{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:02:24Z","timestamp":1791298944635,"version":"4.1.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81972977 and 81802955"],"award-info":[{"award-number":["81972977 and 81802955"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81972977 and 81802955"],"award-info":[{"award-number":["81972977 and 81802955"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Disciplinary Construction Innovation Team Foundation of Chengdu Medical College","award":["CMC-XK-2103"],"award-info":[{"award-number":["CMC-XK-2103"]}]},{"name":"Sichuan Medical Association","award":["S20011"],"award-info":[{"award-number":["S20011"]}]},{"DOI":"10.13039\/100017520","name":"Chengdu Medical College","doi-asserted-by":"publisher","award":["CYZ18-13 and YCX202013"],"award-info":[{"award-number":["CYZ18-13 and YCX202013"]}],"id":[{"id":"10.13039\/100017520","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The First Affiliated Hospital of Chengdu Medical College","award":["CYFY2017ZD03, CYFY2018ZD02, and CYFY2019ZD06"],"award-info":[{"award-number":["CYFY2017ZD03, CYFY2018ZD02, and CYFY2019ZD06"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic> infection is difficult to diagnose clinically and can develop into invasive pulmonary aspergillosis, which has a high fatality rate. The incidence of <jats:italic>Aspergillus fumigatus<\/jats:italic> infection has increased die to widespread application of radiotherapy technology. However, knowledge regarding <jats:italic>A. fumigatus<\/jats:italic> infection following radiation exposure is limited, and the underlying mechanism remains unclear. In this study, we established a mouse model to explore the effect of radiation on <jats:italic>A. fumigatus<\/jats:italic> infection and the associated mechanisms.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In this study, a mouse model of <jats:italic>A. fumigatus<\/jats:italic> infection after radiation was established by irradiating with 5\u00a0Gy on the chest and instilling 5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\/ml Aspergillus fumigatus conidia into trachea after 24\u00a0h to explore the effect and study its function and mechanism. Mice were compared among the following groups: normal controls (CON), radiation only (RA), infection only (Af), and radiation\u2009+\u2009infection (RA\u2009+\u2009Af). Staining analyses were used to detect infection and damage in lung tissues. Changes in protein and mRNA levels of pyroptosis-related molecules were assessed by western blot analysis and quantitative reverse transcription polymerase chain reaction, respectively. Protein concentrations in the serum and alveolar lavage fluid were also measured. An immunofluorescence colocalization analysis was performed to confirm that NLRP3 inflammasomes activated pyroptosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Radiation destroyed the pulmonary epithelial barrier and significantly increased the pulmonary fungal burden of <jats:italic>A. fumigatus<\/jats:italic>. The active end of caspase-1 and gasdermin D (GSDMD) were highly expressed even after infection. Release of interleukin-18 (IL-18) and interleukin-1\u03b2 (IL-1\u03b2) provided further evidence of pyroptosis. <jats:italic>NLRP3<\/jats:italic> knockout inhibited pyroptosis, which effectively attenuated damage to the pulmonary epithelial barrier and reduced the burden of <jats:italic>A. fumigatus<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our findings indicated that the activation of NLRP3 inflammasomes following radiation exposure increased susceptibility to <jats:italic>A. fumigatus<\/jats:italic> infection. Due to pyroptosis in lung epithelial cells, it resulted in the destruction of the lung epithelial barrier and further damage to lung tissue. Moreover, we found that NLRP3 knockout effectively inhibited the pyroptosis and reducing susceptibility to <jats:italic>A. fumigatus<\/jats:italic> infection and further lung damage. Overall, our results suggest that NLRP3\/GSDMD pathway mediated-pyroptosis in the lungs may be a key event in this process and provide new insights into the underlying mechanism of infection.<\/jats:p>\n                \n              <\/jats:sec>","DOI":"10.1186\/s12964-022-00907-2","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T21:03:57Z","timestamp":1656363837000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3\/GSDMD pathway in pulmonary bronchial epithelia"],"prefix":"10.1186","volume":"20","author":[{"given":"Dong-Ming","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Yang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Hua","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Yi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"907_CR1","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.emc.2013.10.002","volume":"32","author":"DM Christensen","year":"2014","unstructured":"Christensen DM, Iddins CJ, Sugarman SL. 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