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One of the most notable features of ZIKV pathogenesis was the ability of the virus to pass the placental barrier to infect the growing foetus. This pathogenic trait had not been observed previously for medically important flaviviruses, including dengue and yellow fever viruses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study we evaluated the replication kinetics of ZIKV and the related encephalitic flavivirus West Nile strain Kunjin virus (WNV<jats:sub>KUN<\/jats:sub>) in early-term placental cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We have observed that WNV<jats:sub>KUN<\/jats:sub>in fact replicates with a greater rate and to higher titres that ZIKV in these cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results would indicate the potential for all flaviviruses to replicate in placental tissue but it is the ability to cross the placenta itself that is the restrictive factor in the clinical progression and presentation of congenital Zika syndrome.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12985-021-01720-y","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T10:02:55Z","timestamp":1639476175000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Flavivirus replication kinetics in early-term placental cell lines with different differentiation pathways"],"prefix":"10.1186","volume":"18","author":[{"given":"Julio","family":"Carrera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice M.","family":"Trenerry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron P.","family":"Simmons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-8350","authenticated-orcid":false,"given":"Jason M.","family":"Mackenzie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,12,14]]},"reference":[{"key":"1720_CR1","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1001\/jamaophthalmol.2016.0267","volume":"134","author":"FB de Paula","year":"2016","unstructured":"de Paula FB, de Oliveira Dias JR, Prazeres J, Sacramento GA, Ko AI, Maia M, Belfort R Jr. Ocular findings in infants with microcephaly associated with presumed Zika virus congenital infection in Salvador, Brazil. 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