{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T05:31:43Z","timestamp":1740807103746,"version":"3.38.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the top medical expert team of the 2021 \u201cTaihu Talent Program\u201d in Wuxi","award":["WXTTP-202010"],"award-info":[{"award-number":["WXTTP-202010"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"DOI":"10.1186\/s12967-024-05840-1","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T12:49:24Z","timestamp":1740746964000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bone marrow fluid enhances the osteogenic activity of induced membrane leading to spontaneous osteogenesis: experimental validation and application in tibiofibular fusion for support reconstruction of segmental tibial defects"],"prefix":"10.1186","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7347-1773","authenticated-orcid":false,"given":"Qudong","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihao","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongwei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6950-1550","authenticated-orcid":false,"given":"Fanyu","family":"Bu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"issue":"1","key":"5840_CR1","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1111\/os.13604","volume":"15","author":"Y Lu","year":"2023","unstructured":"Lu Y, Lai CY, Lai PJ, Yu YH. Induced membrane technique for the management of segmental femoral defects: a systematic review and meta-analysis of individual participant data. Orthop Surg. 2023;15(1):28\u201337. https:\/\/doi.org\/10.1111\/os.13604.","journal-title":"Orthop Surg"},{"issue":"3","key":"5840_CR2","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.otsr.2019.01.007","volume":"105","author":"J Wang","year":"2019","unstructured":"Wang J, Yin Q, Gu S, Wu Y, Rui Y. Induced membrane technique in the treatment of infectious bone defect: a clinical analysis. Orthop Traumatol Surg Res. 2019;105(3):535\u20139. https:\/\/doi.org\/10.1016\/j.otsr.2019.01.007.","journal-title":"Orthop Traumatol Surg Res"},{"issue":"Suppl 5","key":"5840_CR3","doi-asserted-by":"publisher","first-page":"S36","DOI":"10.1097\/BOT.0000000000000979","volume":"31","author":"AC Masquelet","year":"2017","unstructured":"Masquelet AC. Induced membrane technique: pearls and pitfalls. J Orthop Trauma. 2017;31(Suppl 5):S36\u201338. https:\/\/doi.org\/.","journal-title":"J Orthop Trauma"},{"issue":"3","key":"5840_CR4","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1111\/os.12873","volume":"13","author":"L Zhang","year":"2021","unstructured":"Zhang L, Lu C, Lv Y, Wang X, Guo S, Zhang H. Three-dimensional printing-assisted Masquelet technique in the treatment of calcaneal defects. Orthop Surg. 2021;13(3):876\u201383. https:\/\/doi.org\/10.1111\/os.12873.","journal-title":"Orthop Surg"},{"issue":"6","key":"5840_CR5","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.1016\/j.biomaterials.2009.10.059","volume":"31","author":"JM Kanczler","year":"2010","unstructured":"Kanczler JM, Ginty PJ, White L, Clarke NM, Howdle SM, Shakesheff KM, Oreffo RO. The effect of the delivery of vascular endothelial growth factor and bone morphogenic protein-2 to osteoprogenitor cell populations on bone formation. Biomaterials. 2010;31(6):1242\u201350. https:\/\/doi.org\/10.1016\/j.biomaterials.2009.10.059.","journal-title":"Biomaterials"},{"issue":"11","key":"5840_CR6","doi-asserted-by":"publisher","first-page":"111022","DOI":"10.1016\/j.injury.2023.111022","volume":"54","author":"K Wang","year":"2023","unstructured":"Wang K, Gao F, Zhang Y, Dai B, Yan X, He X, Mao D, Rui Y. Comparison of osteogenic activity from different parts of induced membrane in the Masquelet technique. Injury. 2023;54(11):111022. https:\/\/doi.org\/10.1016\/j.injury.2023.111022.","journal-title":"Injury"},{"key":"5840_CR7","doi-asserted-by":"publisher","unstructured":"Klaue K, Knothe U, Anton C, Pfluger DH, Stoddart M, Masquelet AC, Perren SM. Bone regeneration in long-bone defects: tissue compartmentalisation? In vivo study on bone defects in sheep. Injury. 2009;40(4). https:\/\/doi.org\/10.1016\/j.injury.2009.10.043. S95-102.","DOI":"10.1016\/j.injury.2009.10.043"},{"issue":"5","key":"5840_CR8","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1097\/BOT.0b013e3182691288","volume":"27","author":"HE Gruber","year":"2013","unstructured":"Gruber HE, Gettys FK, Montijo HE, Starman JS, Bayoumi E, Nelson KJ, Hoelscher GL, Ramp WK, Zinchenko N, Ingram JA, Bosse MJ, Kellam JF. Genomewide molecular and biologic characterization of biomembrane formation adjacent to a methacrylate spacer in the rat femoral segmental defect model. J Orthop Trauma. 2013;27(5):290\u20137. https:\/\/doi.org\/10.1097\/BOT.0b013e3182691288.","journal-title":"J Orthop Trauma"},{"issue":"1","key":"5840_CR9","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1186\/s12891-023-06740-z","volume":"4","author":"Q Yin","year":"2023","unstructured":"Yin Q, Chen X, Dai B, Liu J, Yang Y, Song S, Ding Y. Varying degrees of spontaneous osteogenesis of Masquelet\u2019s induced membrane: experimental and clinical observations. BMC Musculoskelet Disord. 2023;4(1):384. https:\/\/doi.org\/10.1186\/s12891-023-06740-z.","journal-title":"BMC Musculoskelet Disord"},{"issue":"7","key":"5840_CR10","first-page":"386","volume":"98","author":"MN Magin","year":"1995","unstructured":"Magin MN, Aymar M. Orthotopic tibiofibular fusion: indications\u2013technique\u2013results. Unfallchirurg. 1995;98(7):386\u201391. German.","journal-title":"Unfallchirurg"},{"issue":"6","key":"5840_CR11","first-page":"741","volume":"39","author":"S Piatkowski","year":"1974","unstructured":"Piatkowski S, Modrzewski K. Tibio-fibular fusion in the treatment of infected and difficult tibial pseudarthroses. Chir Narzadow Ruchu Ortop Pol. 1974;39(6):741\u20137. Polish.","journal-title":"Chir Narzadow Ruchu Ortop Pol"},{"key":"5840_CR12","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.asmart.2021.05.002","volume":"25","author":"SK Lim","year":"2021","unstructured":"Lim SK, Ho YC, Ling SK, Yung PS. Functional outcome of fusion versus ligament reconstruction in patients with a syndesmosis injury: a narrative review. Asia Pac J Sports Med Arthrosc Rehabil Technol. 2021;25:53\u20139. https:\/\/doi.org\/10.1016\/j.asmart.2021.05.002.","journal-title":"Asia Pac J Sports Med Arthrosc Rehabil Technol"},{"issue":"1S","key":"5840_CR13","doi-asserted-by":"publisher","first-page":"102778","DOI":"10.1016\/j.otsr.2020.102778","volume":"107","author":"O Cornu","year":"2021","unstructured":"Cornu O, Manon J, Tribak K, Putineanu D. Traumatic injuries of the distal tibiofibular syndesmosis. Orthop Traumatol Surg Res. 2021;107(1S):102778. https:\/\/doi.org\/10.1016\/j.otsr.2020.102778.","journal-title":"Orthop Traumatol Surg Res"},{"issue":"10","key":"5840_CR14","doi-asserted-by":"publisher","first-page":"E382","DOI":"10.1002\/term.1826","volume":"10","author":"D Henrich","year":"2016","unstructured":"Henrich D, Seebach C, Nau C, Basan S, Relja B, Wilhelm K, Schaible A, Frank J, Barker J, Marzi I. Establishment and characterization of the Masquelet induced membrane technique in a rat femur critical-sized defect model. J Tissue Eng Regen Med. 2016;10(10):E382\u2013396. https:\/\/doi.org\/10.1002\/term.1826.","journal-title":"J Tissue Eng Regen Med"},{"issue":"2","key":"5840_CR15","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1097\/00005131-200002000-00002","volume":"14","author":"D Paley","year":"2000","unstructured":"Paley D, Maar DC. Ilizarov bone transport treatment for tibial defects. J Orthop Trauma. 2000;14(2):76\u201385. https:\/\/doi.org\/10.1097\/00005131-200002000-00002.","journal-title":"J Orthop Trauma"},{"issue":"9","key":"5840_CR16","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1007\/s00113-020-00843-1","volume":"123","author":"M Miska","year":"2020","unstructured":"Miska M, Schmidmaier G. Diamond concept for treatment of nonunions and bone defects. Unfallchirurg. 2020;123(9):679\u201386. https:\/\/doi.org\/0.1007\/s00113-020-00843-1.","journal-title":"Unfallchirurg"},{"issue":"9","key":"5840_CR17","doi-asserted-by":"publisher","first-page":"1937","DOI":"10.1007\/s00264-022-05494-y","volume":"46","author":"A Khaled","year":"2022","unstructured":"Khaled A, El-Gebaly O, El-Rosasy M. Masquelet-Ilizarov technique for the management of bone loss post debridement of infected tibial nonunion. Int Orthop. 2022;46(9):1937\u201344. https:\/\/doi.org\/10.1007\/s00264-022-05494-y.","journal-title":"Int Orthop"},{"key":"5840_CR18","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.ijsu.2017.04.064","volume":"42","author":"W Han","year":"2017","unstructured":"Han W, Shen J, Wu H, Yu S, Fu J, Xie Z. Induced membrane technique: advances in the management of bone defects. Int J Surg. 2017;42:110\u20136. https:\/\/doi.org\/10.1016\/j.ijsu.2017.04.064.","journal-title":"Int J Surg"},{"issue":"1","key":"5840_CR19","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.anplas.2008.07.001","volume":"54","author":"P P\u00e9lissier","year":"2009","unstructured":"P\u00e9lissier P, Lefevre Y, Delmond S, Villars F, Vilamitjana-Amedee J. Influences of induced membranes on heterotopic bone formation within an osteo-inductive complex. Experimental study in rabbits. Ann Chir Plast Esthet. 2009;54(1):16\u201320. https:\/\/doi.org\/10.1016\/j.anplas.2008.07.001. French.","journal-title":"Ann Chir Plast Esthet"},{"issue":"2","key":"5840_CR20","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1002\/jor.20738","volume":"27","author":"S Catros","year":"2009","unstructured":"Catros S, Zwetyenga N, Bareille R, Brouillaud B, Renard M, Am\u00e9d\u00e9e J, Fricain JC. Subcutaneous-induced membranes have no osteoinductive effect on macroporous HA-TCP in vivo. J Orthop Res. 2009;27(2):155\u201361. https:\/\/doi.org\/10.1002\/jor.20738.","journal-title":"J Orthop Res"},{"issue":"3","key":"5840_CR21","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1302\/2046-3758.83.BJR-2018-0096.R1","volume":"8","author":"ZXH Lim","year":"2019","unstructured":"Lim ZXH, Rai B, Tan TC, Ramruttun AK, Hui JH, Nurcombe V, Teoh SH, Cool SM. Autologous bone marrow clot as an alternative to autograft for bone defect healing. Bone Joint Res. 2019;8(3):107\u201317. https:\/\/doi.org\/10.1302\/2046-3758.83.BJR-2018-0096.R1.","journal-title":"Bone Joint Res"},{"issue":"4","key":"5840_CR22","doi-asserted-by":"publisher","first-page":"2723","DOI":"10.1002\/jcp.26065","volume":"233","author":"F Salamanna","year":"2018","unstructured":"Salamanna F, Contartese D, Nicoli Aldini N, Barbanti Brodano G, Griffoni C, Gasbarrini A, Fini M. Bone marrow aspirate clot: a technical complication or a smart approach for musculoskeletal tissue regeneration? J Cell Physiol. 2018;233(4):2723\u2013232. https:\/\/doi.org\/10.1002\/jcp.26065.","journal-title":"J Cell Physiol"},{"key":"5840_CR23","doi-asserted-by":"publisher","first-page":"1280332","DOI":"10.3389\/fsurg.2023.1280332","volume":"10","author":"Z Liu","year":"2023","unstructured":"Liu Z, Liu Q, Wang M, Zhou C, Guo H, Liang J, Zhang Y. The early posterior cortex pixel value ratio: a novel reliable indicator for distraction osteogenesis. Front Surg. 2023;10:1280332. https:\/\/doi.org\/10.3389\/fsurg.2023.1280332.","journal-title":"Front Surg"},{"issue":"1","key":"5840_CR24","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1186\/s12891-021-04317-2","volume":"22","author":"Y Lu","year":"2021","unstructured":"Lu Y, Wang J, Yang Y, Yin Q. Bone defects are repaired by enhanced osteogenic activity of the induced membrane: a case report and literature review. BMC Musculoskelet Disord. 2021;22(1):447. https:\/\/doi.org\/10.1186\/s12891-021-04317-2.","journal-title":"BMC Musculoskelet Disord"},{"issue":"1","key":"5840_CR25","doi-asserted-by":"publisher","first-page":"e016","DOI":"10.1097\/OI9.0000000000000016","volume":"2","author":"K Tetsworth","year":"2019","unstructured":"Tetsworth K, Woloszyk A, Glatt V. 3D printed titanium cages combined with the Masquelet technique for the reconstruction of segmental femoral defects: preliminary clinical results and molecular analysis of the biological activity of human-induced membranes. OTA Int. 2019;2(1):e016. https:\/\/doi.org\/10.1097\/OI9.0000000000000016.","journal-title":"OTA Int"},{"issue":"14","key":"5840_CR26","doi-asserted-by":"publisher","first-page":"2728","DOI":"10.1016\/j.jbiomech.2010.06.026","volume":"43","author":"UR Knothe","year":"2010","unstructured":"Knothe UR, Dolejs S, Matthew Miller R, Knothe Tate ML. Effects of mechanical loading patterns, bone graft, and proximity to periosteum on bone defect healing. J Biomech. 2010;43(14):2728\u201337. https:\/\/doi.org\/10.1016\/j.jbiomech.2010.06.026.","journal-title":"J Biomech"},{"issue":"6","key":"5840_CR27","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1097\/BPO.0b013e318221ebce","volume":"31","author":"IH Choi","year":"2011","unstructured":"Choi IH, Lee SJ, Moon HJ, Cho TJ, Yoo WJ, Chung CY, Park MS. 4-in-1 osteosynthesis for atrophic-type congenital pseudarthrosis of the tibia. J Pediatr Orthop. 2011;31(6):697\u2013704. https:\/\/doi.org\/10.1097\/BPO.0b013e318221ebce.","journal-title":"J Pediatr Orthop"},{"issue":"3","key":"5840_CR28","first-page":"346","volume":"45","author":"AC Masquelet","year":"2000","unstructured":"Masquelet AC, Fitoussi F, Begue T, Muller GP. Reconstruction of the long bones by the induced membrane and spongy autograft. Ann Chir Plast Esthet. 2000;45(3):346\u201353. French.","journal-title":"Ann Chir Plast Esthet"},{"issue":"4","key":"5840_CR29","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1097\/00009957-200210000-00006","volume":"11","author":"KY Yang","year":"2002","unstructured":"Yang KY, Lee EH. Isolated congenital pseudoarthrosis of the fibula. J Pediatr Orthop B. 2002;11(4):298\u2013301. https:\/\/doi.org\/10.1097\/00009957-200210000-00006.","journal-title":"J Pediatr Orthop B"},{"issue":"3","key":"5840_CR30","doi-asserted-by":"publisher","first-page":"290","DOI":"10.4103\/0019-5413.181780","volume":"50","author":"G Gupta","year":"2016","unstructured":"Gupta G, Ahmad S, Mohd Zahid, Khan AH, Sherwani MK, Khan AQ. Management of traumatic tibial diaphyseal bone defect by induced-membrane technique. Indian J Orthop. 2016;50(3):290\u20136. https:\/\/doi.org\/10.4103\/0019-5413.181780.","journal-title":"Indian J Orthop"}],"container-title":["Journal of Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-024-05840-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12967-024-05840-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-024-05840-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T12:49:29Z","timestamp":1740746969000},"score":1,"resource":{"primary":{"URL":"https:\/\/translational-medicine.biomedcentral.com\/articles\/10.1186\/s12967-024-05840-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["5840"],"URL":"https:\/\/doi.org\/10.1186\/s12967-024-05840-1","relation":{},"ISSN":["1479-5876"],"issn-type":[{"value":"1479-5876","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]},"assertion":[{"value":"27 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This experimental study was reviewed and approved by the Institutional Animal Care and Use Committee of Wuxi Ninth People\u2019s Hospital (No. JY-KT20220132, registration date: October 10, 2022). We have adhered to the ARRIVE guidelines and have included the ARRIVE checklist. Our retrospective clinical study received approval from the Review Board of Wuxi Ninth Peopls\u2019s Hospital (No. JY-KT2024067, registration date: March 15, 2024) and was performed in accordance with the ethical standards outlined in the 1964 Declaration of Helsinki and its subsequent amendments. Written informed consent for participation in the study was obtained from each patient.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consents of publication of any potentially identifiable images or data included in the study were obtained from the individuals (date: July 2024).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors have no conflicts of interest to disclose. All authors have.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"read and approved the final submitted manuscript.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"239"}}