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A lack of basic sanitation and sewage collection results in the presence of this virus in food, especially in oysters, since they are bioaccumulators and are consumed in their natural form, thus posing a risk to human health.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>This study investigated the frequency of HPyV2 in samples of oysters marketed in northeastern Par\u00e1 State, Brazil, and optimized a real-time PCR (qPCR) protocol for the detection of an endogenous oyster control. A total of 217 oysters in 22 pools from five municipalities in the state of Par\u00e1 were analyzed. Samples underwent dissection and total maceration of oyster tissue using a viral concentration technique, followed by DNA extraction with phenol-chloroform and amplification of the VP1 region for molecular detection via qPCR.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>HPyV2 was detected in 18.2% (4\/22) of the pooled samples, with frequencies of 25, 20, 20 and 16% in the municipalities of Salin\u00f3polis, Augusto Corr\u00eaa, S\u00e3o Caetano de Odivelas and Curu\u00e7\u00e1, respectively. Notably, the sample pool from the municipality of Bragan\u00e7a did not have detectable HPyV2 and this was the only sampled location with a water treatment station. In this study, <jats:italic>Crassostrea<\/jats:italic> genus-specific primers (<jats:italic>AFL52<\/jats:italic> ribosomal RNA gene) of oyster were developed for use as an endogenous control in the qPCR analysis, which will be useful for future studies.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The detection of HPyV2 in oyster samples commercialized in the state of Par\u00e1 shows the circulation of this virus in the studied municipalities. Thus, it is necessary to implement measures for improving sewage collection and basic sanitation to avoid contamination of water and food with HPyV2.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12985-020-01360-8","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T05:02:44Z","timestamp":1593147764000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Human polyomavirus 2 (HPyV2) in oyster samples in northern Brazil"],"prefix":"10.1186","volume":"17","author":[{"given":"Isabella Nogueira","family":"Abreu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline Monteiro","family":"Cortinhas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike Barbosa","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alice Freitas","family":"Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a Nazar\u00e9 Monteiro Rangel","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izaura Maria Vieira","family":"Cayres-Vallinoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1135-6507","authenticated-orcid":false,"given":"Antonio Carlos Ros\u00e1rio","family":"Vallinoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,26]]},"reference":[{"issue":"7712","key":"1360_CR1","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1016\/S0140-6736(71)91777-6","volume":"1","author":"BL Padgett","year":"1971","unstructured":"Padgett BL, Zurhein GM, Walker DL, Eckroade RJ. 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