{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T09:37:24Z","timestamp":1767433044792,"version":"3.48.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T00:00:00Z","timestamp":1767398400000},"content-version":"vor","delay-in-days":37,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"DOI":"10.1186\/s12967-025-07481-4","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:08:09Z","timestamp":1764259689000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Double vitrification-warming cycles coupled with one blastocyst biopsy do not affect the chance of live birth after an euploid blastocyst transfer: a propensity score matching study"],"prefix":"10.1186","volume":"24","author":[{"given":"Xin","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyan","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"7481_CR1","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1016\/j.fertnstert.2013.04.035","volume":"1003","author":"UKM Scott R T Jr.","year":"2013","unstructured":"Scott R T UKM Jr., J FE, H HK, L SK, Taylor D, et al. Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial. Fertil Steril. 2013;1003:697\u2013703.","journal-title":"Fertil Steril"},{"key":"7481_CR2","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1093\/humrep\/deu033","volume":"296","author":"A Capalbo","year":"2014","unstructured":"Capalbo A, Rienzi L, Cimadomo D, Maggiulli R, Elliott T, Wright G, et al. Correlation between standard blastocyst morphology, euploidy and implantation: an observational study in two centers involving 956 screened blastocysts. Hum Reprod. 2014;296:1173\u201381.","journal-title":"Hum Reprod"},{"key":"7481_CR3","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1007\/s10815-016-0785-2","volume":"3310","author":"A Vaiarelli","year":"2016","unstructured":"Vaiarelli A, Cimadomo D, Capalbo A, Orlando G, Sapienza F, Colamaria S, et al. Pre-implantation genetic testing in art: who will benefit and what is the evidence? J Assist Reprod Genet. 2016;3310:1273\u201378.","journal-title":"J Assist Reprod Genet"},{"key":"7481_CR4","first-page":"421","volume":"1223","author":"The use of preimplantation genetic testing for aneuploidy: a committee opinion","year":"2024","unstructured":"The use of preimplantation genetic testing for aneuploidy: a committee opinion. Fertil Steril. 2024;1223:421\u201334.","journal-title":"Fertil Steril"},{"key":"7481_CR5","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/j.fertnstert.2012.05.025","volume":"983","author":"B Hodes-Wertz","year":"2012","unstructured":"Hodes-Wertz B, Grifo J, Ghadir S, Kaplan B, A LC, Glassner M, et al. Idiopathic recurrent miscarriage is caused mostly by aneuploid embryos. Fertil Steril. 2012;983:675\u201380.","journal-title":"Fertil Steril"},{"key":"7481_CR6","doi-asserted-by":"publisher","first-page":"103738","DOI":"10.1016\/j.rbmo.2023.103738","volume":"491","author":"WH Kutteh","year":"2024","unstructured":"Kutteh WH, Papas RS, Maisenbacher MK, Dahdouh EM. Role of genetic analysis of products of conception and PGT in managing early pregnancy loss. Reprod Biomed Online. 2024;491:103738.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR7","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1093\/humrep\/dez289","volume":"351","author":"T Sato","year":"2020","unstructured":"Sato T, Sugiura-Ogasawara M, Ozawa F, Yamamoto T, Kato T, Kurahashi H, et al. Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure. Hum Reprod. 2020;351:255.","journal-title":"Hum Reprod"},{"key":"7481_CR8","doi-asserted-by":"publisher","first-page":"2339","DOI":"10.1093\/humrep\/deab117","volume":"368","author":"SJ Bhatt","year":"2021","unstructured":"Bhatt SJ, Marchetto NM, Roy J, Morelli SS, McGovern PG. Pregnancy outcomes following in vitro fertilization frozen embryo transfer (IVF-FET) with or without preimplantation genetic testing for aneuploidy (PGT-A) in women with recurrent pregnancy loss (RPL): a SART-CORS study. Hum Reprod. 2021;368:2339\u201344.","journal-title":"Hum Reprod"},{"key":"7481_CR9","doi-asserted-by":"crossref","unstructured":"Mumusoglu S, Telek SB, Ata B. Preimplantation genetic testing for aneuploidy in unexplained recurrent pregnancy loss: a systematic review and meta-analysis. Fertil Steril. 2024.","DOI":"10.1016\/j.fertnstert.2024.07.715"},{"key":"7481_CR10","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.rbmo.2014.03.001","volume":"291","author":"TH Taylor","year":"2014","unstructured":"Taylor TH, Patrick JL, Gitlin SA, Michael Wilson J, Crain JL, Griffin DK. Outcomes of blastocysts biopsied and vitrified once versus those cryopreserved twice for euploid blastocyst transfer. Reprod Biomed Online. 2014;291:59\u201364.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR11","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1016\/j.fertnstert.2017.09.013","volume":"1086","author":"CK Bradley","year":"2017","unstructured":"Bradley CK, Livingstone M, Traversa MV, McArthur SJ. Impact of multiple blastocyst biopsy and vitrification-warming procedures on pregnancy outcomes. Fertil Steril. 2017;1086:999\u20131006.","journal-title":"Fertil Steril"},{"key":"7481_CR12","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1002\/ijgo.14355","volume":"1603","author":"X Li","year":"2023","unstructured":"Li X, Li W, Jia H, Gao Y, Shi W, Bai H. Double vitrification-warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes. Int J Gynaecol Obstet. 2023;1603:806\u201313.","journal-title":"Int J Gynaecol Obstet"},{"key":"7481_CR13","first-page":"hoad002","volume":"20231","author":"R Bender Atik","year":"2023","unstructured":"Bender Atik R, B CO, Elson J, M KA, Lewis S, Middeldorp S, et al. Eshre guideline: recurrent pregnancy loss: an update in 2022. Hum Reprod Open. 2023;20231:hoad002.","journal-title":"Hum Reprod Open"},{"key":"7481_CR14","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.rbmo.2013.08.011","volume":"281","author":"C Coughlan","year":"2014","unstructured":"Coughlan C, Ledger W, Wang Q, Liu F, Demirol A, Gurgan T, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online. 2014;281:14\u201338.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR15","doi-asserted-by":"publisher","first-page":"1082597","DOI":"10.3389\/fendo.2022.1082597","volume":"13","author":"X Liu","year":"2022","unstructured":"Liu X, Lou H, Zhang J, Du M, Du Y, Wu S, et al. Clinical outcome analysis of frozen-thawed embryo transfer on day 7. Front Endocrinol (Lausanne). 2022;13:1082597.","journal-title":"Front Endocrinol (lausanne)"},{"key":"7481_CR16","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1186\/s12884-020-03082-9","volume":"201","author":"W Niu","year":"2020","unstructured":"Niu W, Wang L, Xu J, Li Y, Shi H, Li G, et al. Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array. BMC Pregnancy Childbirth. 2020;201:388.","journal-title":"BMC Pregnancy Childbirth"},{"key":"7481_CR17","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1016\/j.fertnstert.2014.01.051","volume":"1015","author":"F Fiorentino","year":"2014","unstructured":"Fiorentino F, Biricik A, Bono S, Spizzichino L, Cotroneo E, Cottone G, et al. Development and validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of embryos. Fertil Steril. 2014;1015:1375\u201382.","journal-title":"Fertil Steril"},{"key":"7481_CR18","doi-asserted-by":"crossref","unstructured":"Vera-Rodr\u00edguez M, Michel C E, Mercader A, Bladon A J, Rodrigo L, Kokocinski F, et al. Distribution patterns of segmental aneuploidies in human blastocysts identified by next-generation sequencing. Fertil Steril. 2016; 1054:1047\u20131055.e1042.","DOI":"10.1016\/j.fertnstert.2015.12.022"},{"key":"7481_CR19","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.tjog.2023.10.003","volume":"631","author":"SS Bahreiny","year":"2024","unstructured":"Bahreiny SS, Bastani MN, Aghaei M, Dabbagh MR, Mahdizade AH. Circulating galectin-3 levels in women with polycystic ovary syndrome: a meta-analysis. Taiwan J Obstet Gynecol. 2024;631:37\u201345.","journal-title":"Taiwan J Obstet Gynecol"},{"key":"7481_CR20","doi-asserted-by":"publisher","first-page":"2957","DOI":"10.1007\/s43032-024-01676-8","volume":"3110","author":"SS Bahreiny","year":"2024","unstructured":"Bahreiny SS, Ahangarpour A, Rajaei E, Sharifani MS, Aghaei M. Meta-analytical and meta-regression evaluation of subclinical Hyperthyroidism\u2019s effect on male reproductive health: hormonal and seminal perspectives. Reprod Sci. 2024;3110:2957\u201371.","journal-title":"Reprod Sci"},{"key":"7481_CR21","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1093\/humrep\/dead042","volume":"385","author":"F Rafael","year":"2023","unstructured":"Rafael F, D RM, Bellver J, Canelas-Pais M, Garrido N, A G-VJ, et al. The combined effect of bmi and age on art outcomes. Hum Reprod. 2023;385:886\u201394.","journal-title":"Hum Reprod"},{"key":"7481_CR22","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1016\/j.fertnstert.2023.08.953","volume":"1206","author":"N Vyas","year":"2023","unstructured":"Vyas N, Adjei N, Spandorfer SD. Euploid programmed frozen embryo transfer cycles are associated with a higher live birth rate when estradiol levels more closely mimic physiology. Fertil Steril. 2023;1206:1220\u201326.","journal-title":"Fertil Steril"},{"key":"7481_CR23","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1097\/RD9.0000000000000089","volume":"8","author":"SS Bahreiny","year":"2024","unstructured":"Bahreiny SS, Ahangarpour A, Aghaei M. Circulating levels of advanced glycation end products in females with polycystic ovary syndrome: a meta-analysis. Reprod And Dev Med. 2024;8:93\u2013100.","journal-title":"Reprod And Dev Med"},{"key":"7481_CR24","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/j.fertnstert.2013.04.039","volume":"1003","author":"UKM Scott R T Jr.","year":"2013","unstructured":"Scott R T, Jr., Upham K M, Forman E J, Zhao T, Treff N R. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril. 2013;1003:624\u201330.","journal-title":"Fertil Steril"},{"key":"7481_CR25","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1093\/humrep\/del506","volume":"225","author":"G Kokkali","year":"2007","unstructured":"Kokkali G, Traeger-Synodinos J, Vrettou C, Stavrou D, M JG, S CD, et al. Blastocyst biopsy versus cleavage stage biopsy and blastocyst transfer for preimplantation genetic diagnosis of beta-thalassaemia: a pilot study. Hum Reprod. 2007;225:1443\u201349.","journal-title":"Hum Reprod"},{"key":"7481_CR26","doi-asserted-by":"publisher","first-page":"2333","DOI":"10.1007\/s10815-023-02916-7","volume":"4010","author":"S Zhang","year":"2023","unstructured":"Zhang S, Xie P, Lan F, Yao Y, Ma S, Hu L, et al. Conventional IVF is feasible in preimplantation genetic testing for aneuploidy. J Assist Reprod Genet. 2023;4010:2333\u201342.","journal-title":"J Assist Reprod Genet"},{"key":"7481_CR27","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.rbmo.2022.08.103","volume":"461","author":"Y Dong","year":"2023","unstructured":"Dong Y, Liu D, Zou Y, Wan C, Chen C, Dong M, et al. Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study. Reprod Biomed Online. 2023;461:69\u201379.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR28","doi-asserted-by":"publisher","first-page":"2302","DOI":"10.1007\/s43032-023-01177-0","volume":"307","author":"Q Zhang","year":"2023","unstructured":"Zhang Q, Yu W, Jin C, Ni T, Zhou T, Zhao Q, et al. Impact of multiple vitrification-warming procedures and insemination methods on pregnancy and neonatal outcomes in preimplantation genetic testing for aneuploidy. Reprod Sci. 2023;307:2302\u201312.","journal-title":"Reprod Sci"},{"key":"7481_CR29","first-page":"223","volume":"113","author":"M Wilding","year":"2019","unstructured":"Wilding M, Terribile M, Parisi I, Nargund G. Thaw, biopsy and refreeze strategy for PGT-A on previously cryopreserved embryos. Facts Views Vis Obgyn. 2019;113:223\u201327.","journal-title":"Facts Views Vis Obgyn"},{"key":"7481_CR30","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/j.rbmo.2021.06.028","volume":"434","author":"M Hallamaa","year":"2021","unstructured":"Hallamaa M, Seikkula J, Willman S, Ollila H, Jokimaa V. Pregnancy potential and perinatal outcomes of embryos cryopreserved twice: a case-control study. Reprod Biomed Online. 2021;434:607\u201313.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR31","doi-asserted-by":"publisher","first-page":"2357","DOI":"10.1007\/s10815-023-02909-6","volume":"4010","author":"L Nanassy","year":"2023","unstructured":"Nanassy L, Schoepper B, Schultze-Mosgau A, Depenbusch M, K ET, F HRA, et al. Evaluation of live birth rates and perinatal outcomes following two sequential vitrification\/warming events at the zygote and blastocyst stages. J Assist Reprod Genet. 2023;4010:2357\u201365.","journal-title":"J Assist Reprod Genet"},{"key":"7481_CR32","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1016\/j.rbmo.2020.11.019","volume":"423","author":"A Aluko","year":"2021","unstructured":"Aluko A, Vaughan D A, Modest A M, Penzias A S, Hacker M R, Thornton K, et al. Multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes. Reprod Biomed Online. 2021;423:572\u201378.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR33","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.rbmo.2020.11.007","volume":"421","author":"M Wang","year":"2021","unstructured":"Wang M, Jiang J, Xi Q, Li D, Ren X, Li Z, et al. Repeated cryopreservation process impairs embryo implantation potential but does not affect neonatal outcomes. Reprod Biomed Online. 2021;421:75\u201382.","journal-title":"Reprod Biomed Online"},{"key":"7481_CR34","first-page":"896","volume":"1105","author":"NS A","year":"2018","unstructured":"Neal S A, Morin S J, Franasiak J M, Goodman L R, Juneau C R, Forman E J, et al. Preimplantation genetic testing for aneuploidy is cost-effective, shortens treatment time, and reduces the risk of failed embryo transfer and clinical miscarriage. Fertil Steril. 2018;1105:896\u2013904.","journal-title":"Fertil Steril"},{"key":"7481_CR35","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1016\/j.fertnstert.2019.01.025","volume":"1116","author":"E Somigliana","year":"2019","unstructured":"Somigliana E, Busnelli A, Paffoni A, Vigano P, Riccaboni A, Rubio C, et al. Cost-effectiveness of preimplantation genetic testing for aneuploidies. Fertil Steril. 2019;1116:1169\u201376.","journal-title":"Fertil Steril"},{"key":"7481_CR36","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s12958-017-0269-y","volume":"151","author":"PN Scriven","year":"2017","unstructured":"Scriven PN. Towards a better understanding of preimplantation genetic screening for aneuploidy: insights from a virtual trial for women under the age of 40 when transferring embryos one at a time. Reprod Biol Endocrinol. 2017;151:49.","journal-title":"Reprod Biol Endocrinol"}],"container-title":["Journal of Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-025-07481-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12967-025-07481-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-025-07481-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T09:34:26Z","timestamp":1767432866000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12967-025-07481-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["7481"],"URL":"https:\/\/doi.org\/10.1186\/s12967-025-07481-4","relation":{},"ISSN":["1479-5876"],"issn-type":[{"type":"electronic","value":"1479-5876"}],"subject":[],"published":{"date-parts":[[2025,11,27]]},"assertion":[{"value":"18 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"None declared.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"16"}}