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Human conjunctival epithelial cells (HCEs) were cultured and treated with transforming growth factor \u03b2 (TGF-\u03b2) and epidermal growth factor (EGF) and transfected with miR-199a-3p\/5p mimic and inhibitor. Markers of epithelial-mesenchymal transition (EMT) in HCEs were detected using western blot and immunohistochemistry. Cell migration ability was determined using wound healing and transwell assay, while apoptosis was determined by flow cytometry. The target genes of miR-199a were confirmed by the dual-luciferase reporter assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TGF-\u03b2 and EGF could induced EMT in HCEs and increase miR-199a-3p\/5p but suppress target genes, DUSP5 and MAP3K11. With the occurrence of EMT, cell migration ability was enhanced, and apoptosis was impeded. Promoting miR-199a-3p\/5p expression could induce EMT in HCEs without TGF-\u03b2 and EGF, while suppressing miR-199a-3p\/5p could inhibit EMT in TGF-\u03b2 and EGF induced HCEs. In a word, TGF-\u03b2 and EGF induced EMT could be regulated with miR-199a-3p\/5p-DUSP5\/MAP3K11 axes. The validated results in tissues showed that, compared with control conjunctival tissues, miR-199a-3p\/5p were more overexpressed in pterygium, while DUSP5\/MAP3K11 were lower expressed. In addition, bioinformatics analysis indicated the miR-199a-3p\/5p-DUSP5\/MAP3K11 was belong to MAPK signalling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>TGF-\u03b2 and EGF induce EMT of HCEs through miR-199a-3p\/5p-DUSP5\/MAP3K11 axes, which explains the pathogenesis of EMT in pterygium and may provide new targets for pterygium prevention and therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12967-020-02499-2","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T20:02:23Z","timestamp":1598904143000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["MiR-199a-3p\/5p participated in TGF-\u03b2 and EGF induced EMT by targeting DUSP5\/MAP3K11 in pterygium"],"prefix":"10.1186","volume":"18","author":[{"given":"Siying","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9150-0614","authenticated-orcid":false,"given":"Ming","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2499_CR1","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1093\/ije\/dyn017","volume":"37","author":"RM Lucas","year":"2008","unstructured":"Lucas RM, McMichael AJ, Armstrong BK, Smith WT. 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