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However, whether <jats:italic>Tsc1<\/jats:italic> participates in regulating ROR\u03b3t-expressing cells remains unknown.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>We generated a mouse model in which <jats:italic>Tsc1<\/jats:italic> was conditionally deleted from <jats:italic>ROR\u03b3t-<\/jats:italic>expressing cells (<jats:italic>Tsc1<\/jats:italic><jats:sup><jats:italic>ROR\u03b3t<\/jats:italic><\/jats:sup>) to study the role of ROR\u03b3t-expressing cells with <jats:italic>Tsc1<\/jats:italic> deficiency in brain homeostasis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Type 3 innate lymphoid cells (ILC3s) in <jats:italic>Tsc1<\/jats:italic><jats:sup><jats:italic>ROR\u03b3t<\/jats:italic><\/jats:sup> mice displayed normal development and function, and the mice showed normal Th17 cell differentiation. However, <jats:italic>Tsc1<\/jats:italic><jats:sup><jats:italic>ROR\u03b3t<\/jats:italic><\/jats:sup> mice exhibited spontaneous tonic-clonic seizures and died between 4 and 6 weeks after birth. At the age of 4 weeks, mice in which <jats:italic>Tsc1<\/jats:italic> was specifically knocked out in <jats:italic>ROR\u03b3t-<\/jats:italic>expressing cells had cortical neuron defects and hippocampal structural abnormalities. Notably, over-activation of neurons and astrogliosis were observed in the cortex and hippocampus of <jats:italic>Tsc1<\/jats:italic><jats:sup><jats:italic>ROR\u03b3t<\/jats:italic><\/jats:sup> mice. Moreover, expression of the \u03b3-amino butyric acid (GABA) receptor in the brains of <jats:italic>Tsc1<\/jats:italic><jats:sup><jats:italic>ROR\u03b3t<\/jats:italic><\/jats:sup> mice was decreased, and GABA supplementation prolonged the lifespan of the mice to some extent. Further experiments revealed the presence of a group of rare <jats:italic>ROR\u03b3t-<\/jats:italic>expressing cells with high metabolic activity in the mouse brain.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our study verifies the critical role of previously unnoticed ROR\u03b3t-expressing cells in the brain and demonstrates that the Tsc1 signaling pathway in ROR\u03b3t-expressing cells is important for maintaining brain homeostasis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12974-021-02153-8","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T05:03:16Z","timestamp":1620277396000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Conditional knockout of Tsc1 in ROR\u03b3t-expressing cells induces brain damage and early death in mice"],"prefix":"10.1186","volume":"18","author":[{"given":"Yafei","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuju","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baige","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5488-8576","authenticated-orcid":false,"given":"Youcai","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,6]]},"reference":[{"issue":"1","key":"2153_CR1","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1038\/s41556-018-0205-1","volume":"21","author":"J Kim","year":"2019","unstructured":"Kim J, Guan KL. mTOR as a central hub of nutrient signalling and cell growth. 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