{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:37:38Z","timestamp":1787020658884,"version":"build-2736575974"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Prof. KH Ren\u00e9 Koczorek Stiftung"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Neuroinflammation"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recently, mineralocorticoid receptors (MR) were identified in peripheral nociceptive neurons, and their acute antagonism was responsible for immediate and short-lasting (non-genomic) antinociceptive effects. The same neurons were shown to produce the endogenous ligand aldosterone by the enzyme aldosterone synthase.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here, we investigate whether endogenous aldosterone contributes to inflammation-induced hyperalgesia via the distinct genomic regulation of specific pain signaling molecules in an animal model of Freund\u2019s complete adjuvant (FCA)-induced hindpaw inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Chronic intrathecal application of MR antagonist canrenoate-K (over 4\u2009days) attenuated nociceptive behavior in rats with FCA hindpaw inflammation suggesting a tonic activation of neuronal MR by endogenous aldosterone. Consistently, double immunofluorescence confocal microscopy showed abundant co-localization of MR with several pain signaling molecules such as TRPV1, CGRP, Nav1.8, and trkA whose enhanced expression of mRNA and proteins during inflammation was downregulated following i.t. canrenoate-K. More importantly, inhibition of endogenous aldosterone production in peripheral sensory neurons by continuous intrathecal delivery of a specific aldosterone synthase inhibitor prevented the inflammation-induced enhanced transcriptional expression of TRPV1, CGRP, Nav1.8, and trkA and subsequently attenuated nociceptive behavior. Evidence for such a genomic effect of endogenous aldosterone was supported by the demonstration of an enhanced nuclear translocation of MR in peripheral sensory dorsal root ganglia (DRG) neurons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Taken together, chronic inhibition of local production of aldosterone by its processing enzyme aldosterone synthase within peripheral sensory neurons may contribute to long-lasting downregulation of specific pain signaling molecules and may, thus, persistently reduce inflammation-induced hyperalgesia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12974-020-01864-8","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T05:04:12Z","timestamp":1591938252000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain"],"prefix":"10.1186","volume":"17","author":[{"given":"Mohammed","family":"Shaqura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa M.","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongjuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Treskatsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanze","family":"Buhrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Shakibaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaaban A.","family":"Mousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,12]]},"reference":[{"key":"1864_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.physbeh.2016.12.045","volume":"178","author":"ER de Kloet","year":"2017","unstructured":"de Kloet ER, Joels M. Brain mineralocorticoid receptor function in control of salt balance and stress-adaptation. Physiol Behav. 2017;178:13\u201320.","journal-title":"Physiol Behav"},{"issue":"Suppl 1","key":"1864_CR2","first-page":"65","volume":"125","author":"ER De Kloet","year":"1991","unstructured":"De Kloet ER, Sutanto W, Rots N, van Haarst A, van den Berg D, Oitzl M, van Eekelen A, Voorhuis D. Plasticity and function of brain corticosteroid receptors during aging. Acta Endocrinol (Copenh). 1991;125(Suppl 1):65\u201372.","journal-title":"Acta Endocrinol (Copenh)"},{"key":"1864_CR3","doi-asserted-by":"publisher","first-page":"T49","DOI":"10.1530\/JOE-16-0660","volume":"234","author":"M Joels","year":"2017","unstructured":"Joels M, de Kloet ER. 30 years of the mineralocorticoid receptor: the brain mineralocorticoid receptor: a saga in three episodes. J Endocrinol. 2017;234:T49\u2013t66.","journal-title":"J Endocrinol"},{"key":"1864_CR4","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1210\/js.2019-00158","volume":"3","author":"A Koning","year":"2019","unstructured":"Koning A, Buurstede JC, van Weert L, Meijer OC. Glucocorticoid and mineralocorticoid receptors in the brain: a transcriptional perspective. J Endocr Soc. 2019;3:1917\u201330.","journal-title":"J Endocr Soc"},{"key":"1864_CR5","doi-asserted-by":"publisher","first-page":"8640970","DOI":"10.1155\/2017\/8640970","volume":"2017","author":"KM Madalena","year":"2017","unstructured":"Madalena KM, Lerch JK. The effect of glucocorticoid and glucocorticoid receptor interactions on brain, spinal cord, and glial cell plasticity. Neural Plast. 2017;2017:8640970.","journal-title":"Neural Plast"},{"key":"1864_CR6","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.neuropharm.2016.03.032","volume":"107","author":"M Shaqura","year":"2016","unstructured":"Shaqura M, Li X, Al-Madol MA, Tafelski S, Beyer-Koczorek A, Mousa SA, Schafer M. Acute mechanical sensitization of peripheral nociceptors by aldosterone through non-genomic activation of membrane bound mineralocorticoid receptors in naive rats. Neuropharmacology. 2016;107:251\u201361.","journal-title":"Neuropharmacology"},{"key":"1864_CR7","doi-asserted-by":"crossref","unstructured":"Shaqura M, Li X, Al-Khrasani M, Shakibaei M, Tafelski S, Furst S, Beyer A, Kawata M, Schafer M, Mousa SA: Membrane-bound glucocorticoid receptors on distinct nociceptive neurons as potential targets for pain control through rapid non-genomic effects. Neuropharmacology 2016, 111:1-13.","DOI":"10.1016\/j.neuropharm.2016.08.019"},{"key":"1864_CR8","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1097\/ALN.0000000000002087","volume":"128","author":"X Li","year":"2018","unstructured":"Li X, Shaqura M, Mohamed D, Beyer A, Yamada S, Mousa SA, Schafer M. Pro- versus Antinociceptive Nongenomic Effects of neuronal mineralocorticoid versus glucocorticoid receptors during rat hind paw inflammation. Anesthesiology. 2018;128:796\u2013809.","journal-title":"Anesthesiology"},{"key":"1864_CR9","doi-asserted-by":"crossref","unstructured":"Mohamed DM, Shaqura M, Li X, Shakibaei M, Beyer A, Treskatsch S, Schafer M, Mousa SA: Aldosterone synthase in peripheral sensory neurons contributes to mechanical hypersensitivity during local inflammation in rats. Anesthesiology 2020.","DOI":"10.1097\/ALN.0000000000003127"},{"key":"1864_CR10","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/0304-3940(93)90758-D","volume":"149","author":"R Covenas","year":"1993","unstructured":"Covenas R, de Leon M, Cintra A, Bjelke B, Gustafsson JA, Fuxe K. Coexistence of c-Fos and glucocorticoid receptor immunoreactivities in the CRF immunoreactive neurons of the paraventricular hypothalamic nucleus of the rat after acute immobilization stress. Neurosci Lett. 1993;149:149\u201352.","journal-title":"Neurosci Lett"},{"key":"1864_CR11","doi-asserted-by":"publisher","first-page":"C423","DOI":"10.1152\/ajpcell.1994.266.2.C423","volume":"266","author":"N Farman","year":"1994","unstructured":"Farman N, Bonvalet JP, Seckl JR. Aldosterone selectively increases Na(+)-K(+)-ATPase alpha 3-subunit mRNA expression in rat hippocampus. Am J Physiol. 1994;266:C423\u20138.","journal-title":"Am J Physiol"},{"key":"1864_CR12","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.neuroscience.2016.04.050","volume":"329","author":"HW Wang","year":"2016","unstructured":"Wang HW, Huang BS, White RA, Chen A, Ahmad M, Leenen FH. Mineralocorticoid and angiotensin II type 1 receptors in the subfornical organ mediate angiotensin II - induced hypothalamic reactive oxygen species and hypertension. Neuroscience. 2016;329:112\u201321.","journal-title":"Neuroscience"},{"key":"1864_CR13","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.neuroscience.2018.08.031","volume":"390","author":"NJ Mills","year":"2018","unstructured":"Mills NJ, Sharma K, Haque M, Moore M, Teruyama R. Aldosterone mediated regulation of epithelial sodium channel (ENaC) subunits in the rat hypothalamus. Neuroscience. 2018;390:278\u201392.","journal-title":"Neuroscience"},{"key":"1864_CR14","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1097\/00000542-200108000-00036","volume":"95","author":"HL Rittner","year":"2001","unstructured":"Rittner HL, Brack A, Machelska H, Mousa SA, Bauer M, Schafer M, Stein C. Opioid peptide-expressing leukocytes: identification, recruitment, and simultaneously increasing inhibition of inflammatory pain. Anesthesiology. 2001;95:500\u20138.","journal-title":"Anesthesiology"},{"key":"1864_CR15","first-page":"686","volume":"185","author":"W Sadee","year":"1973","unstructured":"Sadee W, Dagcioglu M, Schroder R. Pharmacokinetics of spironolactone, canrenone and canrenoate-K in humans. J Pharmacol Exp Ther. 1973;185:686\u201395.","journal-title":"J Pharmacol Exp Ther"},{"key":"1864_CR16","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1172\/JCI106974","volume":"51","author":"GH Williams","year":"1972","unstructured":"Williams GH, Cain JP, Dluhy RG, Underwood RH. Studies of the control of plasma aldosterone concentration in normal man. I. Response to posture, acute and chronic volume depletion, and sodium loading. J Clin Invest. 1972;51:1731\u201342.","journal-title":"J Clin Invest"},{"key":"1864_CR17","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1002\/cpt1976202167","volume":"20","author":"L Ramsay","year":"1976","unstructured":"Ramsay L, Shelton J, Harrison I, Tidd M, Asbury M. Spironolactone and potassium canrenoate in normal man. Clin Pharmacol Ther. 1976;20:167\u201377.","journal-title":"Clin Pharmacol Ther"},{"key":"1864_CR18","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1124\/jpet.110.167148","volume":"334","author":"DF Rigel","year":"2010","unstructured":"Rigel DF, Fu F, Beil M, Hu CW, Liang G, Jeng AY. Pharmacodynamic and pharmacokinetic characterization of the aldosterone synthase inhibitor FAD286 in two rodent models of hyperaldosteronism: comparison with the 11beta-hydroxylase inhibitor metyrapone. J Pharmacol Exp Ther. 2010;334:232\u201343.","journal-title":"J Pharmacol Exp Ther"},{"key":"1864_CR19","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1016\/j.jpain.2013.01.776","volume":"14","author":"M Shaqura","year":"2013","unstructured":"Shaqura M, Khalefa BI, Shakibaei M, Winkler J, Al-Khrasani M, Furst S, Mousa SA, Schafer M. Reduced number, G protein coupling, and antinociceptive efficacy of spinal mu-opioid receptors in diabetic rats are reversed by nerve growth factor. J Pain. 2013;14:720\u201330.","journal-title":"J Pain"},{"key":"1864_CR20","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1097\/j.pain.0000000000000459","volume":"157","author":"SA Mousa","year":"2016","unstructured":"Mousa SA, Shaqura M, Winkler J, Khalefa BI, Al-Madol MA, Shakibaei M, Schulz S, Schafer M. Protein kinase C-mediated mu-opioid receptor phosphorylation and desensitization in rats, and its prevention during early diabetes. Pain. 2016;157:910\u201321.","journal-title":"Pain"},{"key":"1864_CR21","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402\u20138.","journal-title":"Methods"},{"key":"1864_CR22","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3:1101\u20138.","journal-title":"Nat Protoc"},{"key":"1864_CR23","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1124\/mol.113.088997","volume":"85","author":"V Spahn","year":"2014","unstructured":"Spahn V, Stein C, Zollner C. Modulation of transient receptor vanilloid 1 activity by transient receptor potential ankyrin 1. Mol Pharmacol. 2014;85:335\u201344.","journal-title":"Mol Pharmacol"},{"key":"1864_CR24","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1002\/cphy.c130044","volume":"4","author":"E Gomez-Sanchez","year":"2014","unstructured":"Gomez-Sanchez E, Gomez-Sanchez CE. The multifaceted mineralocorticoid receptor. Compr Physiol. 2014;4:965\u201394.","journal-title":"Compr Physiol"},{"key":"1864_CR25","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1097\/00041552-200403000-00007","volume":"13","author":"EP Gomez-Sanchez","year":"2004","unstructured":"Gomez-Sanchez EP. Brain mineralocorticoid receptors: orchestrators of hypertension and end-organ disease. Curr Opin Nephrol Hypertens. 2004;13:191\u20136.","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"1864_CR26","doi-asserted-by":"publisher","first-page":"4883","DOI":"10.1074\/jbc.273.9.4883","volume":"273","author":"JS Silvestre","year":"1998","unstructured":"Silvestre JS, Robert V, Heymes C, Aupetit-Faisant B, Mouas C, Moalic JM, Swynghedauw B, Delcayre C. Myocardial production of aldosterone and corticosterone in the rat. Physiological regulation. J Biol Chem. 1998;273:4883\u201391.","journal-title":"J Biol Chem"},{"key":"1864_CR27","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1097\/ALN.0b013e3182700383","volume":"117","author":"F Dong","year":"2012","unstructured":"Dong F, Xie W, Strong JA, Zhang JM. Mineralocorticoid receptor blocker eplerenone reduces pain behaviors in vivo and decreases excitability in small-diameter sensory neurons from local inflamed dorsal root ganglia in vitro. Anesthesiology. 2012;117:1102\u201312.","journal-title":"Anesthesiology"},{"key":"1864_CR28","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1111\/bcpt.12634","volume":"120","author":"V Jokinen","year":"2017","unstructured":"Jokinen V, Lilius T, Laitila J, Niemi M, Kambur O, Kalso E, Rauhala P. Do diuretics have antinociceptive actions: studies of spironolactone, eplerenone, furosemide and chlorothiazide, individually and with oxycodone and morphine. Basic Clin Pharmacol Toxicol. 2017;120:38\u201345.","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"1864_CR29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070505","volume":"8","author":"AV Derbenev","year":"2013","unstructured":"Derbenev AV, Smith BN. Dexamethasone rapidly increases GABA release in the dorsal motor nucleus of the vagus via retrograde messenger-mediated enhancement of TRPV1 activity. PLoS One. 2013;8:e70505.","journal-title":"PLoS One"},{"key":"1864_CR30","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1016\/0006-8993(94)91034-0","volume":"636","author":"M DeLeon","year":"1994","unstructured":"DeLeon M, Covenas R, Chadi G, Narvaez JA, Fuxe K, Cintra A. Subpopulations of primary sensory neurons show coexistence of neuropeptides and glucocorticoid receptors in the rat spinal and trigeminal ganglia. Brain Res. 1994;636:338\u201342.","journal-title":"Brain Res"},{"key":"1864_CR31","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/0006-8993(91)91347-4","volume":"564","author":"GD Smith","year":"1991","unstructured":"Smith GD, Seckl JR, Sheward WJ, Bennie JG, Carroll SM, Dick H, Harmar AJ. Effect of adrenalectomy and dexamethasone on neuropeptide content of dorsal root ganglia in the rat. Brain Res. 1991;564:27\u201330.","journal-title":"Brain Res"},{"key":"1864_CR32","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.neulet.2003.10.023","volume":"355","author":"RE Coggeshall","year":"2004","unstructured":"Coggeshall RE, Tate S, Carlton SM. Differential expression of tetrodotoxin-resistant sodium channels Nav1.8 and Nav1.9 in normal and inflamed rats. Neurosci Lett. 2004;355:45\u20138.","journal-title":"Neurosci Lett"},{"key":"1864_CR33","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1016\/j.jpain.2013.01.778","volume":"14","author":"L Liang","year":"2013","unstructured":"Liang L, Fan L, Tao B, Yaster M, Tao YX. Protein kinase B\/Akt is required for complete Freund's adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons. J Pain. 2013;14:638\u201347.","journal-title":"J Pain"},{"key":"1864_CR34","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1002\/cne.903490311","volume":"349","author":"RL Nahin","year":"1994","unstructured":"Nahin RL, Byers MR. Adjuvant-induced inflammation of rat paw is associated with altered calcitonin gene-related peptide immunoreactivity within cell bodies and peripheral endings of primary afferent neurons. J Comp Neurol. 1994;349:475\u201385.","journal-title":"J Comp Neurol"},{"key":"1864_CR35","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1002\/jnr.21844","volume":"87","author":"X Xu","year":"2009","unstructured":"Xu X, Wang P, Zou X, Li D, Fang L, Lin Q. Increases in transient receptor potential vanilloid-1 mRNA and protein in primary afferent neurons stimulated by protein kinase C and their possible role in neurogenic inflammation. J Neurosci Res. 2009;87:482\u201394.","journal-title":"J Neurosci Res"},{"key":"1864_CR36","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S0039-128X(99)00087-2","volume":"65","author":"FM Rogerson","year":"2000","unstructured":"Rogerson FM, Fuller PJ. Mineralocorticoid action. Steroids. 2000;65:61\u201373.","journal-title":"Steroids"},{"key":"1864_CR37","doi-asserted-by":"crossref","unstructured":"van Weert L, Buurstede JC, Sips HCM, Mol IM, Puri T, Damsteegt R, Roozendaal B, Sarabdjitsingh RA, Meijer OC. Mechanistic insights in neuroD potentiation of mineralocorticoid receptor signaling. Int J Mol Sci. 2019;20.","DOI":"10.3390\/ijms20071575"},{"key":"1864_CR38","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1210\/me.2008-0350","volume":"23","author":"G Hu","year":"2009","unstructured":"Hu G, Oboukhova EA, Kumar S, Sturek M, Obukhov AG. Canonical transient receptor potential channels expression is elevated in a porcine model of metabolic syndrome. Mol Endocrinol. 2009;23:689\u201399.","journal-title":"Mol Endocrinol"},{"key":"1864_CR39","doi-asserted-by":"publisher","first-page":"5121","DOI":"10.1210\/jcem.86.11.7925","volume":"86","author":"MJ Young","year":"2001","unstructured":"Young MJ, Clyne CD, Cole TJ, Funder JW. Cardiac steroidogenesis in the normal and failing heart. J Clin Endocrinol Metab. 2001;86:5121\u20136.","journal-title":"J Clin Endocrinol Metab"},{"key":"1864_CR40","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1007\/s00395-017-0643-0","volume":"112","author":"W Li","year":"2017","unstructured":"Li W, Chen X, Riley AM, Hiett SC, Temm CJ, Beli E, Long X, Chakraborty S, Alloosh M, White FA, et al. Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs. Basic Res Cardiol. 2017;112:54.","journal-title":"Basic Res Cardiol"},{"key":"1864_CR41","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.heares.2013.01.020","volume":"299","author":"SH Kil","year":"2013","unstructured":"Kil SH, Kalinec F. Expression and dexamethasone-induced nuclear translocation of glucocorticoid and mineralocorticoid receptors in guinea pig cochlear cells. Hear Res. 2013;299:63\u201378.","journal-title":"Hear Res"},{"key":"1864_CR42","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1186\/s12868-017-0352-5","volume":"18","author":"M Piechota","year":"2017","unstructured":"Piechota M, Korostynski M, Golda S, Ficek J, Jantas D, Barbara Z, Przewlocki R. Transcriptional signatures of steroid hormones in the striatal neurons and astrocytes. BMC Neurosci. 2017;18:37.","journal-title":"BMC Neurosci"}],"container-title":["Journal of Neuroinflammation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12974-020-01864-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12974-020-01864-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12974-020-01864-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T19:24:43Z","timestamp":1623439483000},"score":1,"resource":{"primary":{"URL":"https:\/\/jneuroinflammation.biomedcentral.com\/articles\/10.1186\/s12974-020-01864-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,12]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["1864"],"URL":"https:\/\/doi.org\/10.1186\/s12974-020-01864-8","relation":{},"ISSN":["1742-2094"],"issn-type":[{"value":"1742-2094","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,12]]},"assertion":[{"value":"24 March 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 June 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All animal experiments were approved by the local animal care committee (reference No. G0024\/14, Landesamt f\u00fcr Gesundheit und Soziales, LaGeSo Berlin) and according to the European Directive (2010\/63\/EU) introducing new animal welfare and care guideline.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no potential conflicts of interest with respect to the research, authorship, and\/or publication of this article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"183"}}