and inhibiting follicle over-activation"],"prefix":"10.1186","volume":"23","author":[{"given":"Lingyu","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longzhong","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuebing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiying","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxian","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqiang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"6","key":"1422_CR1","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1093\/humupd\/dmz027","volume":"25","author":"N Spears","year":"2019","unstructured":"Spears N, Lopes F, Stefansdottir A, Rossi V, De Felici M, Anderson RA, et al. Ovarian damage from chemotherapy and current approaches to its protection. Hum Reprod Update. 2019;25(6):673\u201393.","journal-title":"Hum Reprod Update"},{"issue":"10","key":"1422_CR2","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1016\/S1470-2045(14)70334-1","volume":"15","author":"WH Wallace","year":"2014","unstructured":"Wallace WH, Smith AG, Kelsey TW, Edgar AE, Anderson RA. Fertility preservation for girls and young women with cancer: population-based validation of criteria for ovarian tissue cryopreservation. Lancet Oncol. 2014;15(10):1129\u201336.","journal-title":"Lancet Oncol"},{"issue":"4","key":"1422_CR3","first-page":"400","volume":"378","author":"J Donnez","year":"2018","unstructured":"Donnez J, Dolmans MM. Fertility preservation in women. N Engl J Med. 2018;378(4):400\u20131.","journal-title":"N Engl J Med"},{"issue":"3","key":"1422_CR4","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1093\/humupd\/dmac003","volume":"28","author":"H Khattak","year":"2022","unstructured":"Khattak H, Malhas R, Craciunas L, Afifi Y, Amorim CA, Fishel S, et al. Fresh and cryopreserved ovarian tissue transplantation for preserving reproductive and endocrine function: a systematic review and individual patient data meta-analysis. Hum Reprod Update. 2022;28(3):400\u201316.","journal-title":"Hum Reprod Update"},{"issue":"15","key":"1422_CR5","doi-asserted-by":"publisher","first-page":"4513","DOI":"10.3390\/jcm13154513","volume":"13","author":"T Ramirez","year":"2024","unstructured":"Ramirez T, Pavone M. Exploring the frontiers of ovarian tissue cryopreservation: a review. J Clin Med. 2024;13(15):4513.","journal-title":"J Clin Med"},{"issue":"2","key":"1422_CR6","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.fertnstert.2020.03.037","volume":"114","author":"M Shapira","year":"2020","unstructured":"Shapira M, Dolmans MM, Silber S, Meirow D. Evaluation of ovarian tissue transplantation: results from three clinical centers. Fertil Steril. 2020;114(2):388\u201397.","journal-title":"Fertil Steril"},{"issue":"8","key":"1422_CR7","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1007\/s10815-015-0544-9","volume":"32","author":"J Donnez","year":"2015","unstructured":"Donnez J, Dolmans MM. Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice. J Assist Reprod Genet. 2015;32(8):1167\u201370.","journal-title":"J Assist Reprod Genet"},{"key":"1422_CR8","doi-asserted-by":"publisher","first-page":"117955811986735","DOI":"10.1177\/1179558119867357","volume":"13","author":"L Lotz","year":"2019","unstructured":"Lotz L, Dittrich R, Hoffmann I, Beckmann MW. Ovarian tissue transplantation: experience from Germany and worldwide efficacy. Clin Med Insights Reprod Health. 2019;13:1179558119867357.","journal-title":"Clin Med Insights Reprod Health"},{"issue":"1","key":"1422_CR9","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s10815-017-1079-z","volume":"35","author":"Z Gavish","year":"2018","unstructured":"Gavish Z, Spector I, Peer G, Schlatt S, Wistuba J, Roness H, et al. Follicle activation is a significant and immediate cause of follicle loss after ovarian tissue transplantation. J Assist Reprod Gen. 2018;35(1):61\u20139.","journal-title":"J Assist Reprod Gen"},{"issue":"3","key":"1422_CR10","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.fertnstert.2018.04.009","volume":"110","author":"L Cacciottola","year":"2018","unstructured":"Cacciottola L, Manavella DD, Amorim CA, Donnez J, Dolmans MM. In vivo characterization of metabolic activity and oxidative stress in grafted human ovarian tissue using Microdialysis. Fertil Steril. 2018;110(3):534\u201344. e3.","journal-title":"Fertil Steril"},{"issue":"6","key":"1422_CR11","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1093\/humrep\/dey080","volume":"33","author":"DD Manavella","year":"2018","unstructured":"Manavella DD, Cacciottola L, Pomme S, Desmet CM, Jordan BF, Donnez J, et al. Two-step transplantation with adipose tissue-derived stem cells increases follicle survival by enhancing vascularization in xenografted frozen-thawed human ovarian tissue. Hum Reprod. 2018;33(6):1107\u201316.","journal-title":"Hum Reprod"},{"issue":"6","key":"1422_CR12","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1093\/humrep\/del010","volume":"21","author":"T Israely","year":"2006","unstructured":"Israely T, Nevo N, Harmelin A, Neeman M, Tsafiri A. Reducing ischaemic damage in rodent ovarian xenografts transplanted into granulation tissue. Hum Reprod. 2006;21(6):1368\u201379.","journal-title":"Hum Reprod"},{"issue":"4","key":"1422_CR13","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1093\/molehr\/gaz008","volume":"25","author":"DD Manavella","year":"2019","unstructured":"Manavella DD, Cacciottola L, Payen VL, Amorim CA, Donnez J, Dolmans MM. Adipose tissue-derived stem cells boost vascularization in grafted ovarian tissue by growth factor secretion and differentiation into endothelial cell lineages. Mol Hum Reprod. 2019;25(4):184\u201393.","journal-title":"Mol Hum Reprod"},{"issue":"6","key":"1422_CR14","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1002\/jemt.20305","volume":"69","author":"G Macchiarelli","year":"2006","unstructured":"Macchiarelli G, Jiang JY, Nottola SA, Sato E. Morphological patterns of angiogenesis in ovarian follicle capillary networks. A scanning electron microscopy study of corrosion cast. Microsc Res Tech. 2006;69(6):459\u201368.","journal-title":"Microsc Res Tech"},{"issue":"2","key":"1422_CR15","doi-asserted-by":"publisher","first-page":"eabi8683","DOI":"10.1126\/sciadv.abi8683","volume":"8","author":"X Xu","year":"2022","unstructured":"Xu X, Mu L, Li L, Liang J, Zhang S, Jia L, et al. Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging. Sci Adv. 2022;8(2):eabi8683.","journal-title":"Sci Adv"},{"issue":"10","key":"1422_CR16","doi-asserted-by":"publisher","first-page":"3845","DOI":"10.1210\/jcem.84.10.6025","volume":"84","author":"N Otani","year":"1999","unstructured":"Otani N, Minami S, Yamoto M, Shikone T, Otani H, Nishiyama R, et al. The vascular endothelial growth factor\/fms-like tyrosine kinase system in human ovary during the menstrual cycle and early pregnancy. J Clin Endocrinol Metab. 1999;84(10):3845\u201351.","journal-title":"J Clin Endocrinol Metab"},{"issue":"3","key":"1422_CR17","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1210\/endo.134.3.8119153","volume":"134","author":"GA Dissen","year":"1994","unstructured":"Dissen GA, Lara HE, Fahrenbach WH, Costa ME, Ojeda SR. Immature rat ovaries become revascularized rapidly after autotransplantation and show a gonadotropin-dependent increase in angiogenic factor gene-expression. Endocrinology. 1994;134(3):1146\u201354.","journal-title":"Endocrinology"},{"issue":"2","key":"1422_CR18","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s10815-015-0628-6","volume":"33","author":"BJ Kang","year":"2016","unstructured":"Kang BJ, Wang Y, Zhang L, Xiao Z, Li SW. bFGF and VEGF improve the quality of vitrified-thawed human ovarian tissues after xenotransplantation to SCID mice. J Assist Reprod Genet. 2016;33(2):281\u20139.","journal-title":"J Assist Reprod Genet"},{"issue":"2","key":"1422_CR19","first-page":"141","volume":"2","author":"L Cacciottola","year":"2021","unstructured":"Cacciottola L, Nguyen TYT, Amorim CA, Donnez J, Dolmans MM. Modulating hypoxia and oxidative stress in human xenografts using adipose tissue-derived stem cells. F S Sci. 2021;2(2):141\u201352.","journal-title":"F S Sci"},{"issue":"6","key":"1422_CR20","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1093\/humrep\/dey080","volume":"33","author":"DD Manavella","year":"2018","unstructured":"Manavella DD, Cacciottola L, Pomm\u00e9 S, Desmet CM, Jordan BF, Donnez J, et al. Two-step transplantation with adipose tissue-derived stem cells increases follicle survival by enhancing vascularization in xenografted frozen-thawed human ovarian tissue. Hum Reprod. 2018;33(6):1107\u201316.","journal-title":"Hum Reprod"},{"issue":"5","key":"1422_CR21","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1007\/s43032-020-00318-z","volume":"28","author":"C Terren","year":"2021","unstructured":"Terren C, Munaut C. Molecular basis associated with the control of primordial follicle activation during transplantation of cryopreserved ovarian tissue. Reprod Sci. 2021;28(5):1257\u201366.","journal-title":"Reprod Sci"},{"issue":"6","key":"1422_CR22","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1093\/humupd\/dmv037","volume":"21","author":"H Zhang","year":"2015","unstructured":"Zhang H, Liu K. Cellular and molecular regulation of the activation of mammalian primordial follicles: somatic cells initiate follicle activation in adulthood. Hum Reprod Update. 2015;21(6):779\u201386.","journal-title":"Hum Reprod Update"},{"issue":"2","key":"1422_CR23","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.tem.2009.10.001","volume":"21","author":"P Reddy","year":"2010","unstructured":"Reddy P, Zheng W, Liu K. Mechanisms maintaining the dormancy and survival of mammalian primordial follicles. Trends Endocrinol Metab. 2010;21(2):96\u2013103.","journal-title":"Trends Endocrinol Metab"},{"key":"1422_CR24","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.bbrc.2020.10.110","volume":"534","author":"W Liu","year":"2021","unstructured":"Liu W, Zhang J, Wang L, Liang S, Xu B, Ying X, et al. The protective effects of Rapamycin pretreatment on ovarian damage during ovarian tissue cryopreservation and transplantation. Biochem Biophys Res Commun. 2021;534:780\u20136.","journal-title":"Biochem Biophys Res Commun"},{"issue":"8","key":"1422_CR25","first-page":"1474","volume":"59","author":"J Bindels","year":"2023","unstructured":"Bindels J, Squatrito M, Bernet L, Nisolle M, Henry L, Munaut C. The mTOR inhibitor Rapamycin counteracts follicle activation induced by ovarian cryopreservation in murine transplantation models. Med (Kaunas). 2023;59(8):1474.","journal-title":"Med (Kaunas)"},{"issue":"9","key":"1422_CR26","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.1093\/humrep\/dey250","volume":"33","author":"J Grosbois","year":"2018","unstructured":"Grosbois J, Demeestere I. Dynamics of PI3K and Hippo signaling pathways during in vitro human follicle activation. Hum Reprod. 2018;33(9):1705\u201314.","journal-title":"Hum Reprod"},{"issue":"4","key":"1422_CR27","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1093\/hmg\/ddt486","volume":"23","author":"WJ Zheng","year":"2014","unstructured":"Zheng WJ, Zhang H, Gorre N, Risal S, Shen Y, Liu K. Two classes of ovarian primordial follicles exhibit distinct developmental dynamics and physiological functions. Hum Mol Genet. 2014;23(4):920\u20138.","journal-title":"Hum Mol Genet"},{"issue":"1","key":"1422_CR28","doi-asserted-by":"publisher","first-page":"8461","DOI":"10.1038\/s41467-023-44312-w","volume":"14","author":"J Cao","year":"2023","unstructured":"Cao J, Jin L, Yan ZQ, Wang XK, Li YY, Wang Z, et al. Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models. Nat Commun. 2023;14(1):8461.","journal-title":"Nat Commun"},{"issue":"5","key":"1422_CR29","doi-asserted-by":"publisher","first-page":"e182","DOI":"10.1002\/ctm2.182","volume":"10","author":"J Zhang","year":"2020","unstructured":"Zhang J, Yan L, Wang Y, Zhang S, Xu X, Dai Y, et al. In vivo and in vitro activation of dormant primordial follicles by EGF treatment in mouse and human. Clin Transl Med. 2020;10(5):e182.","journal-title":"Clin Transl Med"},{"key":"1422_CR30","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.anireprosci.2016.02.029","volume":"168","author":"A Langbeen","year":"2016","unstructured":"Langbeen A, Ginneken CV, Fransen E, Bosmans E, Leroy J, Bols PEJ. Morphometrical analysis of preantral follicular survival of VEGF-treated bovine ovarian cortex tissue following xenotransplantation in an immune deficient mouse model. Anim Reprod Sci. 2016;168:73\u201385.","journal-title":"Anim Reprod Sci"},{"issue":"4\u20135","key":"1422_CR31","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.diff.2015.10.007","volume":"91","author":"GR Cunha","year":"2016","unstructured":"Cunha GR, Baskin L. Use of sub-renal capsule transplantation in developmental biology. Differentiation. 2016;91(4\u20135):4\u20139.","journal-title":"Differentiation"},{"issue":"6","key":"1422_CR32","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1177\/0192623315570339","volume":"43","author":"MC Cora","year":"2015","unstructured":"Cora MC, Kooistra L, Travlos G. Vaginal cytology of the laboratory rat and mouse: review and criteria for the staging of the estrous cycle using stained vaginal smears. Toxicol Pathol. 2015;43(6):776\u201393.","journal-title":"Toxicol Pathol"},{"issue":"1","key":"1422_CR33","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1038\/s41467-021-22829-2","volume":"12","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Wang Y, Feng X, Zhang S, Xu X, Li L, et al. Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice. Nat Commun. 2021;12(1):2523.","journal-title":"Nat Commun"},{"issue":"15","key":"1422_CR34","doi-asserted-by":"publisher","first-page":"e4104","DOI":"10.21769\/BioProtoc.4104","volume":"11","author":"J Greaney","year":"2021","unstructured":"Greaney J, Subramanian GN, Ye Y, Homer H. Isolation and in vitro culture of mouse oocytes. Bio Protoc. 2021;11(15):e4104.","journal-title":"Bio Protoc"},{"issue":"5","key":"1422_CR35","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","volume":"6","author":"JR Wisniewski","year":"2009","unstructured":"Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample Preparation method for proteome analysis. Nat Methods. 2009;6(5):359\u201362.","journal-title":"Nat Methods"},{"issue":"1","key":"1422_CR36","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","volume":"10","author":"Y Zhou","year":"2019","unstructured":"Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi AH, Tanaseichuk O, et al. Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat Commun. 2019;10(1):1523.","journal-title":"Nat Commun"},{"issue":"1","key":"1422_CR37","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.ydbio.2015.04.006","volume":"403","author":"M Faire","year":"2015","unstructured":"Faire M, Skillern A, Arora R, Nguyen DH, Wang J, Chamberlain C, et al. Follicle dynamics and global organization in the intact mouse ovary. Dev Biol. 2015;403(1):69\u201379.","journal-title":"Dev Biol"},{"issue":"1","key":"1422_CR38","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.ydbio.2008.06.017","volume":"321","author":"GB John","year":"2008","unstructured":"John GB, Gallardo TD, Shirley LJ, Castrillon DH. Foxo3 is a PI3K-dependent molecular switch controlling the initiation of oocyte growth. Dev Biol. 2008;321(1):197\u2013204.","journal-title":"Dev Biol"},{"issue":"1","key":"1422_CR39","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/s12915-022-01318-y","volume":"20","author":"Y Dai","year":"2022","unstructured":"Dai Y, Bo Y, Wang P, Xu X, Singh M, Jia L, et al. Asynchronous embryonic germ cell development leads to a heterogeneity of postnatal ovarian follicle activation and May influence the timing of puberty onset in mice. BMC Biol. 2022;20(1):109.","journal-title":"BMC Biol"},{"issue":"21","key":"1422_CR40","doi-asserted-by":"publisher","first-page":"2501","DOI":"10.1016\/j.cub.2014.09.023","volume":"24","author":"H Zhang","year":"2014","unstructured":"Zhang H, Risal S, Gorre N, Busayavalasa K, Li X, Shen Y, et al. Somatic cells initiate primordial follicle activation and govern the development of dormant oocytes in mice. Curr Biol. 2014;24(21):2501\u20138.","journal-title":"Curr Biol"},{"issue":"2","key":"1422_CR41","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.fertnstert.2020.04.050","volume":"114","author":"MM Dolmans","year":"2020","unstructured":"Dolmans MM, Falcone T, Patrizio P. Importance of patient selection to analyze in vitro fertilization outcome with transplanted cryopreserved ovarian tissue. Fertil Steril. 2020;114(2):279\u201380.","journal-title":"Fertil Steril"},{"issue":"1","key":"1422_CR42","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.ejogrb.2009.11.015","volume":"149","author":"G Rahimi","year":"2010","unstructured":"Rahimi G, Isachenko V, Kreienberg R, Sauer H, Todorov P, Tawadros S, et al. Re-vascularisation in human ovarian tissue after conventional freezing or vitrification and xenotransplantation. Eur J Obstet Gyn R B. 2010;149(1):63\u20137.","journal-title":"Eur J Obstet Gyn R B"},{"issue":"1","key":"1422_CR43","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/S0015-0282(00)00548-3","volume":"74","author":"M Nisolle","year":"2000","unstructured":"Nisolle M, Casanas-Roux F, Qu J, Motta P, Donnez J. Histologic and ultrastructural evaluation of fresh and frozen-thawed human ovarian xenografts in nude mice. Fertil Steril. 2000;74(1):122\u20139.","journal-title":"Fertil Steril"},{"issue":"8024","key":"1422_CR44","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1038\/s41586-024-07698-1","volume":"632","author":"X Pan","year":"2024","unstructured":"Pan X, Li X, Dong L, Liu T, Zhang M, Zhang LN, et al. Tumour vasculature at single-cell resolution. Nature. 2024;632(8024):429\u201336.","journal-title":"Nature"},{"issue":"4","key":"1422_CR45","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1111\/acel.12617","volume":"16","author":"X Dou","year":"2017","unstructured":"Dou X, Sun Y, Li J, Zhang J, Hao D, Liu W, et al. Short-term Rapamycin treatment increases ovarian lifespan in young and middle-aged female mice. Aging Cell. 2017;16(4):825\u201336.","journal-title":"Aging Cell"},{"key":"1422_CR46","doi-asserted-by":"publisher","first-page":"4643","DOI":"10.2147\/OTT.S205160","volume":"12","author":"M Wang","year":"2019","unstructured":"Wang M, Xu Y, Wen GZ, Wang Q, Yuan SM. Rapamycin suppresses angiogenesis and lymphangiogenesis in melanoma by downregulating VEGF-A\/VEGFR-2 and VEGF-C\/VEGFR-3 expression. Onco Targets Ther. 2019;12:4643\u201354.","journal-title":"Onco Targets Ther"},{"key":"1422_CR47","doi-asserted-by":"publisher","first-page":"5049","DOI":"10.2147\/IJN.S373777","volume":"17","author":"XM Liu","year":"2022","unstructured":"Liu XM, Zhu WT, Jia ML, Li YT, Hong Y, Liu ZQ, et al. Rapamycin liposomes combined with 5-Fluorouracil inhibits angiogenesis and tumor growth of APC ((Min\/+)) mice and AOM\/DSS-Induced colorectal Cancer mice. Int J Nanomed. 2022;17:5049\u201361.","journal-title":"Int J Nanomed"},{"issue":"5","key":"1422_CR48","doi-asserted-by":"publisher","first-page":"1724","DOI":"10.1111\/cas.13988","volume":"110","author":"X Li","year":"2019","unstructured":"Li X, Chen C, Dai Y, Huang C, Han Q, Jing L, et al. Cinobufagin suppresses colorectal cancer angiogenesis by disrupting the endothelial mammalian target of rapamycin\/hypoxia-inducible factor 1alpha axis. Cancer Sci. 2019;110(5):1724\u201334.","journal-title":"Cancer Sci"}],"container-title":["Reproductive Biology and Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12958-025-01422-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12958-025-01422-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12958-025-01422-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T03:03:27Z","timestamp":1751511807000},"score":1,"resource":{"primary":{"URL":"https:\/\/rbej.biomedcentral.com\/articles\/10.1186\/s12958-025-01422-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["1422"],"URL":"https:\/\/doi.org\/10.1186\/s12958-025-01422-y","relation":{},"ISSN":["1477-7827"],"issn-type":[{"value":"1477-7827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]},"assertion":[{"value":"24 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were approved by the Committee on the Ethics of Animal Experiments at China Agricultural University, NO. AW11104202-3-1.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"95"}}