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However, the functions of the different P proteins are poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Immunofluorescence staining and western blot were performed to detect the autophagy activity, the form of ring-like structure, and the colocalization of BECN1 and P. Co-immunoprecipitation was performed to detect the interaction between P and BECN1. QRT-PCR and TCID\n                      <jats:sub>50<\/jats:sub>\n                      assay were performed to detect the replication level of RABV. Small interfering RNA was used to detect the autophagy signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that P5 attaches to N-terminal residues 1\u2013139 of BECN1 (beclin1) on the BECN1 ring-like structure through amino acid residues 173\u2013222 of P5. Subsequently, we found that P5-induced autophagosomes did not fuse with lysosomes.\n                      <jats:italic>Becn1<\/jats:italic>\n                      silencing did not recover P5 overexpression-induced promotion of RABV replication. Mechanistically, RABV protein P\u0394N82 (P5) induced incomplete autophagy via the BECN1-mediated signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data indicate that P5 binding to the BECN1 ring benefits RABV replication by inducing BECN1 signaling pathway-dependent incomplete autophagy, which provides a potential target for antiviral drugs against RABV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s12964-020-00644-4","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T04:03:55Z","timestamp":1600401835000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Rabies virus phosphoprotein P5 binding to BECN1 regulates self-replication by BECN1-mediated autophagy signaling pathway"],"prefix":"10.1186","volume":"18","author":[{"given":"Juan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"644_CR1","doi-asserted-by":"publisher","first-page":"e0003709","DOI":"10.1371\/journal.pntd.0003709","volume":"9","author":"K Hampson","year":"2015","unstructured":"Hampson K, Coudeville L, Lembo T, Sambo M, Kieffer A, Attlan M, et al. 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