{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T09:06:52Z","timestamp":1713604012075},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["AIDS Res Ther"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18\u00a0months of age, serological confirmation test is recommended to confirm HIV infection<jats:italic>.<\/jats:italic><\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Case presentation<\/jats:title>\n                <jats:p>Here we report the case of a patient tested positive with HIV-1, ELISA, Murex<jats:sup>\u00ae<\/jats:sup> Ag\u2044Ab Combination assay\u00a0(OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas<jats:sup>\u00ae<\/jats:sup> TaqMan<jats:sup>\u00ae<\/jats:sup> 96\/Cobas<jats:sup>\u00ae<\/jats:sup> Ampliprep<jats:sup>\u00ae<\/jats:sup> was 6.49log<jats:sub>10.<\/jats:sub> The virus could be sequenced in partial <jats:italic>gag<\/jats:italic> and <jats:italic>pol<\/jats:italic> genes and belonged to CRF02_AG clade.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12981-021-00342-2","type":"journal-article","created":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T10:11:12Z","timestamp":1618999872000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report"],"prefix":"10.1186","volume":"18","author":[{"given":"Edmond","family":"Tchiakpe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9 K.","family":"Keke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moussa","family":"Bachabi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flore Armande","family":"Gangbo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Halimatou","family":"Diop\u2011Ndiaye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coumba","family":"Toure\u2011Kane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akadiri","family":"Yessoufou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,21]]},"reference":[{"key":"342_CR1","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.ijid.2018.07.001","volume":"74","author":"L Shen","year":"2018","unstructured":"Shen L, Liu X, Wang T, Yang J, Wu X, Mao L. Contradictory results of serological confirmatory test and real-time PCR assays in diagnosis a patient of HIV-1. Int J\u00a0Infect Dis. 2018;74:38\u201340.","journal-title":"Int J Infect Dis"},{"issue":"5","key":"342_CR2","doi-asserted-by":"publisher","first-page":"380","DOI":"10.3855\/jidc.247","volume":"3","author":"K Bakhouch","year":"2009","unstructured":"Bakhouch K, Oulad-Lahcen A, Bensghir R, Blaghen M, Elfilali KM, Ezzikouri S, et al. The prevalence of resistance-associated mutations to protease and reverse transcriptase inhibitors in treatment-naive (HIV1)-infected individuals in Casablanca, Morocco. J Infect Dev Ctries. 2009;3:380\u201391.","journal-title":"J Infect Dev Ctries"},{"key":"342_CR3","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1126\/science.272.5265.1167","volume":"272","author":"JW Mellors","year":"1996","unstructured":"Mellors JW, Rinaldo Jr CR, Gupta P, White M, Todd A, Kingsley LA. Prognosis in HIV-1 infection predicted by the quantity of virus in plasma. Science. 1996;272:1167\u201370.","journal-title":"Science"},{"key":"342_CR4","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1002\/jcla.20238","volume":"22","author":"H Syed Iqbal","year":"2008","unstructured":"Syed Iqbal H, Balakrishnan P, \u00a0Murugavel KG, Suniti S. Performance Characteristics of a new Rapid Immunochromatographic Test for the detection of Antibodies to Human Immunodeficiency Virus (HIV) Types 1 and 2. J Clin Lab Anal. 2008;22:178\u201385.","journal-title":"J Clin Lab Anal."},{"key":"342_CR5","doi-asserted-by":"publisher","DOI":"10.2196\/10770","volume":"4","author":"RM Selik","year":"2018","unstructured":"Selik RM, Linley L. Viral loads within 6 weeks after diagnosis of HIV infection in early and later stages: observational study using national surveillance data. JMIR Public Health Surveill. 2018;4:e10770.","journal-title":"JMIR Public Health Surveill."},{"key":"342_CR6","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1128\/JCM.00685-10","volume":"49","author":"D Church","year":"2011","unstructured":"Church D, Gregson D, Lloyd T, Klein M, Beckthold B, Laupland K, Gill MJ. Comparison of the RealTime HIV-1, COBAS TaqMan 48 v1.0, Easy Q v1.2, and Versant v3.0 assays for determination of HIV-1 viral loads in a cohort of Canadian patients with diverse HIV subtype infections. J Clin Microbiol. 2011;49:118\u201324.","journal-title":"J Clin Microbiol."},{"key":"342_CR7","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1128\/JCM.02183-10","volume":"49","author":"VM Pierce","year":"2011","unstructured":"Pierce VM, Neide B, Hodinka RL. Evaluation of the Gen-Probe Aptima HIV-1 RNA qualitative assay as an alternative to Western blot analysis for confirmation of HIV infection. J Clin Microbiol. 2011;49:1642\u20135.","journal-title":"J Clin Microbiol."},{"key":"342_CR8","first-page":"509","volume":"36","author":"CDC","year":"1987","unstructured":"CDC. Perspectives in disease prevention and health promotion public health service guidelines for counseling and antibody testing to prevent HIV infection and AIDS. MMWR Morb Mortal Wkly Rep. 1987;36:509\u201315.","journal-title":"MMWR Morb Mortal Wkly Rep."},{"key":"342_CR9","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/365671a0","volume":"365","author":"BA Larder","year":"1993","unstructured":"Larder BA, Kohli A, Kellam P, Kemp SD, Kronick M, Henfrey RD. Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing. 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