{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T15:04:25Z","timestamp":1768921465568,"version":"3.49.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"National Natural Science Funds","award":["31201889; 31170146"],"award-info":[{"award-number":["31201889; 31170146"]}]},{"name":"Shandong Province Modern Agricultural Industry Technology System","award":["SDAIT-06-021-07"],"award-info":[{"award-number":["SDAIT-06-021-07"]}]},{"name":"Shandong Province Science and Technology Development Plan","award":["2014GNC111011"],"award-info":[{"award-number":["2014GNC111011"]}]},{"name":"Young Talents Training Program of Shandong Academy of Agricultural Sciences and the Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences","award":["2014QNM40"],"award-info":[{"award-number":["2014QNM40"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1186\/s12985-016-0597-0","type":"journal-article","created":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T13:39:55Z","timestamp":1479130795000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice"],"prefix":"10.1186","volume":"13","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaojian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianli","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuxuan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyuan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Cong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihui","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijun","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinbao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,14]]},"reference":[{"key":"597_CR1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/295064a0","volume":"295","author":"I Tischer","year":"1982","unstructured":"Tischer I, Gelderblom H, Vettermann W, Koch M. A very small porcine virus with circular single-stranded DNA. Nature. 1982;295:64\u20136.","journal-title":"Nature"},{"key":"597_CR2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0378-1135(99)00004-8","volume":"66","author":"GM Allan","year":"1999","unstructured":"Allan GM, McNeilly F, Meehan BM, Kennedy S, Mackie DP, Ellis JA, Clark EG, Espuna E, Saubi N, Riera P, B\u00f8tner A, Charreyre CE. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbio. 1999;66:115\u201323.","journal-title":"Vet Microbio"},{"key":"597_CR3","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/1348-0421.12244","volume":"59","author":"XQ Guo","year":"2015","unstructured":"Guo XQ, Wang LQ, Qiao H, Yang XW, Yang MF, Chen HY. Enhancement of the immunogenicity of a porcine circovirus type 2 DNA vaccine by a recombinant plasmid coexpressing capsid protein and porcine interleukin-6 in mice. Microbiol Immunol. 2015;59:174\u201380.","journal-title":"Microbiol Immunol"},{"key":"597_CR4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-8-1","volume":"8","author":"F Fu","year":"2011","unstructured":"Fu F, Li XS, Lang YK, Yang YJ, Tong GZ, Li GX, Zhou YJ, Li X. Co-expression of Ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice. Vriol J. 2011;8:1\u20139.","journal-title":"Vriol J"},{"key":"597_CR5","doi-asserted-by":"crossref","first-page":"e0147432","DOI":"10.1371\/journal.pone.0147432","volume":"11","author":"S Zhu","year":"2016","unstructured":"Zhu S, Zhang C, Wang J, Wei L, Quan R, Yang J, Yan X, Li Z, She R, Hu F, Liu J. Immunity Elicited by an Experimental Vaccine Based on Recombinant Flagellin-Porcine Circovirus Type 2 Cap Fusion Protein in Piglets. PLoS One. 2016;11:e0147432.","journal-title":"PLoS One"},{"key":"597_CR6","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1111\/1348-0421.12158","volume":"58","author":"S Sylla","year":"2014","unstructured":"Sylla S, Cong YL, Sun YX, Yang GL, Ding XM, Yang ZQ, Zhou YL, Yang MN, Wang CF, Ding Z. Protective immunity conferred by porcine circovirus 2 ORF2-based DNA vaccine in mice. Microbiol Immunol. 2014;58:398\u2013408.","journal-title":"Microbiol Immunol"},{"key":"597_CR7","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1016\/j.vaccine.2015.03.090","volume":"33","author":"H Zhang","year":"2015","unstructured":"Zhang H, Qian P, Peng B, Shi L, Chen HC, Li XM. A novel subunit vaccine co-expressing GM-CSF and PCV2b Cap protein enhances protective immunity against porcine circovirus type 2 in piglets. Vaccine. 2015;33:2449\u201356.","journal-title":"Vaccine"},{"key":"597_CR8","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1128\/IAI.71.2.857-863.2003","volume":"71","author":"S Gomis","year":"2003","unstructured":"Gomis S, Babiuk L, Godson DL, Allan B, Thrush T, Townsend H, Willson P, Waters E, Hecker R, Potter A. Protection of chickens against Escherichia coli infections by DNA containing CpG motifs. Infect Immun. 2003;71:857\u201363.","journal-title":"Infect Immun"},{"key":"597_CR9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jconrel.2004.02.022","volume":"97","author":"GK Mutwiri","year":"2004","unstructured":"Mutwiri GK, Nichani AK, Babiuk S, Babiuk LA. Strategies for enhancing the immunostimulatory effects of CpG oligodeoxynucleotides. J Control Release. 2004;97:1\u201317.","journal-title":"J Control Release"},{"key":"597_CR10","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1089\/vim.2014.0121","volume":"28","author":"J Li","year":"2015","unstructured":"Li J, Shi JL, Wu XY, Fu F, Yu J, Yuan XY, Peng Z, Cong XY, Xu SJ, Sun WB. Improvement of the immunogenicity of Porcine Circovirus Type 2 DNA vaccine by recombinant ORF2 gene and CpG motifs. Viral Immunol. 2015;28:290\u20136.","journal-title":"Viral Immunol"},{"key":"597_CR11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-7-1","volume":"7","author":"J Li","year":"2010","unstructured":"Li J, Yuan XY, Zhang CF, Miao LF, Wu JQ, Shi JL, Xu SJ, Cui SJ, Wang JB, Ai HB. A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse. Virol J. 2010;7:1\u20136.","journal-title":"Virol J"},{"key":"597_CR12","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1354\/vp.38-1-74","volume":"38","author":"M Kiupel","year":"2001","unstructured":"Kiupel M, Stevenson GW, Choi J, Latimer KS, Kanitz CL, Mittal SK. Viral replication and lesions in BALB\/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemic wasting disease. Vet Pathol. 2001;38:74\u201382.","journal-title":"Vet Pathol"},{"key":"597_CR13","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1136\/vr.101686","volume":"173","author":"J Li","year":"2013","unstructured":"Li J, Shi JL, Wu XY, Cong XY, Xu SJ, Yuan XY, Wu JQ, Sun WB, Du YJ, Peng Z, Wang JB, Huang BH. Differentiation of PCV1 and PCV2 by a multiplex real-time PCR assay. Vet Rec. 2013;173:346\u20137.","journal-title":"Vet Rec"},{"key":"597_CR14","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1016\/S0264-410X(02)00736-3","volume":"21","author":"L Fischer","year":"2003","unstructured":"Fischer L, Barzu S, Andreoni C, Buisson N, Brun A, Audonnet JC. DNA vaccination of neonate piglets in the face of maternal immunity induces humoral memory and protection against a virulent pseudorabies virus challenge. Vaccine. 2003;21:1732\u201341.","journal-title":"Vaccine"},{"key":"597_CR15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1574-695X.2001.tb01577.x","volume":"30","author":"RD Weeratna","year":"2001","unstructured":"Weeratna RD, Brazolot Millan CL, McCluskie MJ, Siegrist CA, Davis HL. Priming of immune responses to hepatitis B surface antigen in young mice immunized in the presence of maternally derived antibodies. FEMS Immunol Med Microbiol. 2001;30:241\u20137.","journal-title":"FEMS Immunol Med Microbiol"},{"key":"597_CR16","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/j.vaccine.2004.01.032","volume":"22","author":"S Kamstrup","year":"2004","unstructured":"Kamstrup S, Barfoed AM, Frimann TH, Ladekj\u00e6r-Mikkelsen AS, B\u00f8tner A. Immunisation against PCV2 structural protein by DNA vaccination of mice. Vaccine. 2004;22:1358\u201361.","journal-title":"Vaccine"},{"key":"597_CR17","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1586\/14760584.7.2.175","volume":"7","author":"S Lu","year":"2008","unstructured":"Lu S, Wang S, Grimes-Serrano JM. Current progress of DNA vaccine studies in humans. Expert Rev Vaccines. 2008;7:175\u201391.","journal-title":"Expert Rev Vaccines"},{"key":"597_CR18","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1016\/j.vaccine.2007.01.120","volume":"25","author":"JR Greenland","year":"2007","unstructured":"Greenland JR, Letvin NL. Chemical adjuvants for plasmid DNA vaccines. Vaccine. 2007;25:3731\u201341.","journal-title":"Vaccine"},{"key":"597_CR19","doi-asserted-by":"crossref","first-page":"5736","DOI":"10.1016\/j.vaccine.2013.09.035","volume":"31","author":"B Dong","year":"2013","unstructured":"Dong B, Feng J, Lin H, Li LXSD, Tu D, Zhu W, Yang Q, Ren X. Immune responses of mice immunized by DNA plasmids encoding PCV2 ORF 2 gene, porcine IL-15 or the both. Vaccine. 2013;31:5736\u201344.","journal-title":"Vaccine"},{"key":"597_CR20","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.virusres.2012.10.029","volume":"171","author":"F Fu","year":"2013","unstructured":"Fu F, Lang Y, Li X, Chai Z, Tong G, Liu S, Tian H, Li H, Wang W, Chen X, Li X. Evaluation of the enhancing ability of three adjuvants for DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice. Virus Res. 2013;171:247\u201351.","journal-title":"Virus Res"},{"key":"597_CR21","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1089\/vim.2014.0036","volume":"27","author":"GL Chen","year":"2014","unstructured":"Chen GL, Fu PF, Wang LQ, Li XS, Chen HY. Immune responses of piglets immunized by a recombinant plasmid containing porcine circovirus type 2 and porcine interleukin-18 genes. Viral Immunol. 2014;27:521\u20138.","journal-title":"Viral Immunol"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-016-0597-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T10:20:00Z","timestamp":1657707600000},"score":1,"resource":{"primary":{"URL":"http:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/s12985-016-0597-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,14]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["597"],"URL":"https:\/\/doi.org\/10.1186\/s12985-016-0597-0","relation":{},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,14]]},"article-number":"185"}}