{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T02:41:11Z","timestamp":1788230471978,"version":"build-2803163510"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":35,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Guangdong Nature Science Foundation","doi-asserted-by":"crossref","award":["2023A1515010374"],"award-info":[{"award-number":["2023A1515010374"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20220530145615034"],"award-info":[{"award-number":["JCYJ20220530145615034"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82302105"],"award-info":[{"award-number":["82302105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"DOI":"10.1186\/s12964-025-02556-7","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:04:21Z","timestamp":1764601461000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Reduced SUMOylation impairs NOTCH3 signaling and cell survival in the pathogenesis of CADASIL"],"prefix":"10.1186","volume":"24","author":[{"given":"Lijun","family":"Long","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danni","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuecheng","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suning","family":"Ping","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"issue":"7","key":"2556_CR1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S1474-4422(09)70127-9","volume":"8","author":"H Chabriat","year":"2009","unstructured":"Chabriat H, Joutel A, Dichgans M, Tournier-Lasserve E, Bousser MG. Cadasil. Lancet Neurol. 2009;8(7):643\u201353.","journal-title":"Lancet Neurol"},{"issue":"11","key":"2556_CR2","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1002\/acn3.344","volume":"3","author":"JW Rutten","year":"2016","unstructured":"Rutten JW, Dauwerse HG, Gravesteijn G, van Belzen MJ, van der Grond J, Polke JM, et al. Archetypal NOTCH3 mutations frequent in public exome: implications for CADASIL. Ann Clin Transl Neurol. 2016;3(11):844\u201353.","journal-title":"Ann Clin Transl Neurol"},{"key":"2556_CR3","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jare.2024.01.001","volume":"66","author":"L Yuan","year":"2024","unstructured":"Yuan L, Chen X, Jankovic J, Deng H. CADASIL: a NOTCH3-associated cerebral small vessel disease. J Adv Res. 2024;66:223\u201335.","journal-title":"J Adv Res"},{"issue":"6602","key":"2556_CR4","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/383707a0","volume":"383","author":"A Joutel","year":"1996","unstructured":"Joutel A, Corpechot C, Ducros A, Vahedi K, Chabriat H, Mouton P, et al. Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia. Nature. 1996;383(6602):707\u201310.","journal-title":"Nature"},{"issue":"5","key":"2556_CR5","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1172\/JCI8047","volume":"105","author":"A Joutel","year":"2000","unstructured":"Joutel A, Andreux F, Gaulis S, Domenga V, Cecillon M, Battail N, et al. The ectodomain of the Notch3 receptor accumulates within the cerebrovasculature of CADASIL patients. J Clin Invest. 2000;105(5):597\u2013605.","journal-title":"J Clin Invest"},{"issue":"2","key":"2556_CR6","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1172\/JCI39733","volume":"120","author":"A Joutel","year":"2010","unstructured":"Joutel A, Monet-Lepr\u00eatre M, Gosele C, Baron-Menguy C, Hammes A, Schmidt S, et al. Cerebrovascular dysfunction and microcirculation rarefaction precede white matter lesions in a mouse genetic model of cerebral ischemic small vessel disease. J Clin Invest. 2010;120(2):433\u201345.","journal-title":"J Clin Invest"},{"issue":"9298","key":"2556_CR7","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1016\/S0140-6736(01)07142-2","volume":"358","author":"A Joutel","year":"2001","unstructured":"Joutel A, Favrole P, Labauge P, Chabriat H, Lescoat C, Andreux F, et al. Skin biopsy immunostaining with a Notch3 monoclonal antibody for CADASIL diagnosis. Lancet. 2001;358(9298):2049\u201351.","journal-title":"Lancet"},{"key":"2556_CR8","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.nbd.2014.09.006","volume":"73","author":"XY Liu","year":"2015","unstructured":"Liu XY, Gonzalez-Toledo ME, Fagan A, Duan WM, Liu Y, Zhang S, et al. Stem cell factor and granulocyte colony-stimulating factor exhibit therapeutic effects in a mouse model of CADASIL. Neurobiol Dis. 2015;73:189\u2013203.","journal-title":"Neurobiol Dis"},{"issue":"8","key":"2556_CR9","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1161\/HYPERTENSIONAHA.122.20449","volume":"80","author":"HE Morris","year":"2023","unstructured":"Morris HE, Neves KB, Nilsen M, Montezano AC, MacLean MR, Touyz RM. Notch3\/Hes5 induces vascular dysfunction in hypoxia-induced pulmonary hypertension through ER stress and redox-sensitive pathways. Hypertension. 2023;80(8):1683\u201396.","journal-title":"Hypertension"},{"issue":"24","key":"2556_CR10","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.1042\/CS20190835","volume":"133","author":"HE Morris","year":"2019","unstructured":"Morris HE, Neves KB, Montezano AC, MacLean MR, Touyz RM. Notch3 signalling and vascular remodelling in pulmonary arterial hypertension. Clin Sci (Lond). 2019;133(24):2481\u201398.","journal-title":"Clin Sci (Lond)"},{"key":"2556_CR11","doi-asserted-by":"crossref","unstructured":"Malka K, Liaw L. NOTCH3 as a modulator of vascular disease: a target in Elastin deficiency and arterial pathologies. J Clin Invest. 2022;132(5):e157007.","DOI":"10.1172\/JCI157007"},{"key":"2556_CR12","doi-asserted-by":"crossref","unstructured":"Hosseini-Alghaderi S, Baron M. Notch3 in Development, health and disease. Biomolecules. 2020;10(3):485.","DOI":"10.3390\/biom10030485"},{"issue":"6","key":"2556_CR13","doi-asserted-by":"crossref","first-page":"H1292","DOI":"10.1152\/ajpheart.00530.2019","volume":"317","author":"YR Kim","year":"2019","unstructured":"Kim YR, Jacobs JS, Li Q, Gaddam RR, Vikram A, Liu J, et al. SUMO2 regulates vascular endothelial function and oxidative stress in mice. Am J Physiol Heart Circ Physiol. 2019;317(6):H1292\u2013300.","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"1","key":"2556_CR14","doi-asserted-by":"crossref","first-page":"6919","DOI":"10.1038\/s41467-024-51350-5","volume":"15","author":"Y Xu","year":"2024","unstructured":"Xu Y, Zhang H, Chen Y, Pober JS, Zhou M, Zhou JH, et al. SRF sumoylation modulates smooth muscle phenotypic switch and vascular remodeling. Nat Commun. 2024;15(1):6919.","journal-title":"Nat Commun"},{"issue":"11","key":"2556_CR15","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/s41580-022-00500-y","volume":"23","author":"ACO Vertegaal","year":"2022","unstructured":"Vertegaal ACO. Signalling mechanisms and cellular functions of SUMO. Nat Rev Mol Cell Biol. 2022;23(11):715\u201331.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2556_CR16","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev.biochem.73.011303.074118","volume":"73","author":"ES Johnson","year":"2004","unstructured":"Johnson ES. Protein modification by SUMO. Annu Rev Biochem. 2004;73:355\u201382.","journal-title":"Annu Rev Biochem"},{"issue":"Web Server issu","key":"2556_CR17","doi-asserted-by":"crossref","first-page":"W325","DOI":"10.1093\/nar\/gku383","volume":"42","author":"Q Zhao","year":"2014","unstructured":"Zhao Q, Xie Y, Zheng Y, Jiang S, Liu W, Mu W, et al. GPS-SUMO: a tool for the prediction of sumoylation sites and SUMO-interaction motifs. Nucleic Acids Res. 2014;42(Web Server issue):W325\u201330.","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"2556_CR18","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1161\/CIRCRESAHA.117.310696","volume":"121","author":"X Zhu","year":"2017","unstructured":"Zhu X, Ding S, Qiu C, Shi Y, Song L, Wang Y, et al. SUMOylation negatively regulates angiogenesis by targeting endothelial NOTCH signaling. Circ Res. 2017;121(6):636\u201349.","journal-title":"Circ Res"},{"issue":"2","key":"2556_CR19","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1161\/CIRCRESAHA.123.323398","volume":"134","author":"R Ren","year":"2024","unstructured":"Ren R, Ding S, Ma K, Jiang Y, Wang Y, Chen J, et al. SUMOylation fine-tunes endothelial HEY1 in the regulation of angiogenesis. Circ Res. 2024;134(2):203\u201322.","journal-title":"Circ Res"},{"issue":"3","key":"2556_CR20","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/s41418-017-0002-6","volume":"25","author":"CJM Antila","year":"2018","unstructured":"Antila CJM, Rraklli V, Blomster HA, Dahlstr\u00f6m KM, Salminen TA, Holmberg J, et al. Sumoylation of Notch1 represses its target gene expression during cell stress. Cell Death Differ. 2018;25(3):600\u201315.","journal-title":"Cell Death Differ"},{"issue":"4","key":"2556_CR21","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1177\/0963689718766460","volume":"27","author":"S Ping","year":"2018","unstructured":"Ping S, Qiu X, Gonzalez-Toledo ME, Liu X, Zhao LR. Stem cell factor in combination with granulocyte Colony-Stimulating factor reduces cerebral capillary thrombosis in a mouse model of CADASIL. Cell Transpl. 2018;27(4):637\u201347.","journal-title":"Cell Transpl"},{"key":"2556_CR22","doi-asserted-by":"crossref","DOI":"10.1016\/j.nbd.2019.104561","volume":"132","author":"S Ping","year":"2019","unstructured":"Ping S, Qiu X, Kyle M, Hughes K, Longo J, Zhao LR. Stem cell factor and granulocyte colony-stimulating factor promote brain repair and improve cognitive function through VEGF-A in a mouse model of CADASIL. Neurobiol Dis. 2019;132:104561.","journal-title":"Neurobiol Dis"},{"key":"2556_CR23","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2020.00803","volume":"8","author":"S Ping","year":"2020","unstructured":"Ping S, Qiu X, Gonzalez-Toledo ME, Liu X, Zhao LR. Stem cell factor in combination with granulocyte Colony-Stimulating factor protects the brain from capillary thrombosis-induced ischemic neuron loss in a mouse model of CADASIL. Front Cell Dev Biol. 2020;8:627733.","journal-title":"Front Cell Dev Biol"},{"key":"2556_CR24","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/978-1-61779-551-0_18","volume":"849","author":"SA Gauthier","year":"2012","unstructured":"Gauthier SA, Sahoo S, Jung SS, Levy E. Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain. Methods Mol Biol. 2012;849:261\u201374.","journal-title":"Methods Mol Biol"},{"key":"2556_CR25","volume-title":"Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM","author":"RP Haugland","year":"1994","unstructured":"Haugland RP, MacCoubrey IC, Moore PL. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM. Google Patents; 1994."},{"issue":"3","key":"2556_CR26","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9","volume":"182","author":"T Scholzen","year":"2000","unstructured":"Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol. 2000;182(3):311\u201322.","journal-title":"J Cell Physiol"},{"issue":"25","key":"2556_CR27","doi-asserted-by":"crossref","first-page":"40514","DOI":"10.18632\/oncotarget.17121","volume":"8","author":"PD Pereira","year":"2017","unstructured":"Pereira PD, Serra-Caetano A, Cabrita M, Bekman E, Braga J, Rino J, et al. Quantification of cell cycle kinetics by edu (5-ethynyl-2\u2019-deoxyuridine)-coupled-fluorescence-intensity analysis. Oncotarget. 2017;8(25):40514\u201332.","journal-title":"Oncotarget"},{"key":"2556_CR28","volume":"3","author":"H Chabriat","year":"2022","unstructured":"Chabriat H, Lesnik Oberstein S. Cognition, mood and behavior in CADASIL. Cereb Circ. 2022;3:100043.","journal-title":"Cereb Circ"},{"issue":"5","key":"2556_CR29","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/nan.12599","volume":"46","author":"P Bourassa","year":"2020","unstructured":"Bourassa P, Tremblay C, Schneider JA, Bennett DA, Calon F. Brain mural cell loss in the parietal cortex in Alzheimer\u2019s disease correlates with cognitive decline and TDP-43 pathology. Neuropathol Appl Neurobiol. 2020;46(5):458\u201377.","journal-title":"Neuropathol Appl Neurobiol"},{"key":"2556_CR30","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jchemneu.2018.06.001","volume":"92","author":"LCD Smyth","year":"2018","unstructured":"Smyth LCD, Rustenhoven J, Scotter EL, Schweder P, Faull RLM, Park TIH, et al. Markers for human brain pericytes and smooth muscle cells. J Chem Neuroanat. 2018;92:48\u201360.","journal-title":"J Chem Neuroanat"},{"issue":"11","key":"2556_CR31","doi-asserted-by":"crossref","first-page":"9171","DOI":"10.1007\/s12035-024-04141-4","volume":"61","author":"Y Ahn","year":"2024","unstructured":"Ahn Y, An JH, Yang HJ, Lee WJ, Lee SH, Park YH, et al. Blood vessel organoids generated by base editing and harboring single nucleotide variation in Notch3 effectively recapitulate CADASIL-related pathogenesis. Mol Neurobiol. 2024;61(11):9171\u201383.","journal-title":"Mol Neurobiol"},{"issue":"26","key":"2556_CR32","doi-asserted-by":"crossref","first-page":"16226","DOI":"10.1074\/jbc.M115.655548","volume":"290","author":"JT Baeten","year":"2015","unstructured":"Baeten JT, Lilly B. Differential regulation of NOTCH2 and NOTCH3 contribute to their unique functions in vascular smooth muscle cells. J Biol Chem. 2015;290(26):16226\u201337.","journal-title":"J Biol Chem"},{"issue":"10","key":"2556_CR33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0077299","volume":"8","author":"MA Alqudah","year":"2013","unstructured":"Alqudah MA, Agarwal S, Al-Keilani MS, Sibenaller ZA, Ryken TC, Assem M. NOTCH3 is a prognostic factor that promotes glioma cell proliferation, migration and invasion via activation of CCND1 and EGFR. PLoS One. 2013;8(10):e77299.","journal-title":"PLoS One"},{"issue":"7","key":"2556_CR34","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1097\/nen.0b013e318093e574","volume":"66","author":"F Gray","year":"2007","unstructured":"Gray F, Polivka M, Viswanathan A, Baudrimont M, Bousser MG, Chabriat H. Apoptosis in cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy. J Neuropathol Exp Neurol. 2007;66(7):597\u2013607.","journal-title":"J Neuropathol Exp Neurol"},{"issue":"6","key":"2556_CR35","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1111\/jcmm.13534","volume":"22","author":"M Panahi","year":"2018","unstructured":"Panahi M, Yousefi Mesri N, Samuelsson EB, Coupland KG, Forsell C, Graff C, et al. Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGF\u03b2 expression. J Cell Mol Med. 2018;22(6):3016\u201324.","journal-title":"J Cell Mol Med"},{"issue":"1","key":"2556_CR36","volume":"7","author":"B Zhou","year":"2022","unstructured":"Zhou B, Lin W, Long Y, Yang Y, Zhang H, Wu K, et al. Notch signaling pathway: architecture, disease, and therapeutics. Signal Transduct Target Ther. 2022;7(1):95.","journal-title":"Signal Transduct Target Ther"},{"key":"2556_CR37","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2024.116655","volume":"282","author":"L Zhou","year":"2024","unstructured":"Zhou L, Huang C, HuangFu C, Shen P, Hu Y, Wang N, et al. Low-dose radiation-induced SUMOylation of NICD1 negatively regulates osteogenic differentiation in BMSCs. Ecotoxicol Environ Saf. 2024;282:116655.","journal-title":"Ecotoxicol Environ Saf"},{"issue":"8","key":"2556_CR38","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1093\/hmg\/ddm042","volume":"16","author":"M Monet","year":"2007","unstructured":"Monet M, Domenga V, Lemaire B, Souilhol C, Langa F, Babinet C, et al. The archetypal R90C CADASIL-NOTCH3 mutation retains NOTCH3 function in vivo. Hum Mol Genet. 2007;16(8):982\u201392.","journal-title":"Hum Mol Genet"},{"key":"2556_CR39","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/bs.apha.2016.07.002","volume":"78","author":"JT Baeten","year":"2017","unstructured":"Baeten JT, Lilly B. Notch signaling in vascular smooth muscle cells. Adv Pharmacol. 2017;78:351\u201382.","journal-title":"Adv Pharmacol"},{"key":"2556_CR40","doi-asserted-by":"crossref","unstructured":"Romay MC, Knutsen RH, Ma F, Mompe\u00f3n A, Hernandez GE, Salvador J et al. Age-related loss of Notch3 underlies brain vascular contractility deficiencies, glymphatic dysfunction, and neurodegeneration in mice. J Clin Invest. 2024;134(2):e166134.","DOI":"10.1172\/JCI166134"},{"issue":"8","key":"2556_CR41","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.ejcb.2018.10.001","volume":"97","author":"ES Hanemaaijer","year":"2018","unstructured":"Hanemaaijer ES, Panahi M, Swaddiwudhipong N, Tikka S, Winblad B, Viitanen M, et al. Autophagy-lysosomal defect in human CADASIL vascular smooth muscle cells. Eur J Cell Biol. 2018;97(8):557\u201367.","journal-title":"Eur J Cell Biol"},{"issue":"3","key":"2556_CR42","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.yexcr.2012.09.015","volume":"319","author":"M Viitanen","year":"2013","unstructured":"Viitanen M, Sundstr\u00f6m E, Baumann M, Poyhonen M, Tikka S, Behbahani H. Experimental studies of mitochondrial function in CADASIL vascular smooth muscle cells. Exp Cell Res. 2013;319(3):134\u201343.","journal-title":"Exp Cell Res"},{"issue":"1","key":"2556_CR43","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s40478-023-01558-1","volume":"11","author":"M Panahi","year":"2023","unstructured":"Panahi M, Hase Y, Gallart-Palau X, Mitra S, Watanabe A, Low RC, et al. ER stress induced immunopathology involving complement in CADASIL: implications for therapeutics. Acta Neuropathol Commun. 2023;11(1):76.","journal-title":"Acta Neuropathol Commun"},{"issue":"10","key":"2556_CR44","doi-asserted-by":"crossref","first-page":"4262","DOI":"10.1128\/MCB.25.10.4262-4271.2005","volume":"25","author":"K Murata","year":"2005","unstructured":"Murata K, Hattori M, Hirai N, Shinozuka Y, Hirata H, Kageyama R, et al. Hes1 directly controls cell proliferation through the transcriptional repression of p27Kip1. Mol Cell Biol. 2005;25(10):4262\u201371.","journal-title":"Mol Cell Biol"},{"issue":"12","key":"2556_CR45","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1161\/ATVBAHA.111.237859","volume":"31","author":"G Wallays","year":"2011","unstructured":"Wallays G, Nuyens D, Silasi-Mansat R, Souffreau J, Callaerts-Vegh Z, Van Nuffelen A, et al. Notch3 Arg170Cys knock-in mice display pathologic and clinical features of the neurovascular disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Arterioscler Thromb Vasc Biol. 2011;31(12):2881\u20138.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"11","key":"2556_CR46","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1161\/STROKEAHA.118.021560","volume":"49","author":"K Coupland","year":"2018","unstructured":"Coupland K, Lendahl U, Karlstr\u00f6m H. Role of NOTCH3 mutations in the cerebral small vessel disease cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Stroke. 2018;49(11):2793\u2013800.","journal-title":"Stroke"},{"issue":"2","key":"2556_CR47","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.cell.2009.03.045","volume":"137","author":"R Kopan","year":"2009","unstructured":"Kopan R, Ilagan MX. The canonical Notch signaling pathway: unfolding the activation mechanism. Cell. 2009;137(2):216\u201333.","journal-title":"Cell"},{"issue":"45","key":"2556_CR48","volume":"9","author":"R Guo","year":"2023","unstructured":"Guo R, Han D, Song X, Gao Y, Li Z, Li X, et al. Context-dependent regulation of Notch signaling in glial development and tumorigenesis. Sci Adv. 2023;9(45):eadi2167.","journal-title":"Sci Adv"},{"issue":"5","key":"2556_CR49","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.devcel.2009.03.010","volume":"16","author":"ME Fortini","year":"2009","unstructured":"Fortini ME. Notch signaling: the core pathway and its posttranslational regulation. Dev Cell. 2009;16(5):633\u201347.","journal-title":"Dev Cell"},{"issue":"1","key":"2556_CR50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/bi901263m","volume":"49","author":"SM Mooney","year":"2010","unstructured":"Mooney SM, Grande JP, Salisbury JL, Janknecht R. Sumoylation of p68 and p72 RNA helicases affects protein stability and transactivation potential. Biochemistry. 2010;49(1):1\u201310.","journal-title":"Biochemistry"},{"key":"2556_CR51","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/s42003-019-0428-0","volume":"2","author":"L Ranjha","year":"2019","unstructured":"Ranjha L, Levikova M, Altmannova V, Krejci L, Cejka P. Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2. Commun Biol. 2019;2:174.","journal-title":"Commun Biol"},{"issue":"6","key":"2556_CR52","doi-asserted-by":"crossref","first-page":"e0130275","DOI":"10.1371\/journal.pone.0130275","volume":"10","author":"H Ramachandran","year":"2015","unstructured":"Ramachandran H, Herfurth K, Grosschedl R, Sch\u00e4fer T, Walz G. SUMOylation blocks the Ubiquitin-Mediated degradation of the nephronophthisis gene product Glis2\/NPHP7. PLoS One. 2015;10(6):e0130275.","journal-title":"PLoS One"}],"container-title":["Cell Communication and Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-025-02556-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12964-025-02556-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12964-025-02556-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T16:52:50Z","timestamp":1767631970000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12964-025-02556-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["2556"],"URL":"https:\/\/doi.org\/10.1186\/s12964-025-02556-7","relation":{},"ISSN":["1478-811X"],"issn-type":[{"value":"1478-811X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,1]]},"assertion":[{"value":"15 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were conducted in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals. All procedures were approved by the Animal Ethics Committee at the School of Medicine, Sun Yat-sen University, with the ethical number SYSU-IACUC-MED-2022-B078.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3"}}