{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:29:21Z","timestamp":1791689361077,"version":"4.3.4"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201249"],"award-info":[{"award-number":["82201249"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770981"],"award-info":[{"award-number":["81770981"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The medical and health research project of Zhejiang province","award":["2023KY172"],"award-info":[{"award-number":["2023KY172"]}]},{"name":"The Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University","award":["JCRCGW-2022-005"],"award-info":[{"award-number":["JCRCGW-2022-005"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pyroptosis is crucial for controlling various immune cells. However, the role of allergen-induced CD11c\u2009+\u2009dendritic cell (DC) pyroptosis in allergic rhinitis (AR) remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mice were grouped into the control group, AR group and necrosulfonamide-treated AR group (AR\u2009+\u2009NSA group). The allergic symptom scores, OVA-sIgE titres, serum IL-1\u03b2\/IL-18 levels, histopathological characteristics and T-helper cell-related cytokines were evaluated. CD11c\/GSDMD-N-positive cells were examined by immunofluorescence analysis. Murine CD11c\u2009+\u2009bone marrow-derived DCs (BMDCs) were induced in vitro, stimulated with OVA\/HDM, treated with necrosulfonamide (NSA), and further cocultured with lymphocytes to assess BMDC function. An adoptive transfer murine model was used to study the role of BMDC pyroptosis in allergic rhinitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Inhibiting GSDMD-N-mediated pyroptosis markedly protected against Th1\/Th2\/Th17 imbalance and alleviated inflammatory responses in the AR model. GSDMD-N was mainly coexpressed with CD11c (a DC marker) in AR mice. In vitro, OVA\/HDM stimulation increased pyroptotic morphological abnormalities and increased the expression of pyroptosis-related proteins in a dose-dependent manner; moreover, inhibiting pyroptosis significantly decreased pyroptotic morphology and NLRP3, C-Caspase1 and GSDMD-N expression. In addition, OVA-induced BMDC pyroptosis affected CD4\u2009+\u2009T-cell differentiation and related cytokine levels, leading to Th1\/Th2\/Th17 cell imbalance. However, the Th1\/Th2\/Th17 cell immune imbalance was significantly reversed by NSA. Adoptive transfer of OVA-loaded BMDCs promoted allergic inflammation, while the administration of NSA to OVA-loaded BMDCs significantly reduced AR inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Allergen-induced dendritic cell pyroptosis promotes the development of allergic rhinitis through GSDMD-N-mediated pyroptosis, which provides a clue to allergic disease interventions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12964-023-01309-8","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T13:02:00Z","timestamp":1696942920000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Allergen-induced CD11c\u2009+\u2009dendritic cell pyroptosis aggravates allergic rhinitis"],"prefix":"10.1186","volume":"21","author":[{"given":"Yue-Long","family":"Qiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Wan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wo-Er","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Feng","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze-Zhang","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Ming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,10]]},"reference":[{"key":"1309_CR1","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.semcdb.2018.02.014","volume":"86","author":"S Alculumbre","year":"2019","unstructured":"Alculumbre S, Raieli S, Hoffmann C, et al. Plasmacytoid pre-dendritic cells (pDC): from molecular pathways to function and disease association. Semin Cell Dev Biol. 2019;86:24\u201335.","journal-title":"Semin Cell Dev Biol"},{"key":"1309_CR2","doi-asserted-by":"publisher","first-page":"343","DOI":"10.2147\/JIR.S254717","volume":"13","author":"S Feng","year":"2020","unstructured":"Feng S, Ju L, Shao Z, Grzanna M, et al. Therapeutic effect of C-C chemokine receptor type 1 (CCR1) antagonist BX471 on allergic Rhinitis. J Inflamm Res. 2020;13:343\u201356.","journal-title":"J Inflamm Res"},{"issue":"3","key":"1309_CR3","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1038\/s41418-017-0012-4","volume":"25","author":"L Galluzzi","year":"2018","unstructured":"Galluzzi L, Vitale I, Aaronson SA, et al. Molecular mechanisms of cell death: recommendations of the nomenclature Committee on Cell Death 2018. Cell Death Differ. 2018;25(3):486\u2013541.","journal-title":"Cell Death Differ"},{"issue":"2","key":"1309_CR4","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/cmi.2010.81","volume":"8","author":"C Conforti-Andreoni","year":"2011","unstructured":"Conforti-Andreoni C, Ricciardi-Castagnoli P, Mortellaro A. The inflammasomes in health and disease: from genetics to molecular mechanisms of autoinflammation and beyond. Cell Mol Immunol. 2011;8(2):135\u201345.","journal-title":"Cell Mol Immunol"},{"key":"1309_CR5","doi-asserted-by":"publisher","first-page":"3209","DOI":"10.1038\/ncomms4209","volume":"5","author":"N Van Opdenbosch","year":"2014","unstructured":"Van Opdenbosch N, Gurung P, Vande Walle L, et al. Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation. Nat Commun. 2014;5:3209.","journal-title":"Nat Commun"},{"issue":"7575","key":"1309_CR6","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/nature15541","volume":"526","author":"N Kayagaki","year":"2015","unstructured":"Kayagaki N, Stowe IB, Lee BL, et al. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling. Nature. 2015;526(7575):666\u201371.","journal-title":"Nature"},{"key":"1309_CR7","doi-asserted-by":"publisher","first-page":"2187","DOI":"10.2147\/JIR.S291453","volume":"14","author":"AA Mamun","year":"2021","unstructured":"Mamun AA, Wu Y, Nasrin F, et al. Role of pyroptosis in diabetes and its therapeutic implications. J Inflamm Res. 2021;14:2187\u2013206.","journal-title":"J Inflamm Res"},{"issue":"7575","key":"1309_CR8","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1038\/nature15514","volume":"526","author":"J Shi","year":"2015","unstructured":"Shi J, Zhao Y, Wang K, et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature. 2015;526(7575):660\u20135.","journal-title":"Nature"},{"issue":"28","key":"1309_CR9","doi-asserted-by":"publisher","first-page":"7858","DOI":"10.1073\/pnas.1607769113","volume":"113","author":"RA Aglietti","year":"2016","unstructured":"Aglietti RA, Estevez A, Gupta A, et al. GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes. Proc Natl Acad Sci U S A. 2016;113(28):7858\u201363.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1309_CR10","doi-asserted-by":"publisher","first-page":"109925","DOI":"10.1016\/j.biopha.2020.109925","volume":"125","author":"J Zhuang","year":"2020","unstructured":"Zhuang J, Cui H, Zhuang L, et al. Bronchial epithelial pyroptosis promotes airway inflammation in a murine model of toluene diisocyanate-induced asthma. Biomed Pharmacother. 2020;125:109925.","journal-title":"Biomed Pharmacother"},{"issue":"1","key":"1309_CR11","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.bbrc.2019.11.031","volume":"522","author":"Z Yang","year":"2020","unstructured":"Yang Z, Liang C, Wang T, et al. NLRP3 inflammasome activation promotes the development of allergic rhinitis via epithelium pyroptosis. Biochem Biophys Res Commun. 2020;522(1):61\u20137.","journal-title":"Biochem Biophys Res Commun"},{"issue":"6673","key":"1309_CR12","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1038\/32588","volume":"392","author":"J Banchereau","year":"1998","unstructured":"Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. 1998;392(6673):245\u201352.","journal-title":"Nature"},{"issue":"4","key":"1309_CR13","first-page":"2665","volume":"20","author":"B Bodey","year":"2000","unstructured":"Bodey B, Bodey B Jr, Siegel SE, et al. Failure of cancer vaccines: the significant limitations of this approach to immunotherapy. Anticancer Res. 2000;20(4):2665\u201376.","journal-title":"Anticancer Res"},{"issue":"26","key":"1309_CR14","doi-asserted-by":"publisher","first-page":"eaat2738","DOI":"10.1126\/sciimmunol.aat2738","volume":"3","author":"JK Rathkey","year":"2018","unstructured":"Rathkey JK, Zhao J, Liu Z, et al. Chemical disruption of the pyroptotic pore-forming protein gasdermin D inhibits inflammatory cell death and sepsis. Sci Immunol. 2018;3(26):eaat2738.","journal-title":"Sci Immunol"},{"issue":"3","key":"1309_CR15","doi-asserted-by":"publisher","first-page":"736","DOI":"10.4049\/jimmunol.1900228","volume":"203","author":"M Rashidi","year":"2019","unstructured":"Rashidi M, Simpson DS, Hempel A, et al. The pyroptotic cell death Effector Gasdermin D is activated by Gout-Associated Uric acid crystals but is dispensable for cell death and IL-1\u03b2 release. J Immunol. 2019;203(3):736\u201348.","journal-title":"J Immunol"},{"issue":"9809","key":"1309_CR16","doi-asserted-by":"publisher","first-page":"2112","DOI":"10.1016\/S0140-6736(11)60130-X","volume":"378","author":"AN Greiner","year":"2011","unstructured":"Greiner AN, Hellings PW, Rotiroti G, et al. Allergic rhinitis. Lancet. 2011;378(9809):2112\u201322.","journal-title":"Lancet"},{"issue":"1","key":"1309_CR17","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1038\/s42003-020-01466-3","volume":"3","author":"N Miyanaga","year":"2020","unstructured":"Miyanaga N, Takagi H, Uto T, et al. Essential role of submandibular lymph node dendritic cells in protective sublingual immunotherapy against murine allergy. Commun Biol. 2020;3(1):742.","journal-title":"Commun Biol"},{"issue":"1","key":"1309_CR18","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1159\/000493328","volume":"178","author":"WE Jiao","year":"2019","unstructured":"Jiao WE, Wei JF, Kong YG, et al. Notch Signaling promotes development of allergic rhinitis by suppressing Foxp3 expression and Treg Cell differentiation. Int Arch Allergy Immunol. 2019;178(1):33\u201344.","journal-title":"Int Arch Allergy Immunol"},{"key":"1309_CR19","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/j.intimp.2019.04.039","volume":"72","author":"S Xu","year":"2019","unstructured":"Xu S, Kong YG, Jiao WE, et al. Tangeretin promotes regulatory T cell differentiation by inhibiting Notch1\/Jagged1 signaling in allergic rhinitis. Int Immunopharmacol. 2019;72:402\u201312.","journal-title":"Int Immunopharmacol"},{"issue":"9","key":"1309_CR20","doi-asserted-by":"publisher","first-page":"5184","DOI":"10.4049\/jimmunol.1002683","volume":"186","author":"P Sathe","year":"2011","unstructured":"Sathe P, Pooley J, Vremec D, et al. The acquisition of antigen cross-presentation function by newly formed dendritic cells. J Immunol. 2011;186(9):5184\u201392.","journal-title":"J Immunol"},{"issue":"1","key":"1309_CR21","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1186\/s13071-019-3411-1","volume":"12","author":"N Wei","year":"2019","unstructured":"Wei N, Lin Z, Xu Z, et al. Immunosuppressive effects of tick protein RHcyst-1 on murine bone marrow-derived dendritic cells. Parasit Vectors. 2019;12(1):169.","journal-title":"Parasit Vectors"},{"issue":"1","key":"1309_CR22","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1186\/s13046-021-02191-3","volume":"40","author":"CL Qi","year":"2021","unstructured":"Qi CL, Huang ML, Zou Y, et al. The IRF2\/CENP-N\/AKT signaling axis promotes proliferation, cell cycling and apoptosis resistance in nasopharyngeal carcinoma cells by increasing aerobic glycolysis. J Exp Clin Cancer Res. 2021;40(1):390.","journal-title":"J Exp Clin Cancer Res"},{"issue":"2","key":"1309_CR23","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.jaci.2022.02.031","volume":"150","author":"Y Li","year":"2022","unstructured":"Li Y, Chang LH, Huang WQ, et al. IL-17A mediates pyroptosis via the ERK pathway and contributes to steroid resistance in CRSwNP. J Allergy Clin Immunol. 2022;150(2):337\u201351.","journal-title":"J Allergy Clin Immunol"},{"issue":"4","key":"1309_CR24","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1111\/j.1365-2567.2009.03142.x","volume":"128","author":"LC van den Berk","year":"2009","unstructured":"van den Berk LC, Roelofs H, Huijs T, et al. Cord blood mesenchymal stem cells propel human dendritic cells to an intermediate maturation state and boost interleukin-12 production by mature dendritic cells. Immunology. 2009;128(4):564\u201372.","journal-title":"Immunology"},{"issue":"7","key":"1309_CR25","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1038\/ni.1738","volume":"10","author":"CL Sokol","year":"2009","unstructured":"Sokol CL, Chu NQ, Yu S, et al. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat Immunol. 2009;10(7):713\u201320.","journal-title":"Nat Immunol"},{"key":"1309_CR26","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.clim.2016.11.001","volume":"174","author":"M Berker","year":"2017","unstructured":"Berker M, Frank LJ, Ge\u00dfner AL, et al. Allergies - A T cells perspective in the era beyond the TH1\/TH2 paradigm. Clin Immunol. 2017;174:73\u201383.","journal-title":"Clin Immunol"},{"key":"1309_CR27","doi-asserted-by":"crossref","unstructured":"Wang Y, Cui X, Yuan L, et al. A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1\/Th2 Immune Responses in Mice. J Immunol Res. 2020;2020:2714257.","DOI":"10.1155\/2020\/2714257"},{"issue":"1","key":"1309_CR28","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/s40425-019-0517-0","volume":"7","author":"AS Laino","year":"2019","unstructured":"Laino AS, Betts BC, Veerapathran A, et al. HDAC6 selective inhibition of melanoma patient T-cells augments anti-tumor characteristics. J Immunother Cancer. 2019;7(1):33.","journal-title":"J Immunother Cancer"},{"issue":"5","key":"1309_CR29","doi-asserted-by":"publisher","first-page":"2701","DOI":"10.4049\/jimmunol.164.5.2701","volume":"164","author":"JS Wild","year":"2000","unstructured":"Wild JS, Sigounas A, Sur N, et al. IFN-gamma-inducing factor (IL-18) increases allergic sensitization, serum IgE, Th2 cytokines, and airway eosinophilia in a mouse model of allergic asthma. J Immunol. 2000;164(5):2701\u201310.","journal-title":"J Immunol"},{"issue":"8","key":"1309_CR30","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1093\/intimm\/8.8.1257","volume":"8","author":"M Huber","year":"1996","unstructured":"Huber M, Rutherford A, Meister W, et al. TCR- and IL-1-mediated co-stimulation reveals an IL-4-independent way of Th2 cell proliferation. Int Immunol. 1996;8(8):1257\u201363.","journal-title":"Int Immunol"},{"issue":"4","key":"1309_CR31","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1684\/ecn.2018.0401","volume":"28","author":"M Esmailbeig","year":"2017","unstructured":"Esmailbeig M, Ghaderi A. Interleukin-18: a regulator of cancer and autoimmune diseases. Eur Cytokine Netw. 2017;28(4):127\u201340.","journal-title":"Eur Cytokine Netw"},{"issue":"46","key":"1309_CR32","doi-asserted-by":"publisher","first-page":"19961","DOI":"10.1073\/pnas.1014465107","volume":"107","author":"NT Nguyen","year":"2010","unstructured":"Nguyen NT, Kimura A, Nakahama T, et al. Aryl hydrocarbon receptor negatively regulates dendritic cell immunogenicity via a kynurenine-dependent mechanism. Proc Natl Acad Sci U S A. 2010;107(46):19961\u20136.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"1309_CR33","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1038\/77811","volume":"1","author":"T Yoshimoto","year":"2000","unstructured":"Yoshimoto T, Mizutani H, Tsutsui H, et al. IL-18 induction of IgE: dependence on CD4 + T cells, IL-4 and STAT6. Nat Immunol. 2000;1(2):132\u20137.","journal-title":"Nat Immunol"},{"issue":"10","key":"1309_CR34","doi-asserted-by":"publisher","first-page":"5738","DOI":"10.4049\/jimmunol.1003597","volume":"186","author":"SJ Lalor","year":"2011","unstructured":"Lalor SJ, Dungan LS, Sutton CE, et al. Caspase-1-processed cytokines IL-1beta and IL-18 promote IL-17 production by gammadelta and CD4 T cells that mediate autoimmunity. J Immunol. 2011;186(10):5738\u201348.","journal-title":"J Immunol"},{"key":"1309_CR35","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1073\/pnas.2117636119","volume":"119","author":"S Kim","year":"2022","unstructured":"Kim S, Lu HC, Steelman AJ, et al. Myeloid caspase-8 restricts RIPK3-dependent proinflammatory IL-1\u03b2 production and CD4 T cell activation in autoimmune demyelination. Proc Natl Acad Sci U S A. 2022;119:24.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2\u20133","key":"1309_CR36","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1007\/s12031-023-02104-3","volume":"73","author":"Y Guan","year":"2023","unstructured":"Guan Y, Xu B, Sui Y, et al. Cytohesin-4 Upregulation in Glioma-Associated M2 macrophages is correlated with pyroptosis and poor prognosis. J Mol Neurosci. 2023;73(2\u20133):143\u201358.","journal-title":"J Mol Neurosci"},{"issue":"7918","key":"1309_CR37","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1038\/s41586-022-04801-2","volume":"607","author":"A Kalbasi","year":"2022","unstructured":"Kalbasi A, Siurala M, Su LL, et al. Potentiating adoptive cell therapy using synthetic IL-9 receptors. Nature. 2022;607(7918):360\u20135.","journal-title":"Nature"},{"issue":"7","key":"1309_CR38","doi-asserted-by":"publisher","first-page":"1421","DOI":"10.1038\/s41591-022-01799-y","volume":"28","author":"Y Zhang","year":"2022","unstructured":"Zhang Y, Vu T, Palmer DC, et al. A T cell resilience model associated with response to immunotherapy in multiple tumor types. Nat Med. 2022;28(7):1421\u201331.","journal-title":"Nat Med"}],"container-title":["Cell Communication and Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-023-01309-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12964-023-01309-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-023-01309-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:18:36Z","timestamp":1700551116000},"score":1,"resource":{"primary":{"URL":"https:\/\/biosignaling.biomedcentral.com\/articles\/10.1186\/s12964-023-01309-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,10]]},"references-count":38,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["1309"],"URL":"https:\/\/doi.org\/10.1186\/s12964-023-01309-8","relation":{},"ISSN":["1478-811X"],"issn-type":[{"value":"1478-811X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,10]]},"assertion":[{"value":"25 April 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 October 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments described herein were approved by the Institutional Animal Care and Use Committee of Renmin Hospital of Wuhan University (licence no.: WDRM-20190509).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"281"}}