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B2021333 and B2021453"],"award-info":[{"award-number":["Nos. B2021333 and B2021453"]}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["Nos. 2020A1515111160, 2021A1515110986 and 2020A1515110735"],"award-info":[{"award-number":["Nos. 2020A1515111160, 2021A1515110986 and 2020A1515110735"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Reproductive Science Institute Innovation Team grants","award":["No.C-03"],"award-info":[{"award-number":["No.C-03"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Immun Ageing"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Morphological and functional alterations in aging reproductive organs result in decreased male fertility. The epididymis functions as the transition region for post-testicular sperm maturation. And we have previously demonstrated that the epididymal initial segment (IS), a region of the reproductive tract essential for sperm maturation and capacitation, undergoes considerable histological changes and chronic immune activation in mice during aging. However, the local aging-associated cellular and molecular changes in the aged epididymal IS are poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We conducted single-cell RNA sequencing analysis on the epididymal IS of young (3-month-old) and old (21-month-old) mice. In total, 10,027 cells from the epididymal IS tissues of young and old mice were obtained and annotated. The cell composition, including the expansion of a principal cell subtype and<jats:italic>Ms4a4b<\/jats:italic><jats:sup>Hi<\/jats:sup><jats:italic>Ms4a6b<\/jats:italic><jats:sup>Hi<\/jats:sup>T cells, changed with age. Aged principal cells displayed multiple functional gene expression changes associated with acrosome reaction and sperm maturation, suggesting an asynchronous process of sperm activation and maturation during epididymal transit. Meanwhile, aging-related altered pathways in immune cells, especially the \u201ccell chemotaxis\u201d in<jats:italic>Cx3cr1<\/jats:italic><jats:sup>Hi<\/jats:sup>epididymal dendritic cells (eDCs), were identified. The monocyte-specific expression of chemokine<jats:italic>Ccl8<\/jats:italic>increased with age in eDCs. And the aged epididymal IS showed increased inflammatory cell infiltration and cytokine secretion. Furthermore, cell\u2013cell communication analysis indicated that age increased inflammatory signaling in the epididymal IS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Contrary to the general pattern of lower immune responses in the male proximal genital tract, we revealed an inflammaging status in mouse epididymal initial segment. These findings will allow future studies to enable the delay of male reproductive aging via immune regulation.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12979-023-00345-9","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T10:02:28Z","timestamp":1683799348000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Single-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging"],"prefix":"10.1186","volume":"20","author":[{"given":"Jintao","family":"Zhuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangzhou","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiumin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangan","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibing","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,11]]},"reference":[{"issue":"4","key":"345_CR1","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1111\/j.1745-7262.2007.00280.x","volume":"9","author":"RJ Aitken","year":"2007","unstructured":"Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA. 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