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They comprise several arthropod-borne viruses, including dengue virus, yellow fever virus, Japanese encephalitis virus, and West Nile virus.<jats:italic>Culex<\/jats:italic>flavivirus (CxFV) is a member of the insect-specific flavivirus (ISF) group belonging to the genus<jats:italic>Flavivirus<\/jats:italic>, which is widely distributed in a variety of mosquito populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Viral nucleic acid was extracted from adult mosquito pools and subjected to reverse transcriptase nested polymerase chain reaction (PCR) using target-specific primers for detecting CxFV nonstructural protein 5 (NS5). The PCR-positive samples were then sequenced, and a phylogenetic tree was constructed, including reference sequences obtained from GenBank.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>21 pools, belonging to<jats:italic>Culex pipiens pallens<\/jats:italic>(<jats:italic>Cx. p. pallens)<\/jats:italic>were found to be positive for the CxFV RNA sequence, with a minimum infection rate of 14.5\/1000 mosquitoes. The phylogenetic analysis of the NS5 protein sequences indicated that the detected sequences were closely related to strains identified in China, with 95\u201398% sequence similarities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings highlight the presence of CxFV in<jats:italic>Cx. p. pallens<\/jats:italic>mosquito species in Jeju province, Republic of Korea. This is the first study reporting the prevalence of CxFV in<jats:italic>Culex Pipiens (Cx. pipiens)<\/jats:italic>host in the Jeju province, which can create possible interaction with other flaviviruses causing human and animal diseases. 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