{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:23:03Z","timestamp":1772504583413,"version":"3.50.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:00:00Z","timestamp":1423699200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1186\/s12985-015-0255-y","type":"journal-article","created":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T03:52:07Z","timestamp":1423626727000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae"],"prefix":"10.1186","volume":"12","author":[{"given":"Li","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liji","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianwen","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sisi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaoling","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,2,12]]},"reference":[{"issue":"4","key":"255_CR1","doi-asserted-by":"publisher","first-page":"e00495","DOI":"10.1128\/genomeA.00495-13","volume":"1","author":"L Teng","year":"2013","unstructured":"Teng L, Xie Z, Xie L, Liu J, Pang Y, Deng X, et al. Complete genome sequences of an avian orthoreovirus isolated from guangxi, china. Genome Announc. 2013;1(4):e00495\u201313.","journal-title":"Genome Announc"},{"key":"255_CR2","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1007\/s11262-013-1029-5","volume":"48","author":"L Teng","year":"2014","unstructured":"Teng L, Xie Z, Xie L, Liu J, Pang Y, Deng X, et al. Sequencing and phylogenetic analysis of an avian reovirus genome. Virus Genes. 2014;48:381\u20136.","journal-title":"Virus Genes"},{"key":"255_CR3","doi-asserted-by":"crossref","first-page":"614","DOI":"10.20506\/rst.19.2.1237","volume":"19","author":"RC Jones","year":"2000","unstructured":"Jones RC. Avian reovirus infections. Rev Sci Tech. 2000;19:614\u201325.","journal-title":"Rev Sci Tech"},{"key":"255_CR4","doi-asserted-by":"publisher","first-page":"638","DOI":"10.2307\/1593104","volume":"44","author":"L van der Heide","year":"2000","unstructured":"van der Heide L. The history of avian reovirus. Avian Dis. 2000;44:638\u201341.","journal-title":"Avian Dis"},{"key":"255_CR5","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1177\/030098589803500303","volume":"35","author":"SB Lockaby","year":"1998","unstructured":"Lockaby SB, Hoerr FJ, Lauerman LH, Kleven SH. Pathogenicity of Mycoplasma synoviae in broiler chickens. Vet Pathol. 1998;35:178\u201390.","journal-title":"Vet Pathol"},{"key":"255_CR6","first-page":"59","volume":"116","author":"KH Hinz","year":"2003","unstructured":"Hinz KH, Blome C, Ryll M. Virulence of Mycoplasma synoviae strains in experimentally infected broiler chickens. Berl Munch Tierarztl Wochenschr. 2003;116:59\u201366.","journal-title":"Berl Munch Tierarztl Wochenschr"},{"key":"255_CR7","doi-asserted-by":"crossref","unstructured":"Bradbury JM, Garuti A. Dual infection with Mycoplasma synoviae and a tenosynovitis-inducing reovirus in chickens. Avian Pathogy. 1978;7(3):407\u201319.","DOI":"10.1080\/03079457808418294"},{"key":"255_CR8","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1590\/S0100-736X2012000800001","volume":"32","author":"C Reck","year":"2012","unstructured":"Reck C, Menin \u00c1M, Pilati C, Milettia LC. Clinical and histologic lesions of mixed infection with Avian orthoreovirus and Mycoplasma synoviae in broilers. Pesqui Vet Bras. 2012;32:687\u201391.","journal-title":"Pesqui Vet Bras"},{"key":"255_CR9","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1080\/03079450400013170","volume":"33","author":"WJ Landman","year":"2004","unstructured":"Landman WJ, Feberwee A. Aerosol-induced Mycoplasma synoviae arthritis: the synergistic effect of infectious bronchitis virus infection. Avian Pathol. 2004;33:591\u20138.","journal-title":"Avian Pathol"},{"key":"255_CR10","doi-asserted-by":"publisher","first-page":"749","DOI":"10.2307\/1591778","volume":"36","author":"A Ortiz","year":"1992","unstructured":"Ortiz A, Kleven SH. Serological detection of Mycoplasma synoviae infection in turkeys. Avian Dis. 1992;36:749\u201352.","journal-title":"Avian Dis"},{"key":"255_CR11","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1637\/10425-101712-Reg.1","volume":"57","author":"C Reck","year":"2013","unstructured":"Reck C, Menin A, Canever MF, Milettia LC. Rapid detection of Mycoplasma synoviae and avian reovirus in clinical samples of poultry using multiplex PCR. Avian Dis. 2013;57:220\u20134.","journal-title":"Avian Dis"},{"issue":"Pt 8","key":"255_CR12","doi-asserted-by":"publisher","first-page":"2087","DOI":"10.1099\/13500872-145-8-2087","volume":"145","author":"AH Noormohammadi","year":"1999","unstructured":"Noormohammadi AH, Markham PF, Markham JF, Whithear KG, Browning GF. Mycoplasma synoviae surface protein MSPB as a recombinant antigen in an indirect ELISA. Microbiology. 1999;145(Pt 8):2087\u201394.","journal-title":"Microbiology"},{"key":"255_CR13","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1128\/JCM.43.2.948-950.2005","volume":"43","author":"B Ben Abdelmoumen Mardassi","year":"2005","unstructured":"Ben Abdelmoumen Mardassi B, Ben Mohamed R, Gueriri I, Boughattas S, Mlik B. Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes. J Clin Microbiol. 2005;43:948\u201358.","journal-title":"J Clin Microbiol"},{"key":"255_CR14","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1128\/JCM.01626-10","volume":"49","author":"S Troxler","year":"2011","unstructured":"Troxler S, Marek A, Prokofieva I, Bilic I, Hess M. TaqMan real-time reverse transcription-PCR assay for universal detection and quantification of avian hepatitis E virus from clinical samples in the presence of a heterologous internal control RNA. J Clin Microbiol. 2011;49:1339\u201346.","journal-title":"J Clin Microbiol"},{"key":"255_CR15","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1006\/meth.2001.1265","volume":"25","author":"CT Wittwer","year":"2001","unstructured":"Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS. Real-time multiplex PCR assays. Methods. 2001;25:430\u201342.","journal-title":"Methods"},{"key":"255_CR16","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.1093\/nar\/30.6.1292","volume":"30","author":"IM Mackay","year":"2002","unstructured":"Mackay IM, Arden KE, Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 2002;30:1292\u2013305.","journal-title":"Nucleic Acids Res"},{"key":"255_CR17","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.jviromet.2014.07.007","volume":"207","author":"Z Xie","year":"2014","unstructured":"Xie Z, Luo S, Xie L, Liu J, Pang Y, Deng X, et al. Simultaneous typing of nine avian respiratory pathogens using a novel GeXP analyzer-based multiplex PCR assay. J Virol Methods. 2014;207:188\u201395.","journal-title":"J Virol Methods"},{"key":"255_CR18","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1007\/s00705-008-0253-0","volume":"153","author":"Z Xie","year":"2008","unstructured":"Xie Z, Xie L, Pang Y, Lu Z, Xie Z, Sun J, et al. Development of a real-time multiplex PCR assay for detection of viral pathogens of penaeid shrimp. Arch Virol. 2008;153:2245\u201351.","journal-title":"Arch Virol"},{"key":"255_CR19","doi-asserted-by":"publisher","first-page":"7289","DOI":"10.1128\/AEM.69.12.7289-7297.2003","volume":"69","author":"J Vaitomaa","year":"2003","unstructured":"Vaitomaa J, Rantala A, Halinen K, Rouhiainen L, Tallberg P, Mokelke L, et al. Quantitative real-time PCR for determination of microcystin synthetase e copy numbers for microcystis and anabaena in lakes. Appl Environ Microbiol. 2003;69:7289\u201397.","journal-title":"Appl Environ Microbiol"},{"key":"255_CR20","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1016\/j.oooo.2011.12.007","volume":"113","author":"L Montebugnoli","year":"2012","unstructured":"Montebugnoli L, Venturi M, Gissi DB, Cervellati F. Clinical and histologic healing of lichenoid oral lesions following amalgam removal: a prospective study. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012;113:766\u201372.","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"255_CR21","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.eururo.2010.10.039","volume":"59","author":"A Heidenreich","year":"2011","unstructured":"Heidenreich A, Bellmunt J, Bolla M, Joniau S, Mason M, Matveev V, et al. EAU guidelines on prostate cancer. Part 1: screening, diagnosis, and treatment of clinically localised disease. Eur Urol. 2011;59:61\u201371.","journal-title":"Eur Urol"},{"key":"255_CR22","first-page":"501","volume":"35","author":"A Heidenreich","year":"2011","unstructured":"Heidenreich A, Bellmunt J, Bolla M, Joniau S, Mason M, Matveev V, et al. EAU guidelines on prostate cancer. Part I: screening, diagnosis, and treatment of clinically localised disease. Actas Urol Esp. 2011;35:501\u201314.","journal-title":"Actas Urol Esp"},{"issue":"5-6","key":"255_CR23","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.mcp.2014.07.001","volume":"28","author":"H Wu","year":"2014","unstructured":"Wu H, Rao P, Jiang Y, Opriessnig T, Yang Z. A sensitive multiplex real-time PCR panel for rapid diagnosis of viruses associated with porcine respiratory and reproductive disorders. Mol Cell Probes. 2014;28(5-6):264\u201370.","journal-title":"Mol Cell Probes"},{"key":"255_CR24","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1080\/03079451003604621","volume":"39","author":"AV Sprygin","year":"2010","unstructured":"Sprygin AV, Andreychuk DB, Kolotilov AN, Volkov MS, Runina IA, Mudrak NS, et al. Development of a duplex real-time TaqMan PCR assay with an internal control for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from commercial and backyard poultry. Avian Pathol. 2010;39:99\u2013109.","journal-title":"Avian Pathol"},{"key":"255_CR25","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1637\/9958-100911-ResNote.1","volume":"56","author":"N Jindal","year":"2012","unstructured":"Jindal N, Chander Y, Patnayak DP, Mor SK, Ziegler AF, Goyal SM. A multiplex RT-PCR for the detection of astrovirus, rotavirus, and reovirus in turkeys. Avian Dis. 2012;56:592\u20136.","journal-title":"Avian Dis"},{"key":"255_CR26","doi-asserted-by":"publisher","first-page":"654","DOI":"10.2307\/1592157","volume":"41","author":"Z Xie","year":"1997","unstructured":"Xie Z, Fadl AA, Girshick T, Khan MI. Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Dis. 1997;41:654\u201360.","journal-title":"Avian Dis"},{"key":"255_CR27","doi-asserted-by":"crossref","unstructured":"Kursa O, Wozniakowski G, Tomczyk G, Sawicka A, Minta Z. Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification. Arch Microbiol 2014. doi:10.1007\/s00203-014-1063-2","DOI":"10.1007\/s00203-014-1063-2"},{"key":"255_CR28","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1080\/03079457.2012.686104","volume":"41","author":"Z Xie","year":"2012","unstructured":"Xie Z, Peng Y, Luo S, Wang Y, Liu J, Pang Y, et al. Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus. Avian Pathol. 2012;41:311\u20136.","journal-title":"Avian Pathol"},{"key":"255_CR29","doi-asserted-by":"crossref","unstructured":"Wozniakowski G, Niczyporuk JS, Samorek-Salamonowicz E, Gawel A. The development and evaluation of cross-priming amplification (CPA) for the detection of avian reovirus (ARV). J Appl Microbiol 2014. doi:10.1111\/jam.12705.","DOI":"10.1111\/jam.12705"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-015-0255-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12985-015-0255-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-015-0255-y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-015-0255-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T08:22:43Z","timestamp":1630570963000},"score":1,"resource":{"primary":{"URL":"https:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/s12985-015-0255-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,12]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["255"],"URL":"https:\/\/doi.org\/10.1186\/s12985-015-0255-y","relation":{},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,12]]},"assertion":[{"value":"21 October 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 January 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 February 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"22"}}