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Complications of SARS-CoV-2 infection include vascular problems and increased risk of thrombosis; therefore, studies to identify new drugs for treating SARS-CoV-2 infections that also inhibit thrombosis and minimize the risk of bleeding are required.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Objectives<\/jats:title>\n                <jats:p>To determine whether (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K affects the hemostatic system.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Platelet aggregation was induced by collagen, arachidonic acid, and adenosine diphosphate (ADP) in the Chronolog Lumi-aggregometer. The coagulation activity was evaluated by determining activated partial thromboplastin clotting time (aPTT) and prothrombin time (PT) with (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K (5.0\u2013434.5\u00a0\u00b5g) or 0.9% NaCl. Arterial thrombosis was induced with a 540\u00a0nm laser and 3.5\u201320\u00a0mg kg<jats:sup>\u2212\u20091<\/jats:sup> Rose Bengal in the carotid artery of male C57BL\/6J mice using (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K. Bleeding time was determined in mouse tails immersed in saline at 37\u00a0\u00b0C after (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K (4.0\u00a0mg\/kg and 8.0\u00a0mg\/kg) or saline administration.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>(p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K prolonged the aPTT and PT by blocking kallikrein and FXa-like activities. Moreover, (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K inhibited ADP-, collagen-, and arachidonic acid-induced platelet aggregation in a dose-dependent manner. Further, low concentrations of (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K extended the time to artery occlusion by the formed thrombus. However, (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K did not prolong the bleeding time in the mouse model of arterial thrombosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>These results demonstrate the antithrombotic activity of the peptide (p-BthTX-I)<jats:sub>2<\/jats:sub>\u00a0K possibly by kallikrein inhibition, suggesting its strong biotechnological potential.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12959-022-00436-5","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T11:13:37Z","timestamp":1672658017000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A snake venom-analog peptide that inhibits SARS-CoV-2 and papain-like protease displays antithrombotic activity in mice arterial thrombosis model, without interfering with bleeding time"],"prefix":"10.1186","volume":"21","author":[{"given":"Ruben Siedlarczyk","family":"Nogueira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Ramos","family":"Salu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Goulart","family":"Nardelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Ramalho","family":"Bonturi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Rodrigues","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Humberto","family":"de Abreu Maffei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Maffud","family":"Cilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luiza Vilela","family":"Oliva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,1,2]]},"reference":[{"key":"436_CR1","doi-asserted-by":"crossref","unstructured":"Flaumenhaft R, Enjyoji K, Schmaier AA. 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