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Langbert Neuroimmunology Research Fund"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Interstitial cystitis\/bladder pain syndrome (IC\/BPS) is a disorder characterized by bladder pain upon filling which severely affects quality of life. Clinical presentation can vary. Local inflammatory events typify the clinical presentation of IC\/BPS patients with Hunner lesions (IC\/BPS-HL). It has previously been proposed that B cells are more prevalent in HL, but understanding their exact role in this environment requires a more complete immunological profile of HL. We characterized immunological dysfunction specifically in HL using immunohistochemistry. We detected significantly more plasma cells (50\u00d7 increase, p\u2009&lt;\u20090.0001), B cells (28\u00d7 increase, p\u2009&lt;\u20090.0001), T cells (3\u00d7 increase, p\u2009&lt;\u20090.0001), monocytes\/macrophages (6\u00d7 increase, p\u2009&lt;\u20090.0001), granulocytes (4\u00d7 increase, p\u2009&lt;\u20090.0001), and natural killer cells (2\u00d7 increase, p\u2009=\u20090.0249) in IC\/BPS patients with HL than in unaffected controls (UC). Patients with IC\/BPS-HL also had significantly elevated urinary levels of IL-6 (p\u2009=\u20090.0054), TNF-\u03b1 (p\u2009=\u20090.0064) and IL-13 (p\u2009=\u20090.0304) compared to patients with IC\/BPS without HL (IC\/BPS-NHL). In contrast, IL-12p70 levels were significantly lower in the patients with HL than in those without these lesions (p\u2009=\u20090.0422). Different cytokines were elevated in the urine of IC\/BPS patients with and without HL, indicating that different disease processes are active in IC\/BPS patients with and without HL. Elevated levels of CD138+, CD20+, and CD3+ cells in HL are consistent B and T-cell involvement in disease processes within HL.<\/jats:p>","DOI":"10.1186\/s12967-022-03236-7","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T04:04:13Z","timestamp":1645502653000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Immune cell profiles of patients with interstitial cystitis\/bladder pain syndrome"],"prefix":"10.1186","volume":"20","author":[{"given":"Robert M.","family":"Moldwin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishaan","family":"Nursey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oksana","family":"Yaskiv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddhartha","family":"Dalvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Macdonald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Funaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"DeGouveia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ruzimovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horacio R.","family":"Rilo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmund J.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souhel","family":"Najjar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna","family":"Tabansky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9607-9064","authenticated-orcid":false,"given":"Joel N. 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Informed consent was obtained from all research participants prior to sample acquisition and health information retrieval. The study approval number is 17-0254-NSUH.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}],"article-number":"97"}}