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Cell injury, and subsequent death trigger inflammation as in myocardial infarction (MI) resulting in myocardial remodeling. Although mechanisms underlying myocardial cell death have been studied so far, the signaling events following single cell death and spontaneous response of connected cells in the myocardial tissue is still barely understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we investigated the effect of laser-induced single cell death on Calcium (Ca<jats:sup>2+<\/jats:sup>) concentrations and transport in myocardial cell clusters in vitro. Spatial and temporal changes in intracellular Ca<jats:sup>2+<\/jats:sup>concentrations [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>were studied using a fluorescent calcium indicator, Fluo-4AM. Spontaneous signaling events following cell death were studied in rat embryonic cardiomyocytes and non-myocytes using separate cell culture systems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell death triggered spontaneous increase in intracellular Ca<jats:sup>2+<\/jats:sup>levels ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) of surrounding cells. The spread of the observed propagating Ca<jats:sup>2+<\/jats:sup>signal was slow and sustained in myocytes while it was rapid and transient in fibroblasts (Fbs). Further, sustained high Ca<jats:sup>2+<\/jats:sup>levels temporarily impaired the contractility in CMs. The cell-type specific effect of ablation was confirmed using separate cultures of CMs and Fbs. Comparing Ca<jats:sup>2+<\/jats:sup>propagation speed in myocytes and fibroblasts, we argue for a diffusion-driven Ca<jats:sup>2+<\/jats:sup>propagation in myocytes, but not in fibroblasts. Radial and sequential Ca<jats:sup>2+<\/jats:sup>diffusion across the CMs through cell\u2013cell contacts and presence of Cx43-based intercellular junctions indicated a gap junction flow of Ca<jats:sup>2+<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings illustrate the spontaneous Ca<jats:sup>2+<\/jats:sup>-mediated functional interplay in myocardial cell clusters upon mechanical injury and, further, the difference in Ca<jats:sup>2+<\/jats:sup>signaling in cardiomyocytes and fibroblasts.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12964-020-00689-5","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T13:02:54Z","timestamp":1609160574000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Calcium mediated functional interplay between myocardial cells upon laser-induced single-cell injury: an in vitro study of cardiac cell death signaling mechanisms"],"prefix":"10.1186","volume":"18","author":[{"given":"Krishna Chander","family":"Sridhar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Hersch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Dreissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Merkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3803-8835","authenticated-orcid":false,"given":"Bernd","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"issue":"1","key":"689_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.yjmcc.2009.06.019","volume":"48","author":"M Baruscotti","year":"2010","unstructured":"Baruscotti M, Barbuti A, Bucchi A. 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