{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:13:29Z","timestamp":1785478409045,"version":"3.56.0"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Fundamental Research Funds for the Central Universities","award":["HUST: 2021yjsCXCY122"],"award-info":[{"award-number":["HUST: 2021yjsCXCY122"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82001026"],"award-info":[{"award-number":["82001026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81800134"],"award-info":[{"award-number":["81800134"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31620103909"],"award-info":[{"award-number":["31620103909"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Abnormal platelet activation is a key factor in the occurrence and development of thrombotic diseases. However, the physiological mechanisms that underlie platelet homeostasis remain unclear. Oleic acid, one of the most abundant lipids in the human diet, has potential antithrombotic effects. This study aimed to investigate the effects of oleic acid on platelet activation and thrombosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Platelet aggregation, ATP release, and fibrinogen spread were evaluated to determine the role of oleic acid in platelet activation. A ferric chloride-induced carotid injury model was used to establish the effect of oleic acid on thrombus formation in vivo. Western blotting analysis and transfection experiments were performed to determine the mechanisms involved in this process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Oleic acid inhibited platelet aggregation, granule release, and calcium mobilization. Furthermore, it inhibited the spread of platelets on fibrinogen. We also found that oleic acid delayed arterial thrombosis in mice, as demonstrated in a murine model of ferric chloride-induced carotid artery thrombosis. The molecular mechanism of its inhibition of platelet activity may be through the Syk-PLC\u03b32 and CaMKK\u03b2\/AMPK\u03b1\/VASP pathways. In addition, we demonstrated that the phosphorylation of AMPK at Ser496 was an important mechanism of platelet activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study showed that oleic acid inhibits platelet activation and reduces thrombogenesis by inhibiting the phosphorylation of multiple signaling molecules, offering new insights into the research and development of antiplatelet drugs.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12964-023-01276-0","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T11:03:11Z","timestamp":1696935791000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The mechanism of oleic acid inhibiting platelet activation stimulated by collagen"],"prefix":"10.1186","volume":"21","author":[{"given":"Xianghui","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruirui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangyin","family":"Ming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,10]]},"reference":[{"issue":"5","key":"1276_CR1","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.cell.2020.02.016","volume":"180","author":"I Nemet","year":"2020","unstructured":"Nemet I, Saha PP, Gupta N, et al. A Cardiovascular disease-linked gut microbial metabolite acts via adrenergic receptors. Cell. 2020;180(5):862\u2013877e22.","journal-title":"Cell"},{"key":"1276_CR2","unstructured":"Global. burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: a systematic analysis for the Global Burden of Disease Study 2019 Lancet, 2020. 396(10258): p.\u00a01204\u20131222."},{"issue":"13","key":"1276_CR3","doi-asserted-by":"crossref","first-page":"e88","DOI":"10.1161\/01.CIR.0000086897.15588.4B","volume":"108","author":"D Gregg","year":"2003","unstructured":"Gregg D, Goldschmidt-Clermont PJ. Cardiology patient page. Platelets and cardiovascular disease. Circulation. 2003;108(13):e88\u201390.","journal-title":"Circulation"},{"issue":"3","key":"1276_CR4","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/s41569-018-0110-0","volume":"16","author":"PEJ van der Meijden","year":"2019","unstructured":"van der Meijden PEJ, Heemskerk JWM. Platelet biology and functions: new concepts and clinical perspectives. Nat Rev Cardiol. 2019;16(3):166\u201379.","journal-title":"Nat Rev Cardiol"},{"issue":"8","key":"1276_CR5","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1111\/jth.13755","volume":"15","author":"Z Cheng","year":"2017","unstructured":"Cheng Z, Gao W, Fan X, et al. Extracellular signal-regulated kinase 5 associates with casein kinase II to regulate GPIb-IX-mediated platelet activation via the PTEN\/PI3K\/Akt pathway. J Thromb Haemost. 2017;15(8):1679\u201388.","journal-title":"J Thromb Haemost"},{"issue":"5","key":"1276_CR6","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41573-020-0061-0","volume":"19","author":"N Mackman","year":"2020","unstructured":"Mackman N, Bergmeier W, Stouffer GA, Weitz JI. Therapeutic strategies for thrombosis: new targets and approaches. Nat Rev Drug Discov. 2020;19(5):333\u201352.","journal-title":"Nat Rev Drug Discov"},{"issue":"8","key":"1276_CR7","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nrd3777","volume":"11","author":"CC Blad","year":"2012","unstructured":"Blad CC, Tang C, Offermanns S. G protein-coupled receptors for energy metabolites as new therapeutic targets. Nat Rev Drug Discov. 2012;11(8):603\u201319.","journal-title":"Nat Rev Drug Discov"},{"issue":"8","key":"1276_CR8","doi-asserted-by":"crossref","first-page":"778","DOI":"10.2174\/138161211795428876","volume":"17","author":"J Delgado-Lista","year":"2011","unstructured":"Delgado-Lista J, Garcia-Rios A, Perez-Martinez P, Lopez-Miranda J, Perez-Jimenez F. Olive oil and haemostasis: platelet function, thrombogenesis and fibrinolysis. Curr Pharm Des. 2011;17(8):778\u201385.","journal-title":"Curr Pharm Des"},{"issue":"10","key":"1276_CR9","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.3390\/nu11102283","volume":"11","author":"E Piccinin","year":"2019","unstructured":"Piccinin E, Cariello M, De Santis S, et al. Role of oleic acid in the gut-liver axis: from diet to the regulation of its synthesis via stearoyl-CoA Desaturase 1 (SCD1). Nutrients. 2019;11(10):2283.","journal-title":"Nutrients"},{"issue":"11","key":"1276_CR10","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1161\/01.ATV.14.11.1829","volume":"14","author":"R De Caterina","year":"1994","unstructured":"De Caterina R, Cybulsky MI, Clinton SK, Gimbrone MA Jr, Libby P. The omega-3 fatty acid docosahexaenoate reduces cytokine-induced expression of proatherogenic and proinflammatory proteins in human endothelial cells. Arterioscler Thromb. 1994;14(11):1829\u201336.","journal-title":"Arterioscler Thromb"},{"issue":"3","key":"1276_CR11","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/0021-9150(75)90029-5","volume":"22","author":"G Hornstra","year":"1975","unstructured":"Hornstra G, Lussenburg RN. Relationship between the type of dietary fatty acid and arterial thrombosis tendency in rats. Atherosclerosis. 1975;22(3):499\u2013516.","journal-title":"Atherosclerosis"},{"issue":"2\u20133","key":"1276_CR12","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1054\/plef.2002.0386","volume":"67","author":"M Massaro","year":"2002","unstructured":"Massaro M, Carluccio MA, Paolicchi A, Bosetti F, Solaini G, De Caterina R. Mechanisms for reduction of endothelial activation by oleate: inhibition of nuclear factor-kappab through antioxidant effects. Prostaglandins Leukot Essent Fatty Acids. 2002;67(2\u20133):175\u201381.","journal-title":"Prostaglandins Leukot Essent Fatty Acids"},{"issue":"1","key":"1276_CR13","first-page":"42","volume":"12","author":"M Massaro","year":"2002","unstructured":"Massaro M, De R, Caterina. Vasculoprotective effects of oleic acid: epidemiological background and direct vascular antiatherogenic properties. Nutr Metab Cardiovasc Dis. 2002;12(1):42\u201351.","journal-title":"Nutr Metab Cardiovasc Dis"},{"issue":"2","key":"1276_CR14","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1161\/01.ATV.19.2.220","volume":"19","author":"MA Carluccio","year":"1999","unstructured":"Carluccio MA, Massaro M, Bonfrate C, et al. Oleic acid inhibits endothelial activation: a direct vascular antiatherogenic mechanism of a nutritional component in the mediterranean diet. Arterioscler Thromb Vasc Biol. 1999;19(2):220\u20138.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"5","key":"1276_CR15","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1093\/nar\/11.5.1475","volume":"11","author":"JD Dignam","year":"1983","unstructured":"Dignam JD, Lebovitz RM, Roeder RG. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983;11(5):1475\u201389.","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"1276_CR16","doi-asserted-by":"crossref","first-page":"e1315","DOI":"10.1097\/MJT.0000000000000185","volume":"23","author":"IL Baz\u00e1n-Salinas","year":"2016","unstructured":"Baz\u00e1n-Salinas IL, Mat\u00edas-P\u00e9rez D, P\u00e9rez-Campos E, P\u00e9rez-Campos Mayoral L, Garc\u00eda-Montalvo IA. Reduction of platelet aggregation from ingestion of oleic and linoleic acids found in Vitis vinifera and Arachis hypogaea oils. Am J Ther. 2016;23(6):e1315\u20139.","journal-title":"Am J Ther"},{"issue":"5","key":"1276_CR17","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1093\/ajcn\/60.5.717","volume":"60","author":"ME Turini","year":"1994","unstructured":"Turini ME, Powell WS, Behr SR, Holub BJ. Effects of a fish-oil and vegetable-oil formula on aggregation and ethanolamine-containing lysophospholipid generation in activated human platelets and on leukotriene production in stimulated neutrophils. Am J Clin Nutr. 1994;60(5):717\u201324.","journal-title":"Am J Clin Nutr"},{"issue":"15","key":"1276_CR18","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1182\/blood-2014-03-557645","volume":"124","author":"X Fan","year":"2014","unstructured":"Fan X, Shi P, Dai J, et al. Paired immunoglobulin-like receptor B regulates platelet activation. Blood. 2014;124(15):2421\u201330.","journal-title":"Blood"},{"key":"1276_CR19","doi-asserted-by":"crossref","first-page":"11142","DOI":"10.1038\/srep11142","volume":"5","author":"ML Liang","year":"2015","unstructured":"Liang ML, Da XW, He AD, et al. Pentamethylquercetin (PMQ) reduces thrombus formation by inhibiting platelet function. Sci Rep. 2015;5:11142.","journal-title":"Sci Rep"},{"issue":"16","key":"1276_CR20","doi-asserted-by":"crossref","first-page":"4133","DOI":"10.1111\/bph.13191","volume":"172","author":"S Vaiyapuri","year":"2015","unstructured":"Vaiyapuri S, Roweth H, Ali MS, et al. Pharmacological actions of nobiletin in the modulation of platelet function. Br J Pharmacol. 2015;172(16):4133\u201345.","journal-title":"Br J Pharmacol"},{"issue":"4","key":"1276_CR21","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.healun.2020.01.1344","volume":"39","author":"KP Bjerre","year":"2020","unstructured":"Bjerre KP, Clemmensen TS, Berg K, et al. Platelet aggregation and response to aspirin therapy in cardiac allograft vasculopathy. J Heart Lung Transplant. 2020;39(4):371\u20138.","journal-title":"J Heart Lung Transplant"},{"issue":"18","key":"1276_CR22","doi-asserted-by":"crossref","first-page":"3473","DOI":"10.1242\/jcs.187971","volume":"129","author":"D Semeniak","year":"2016","unstructured":"Semeniak D, Kulawig R, Stegner D, et al. Proplatelet formation is selectively inhibited by collagen type I through syk-independent GPVI signaling. J Cell Sci. 2016;129(18):3473\u201384.","journal-title":"J Cell Sci"},{"issue":"2","key":"1276_CR23","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1182\/blood-2002-12-3882","volume":"102","author":"B Nieswandt","year":"2003","unstructured":"Nieswandt B, Watson SP. Platelet-collagen interaction: is GPVI the central receptor? Blood. 2003;102(2):449\u201361.","journal-title":"Blood"},{"issue":"1","key":"1276_CR24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1055\/s-0037-1616217","volume":"86","author":"KJ Clemetson","year":"2001","unstructured":"Clemetson KJ, Clemetson JM. Platelet collagen receptors. Thromb Haemost. 2001;86(1):189\u201397.","journal-title":"Thromb Haemost"},{"issue":"14","key":"1276_CR25","first-page":"1146","volume":"135","author":"E Mammadova-Bach","year":"2020","unstructured":"Mammadova-Bach E, Gil-Pulido J, Sarukhanyan E, et al. Platelet glycoprotein VI promotes metastasis through interaction with cancer cell-derived galectin-3. Blood. 2020;135(14):1146\u201360.","journal-title":"Blood"},{"issue":"1","key":"1276_CR26","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1172\/JCI122955","volume":"129","author":"J Rayes","year":"2019","unstructured":"Rayes J, Watson SP, Nieswandt B. Functional significance of the platelet immune receptors GPVI and CLEC-2. J Clin Invest. 2019;129(1):12\u201323.","journal-title":"J Clin Invest"},{"key":"1276_CR27","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.3389\/fphar.2018.01293","volume":"9","author":"Y Liu","year":"2018","unstructured":"Liu Y, Liu T, Ding K, et al. Phospholipase C\u03b32 signaling cascade contribute to the antiplatelet effect of notoginsenoside fc. Front Pharmacol. 2018;9:1293.","journal-title":"Front Pharmacol"},{"issue":"7","key":"1276_CR28","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1182\/blood-2007-02-075432","volume":"110","author":"A Ragab","year":"2007","unstructured":"Ragab A, S\u00e9verin S, Gratacap MP, et al. Roles of the C-terminal tyrosine residues of LAT in GPVI-induced platelet activation: insights into the mechanism of PLC gamma 2 activation. Blood. 2007;110(7):2466\u201374.","journal-title":"Blood"},{"issue":"1","key":"1276_CR29","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1586\/ehm.09.75","volume":"3","author":"DS Woulfe","year":"2010","unstructured":"Woulfe DS. Akt signaling in platelets and thrombosis. Expert Rev Hematol. 2010;3(1):81\u201391.","journal-title":"Expert Rev Hematol"},{"issue":"6","key":"1276_CR30","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1016\/j.jnutbio.2012.08.007","volume":"24","author":"T Jayakumar","year":"2013","unstructured":"Jayakumar T, Chen WF, Lu WJ, et al. A novel antithrombotic effect of sulforaphane via activation of platelet adenylate cyclase: ex vivo and in vivo studies. J Nutr Biochem. 2013;24(6):1086\u201395.","journal-title":"J Nutr Biochem"},{"issue":"12","key":"1276_CR31","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1182\/bloodadvances.2017015149","volume":"2","author":"X Fan","year":"2018","unstructured":"Fan X, Wang C, Shi P, et al. Platelet MEKK3 regulates arterial thrombosis and myocardial infarct expansion in mice. Blood Adv. 2018;2(12):1439\u201348.","journal-title":"Blood Adv"},{"issue":"12","key":"1276_CR32","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1111\/j.1538-7836.2008.03169.x","volume":"6","author":"F Adam","year":"2008","unstructured":"Adam F, Kauskot A, Rosa JP, Bryckaert M. Mitogen-activated protein kinases in hemostasis and thrombosis. J Thromb Haemost. 2008;6(12):2007\u201316.","journal-title":"J Thromb Haemost"},{"issue":"4","key":"1276_CR33","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0092-8674(00)81892-9","volume":"88","author":"PE Hughes","year":"1997","unstructured":"Hughes PE, Renshaw MW, Pfaff M, et al. Suppression of integrin activation: a novel function of a Ras\/Raf-initiated MAP kinase pathway. Cell. 1997;88(4):521\u201330.","journal-title":"Cell"},{"issue":"6582","key":"1276_CR34","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/381516a0","volume":"381","author":"AJ Connolly","year":"1996","unstructured":"Connolly AJ, Ishihara H, Kahn ML, Farese RV Jr, Coughlin SR. Role of the thrombin receptor in development and evidence for a second receptor. Nature. 1996;381(6582):516\u20139.","journal-title":"Nature"},{"issue":"3","key":"1276_CR35","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1161\/01.ATV.18.3.404","volume":"18","author":"I Hers","year":"1998","unstructured":"Hers I, Donath J, van Willigen G, Akkerman JW. Differential involvement of tyrosine and serine\/threonine kinases in platelet integrin alphaIIbbeta3 exposure. Arterioscler Thromb Vasc Biol. 1998;18(3):404\u201314.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"6","key":"1276_CR36","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.3390\/nu13061740","volume":"13","author":"Y Pang","year":"2021","unstructured":"Pang Y, Xu X, Xiang X, et al. High Fat activates O-GlcNAcylation and affects AMPK\/ACC pathway to regulate lipid metabolism. Nutrients. 2021;13(6):1740.","journal-title":"Nutrients"},{"issue":"12","key":"1276_CR37","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1182\/blood-2010-04-279612","volume":"116","author":"V Randriamboavonjy","year":"2010","unstructured":"Randriamboavonjy V, Isaak J, Fr\u00f6mel T, et al. AMPK \u03b12 subunit is involved in platelet signaling, clot retraction, and thrombus stability. Blood. 2010;116(12):2134\u201340.","journal-title":"Blood"},{"issue":"12","key":"1276_CR38","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1038\/nm.3372","volume":"19","author":"MD Fullerton","year":"2013","unstructured":"Fullerton MD, Galic S, Marcinko K, et al. Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin-sensitizing effects of metformin. Nat Med. 2013;19(12):1649\u201354.","journal-title":"Nat Med"},{"issue":"6","key":"1276_CR39","doi-asserted-by":"crossref","first-page":"C765","DOI":"10.1152\/ajpcell.00262.2016","volume":"312","author":"S Geue","year":"2017","unstructured":"Geue S, Walker-Allgaier B, Ei\u00dfler D, et al. Doxepin inhibits GPVI-dependent platelet ca(2+) signaling and collagen-dependent thrombus formation. Am J Physiol Cell Physiol. 2017;312(6):C765\u2013c774.","journal-title":"Am J Physiol Cell Physiol"},{"issue":"6","key":"1276_CR40","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.molcel.2017.05.032","volume":"66","author":"D Garcia","year":"2017","unstructured":"Garcia D, Shaw RJ. AMPK: mechanisms of cellular energy sensing and restoration of metabolic balance. Mol Cell. 2017;66(6):789\u2013800.","journal-title":"Mol Cell"},{"issue":"16","key":"1276_CR41","doi-asserted-by":"crossref","first-page":"5933","DOI":"10.1128\/MCB.00383-06","volume":"26","author":"N Stahmann","year":"2006","unstructured":"Stahmann N, Woods A, Carling D, Heller R. Thrombin activates AMP-activated protein kinase in endothelial cells via a pathway involving Ca2+\/calmodulin-dependent protein kinase kinase beta. Mol Cell Biol. 2006;26(16):5933\u201345.","journal-title":"Mol Cell Biol"},{"issue":"6","key":"1276_CR42","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1111\/jth.12568","volume":"12","author":"MB Onselaer","year":"2014","unstructured":"Onselaer MB, Oury C, Hunter RW, et al. The ca(2+) \/calmodulin-dependent kinase kinase \u03b2-AMP-activated protein kinase-\u03b11 pathway regulates phosphorylation of cytoskeletal targets in thrombin-stimulated human platelets. J Thromb Haemost. 2014;12(6):973\u201386.","journal-title":"J Thromb Haemost"},{"issue":"2","key":"1276_CR43","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1182\/blood-2004-11-4377","volume":"107","author":"D Pandey","year":"2006","unstructured":"Pandey D, Goyal P, Bamburg JR, Siess W. Regulation of LIM-kinase 1 and cofilin in thrombin-stimulated platelets. Blood. 2006;107(2):575\u201383.","journal-title":"Blood"},{"issue":"13","key":"1276_CR44","doi-asserted-by":"crossref","first-page":"4035","DOI":"10.1182\/blood-2006-05-023739","volume":"108","author":"G Pula","year":"2006","unstructured":"Pula G, Schuh K, Nakayama K, Nakayama KI, Walter U, Poole AW. PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation. Blood. 2006;108(13):4035\u201344.","journal-title":"Blood"},{"key":"1276_CR45","doi-asserted-by":"crossref","first-page":"e06126","DOI":"10.7554\/eLife.06126","volume":"4","author":"S Tojkander","year":"2015","unstructured":"Tojkander S, Gateva G, Husain A, Krishnan R, Lappalainen P. Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly. Elife. 2015;4:e06126.","journal-title":"Elife"},{"issue":"6","key":"1276_CR46","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.cmet.2014.09.013","volume":"20","author":"DG Hardie","year":"2014","unstructured":"Hardie DG. AMPK\u2013sensing energy while talking to other signaling pathways. Cell Metab. 2014;20(6):939\u201352.","journal-title":"Cell Metab"},{"key":"1276_CR47","doi-asserted-by":"crossref","first-page":"841187","DOI":"10.3389\/fnut.2022.841187","volume":"9","author":"X Liu","year":"2022","unstructured":"Liu X, Zhao T, Wei X, Zhang D, Lv W, Luo Z. Dietary phosphorus reduced hepatic lipid deposition by activating Ampk Pathway and Beclin1 phosphorylation levels to activate Lipophagy in Tilapia Oreochromis niloticus. Front Nutr. 2022;9:841187.","journal-title":"Front Nutr"},{"issue":"11","key":"1276_CR48","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1182\/blood-2018-02-831503","volume":"132","author":"S Lepropre","year":"2018","unstructured":"Lepropre S, Kautbally S, Octave M, et al. AMPK-ACC signaling modulates platelet phospholipids and potentiates thrombus formation. Blood. 2018;132(11):1180\u201392.","journal-title":"Blood"},{"issue":"8","key":"1276_CR49","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/S0006-2952(00)00417-2","volume":"60","author":"B Payrastre","year":"2000","unstructured":"Payrastre B, Missy K, Trumel C, Bodin S, Plantavid M, Chap H. The integrin alpha IIb\/beta 3 in human platelet signal transduction. Biochem Pharmacol. 2000;60(8):1069\u201374.","journal-title":"Biochem Pharmacol"},{"issue":"1","key":"1276_CR50","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1182\/blood.V70.1.307.307","volume":"70","author":"SJ Shattil","year":"1987","unstructured":"Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 1987;70(1):307\u201315.","journal-title":"Blood"},{"issue":"14","key":"1276_CR51","doi-asserted-by":"crossref","first-page":"3352","DOI":"10.1182\/blood-2011-12-397398","volume":"119","author":"MU Naik","year":"2012","unstructured":"Naik MU, Stalker TJ, Brass LF, Naik UP. JAM-A protects from thrombosis by suppressing integrin \u03b1IIb\u03b23-dependent outside-in signaling in platelets. Blood. 2012;119(14):3352\u201360.","journal-title":"Blood"}],"container-title":["Cell Communication and Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-023-01276-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12964-023-01276-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-023-01276-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T14:15:46Z","timestamp":1730297746000},"score":1,"resource":{"primary":{"URL":"https:\/\/biosignaling.biomedcentral.com\/articles\/10.1186\/s12964-023-01276-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,10]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["1276"],"URL":"https:\/\/doi.org\/10.1186\/s12964-023-01276-0","relation":{},"ISSN":["1478-811X"],"issn-type":[{"value":"1478-811X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,10]]},"assertion":[{"value":"6 May 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 August 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 October 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were agreed by the Institutional Animal Care and Utilization Committee of Tongji Medical College, Huazhong University of Science and Technology (2020-S2324). The human experiment was approved by the Medical Ethics Committee of Tongji Medical College and Union Hospital of Huazhong University of Science and Technology (2020\u2009\u2212\u20090245). The study was consistent with the criteria of the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"278"}}