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The <jats:italic>env<\/jats:italic> gene inserted into the fowlpox vector was verified for correct transgene expression by Western blotting and by immunofluorescence in different cell lines. The production of virus-like particles as a result of <jats:italic>env<\/jats:italic> gene expression was also demonstrated by electron microscopy. BALB\/c mice were immunosuppressed with dexamethasone and immunized following a prime\u2013boost strategy in a heterologous protocol where pVAX<jats:italic>zenv<\/jats:italic> was followed by FP<jats:italic>zenv<\/jats:italic>, to evaluate the immunogenicity of the Env protein. The mice underwent a challenge with an epidemic ZIKV after the last boost.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>These data show that the ZIKV Env protein was correctly expressed in both normal human lung fibroblasts (MRC-5 cells) and green monkey kidney (Vero) cells infected with FP<jats:italic>zenv<\/jats:italic>, and that the transgene expression lasted for more than 2\u00a0weeks. After mucosal administration of FP<jats:italic>zenv<\/jats:italic>, the immunized mice showed specific and significantly higher humoral responses compared to the control mice. However, virus neutralizing antibodies were not detected using plaque reduction assays.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Although BALB\/c mice appear to be an adequate model for ZIKV infection, as it mimics the natural mild infection in human beings, inadequate immune suppression seemed to occur by dexamethasone and different immune suppression strategies should be applied before challenge to reveal any protection of the mice.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12985-021-01519-x","type":"journal-article","created":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T14:03:26Z","timestamp":1614866606000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Construction of a recombinant avipoxvirus expressing the env gene of Zika virus as a novel putative preventive vaccine"],"prefix":"10.1186","volume":"18","author":[{"given":"Carlo","family":"Zanotto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Paolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5683-6216","authenticated-orcid":false,"given":"Antonia","family":"Radaelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"De Giuli Morghen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,4]]},"reference":[{"key":"1519_CR1","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/S1473-3099(18)30063-X","volume":"18","author":"GA Poland","year":"2018","unstructured":"Poland GA, Kennedy RB, Ovsyannikova I, Palacios R, Ho PL, Kalil J. 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