{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:31:50Z","timestamp":1791613910453,"version":"4.3.4"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":32,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"DOI":"10.1186\/s12967-026-07762-6","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T17:14:58Z","timestamp":1770657298000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular residual disease assessment in colorectal and bladder cancer by somatic structural variant analysis of cell-free DNA whole-genome sequencing data"],"prefix":"10.1186","volume":"24","author":[{"given":"Ester Ellegaard","family":"S\u00f8rensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Frydendahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads Heilskov","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iver","family":"Nordentoft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Knudsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenna Vesterman","family":"Henriksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sia Viborg","family":"Lindskrog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Dyrskj\u00f8t","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7406-2103","authenticated-orcid":false,"given":"Claus Lindbjerg","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper Bertram","family":"Bramsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,9]]},"reference":[{"issue":"4","key":"7762_CR1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/nrc.2017.7","volume":"17","author":"JCM Wan","year":"2017","unstructured":"Wan JCM, Massie C, Garcia-Corbacho J, Mouliere F, Brenton JD, Caldas C, et al. Liquid biopsies come of age: towards implementation of circulating tumour DNA. Nat Rev Cancer. 2017;17(4):223\u201338.","journal-title":"Nat Rev Cancer"},{"issue":"6","key":"7762_CR2","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1038\/s41591-024-03040-4","volume":"30","author":"AJ Widman","year":"2024","unstructured":"Widman AJ, Shah M, Frydendahl A, Halmos D, Khamnei CC, \u00d8gaard N, et al. Ultrasensitive plasma-based monitoring of tumor burden using machine-learning-guided signal enrichment. Nat Med. 2024;30(6):1655\u201366.","journal-title":"Nat Med"},{"issue":"7","key":"7762_CR3","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.1038\/s41591-020-0915-3","volume":"26","author":"A Zviran","year":"2020","unstructured":"Zviran A, Schulman RC, Shah M, Hill STK, Deochand S, Khamnei CC, et al. Genome-wide cell-free DNA mutational integration enables ultra-sensitive cancer monitoring. Nat Med. 2020;26(7):1114\u201324.","journal-title":"Nat Med"},{"key":"7762_CR4","doi-asserted-by":"crossref","unstructured":"Nordentoft I, Lindskrog SV, Birkenkamp-Demtr\u00f6der K, Gonzalez S, Kuzman M, Levatic J, et al. Whole-genome mutational analysis for tumor-informed detection of circulating tumor DNA in patients with urothelial carcinoma. Eur Urol. 2024.","DOI":"10.1016\/j.eururo.2024.05.014"},{"issue":"3","key":"7762_CR5","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1158\/1078-0432.CCR-21-2404","volume":"28","author":"TV Henriksen","year":"2022","unstructured":"Henriksen TV, Tarazona N, Frydendahl A, Reinert T, Gimeno-Valiente F, Carbonell-Asins JA, et al. Circulating tumor DNA in stage III colorectal cancer, beyond minimal residual disease detection, toward assessment of adjuvant Therapy efficacy and clinical behavior of recurrences. Clin Cancer Res. 2022;28(3):507\u201317.","journal-title":"Clin Cancer Res"},{"key":"7762_CR6","doi-asserted-by":"crossref","unstructured":"Frydendahl A, Nors J, Rasmussen MH, Henriksen TV, Nesic M, Reinert T, et al. Detection of circulating tumor DNA by tumor-informed whole-genome sequencing enables prediction of recurrence in stage III colorectal cancer patients. Eur J Cancer. 2024;114314.","DOI":"10.1016\/j.ejca.2024.114314"},{"key":"7762_CR7","doi-asserted-by":"publisher","first-page":"S1294","DOI":"10.1016\/j.annonc.2023.10.049","volume":"34","author":"JRM Black","year":"2023","unstructured":"Black JRM, Frankell AM, Veeriah S, Colopi M, Hill M, Abbott CW, et al. LBA55 an ultra-sensitive and specific ctDNA assay provides novel pre-operative disease stratification in early stage lung cancer. Ann Oncol. 2023;34:S1294.","journal-title":"Ann Oncol"},{"issue":"5","key":"7762_CR8","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1038\/nm.3519","volume":"20","author":"AM Newman","year":"2014","unstructured":"Newman AM, Bratman SV, To J, Wynne JF, Eclov NCW, Modlin LA, et al. An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage. Nat Med. 2014;20(5):548\u201354.","journal-title":"Nat Med"},{"key":"7762_CR9","doi-asserted-by":"crossref","unstructured":"Frydendahl A, Rasmussen MH, Jensen S, Henriksen TV, Demuth C, Diekema M, et al. Error-corrected deep targeted sequencing of circulating cell-free DNA from colorectal cancer patients for sensitive detection of circulating tumor DNA. Int J Mol Sci. 2024;25(8).","DOI":"10.3390\/ijms25084252"},{"key":"7762_CR10","doi-asserted-by":"crossref","unstructured":"Wan JCM, Heider K, Gale D, Murphy S, Fisher E, Mouliere F, et al. ctDNA monitoring using patient-specific sequencing and integration of variant reads. Sci Transl Med. 2020;12(548).","DOI":"10.1126\/scitranslmed.aaz8084"},{"issue":"8","key":"7762_CR11","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1001\/jamaoncol.2019.0528","volume":"5","author":"T Reinert","year":"2019","unstructured":"Reinert T, Henriksen TV, Christensen E, Sharma S, Salari R, Sethi H, et al. Analysis of plasma cell-free DNA by ultradeep sequencing in patients with stages I to III colorectal cancer. JAMA Oncol. 2019;5(8):1124.","journal-title":"JAMA Oncol"},{"issue":"2","key":"7762_CR12","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.annonc.2023.11.009","volume":"35","author":"TV Henriksen","year":"2024","unstructured":"Henriksen TV, Demuth C, Frydendahl A, Nors J, Nesic M, Rasmussen MH, et al. Unraveling the potential clinical utility of circulating tumor DNA detection in colorectal cancer-evaluation in a nationwide Danish cohort. Ann Oncol. 2024;35(2):229\u201339.","journal-title":"Ann Oncol"},{"issue":"12","key":"7762_CR13","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.1038\/s41587-021-00981-w","volume":"39","author":"DM Kurtz","year":"2021","unstructured":"Kurtz DM, Soo J, Co Ting Keh L, Alig S, Chabon JJ, Sworder BJ, et al. Enhanced detection of minimal residual disease by targeted sequencing of phased variants in circulating tumor DNA. Nat Biotechnol. 2021;39(12):1537\u201347.","journal-title":"Nat Biotechnol"},{"key":"7762_CR14","doi-asserted-by":"crossref","unstructured":"Nesic M, Rasmussen MH, Henriksen TV, Demuth C, Frydendahl A, Nordentoft I, et al. Beyond basics: key mutation selection features for successful tumor-informed ctDNA detection. Int J Cancer. 2024.","DOI":"10.1002\/ijc.34964"},{"issue":"7793","key":"7762_CR15","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/s41586-019-1913-9","volume":"578","author":"Y Li","year":"2020","unstructured":"Li Y, Roberts ND, Wala JA, Shapira O, Schumacher SE, Kumar K, et al. Patterns of somatic structural variation in human cancer genomes. Nature. 2020;578(7793):112\u201321.","journal-title":"Nature"},{"issue":"7793","key":"7762_CR16","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1038\/s41586-020-1969-6","volume":"578","author":"LA Aaltonen","year":"2020","unstructured":"Aaltonen LA, Abascal F, Abeshouse A, Aburatani H, Adams DJ, Agrawal N, et al. Pan-cancer analysis of whole genomes. Nature. 2020;578(7793):82\u201393.","journal-title":"Nature"},{"issue":"8028","key":"7762_CR17","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1038\/s41586-024-07747-9","volume":"633","author":"AJ Cornish","year":"2024","unstructured":"Cornish AJ, Gruber AJ, Kinnersley B, Chubb D, Frangou A, Caravagna G, et al. The genomic landscape of 2, 023 colorectal cancers. Nature. 2024;633(8028):127\u201336.","journal-title":"Nature"},{"issue":"20","key":"7762_CR18","doi-asserted-by":"publisher","first-page":"20ra14","DOI":"10.1126\/scitranslmed.3000702","volume":"2","author":"RJ Leary","year":"2010","unstructured":"Leary RJ, Kinde I, Diehl F, Schmidt K, Clouser C, Duncan C, et al. Development of personalized tumor biomarkers using massively parallel sequencing. Sci Transl Med. 2010;2(20):20ra14.","journal-title":"Sci Transl Med"},{"issue":"3","key":"7762_CR19","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1038\/s41576-019-0180-9","volume":"21","author":"SS Ho","year":"2020","unstructured":"Ho SS, Urban AE, Mills RE. Structural variation in the sequencing era. Nat Rev Genet. 2020;21(3):171\u201389.","journal-title":"Nat Rev Genet"},{"issue":"4","key":"7762_CR20","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1136\/gutjnl-2014-308859","volume":"65","author":"T Reinert","year":"2016","unstructured":"Reinert T, Sch\u00f8ler LV, Thomsen R, Tobiasen H, Vang S, Nordentoft I, et al. Analysis of circulating tumour DNA to monitor disease burden following colorectal cancer surgery. Gut. 2016;65(4):625.","journal-title":"Gut"},{"key":"7762_CR21","doi-asserted-by":"crossref","unstructured":"Elliott MJ, Howarth K, Main S, Fuentes Antr\u00e1s J, Echelard P, Dou A, et al. Ultrasensitive detection and monitoring of circulating tumor DNA using structural variants in early-stage breast cancer. Clin Cancer Res. 2025;OF1\u201313.","DOI":"10.1158\/1078-0432.29052829"},{"issue":"6","key":"7762_CR22","doi-asserted-by":"publisher","first-page":"e16505","DOI":"10.15252\/emmm.202216505","volume":"15","author":"A Santonja","year":"2023","unstructured":"Santonja A, Cooper WN, Eldridge MD, Edwards PAW, Morris JA, Edwards AR, et al. Comparison of tumor-informed and tumor-na\u00efve sequencing assays for ctDNA detection in breast cancer. EMBO Mol Med. 2023;15(6):e16505.","journal-title":"EMBO Mol Med"},{"key":"7762_CR23","doi-asserted-by":"crossref","unstructured":"Van Belzen IAEM, Sch\u00f6nhuth, Kemmeren P, Hehir-Kwa JY. Structural variant detection in cancer genomes: computational challenges and perspectives for precision oncology. npj Precis Oncol. 2021;5(1).","DOI":"10.1038\/s41698-021-00155-6"},{"key":"7762_CR24","doi-asserted-by":"crossref","unstructured":"Wei L, Dugas M, Sandmann S. SimFFPE and FilterFFPE: improving structural variant calling in FFPE samples. GigaScience. 2021;10(9).","DOI":"10.1093\/gigascience\/giab065"},{"key":"7762_CR25","doi-asserted-by":"crossref","unstructured":"Gong T, Hayes VM, Chan EKF. Detection of somatic structural variants from short-read next-generation sequencing data. Briefings Bioinf. 2020;22(3).","DOI":"10.1093\/bib\/bbaa056"},{"issue":"8","key":"7762_CR26","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1093\/bioinformatics\/btv710","volume":"32","author":"X Chen","year":"2016","unstructured":"Chen X, Schulz-Trieglaff O, Shaw R, Barnes B, Schlesinger F, K\u00e4llberg M, et al. Manta: rapid detection of structural variants and indels for germline and cancer sequencing applications. Bioinformatics. 2016;32(8):1220\u201322.","journal-title":"Bioinformatics"},{"issue":"18","key":"7762_CR27","doi-asserted-by":"publisher","first-page":"i333","DOI":"10.1093\/bioinformatics\/bts378","volume":"28","author":"T Rausch","year":"2012","unstructured":"Rausch T, Zichner T, Schlattl A, Stutz AM, Benes V, Korbel JO. DELLY: structural variant discovery by integrated paired-end and split-read analysis. Bioinformatics. 2012;28(18):i333\u20139.","journal-title":"Bioinformatics"},{"issue":"7","key":"7762_CR28","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1101\/gr.10.7.967","volume":"10","author":"G T\u00f3th","year":"2000","unstructured":"T\u00f3th G, G\u00e1sp\u00e1ri Z, Jurka J. Microsatellites in different eukaryotic genomes: survey and analysis. Genome Res. 2000;10(7):967\u201381.","journal-title":"Genome Res"},{"issue":"1","key":"7762_CR29","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/s13073-021-00899-7","volume":"13","author":"JE Valle-Inclan","year":"2021","unstructured":"Valle-Inclan JE, Stangl C, de Jong AC, van Dessel LF, van Roosmalen MJ, Helmijr JCA, et al. Optimizing nanopore sequencing-based detection of structural variants enables individualized circulating tumor DNA-based disease monitoring in cancer patients. Genome Med. 2021;13(1):86.","journal-title":"Genome Med"},{"issue":"10","key":"7762_CR30","doi-asserted-by":"publisher","first-page":"2016","DOI":"10.1158\/1535-7163.MCT-20-0987","volume":"20","author":"AT Shah","year":"2021","unstructured":"Shah AT, Azad TD, Breese MR, Chabon JJ, Hamilton EG, Straessler K, et al. A comprehensive circulating tumor DNA assay for detection of translocation and copy-number changes in pediatric sarcomas. Mol Cancer Ther. 2021;20(10):2016\u201325.","journal-title":"Mol Cancer Ther"},{"key":"7762_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1200\/PO.17.00285","volume":"2","author":"K Klega","year":"2018","unstructured":"Klega K, Imamovic-Tuco A, Ha G, Clapp AN, Meyer S, Ward A, et al. Detection of somatic structural variants enables quantification and variants enables quantificationharacterization of circulating tumor DNA in children with solid tumors. JCO Precis Oncol. 2018;2:1\u201313.","journal-title":"JCO Precis Oncol"},{"issue":"1","key":"7762_CR32","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1186\/s13059-019-1828-7","volume":"20","author":"M Mahmoud","year":"2019","unstructured":"Mahmoud M, Gobet N, Cruz-D\u00e1valos DI, Mounier N, Dessimoz C, Sedlazeck FJ. Structural variant calling: the long and the short of it. Genome Biol. 2019;20(1):246.","journal-title":"Genome Biol"},{"issue":"1","key":"7762_CR33","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1038\/s42003-021-02930-4","volume":"4","author":"M Ikegami","year":"2021","unstructured":"Ikegami M, Kohsaka S, Hirose T, Ueno T, Inoue S, Kanomata N, et al. MicroSEC filters sequence errors for formalin-fixed and paraffin-embedded samples. Commun Biol. 2021;4(1):1396.","journal-title":"Commun Biol"},{"issue":"2","key":"7762_CR34","doi-asserted-by":"publisher","first-page":"e12","DOI":"10.1093\/nar\/gky1142","volume":"47","author":"S Haile","year":"2019","unstructured":"Haile S, Corbett RD, Bilobram S, Bye MH, Kirk H, Pandoh P, et al. Sources of erroneous sequences and artifact chimeric reads in next generation sequencing of genomic DNA from formalin-fixed paraffin-embedded samples. Nucleic Acids Res. 2019;47(2):e12.","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"7762_CR35","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1001\/jamaoncol.2019.3616","volume":"5","author":"J Tie","year":"2019","unstructured":"Tie J, Cohen JD, Wang Y, Christie M, Simons K, Lee M, et al. Circulating tumor DNA analyses as markers of recurrence risk and benefit of adjuvant Therapy for stage III colon cancer. JAMA Oncol. 2019;5(12):1710\u201317.","journal-title":"JAMA Oncol"},{"issue":"346","key":"7762_CR36","doi-asserted-by":"publisher","first-page":"346ra92","DOI":"10.1126\/scitranslmed.aaf6219","volume":"8","author":"J Tie","year":"2016","unstructured":"Tie J, Wang Y, Tomasetti C, Li L, Springer S, Kinde I, et al. Circulating tumor DNA analysis detects minimal residual disease and predicts recurrence in patients with stage II colon cancer. Sci Transl Med. 2016;8(346):346ra92.","journal-title":"Sci Transl Med"},{"issue":"7960","key":"7762_CR37","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1038\/s41586-023-05896-x","volume":"617","author":"W-W Liao","year":"2023","unstructured":"Liao W-W, Asri M, Ebler J, Doerr D, Haukness M, Hickey G, et al. A draft human pangenome reference. Nature. 2023;617(7960):312\u201324.","journal-title":"Nature"},{"issue":"6588","key":"7762_CR38","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1126\/science.abj6987","volume":"376","author":"S Nurk","year":"2022","unstructured":"Nurk S, Koren S, Rhie A, Rautiainen M, Bzikadze AV, Mikheenko A, et al. The complete sequence of a human genome. Science. 2022;376(6588):44\u201353.","journal-title":"Science"},{"key":"7762_CR39","unstructured":"Widman AJ, Shah M, \u00d8gaard N, Khamnei CC, Frydendahl A, Deshpande A, et al. Machine learning guided signal enrichment for ultrasensitive plasma tumor burden monitoring. 2022."},{"key":"7762_CR40","doi-asserted-by":"crossref","unstructured":"Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. 2011. 2011;17(1):3.","DOI":"10.14806\/ej.17.1.200"},{"issue":"14","key":"7762_CR41","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009;25(14):1754\u201360.","journal-title":"Bioinformatics"},{"key":"7762_CR42","unstructured":"Broad Institute GT. MarkDuplicates (Picard): Broad Institute, GATK Team; [Available from: https:\/\/gatk.broadinstitute.org\/hc\/en-us\/articles\/360037052812-MarkDuplicates-Picard."},{"issue":"3","key":"7762_CR43","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403\u201310.","journal-title":"J Mol Biol"},{"key":"7762_CR44","unstructured":"Broad Institute GT. FilterSamReads: Broad Institute, GATK Team; [Available from: https:\/\/gatk.broadinstitute.org\/hc\/en-us\/articles\/360036882611-FilterSamReads-Picard."},{"key":"7762_CR45","doi-asserted-by":"crossref","unstructured":"Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, et al. Twelve years of SAMtools and BCFtools. Gigascience. 2021;10(2).","DOI":"10.1093\/gigascience\/giab008"},{"issue":"Web Server issu","key":"7762_CR46","doi-asserted-by":"publisher","first-page":"W628","DOI":"10.1093\/nar\/gkh466","volume":"32","author":"YL Orlov","year":"2004","unstructured":"Orlov YL, Potapov VN. Complexity: an internet resource for analysis of DNA sequence complexity. Nucleic Acids Res. 2004;32(Web Server issue):W628\u201333.","journal-title":"Nucleic Acids Res"},{"issue":"18","key":"7762_CR47","doi-asserted-by":"publisher","first-page":"2834","DOI":"10.1093\/bioinformatics\/btab203","volume":"37","author":"TL Bailey","year":"2021","unstructured":"Bailey TL. STREME: accurate and versatile sequence motif discovery. Bioinformatics. 2021;37(18):2834\u201340.","journal-title":"Bioinformatics"},{"issue":"6","key":"7762_CR48","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1101\/gr.229102","volume":"12","author":"WJ Kent","year":"2002","unstructured":"Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, et al. The human genome browser at UCSC. Genome Res. 2002;12(6):996\u20131006.","journal-title":"Genome Res"},{"key":"7762_CR49","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1471-2105-10-421","volume":"10","author":"C Camacho","year":"2009","unstructured":"Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, et al. BLAST+: architecture and applications. BMC Bioinf. 2009;10:421.","journal-title":"BMC Bioinf"},{"issue":"5","key":"7762_CR50","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1093\/clinchem\/hvab274","volume":"68","author":"TV Henriksen","year":"2022","unstructured":"Henriksen TV, Drue SO, Frydendahl A, Demuth C, Rasmussen MH, Reinert T, et al. Error Characterization and statistical modeling improves circulating tumor DNA detection by droplet digital PCR. Clin Chem. 2022;68(5):657\u201367.","journal-title":"Clin Chem"},{"key":"7762_CR51","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2021","unstructured":"R Core Team. R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing; 2021."}],"container-title":["Journal of Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12967-026-07762-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-026-07762-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12967-026-07762-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:30:12Z","timestamp":1773397812000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12967-026-07762-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,9]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["7762"],"URL":"https:\/\/doi.org\/10.1186\/s12967-026-07762-6","relation":{},"ISSN":["1479-5876"],"issn-type":[{"value":"1479-5876","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,9]]},"assertion":[{"value":"5 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All patients were treated and monitored according to National Guidelines. The study involving colorectal cancer (CRC) patients was approved by the Committees on Biomedical Research Ethics in the Central Region of Denmark (approval numbers 1\u201316\u201302\u2013453\u201314 and 1\u201310\u201372\u20133\u201318), while the study involving MIBC patients was approved by The National Committee on Health Research Ethics (#1706501). All participants provided written informed consent prior to inclusion. The study was conducted in accordance with the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"CLA reports sponsored research agreements with AccuraGen, Bio-Rad Laboratories, C2i Genomics, Natera, and Labcorp. LD has sponsored research agreements with C2i Genomics, Natera, AstraZeneca, Photocure, Veracyte, and Ferring and has an advisory\/consulting role at Ferring, MSD and UroGen. LD has received speaker honoraria from AstraZeneca, Pfizer and Roche and received travel support from MSD. The opinions, results, and conclusions reported in this article are those of the authors and are independent of any competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"364"}}