{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T10:24:49Z","timestamp":1794479089428,"version":"4.9.1"},"reference-count":72,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["UMO-2022\/45\/N\/\/NZ6\/01454"],"award-info":[{"award-number":["UMO-2022\/45\/N\/\/NZ6\/01454"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","award":["FNP START 075\/2024"],"award-info":[{"award-number":["FNP START 075\/2024"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005297","name":"Uniwersytet Medyczny w Bialymstoku","doi-asserted-by":"publisher","award":["B.SUB.23.326"],"award-info":[{"award-number":["B.SUB.23.326"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vimentin is a type III intermediate filament protein that plays an important role in cytoskeletal mechanics. It is now known that vimentin also has distinct functions outside the cell. Recent studies show the controlled release of vimentin into the extracellular environment, where it functions as a signaling molecule. Such observations are expanding our current knowledge of vimentin as a structural cellular component towards additional roles as an active participant in cell signaling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Our study investigates the immunological roles of extracellular vimentin (eVim) and its citrullinated form (CitVim) as a damage-associated molecular pattern (DAMP) engaging the Toll-like receptor 4 (TLR4) of human neutrophils. We used in vitro assays to study neutrophil migration through endothelial cell monolayers and activation markers such as NADPH oxidase subunit 2 (NOX2\/gp91phox). The comparison of eVim with CitVim and its effect on human neutrophils was extended to the induction of extracellular traps (NETs) and phagocytosis of pathogens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both eVim and CitVim interact with and trigger TLR4, leading to increased neutrophil migration and adhesion. CitVim stimulated the enhanced migratory ability of neutrophils, activation of NF-\u03baB, and induction of NET formation mainly mediated through reactive oxygen species (ROS)-dependent and TLR4-dependent pathways. In contrast, neutrophils exposed to non-citrullinated vimentin exhibited higher efficiency in favoring pathogen phagocytosis, such as\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , compared to CitVim.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study identifies new functions of eVim in its native and modified forms as an extracellular matrix DAMP and highlights its importance in the modulation of immune system functions. The differential effects of eVim and CitVim on neutrophil functions highlight their potential as new molecular targets for therapeutic strategies aimed at regulation of neutrophil activity in different pathological conditions. This, in turn, opens new windows of therapeutic intervention in inflammatory and immunological diseases characterized by immune system dysfunction, in which eVim and CitVim play a key role.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12964-025-02062-w","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T09:45:31Z","timestamp":1738748731000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Extracellular vimentin is a damage-associated molecular pattern protein serving as an agonist of TLR4 in human neutrophils"],"prefix":"10.1186","volume":"23","author":[{"given":"\u0141ukasz","family":"Suprewicz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Fiedoruk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karol","family":"Sk\u0142odowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Hutt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Zakrzewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Walewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Deptu\u0142a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Lesiak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u0142awomir","family":"Ok\u0142a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Galie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison E.","family":"Patteson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Janmey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bucki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"2062_CR1","doi-asserted-by":"publisher","first-page":"113","DOI":"10.3389\/fphys.2018.00113","volume":"9","author":"C Rosales","year":"2018","unstructured":"Rosales C. Neutrophil: a cell with many roles in inflammation or several cell types? Front Physiol. 2018;9:113.","journal-title":"Front Physiol"},{"issue":"178","key":"2062_CR2","doi-asserted-by":"publisher","first-page":"ra17840","DOI":"10.1126\/scitranslmed.3005580","volume":"5","author":"R Khandpur","year":"2013","unstructured":"Khandpur R, Carmona-Rivera C, Vivekanandan-Giri A, Gizinski A, Yalavarthi S, Knight JS, et al. NETs are a source of citrullinated autoantigens and stimulate inflammatory responses in rheumatoid arthritis. Sci Transl Med. 2013;5(178):ra17840\u201340.","journal-title":"Sci Transl Med"},{"issue":"4","key":"2062_CR3","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1159\/000347132","volume":"5","author":"K Pittman","year":"2013","unstructured":"Pittman K, Kubes P. Damage-associated molecular patterns control neutrophil recruitment. J Innate Immun. 2013;5(4):315\u201323.","journal-title":"J Innate Immun"},{"key":"2062_CR4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.expneurol.2013.11.020","volume":"258","author":"AD Gaudet","year":"2014","unstructured":"Gaudet AD, Popovich PG. Extracellular matrix regulation of inflammation in the healthy and injured spinal cord. Exp Neurol. 2014;258:24\u201334.","journal-title":"Exp Neurol"},{"issue":"1","key":"2062_CR5","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1038\/s41392-021-00687-0","volume":"6","author":"D Li","year":"2021","unstructured":"Li D, Wu M. Pattern recognition receptors in health and diseases. Signal Transduct Target Therapy. 2021;6(1):291.","journal-title":"Signal Transduct Target Therapy"},{"key":"2062_CR6","doi-asserted-by":"publisher","first-page":"927193","DOI":"10.3389\/fcimb.2022.927193","volume":"12","author":"X Zhou","year":"2022","unstructured":"Zhou X, Jin S, Pan J, Lin Q, Yang S, Ambe PC, et al. Damage associated molecular patterns and neutrophil extracellular traps in acute pancreatitis. Front Cell Infect Microbiol. 2022;12:927193.","journal-title":"Front Cell Infect Microbiol"},{"issue":"2","key":"2062_CR7","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1002\/hep.27841","volume":"62","author":"H Huang","year":"2015","unstructured":"Huang H, Tohme S, Al-Khafaji AB, Tai S, Loughran P, Chen L, et al. Damage\u2010associated molecular pattern\u2013activated neutrophil extracellular trap exacerbates sterile inflammatory liver injury. Hepatology. 2015;62(2):600\u201314.","journal-title":"Hepatology"},{"issue":"46","key":"2062_CR8","doi-asserted-by":"publisher","first-page":"5931","DOI":"10.1038\/onc.2016.104","volume":"35","author":"C Hernandez","year":"2016","unstructured":"Hernandez C, Huebener P, Schwabe RF. Damage-associated molecular patterns in cancer: a double-edged sword. Oncogene. 2016;35(46):5931\u201341.","journal-title":"Oncogene"},{"key":"2062_CR9","doi-asserted-by":"crossref","unstructured":"Pogoda K, Byfield F, Deptu\u0142a P, Cie\u015bluk M, Suprewicz \u0141, Sk\u0142odowski K et al. Unique role of Vimentin Networks in Compression Stiffening of cells and protection of nuclei from compressive stress. Nano Lett. 2022.","DOI":"10.1021\/acs.nanolett.2c00736"},{"issue":"7\u20138","key":"2062_CR10","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1101\/gad.349358.122","volume":"36","author":"KM Ridge","year":"2022","unstructured":"Ridge KM, Eriksson JE, Pekny M, Goldman RD. Roles of vimentin in health and disease. Genes Dev. 2022;36(7\u20138):391\u2013407.","journal-title":"Genes Dev"},{"issue":"2","key":"2062_CR11","first-page":"68","volume":"15","author":"N Sadeghi","year":"2023","unstructured":"Sadeghi N, Fazli G, Bayat AA, Fatemi R, Ebrahimnejhad N, Salimi A, et al. Cell surface vimentin detection in Cancer cells by peptide-based monoclonal antibody. Avicenna J Med Biotechnol. 2023;15(2):68.","journal-title":"Avicenna J Med Biotechnol"},{"key":"2062_CR12","doi-asserted-by":"crossref","unstructured":"Amraei R, Xia C, Olejnik J, White MR, Napoleon MA, Lotfollahzadeh S et al. Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells. Proceedings of the National Academy of Sciences. 2022;119(6).","DOI":"10.1073\/pnas.2113874119"},{"issue":"13","key":"2062_CR13","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1091\/mbc.E17-06-0364","volume":"29","author":"J Walker","year":"2018","unstructured":"Walker J, Bleaken B, Romisher A, Alnwibit A, Menko A. In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype. Mol Biol Cell. 2018;29(13):1555\u201370.","journal-title":"Mol Biol Cell"},{"issue":"585","key":"2062_CR14","doi-asserted-by":"publisher","first-page":"eaba2927","DOI":"10.1126\/scitranslmed.aba2927","volume":"13","author":"FJ Li","year":"2021","unstructured":"Li FJ, Surolia R, Li H, Wang Z, Liu G, Kulkarni T, et al. Citrullinated vimentin mediates development and progression of lung fibrosis. Sci Transl Med. 2021;13(585):eaba2927.","journal-title":"Sci Transl Med"},{"key":"2062_CR15","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.ijid.2022.05.018","volume":"121","author":"H Miao","year":"2022","unstructured":"Miao H, Cui Y, Lu Y, Sun T, Dou J, Ren Y, et al. Serum vimentin predicts mortality in pediatric severe sepsis: a prospective observational study. Int J Infect Dis. 2022;121:141\u20137.","journal-title":"Int J Infect Dis"},{"issue":"5","key":"2062_CR16","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1093\/rheumatology\/keq419","volume":"50","author":"K Van Steendam","year":"2011","unstructured":"Van Steendam K, Tilleman K, Deforce D. The relevance of citrullinated vimentin in the production of antibodies against citrullinated proteins and the pathogenesis of rheumatoid arthritis. Rheumatology. 2011;50(5):830\u20137.","journal-title":"Rheumatology"},{"issue":"700\u2013710","key":"2062_CR17","first-page":"568","volume":"16","author":"E Witalison","year":"2015","unstructured":"Witalison E, Thompson P, Hofseth L. Protein arginine deiminases and Associated 566 citrullination: physiological functions and diseases Associated with Dysregulation. Curr 567 Drug Targets. 2015;16(700\u2013710):568.","journal-title":"Curr 567 Drug Targets"},{"issue":"8","key":"2062_CR18","doi-asserted-by":"publisher","first-page":"2503","DOI":"10.1002\/art.22817","volume":"56","author":"H Bang","year":"2007","unstructured":"Bang H, Egerer K, Gauliard A, L\u00fcthke K, Rudolph PE, Fredenhagen G, et al. Mutation and citrullination modifies vimentin to a novel autoantigen for rheumatoid arthritis. Arthr Rhuem. 2007;56(8):2503\u201311.","journal-title":"Arthr Rhuem"},{"key":"2062_CR19","doi-asserted-by":"crossref","unstructured":"Thiam HR, Wong SL, Qiu R, Kittisopikul M, Vahabikashi A, Goldman AE et al. NETosis proceeds by cytoskeleton and endomembrane disassembly and PAD4-mediated chromatin decondensation and nuclear envelope rupture. Proceedings of the National Academy of Sciences. 2020;117(13):7326-37.","DOI":"10.1073\/pnas.1909546117"},{"issue":"1","key":"2062_CR20","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1097\/BOR.0000000000000452","volume":"30","author":"E Darrah","year":"2018","unstructured":"Darrah E, Andrade F. Rheumatoid arthritis and citrullination. Curr Opin Rheumatol. 2018;30(1):72.","journal-title":"Curr Opin Rheumatol"},{"issue":"6","key":"2062_CR21","doi-asserted-by":"publisher","first-page":"2105640","DOI":"10.1002\/smll.202105640","volume":"18","author":"\u0141 Suprewicz","year":"2022","unstructured":"Suprewicz \u0141, Swoger M, Gupta S, Piktel E, Byfield FJ, Iwamoto DV, et al. Extracellular Vimentin as a target against SARS-CoV\u20102 host cell Invasion. Small. 2022;18(6):2105640.","journal-title":"Small"},{"issue":"3","key":"2062_CR22","doi-asserted-by":"publisher","first-page":"2870","DOI":"10.3390\/cancers3032870","volume":"3","author":"NF Steinmetz","year":"2011","unstructured":"Steinmetz NF, Maurer J, Sheng H, Bensussan A, Maricic I, Kumar V, et al. Two domains of vimentin are expressed on the surface of lymph node, bone and brain metastatic prostate cancer lines along with the putative stem cell marker proteins CD44 and CD133. Cancers. 2011;3(3):2870\u201385.","journal-title":"Cancers"},{"key":"2062_CR23","doi-asserted-by":"crossref","unstructured":"Bucki R, Iwamoto D, Shi X, Kerr KE, Byfield FJ, Suprewicz L et al. Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures. bioRxiv. 2022:2022.11. 28.518249.","DOI":"10.1101\/2022.11.28.518249"},{"key":"2062_CR24","doi-asserted-by":"crossref","unstructured":"Raad H, Derkawi RA, Tlili A, Belambri SA, Dang PM-C, El-Benna J. Phosphorylation of gp91 phox\/NOX2 in Human Neutrophils. NADPH Oxidases: Methods and Protocols. 2019:341\u2013\u200952.","DOI":"10.1007\/978-1-4939-9424-3_21"},{"key":"2062_CR25","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.2741\/3327","volume":"14","author":"EY Choi","year":"2009","unstructured":"Choi EY, Santoso S, Chavakis T. Mechanisms of neutrophil transendothelial migration. Front Bioscience: J Virtual Libr. 2009;14:1596.","journal-title":"Front Bioscience: J Virtual Libr"},{"key":"2062_CR26","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1590\/S0100-879X2010007500078","volume":"43","author":"NE Gomes","year":"2010","unstructured":"Gomes NE, Brunialti MKC, Mendes ME, Freudenberg M, Galanos C, Salom\u00e3o R. Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood. Braz J Med Biol Res. 2010;43:853\u20138.","journal-title":"Braz J Med Biol Res"},{"issue":"3","key":"2062_CR27","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1002\/jlb.67.3.396","volume":"67","author":"A Karlsson","year":"2000","unstructured":"Karlsson A, Nixon JB, McPhail LC. Phorbol myristate acetate induces neutrophil NADPH-oxidase activity by two separate signal transduction pathways: dependent or independent of phosphatidylinositol 3\u2010kinase. J Leukoc Biol. 2000;67(3):396\u2013404.","journal-title":"J Leukoc Biol"},{"issue":"8","key":"2062_CR28","doi-asserted-by":"publisher","first-page":"1234","DOI":"10.1172\/JCI18696","volume":"112","author":"J Fan","year":"2003","unstructured":"Fan J, Frey RS, Malik AB. TLR4 signaling induces TLR2 expression in endothelial cells via neutrophil NADPH oxidase. J Clin Investig. 2003;112(8):1234\u201343.","journal-title":"J Clin Investig"},{"key":"2062_CR29","doi-asserted-by":"crossref","unstructured":"Deptu\u0142a P, Fiedoruk K, Wasilewska M, Suprewicz \u0141, Cie\u015bluk M, \u017beliszewska P, et al. Physicochemical Nature of SARS-CoV-2 spike protein binding to human Vimentin. ACS Applied Materials & Interfaces; 2023.","DOI":"10.1021\/acsami.3c03347"},{"issue":"1","key":"2062_CR30","doi-asserted-by":"publisher","first-page":"4541","DOI":"10.1038\/s41467-020-18319-6","volume":"11","author":"J Yang","year":"2020","unstructured":"Yang J, Petitjean SJL, Koehler M, Zhang Q, Dumitru AC, Chen W, et al. Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. Nat Commun. 2020;11(1):4541.","journal-title":"Nat Commun"},{"issue":"1\u20134","key":"2062_CR31","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1081\/RRS-120014594","volume":"22","author":"M Horton","year":"2002","unstructured":"Horton M, Charras G, Lehenkari P. Analysis of ligand\u2013receptor interactions in cells by atomic force microscopy. J Recept Signal Transduction. 2002;22(1\u20134):169\u201390.","journal-title":"J Recept Signal Transduction"},{"issue":"1","key":"2062_CR32","doi-asserted-by":"publisher","first-page":"4460","DOI":"10.1038\/s41467-019-12411-2","volume":"10","author":"M Koehler","year":"2019","unstructured":"Koehler M, Aravamudhan P, Guzman-Cardozo C, Dumitru AC, Yang J, Gargiulo S, et al. Glycan-mediated enhancement of reovirus receptor binding. Nat Commun. 2019;10(1):4460.","journal-title":"Nat Commun"},{"key":"2062_CR33","first-page":"78","volume":"157","author":"A Lostao","year":"2021","unstructured":"Lostao A, Medina M. Atomic force microscopy: single-molecule imaging and force spectroscopy in the study of flavoproteins ligand binding and reaction mechanisms. Flavins Flavoproteins: Methods Protocols. 2021;157:78.","journal-title":"Flavins Flavoproteins: Methods Protocols"},{"issue":"5","key":"2062_CR34","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.1002\/eji.200323512","volume":"33","author":"C Rubel","year":"2003","unstructured":"Rubel C, G\u00f3mez S, Fern\u00e1ndez GC, Isturiz MA, Caama\u00f1o J, Palermo MS. Fibrinogen-CD11b\/CD18 interaction activates the NF\u2010\u03baB pathway and delays apoptosis in human neutrophils. Eur J Immunol. 2003;33(5):1429\u201338.","journal-title":"Eur J Immunol"},{"issue":"2","key":"2062_CR35","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1002\/ijc.29382","volume":"137","author":"A Mitra","year":"2015","unstructured":"Mitra A, Satelli A, Xia X, Cutrera J, Mishra L, Li S. Cell-surface V imentin: a mislocalized protein for isolating csV imentin\u2009+\u2009CD 133\u2013\u2009novel stem\u2010like hepatocellular carcinoma cells expressing EMT markers. Int J Cancer. 2015;137(2):491\u20136.","journal-title":"Int J Cancer"},{"issue":"12","key":"2062_CR36","doi-asserted-by":"publisher","first-page":"e29305","DOI":"10.1371\/journal.pone.0029305","volume":"6","author":"T P\u00e4ll","year":"2011","unstructured":"P\u00e4ll T, Pink A, Kasak L, Turkina M, Anderson W, Valkna A, et al. Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface. PLoS ONE. 2011;6(12):e29305.","journal-title":"PLoS ONE"},{"key":"2062_CR37","doi-asserted-by":"crossref","unstructured":"Menko AS, Romisher A, Walker JL. The pro-fibrotic response of mesenchymal leader cells to lens wounding involves hyaluronic acid, its receptor RHAMM, and vimentin. Front Cell Dev Biology. 2022;10.","DOI":"10.3389\/fcell.2022.862423"},{"issue":"3","key":"2062_CR38","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1093\/cvr\/cvt117","volume":"99","author":"PS Thiagarajan","year":"2013","unstructured":"Thiagarajan PS, Yakubenko VP, Elsori DH, Yadav SP, Willard B, Tan CD, et al. Vimentin is an endogenous ligand for the pattern recognition receptor Dectin-1. Cardiovascular Res. 2013;99(3):494\u2013504.","journal-title":"Cardiovascular Res"},{"issue":"2","key":"2062_CR39","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1152\/jappl.2001.91.2.613","volume":"91","author":"S Takashi","year":"2001","unstructured":"Takashi S, Okubo Y, Horie S. Contribution of CD54 to human eosinophil and neutrophil superoxide production. J Appl Physiol. 2001;91(2):613\u201322.","journal-title":"J Appl Physiol"},{"issue":"5","key":"2062_CR40","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1097\/00024382-199711000-00007","volume":"8","author":"JH Wang","year":"1997","unstructured":"Wang JH, Sexton DM, Redmond HP, Watson R, Croke DT, Bouchier-Hayes D. Intercellular adhesion molecule-1 (ICAM-1) is expressed on human neutrophils and is essential for neutrophil adherence and aggregation. Shock (Augusta Ga). 1997;8(5):357\u201361.","journal-title":"Shock (Augusta Ga)"},{"issue":"7","key":"2062_CR41","first-page":"898","volume":"127","author":"A Woodfin","year":"2016","unstructured":"Woodfin A, Beyrau M, Voisin M-B, Ma B, Whiteford JR, Hordijk PL, et al. ICAM-1\u2013expressing neutrophils exhibit enhanced effector functions in murine models of endotoxemia. Blood J Am Soc Hematol. 2016;127(7):898\u2013907.","journal-title":"Blood J Am Soc Hematol"},{"key":"2062_CR42","doi-asserted-by":"crossref","unstructured":"Kim H-M, Wang M, Kim S, Park J, Hong S-T. Removal of Extracellular Vimentin in Blood Confers Resistance to Viral Infection. 2022.","DOI":"10.21203\/rs.3.rs-2345254\/v1"},{"issue":"1","key":"2062_CR43","doi-asserted-by":"publisher","first-page":"5747","DOI":"10.1038\/s41598-019-42287-7","volume":"9","author":"L Su","year":"2019","unstructured":"Su L, Pan P, Yan P, Long Y, Zhou X, Wang X, et al. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis. Sci Rep. 2019;9(1):5747.","journal-title":"Sci Rep"},{"key":"2062_CR44","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.thromres.2022.11.028","volume":"221","author":"M Martinez-Vargas","year":"2023","unstructured":"Martinez-Vargas M, Cebula A, Brubaker LS, Seshadri N, Lam FW, Loor M, et al. A novel interaction between extracellular vimentin and fibrinogen in fibrin formation. Thromb Res. 2023;221:97\u2013104.","journal-title":"Thromb Res"},{"key":"2062_CR45","doi-asserted-by":"crossref","unstructured":"Ellis S, Way R, Nel M, Burleigh A, Doykov I, Kembou-Ringert J et al. Salivary IgA and vimentin differentiate in vitro SARS-CoV-2 infection: a study of 290 convalescent COVID-19 patients. Mucosal Immunol. 2023.","DOI":"10.1016\/j.mucimm.2023.11.007"},{"issue":"7","key":"2062_CR46","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1007\/s00109-020-01923-w","volume":"98","author":"S Kim","year":"2020","unstructured":"Kim S, Cho W, Kim I, Lee S-H, Oh GT, Park YM. Oxidized LDL induces vimentin secretion by macrophages and contributes to atherosclerotic inflammation. J Mol Med. 2020;98(7):973\u201383.","journal-title":"J Mol Med"},{"key":"2062_CR47","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.molimm.2018.09.017","volume":"104","author":"MB Yu","year":"2018","unstructured":"Yu MB, Guerra J, Firek A, Langridge WH. Extracellular vimentin modulates human dendritic cell activation. Mol Immunol. 2018;104:37\u201346.","journal-title":"Mol Immunol"},{"issue":"2","key":"2062_CR48","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1038\/ncb1355","volume":"8","author":"M Nieminen","year":"2006","unstructured":"Nieminen M, Henttinen T, Merinen M, Marttila\u2013Ichihara F, Eriksson JE, Jalkanen S. Vimentin function in lymphocyte adhesion and transcellular migration. Nat Cell Biol. 2006;8(2):156\u201362.","journal-title":"Nat Cell Biol"},{"key":"2062_CR49","doi-asserted-by":"crossref","unstructured":"Thalla DG, Rajwar AC, Laurent AM, Becher JE, Kainka L, Lautenschl\u00e4ger F. Extracellular vimentin is expressed at the rear of activated macrophage-like cells: potential role in enhancement of migration and phagocytosis. Front Cell Dev Biology. 2022;10.","DOI":"10.3389\/fcell.2022.891281"},{"issue":"15","key":"2062_CR50","doi-asserted-by":"publisher","first-page":"3958","DOI":"10.3390\/cancers15153958","volume":"15","author":"DJ Engbersen","year":"2023","unstructured":"Engbersen DJ, van Beijnum JR, Roos A, van Beelen M, de Haan JD, Grinwis GC, et al. Vaccination against Extracellular Vimentin for treatment of Urothelial Cancer of the bladder in client-owned dogs. Cancers. 2023;15(15):3958.","journal-title":"Cancers"},{"key":"2062_CR51","doi-asserted-by":"publisher","first-page":"792717","DOI":"10.3389\/fcvm.2022.792717","volume":"9","author":"SY Kim","year":"2022","unstructured":"Kim SY, Jeong S-J, Park J-H, Cho W, Ahn Y-H, Choi Y-H, et al. Plasma membrane localization of CD36 requires vimentin phosphorylation; a mechanism by which macrophage vimentin promotes atherosclerosis. Front Cardiovasc Med. 2022;9:792717.","journal-title":"Front Cardiovasc Med"},{"key":"2062_CR52","doi-asserted-by":"crossref","unstructured":"Arrindell J, Desnues B. Vimentin: from a cytoskeletal protein to a critical modulator of immune response and a target for infection. Front Immunol. 2023;14.","DOI":"10.3389\/fimmu.2023.1224352"},{"issue":"12","key":"2062_CR53","doi-asserted-by":"publisher","first-page":"2720","DOI":"10.3390\/cells9122720","volume":"9","author":"JS Kim","year":"2020","unstructured":"Kim JS, Choi M, Choi JY, Kim JY, Kim JY, Song J-S, et al. Implication of the association of fibrinogen citrullination and osteoclastogenesis in bone destruction in rheumatoid arthritis. Cells. 2020;9(12):2720.","journal-title":"Cells"},{"issue":"5","key":"2062_CR54","doi-asserted-by":"publisher","first-page":"e0251726","DOI":"10.1371\/journal.pone.0251726","volume":"16","author":"L Shi","year":"2021","unstructured":"Shi L, Aymonnier K, Wagner DD. Neutrophil stimulation with citrullinated histone H4 slows down calcium influx and reduces NET formation compared with native histone H4. PLoS ONE. 2021;16(5):e0251726.","journal-title":"PLoS ONE"},{"issue":"17","key":"2062_CR55","doi-asserted-by":"publisher","first-page":"9833","DOI":"10.3390\/ijms23179833","volume":"23","author":"LK Myers","year":"2022","unstructured":"Myers LK, Ouyang Y-X, Patel JR, Odens HH, Woo-Rasberry V, Park J, et al. Role of Citrullinated Collagen in Autoimmune Arthritis. Int J Mol Sci. 2022;23(17):9833.","journal-title":"Int J Mol Sci"},{"issue":"10","key":"2062_CR56","doi-asserted-by":"publisher","first-page":"2873","DOI":"10.1002\/art.30445","volume":"63","author":"O Snir","year":"2011","unstructured":"Snir O, Rieck M, Gebe JA, Yue BB, Rawlings CA, Nepom G, et al. Identification and functional characterization of T cells reactive to citrullinated vimentin in HLA\u2013DRB1* 0401\u2013positive humanized mice and rheumatoid arthritis patients. Arthr Rhuem. 2011;63(10):2873\u201383.","journal-title":"Arthr Rhuem"},{"issue":"5","key":"2062_CR57","doi-asserted-by":"publisher","first-page":"354","DOI":"10.3109\/03009742.2012.670263","volume":"41","author":"L Fan","year":"2012","unstructured":"Fan L, He D, Wang Q, Zong M, Zhang H, Yang L, et al. Citrullinated vimentin stimulates proliferation, pro-inflammatory cytokine secretion, and PADI4 and RANKL expression of fibroblast-like synoviocytes in rheumatoid arthritis. Scand J Rheumatol. 2012;41(5):354\u20138.","journal-title":"Scand J Rheumatol"},{"issue":"8","key":"2062_CR58","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.3390\/cells12081109","volume":"12","author":"S Shindo","year":"2023","unstructured":"Shindo S, Pierrelus R, Ikeda A, Nakamura S, Heidari A, Pastore MR, et al. Extracellular release of Citrullinated Vimentin directly acts on osteoclasts to promote bone resorption in a mouse model of Periodontitis. Cells. 2023;12(8):1109.","journal-title":"Cells"},{"issue":"12","key":"2062_CR59","doi-asserted-by":"publisher","first-page":"7199","DOI":"10.4049\/jimmunol.165.12.7199","volume":"165","author":"C Georganas","year":"2000","unstructured":"Georganas C, Liu H, Perlman H, Hoffmann A, Thimmapaya B, Pope RM. Regulation of IL-6 and IL-8 expression in rheumatoid arthritis synovial fibroblasts: the dominant role for NF-\u03baB but not C\/EBP\u03b2 or c-Jun. J Immunol. 2000;165(12):7199\u2013206.","journal-title":"J Immunol"},{"issue":"5","key":"2062_CR60","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1189\/jlb.2MR0316-117RR","volume":"100","author":"CA Leifer","year":"2016","unstructured":"Leifer CA, Medvedev AE. Molecular mechanisms of regulation of toll-like receptor signaling. J Leucocyte Biology. 2016;100(5):927\u201341.","journal-title":"J Leucocyte Biology"},{"issue":"10","key":"2062_CR61","doi-asserted-by":"publisher","first-page":"5451","DOI":"10.3390\/ijms25105451","volume":"25","author":"R Kircheis","year":"2024","unstructured":"Kircheis R. In Silico Analyses Indicate a lower potency for dimerization of TLR4\/MD-2 as the reason for the Lower pathogenicity of Omicron compared to wild-type virus and earlier SARS-CoV-2 variants. Int J Mol Sci. 2024;25(10):5451.","journal-title":"Int J Mol Sci"},{"issue":"4","key":"2062_CR62","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1038\/sj.emboj.7600991","volume":"25","author":"H Husebye","year":"2006","unstructured":"Husebye H, Halaas \u00d8, Stenmark H, Tunheim G, Sandanger \u00d8, Bogen B, et al. Endocytic pathways regulate toll-like receptor 4 signaling and link innate and adaptive immunity. EMBO J. 2006;25(4):683\u201392.","journal-title":"EMBO J"},{"key":"2062_CR63","doi-asserted-by":"crossref","unstructured":"Gangloff M. Different dimerisation mode for TLR4 upon endosomal acidification? Trends in biochemical sciences. 2012;37(3):92\u20138.","DOI":"10.1016\/j.tibs.2011.11.003"},{"issue":"5","key":"2062_CR64","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1016\/j.immuni.2015.10.008","volume":"43","author":"Y Tan","year":"2015","unstructured":"Tan Y, Zanoni I, Cullen TW, Goodman AL, Kagan JC. Mechanisms of toll-like receptor 4 endocytosis reveal a common immune-evasion strategy used by pathogenic and commensal bacteria. Immunity. 2015;43(5):909\u201322.","journal-title":"Immunity"},{"issue":"8","key":"2062_CR65","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1097\/CCM.0b013e3181e7c5c9","volume":"38","author":"TW Rice","year":"2010","unstructured":"Rice TW, Wheeler AP, Bernard GR, Vincent J-L, Angus DC, Aikawa N, et al. A randomized, double-blind, placebo-controlled trial of TAK-242 for the treatment of severe sepsis. Crit Care Med. 2010;38(8):1685\u201394.","journal-title":"Crit Care Med"},{"issue":"11","key":"2062_CR66","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1001\/jama.2013.2194","volume":"309","author":"SM Opal","year":"2013","unstructured":"Opal SM, Laterre P-F, Francois B, LaRosa SP, Angus DC, Mira J-P, et al. Effect of eritoran, an antagonist of MD2-TLR4, on mortality in patients with severe sepsis: the ACCESS randomized trial. JAMA. 2013;309(11):1154\u201362.","journal-title":"JAMA"},{"key":"2062_CR67","doi-asserted-by":"crossref","unstructured":"Bucki R, Iwamoto DV, Shi X, Kerr KE, Byfield FJ, Suprewicz \u0141 et al. Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures. J Biol Chem. 2023;299(8).","DOI":"10.1016\/j.jbc.2023.104963"},{"key":"2062_CR68","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.biomaterials.2016.11.012","volume":"115","author":"PP Partyka","year":"2017","unstructured":"Partyka PP, Godsey GA, Galie JR, Kosciuk MC, Acharya NK, Nagele RG, et al. Mechanical stress regulates transport in a compliant 3D model of the blood-brain barrier. Biomaterials. 2017;115:30\u20139.","journal-title":"Biomaterials"},{"issue":"1","key":"2062_CR69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12974-022-02642-4","volume":"19","author":"\u0141 Suprewicz","year":"2022","unstructured":"Suprewicz \u0141, Tran KA, Piktel E, Fiedoruk K, Janmey PA, Galie PA, et al. Recombinant human plasma gelsolin reverses increased permeability of the blood\u2013brain barrier induced by the spike protein of the SARS-CoV-2 virus. J Neuroinflamm. 2022;19(1):1\u201316.","journal-title":"J Neuroinflamm"},{"issue":"2","key":"2062_CR70","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.bbrc.2011.03.006","volume":"407","author":"Y Chen","year":"2011","unstructured":"Chen Y, Zeng G, Chen SS, Feng Q, Chen ZW. AFM force measurements of the gp120\u2013sCD4 and gp120 or CD4 antigen\u2013antibody interactions. Biochem Biophys Res Commun. 2011;407(2):301\u20136.","journal-title":"Biochem Biophys Res Commun"},{"issue":"10","key":"2062_CR71","doi-asserted-by":"publisher","first-page":"2878","DOI":"10.1128\/IAI.00538-16","volume":"84","author":"JF Miranda-Ozuna","year":"2016","unstructured":"Miranda-Ozuna JF, Hern\u00e1ndez-Garc\u00eda MS, Brieba LG, Ben\u00edtez-Cardoza CG, Ortega-L\u00f3pez J, Gonz\u00e1lez-Robles A, et al. The glycolytic enzyme triosephosphate isomerase of Trichomonas Vaginalis is a surface-associated protein induced by glucose that functions as a laminin-and fibronectin-binding protein. Infect Immun. 2016;84(10):2878\u201394.","journal-title":"Infect Immun"},{"key":"2062_CR72","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1016\/j.bjm.2017.10.002","volume":"49","author":"CR Bastos","year":"2018","unstructured":"Bastos CR, Mathias LA, Jusi MMG, Santos RFd, Silva GCPd, Andr\u00e9 MR, et al. Evaluation of dot-blot test for serological diagnosis of bovine brucellosis. Brazilian J Microbiol. 2018;49:564\u20138.","journal-title":"Brazilian J Microbiol"}],"container-title":["Cell Communication and Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-025-02062-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12964-025-02062-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-025-02062-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T17:44:44Z","timestamp":1738777484000},"score":1,"resource":{"primary":{"URL":"https:\/\/biosignaling.biomedcentral.com\/articles\/10.1186\/s12964-025-02062-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,5]]},"references-count":72,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2062"],"URL":"https:\/\/doi.org\/10.1186\/s12964-025-02062-w","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.05.02.592157","asserted-by":"object"}]},"ISSN":["1478-811X"],"issn-type":[{"value":"1478-811X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,5]]},"assertion":[{"value":"2 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Procedures with human blood samples from healthy donors were performed in accordance with the approval of the Medical University of Bialystok ethics committee No. APK.002.8.2023. All donors signed an informed consent form, in accordance with the relevant ethics committee approval.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"64"}}