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Karola Marcinkowskiego w Poznaniu","doi-asserted-by":"publisher","award":["502-14-02229373-09513"],"award-info":[{"award-number":["502-14-02229373-09513"]}],"id":[{"id":"10.13039\/501100010438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Steroid resistant (SR) nephrotic syndrome (NS) affects up to 30% of children and is responsible for fast progression to end stage renal disease. Currently there is no early prognostic marker of SR and studied candidate variants and parameters differ highly between distinct ethnic cohorts.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Here, we analyzed 11polymorphic variants, 6 mutations, <jats:italic>SOCS3<\/jats:italic> promoter methylation and biochemical parameters as prognostic markers in a group of 124 Polish NS children (53 steroid resistant, 71 steroid sensitive including 31 steroid dependent) and 55 controls. We used single marker and multiple logistic regression analysis, accompanied by prediction modeling using neural network approach.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We achieved 92% (AUC\u2009=\u20090.778) SR prediction for binomial and 63% for multinomial calculations, with the strongest predictors <jats:italic>ABCB1<\/jats:italic> rs1922240, rs1045642 and rs2235048, <jats:italic>CD73<\/jats:italic> rs9444348 and rs4431401, serum creatinine and unmethylated <jats:italic>SOCS3<\/jats:italic> promoter region. Next, we achieved 80% (AUC\u2009=\u20090.720) in binomial and 63% in multinomial prediction of SD, with the strongest predictors <jats:italic>ABCB1<\/jats:italic> rs1045642 and rs2235048. Haplotype analysis revealed <jats:italic>CD73<\/jats:italic>_AG to be associated with SR while <jats:italic>ABCB1<\/jats:italic>_AGT was associated with SR, SD and membranoproliferative pattern of kidney injury regardless the steroid response.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We achieved prediction of steroid resistance and, as a novelty, steroid dependence, based on early markers in NS children. Such predictions, prior to drug administration, could facilitate decision on a proper treatment and avoid diverse effects of high steroid doses.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12967-021-02790-w","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T14:02:59Z","timestamp":1617112979000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Prediction of steroid resistance and steroid dependence in nephrotic syndrome children"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-781X","authenticated-orcid":false,"given":"Katarzyna","family":"Zaorska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Zawierucha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"\u015awierczewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danuta","family":"Ostalska-Nowicka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Zachwieja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Nowicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"2790_CR1","doi-asserted-by":"publisher","first-page":"119","DOI":"10.2147\/IJNRD.S193874","volume":"12","author":"EA Anigilaje","year":"2019","unstructured":"Anigilaje EA, Olutola A. 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