{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:42:49Z","timestamp":1787877769503,"version":"build-2784847793"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":55,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100005039","name":"Leids Universitair Medisch Centrum","doi-asserted-by":"publisher","award":["LUMC-PhD grant for the project UNIONS"],"award-info":[{"award-number":["LUMC-PhD grant for the project UNIONS"]}],"id":[{"id":"10.13039\/501100005039","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21CC0073729"],"award-info":[{"award-number":["NNF21CC0073729"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013297","name":"Eurostars","doi-asserted-by":"publisher","award":["113601"],"award-info":[{"award-number":["113601"]}],"id":[{"id":"10.13039\/100013297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["024.003.001"],"award-info":[{"award-number":["024.003.001"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["10250022110004"],"award-info":[{"award-number":["10250022110004"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101001746"],"award-info":[{"award-number":["101001746"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Commun Signal"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The bidirectional communication between the heart and kidney is essential for physiological homeostasis, with injury in one organ often impairing the other. Although cardiorenal crosstalk is clinically relevant in conditions such as cardiorenal syndrome (CRS), the underlying molecular and cellular mechanisms remain poorly understood, and in vitro models are lacking. Here, we developed a co-culture system using human induced pluripotent stem cell (hiPSC)-derived kidney organoids (kOs) and cardiac microtissues (cMTs) to model the cardiorenal axis.<\/jats:p>\n                  <jats:p>kOs exposed to nephrotoxic compounds for 72\u00a0h displayed glomerular and tubular damage, reduced cell viability, and altered gene expression. When subsequently co-cultured with cMTs for 72\u00a0h, injured kOs induced secondary cardiac dysfunction characterized by reduced cell viability, impaired contractility, and endothelial cell loss. These findings demonstrate that kidney injury can elicit detrimental effects on cardiac tissues in vitro. This organoid-based platform offers a valuable tool for studying cardiorenal interactions and underlines the potential of multi-organ models for investigating mechanisms of interdependent organ dysfunction.<\/jats:p>","DOI":"10.1186\/s12964-026-02902-3","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T10:46:17Z","timestamp":1778064377000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In vitro modeling of renal injury-induced cardiac effects using human iPSC-derived organoids"],"prefix":"10.1186","volume":"24","author":[{"given":"Beatrice","family":"Gabbin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Gallant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangchen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailiang","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berend J.","family":"van Meer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton J.","family":"Rabelink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine L.","family":"Mummery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Vanslambrouck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathelijne W.","family":"van den Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Meraviglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Bellin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,6]]},"reference":[{"key":"2902_CR1","doi-asserted-by":"crossref","unstructured":"Ronco C, Haapio M, House AA, Anavekar N, Bellomo R. 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Sept., Gentamicin-induced acute kidney injury in an animal model involves programmed necrosis of the collecting duct, J. Am. Soc. Nephrol. 2020;31(9):2097\u20132115.","DOI":"10.1681\/ASN.2019020204"},{"key":"2902_CR8","doi-asserted-by":"crossref","unstructured":"Hofbauer P, Jahnel SM, Mendjan S. In vitro models of the human heart, Development, 2021;148(16).","DOI":"10.1242\/dev.199672"},{"key":"2902_CR9","doi-asserted-by":"crossref","unstructured":"Tekguc M et al. June., Kidney Organoids: A pioneering model for kidney diseases, Transl. Res., 2022.","DOI":"10.1016\/j.trsl.2022.06.012"},{"key":"2902_CR10","doi-asserted-by":"crossref","unstructured":"Liu S, Fang C, Zhong C, Li J, Xiao Q. Recent advances in pluripotent stem cell-derived cardiac organoids and heart-on-chip applications for studying anti-cancer drug-induced cardiotoxicity, Cell Biol. 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Oct., Dysregulation of the 3\u03b2-hydroxysteroid dehydrogenase type 2 enzyme and steroid hormone biosynthesis in chronic kidney disease, Front. Endocrinol. (Lausanne), 2024;15:1358124.","DOI":"10.3389\/fendo.2024.1358124"},{"key":"2902_CR38","doi-asserted-by":"crossref","unstructured":"Wagner N, Wagner K-D, Theres H, Englert C, Schedl A, Scholz H. Coronary vessel development requires activation of the TrkB neurotrophin receptor by the Wilms\u2019 tumor transcription factor Wt1, Genes Dev., vol. 2005;19(21):2631\u20132642.","DOI":"10.1101\/gad.346405"},{"key":"2902_CR39","doi-asserted-by":"crossref","unstructured":"Liu Y, Guan X, Shao Y, Zhou J, Huang Y. The molecular mechanism and therapeutic strategy of cardiorenal syndrome type 3. Rev Cardiovasc Med. Feb. 2023;24(2):52.","DOI":"10.31083\/j.rcm2402052"},{"key":"2902_CR40","doi-asserted-by":"crossref","unstructured":"Akcay A, Nguyen Q, Edelstein CL. Mediators of inflammation in acute kidney injury. Mediators Inflamm. 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Mar., Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo, Stem Cell Reports, vol. 2018;10(3):751\u2013765.","DOI":"10.1016\/j.stemcr.2018.01.041"},{"key":"2902_CR51","doi-asserted-by":"crossref","unstructured":"Sala L et al. Feb., MUSCLEMOTION: A Versatile Open Software Tool to Quantify Cardiomyocyte and Cardiac Muscle Contraction In Vitro and In Vivo, Circ. Res. 2018;122(3):e5\u2013e16.","DOI":"10.1161\/CIRCRESAHA.117.312067"},{"key":"2902_CR52","doi-asserted-by":"crossref","unstructured":"Stein JM et al. Software tool for automatic quantification of sarcomere length and organization in fixed and live 2D and 3D muscle cell cultures in vitro. Curr Protoc, 2022;2(7):e462.","DOI":"10.1002\/cpz1.462"},{"key":"2902_CR53","doi-asserted-by":"crossref","unstructured":"Vanslambrouck JM et al. 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L. M. is co-founder of Pluriomics (now Ncardia b.v.) and B. J. v. M. is co-founder and CTO of Demcon Biovitronix. The other authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"380"}}