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This study aimed to determine the minimum infection rates (MIRs) of CHIKV, DENV serotypes, and ZIKV in <jats:italic>Ae. albopictus<\/jats:italic> collected from selected two-site categories by altitude (highland [H] and lowland [L] sites) in Cebu city, Philippines during the wet (WS) and dry seasons (DS) of 2021\u20132022, and to explore the relationships between these arboviral MIRs and the local weather.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The viral RNA extracts in pooled and reared adult <jats:italic>Ae. albopictus<\/jats:italic> collected during the DS and WS from two-site categories were subjected to RT-PCR to amplify and detect gene loci specific for CHIKV, DENV-1 to DENV-4, and ZIKV and analyzed with the weather data.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The range of CHIKV MIRs was higher in the WS (13.61\u2013107.38 infected individuals per 1,000 mosquitoes) than in the DS (13.22\u201344.12), but was similar between the two-site categories. Rainfall (RF) influenced the CHIKV MIR. The MIR ranges of both DENV-2 (WS: H\u2009=\u20090, L\u2009=\u20090; DS: H\u2009=\u20090\u20135.92; L\u2009=\u20090\u20132.6) and DENV-4 (WS: H\u2009=\u20090, L\u2009=\u20090\u20132.90; DS: H\u2009=\u20092.96\u20136.13, L\u2009=\u20090\u201315.63) differed by season but not between the two-site categories. Relative humidity (RH), RF, and temperature did not influence DENVs\u2019 MIRs. The MIR range of ZIKV was similar in both seasons (WS: 11.36\u201340.27; DS: 0\u201346.15) and two-site categories (H\u2009=\u20090\u201390.91, L\u2009=\u20090\u201355.56). RH and temperature influenced ZIKV MIR.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>RF influenced CHIKV MIR in <jats:italic>Ae. albopictus<\/jats:italic>, whereas RH and temperature influenced that of ZIKV. Season influenced the MIRs of CHIKV and DENVs but not in ZIKV. <jats:italic>Ae. albopictus<\/jats:italic> were co-infected with CHIKV, DENVs, and ZIKV in both highland and lowland sites in Cebu city. Recommendations include all-year-round implementation of the Philippine Department of Health\u2019s <jats:italic>\u00a04S<\/jats:italic> enhanced strategy and installation of water pipelines in rural highlands for vector and disease control. Our findings are relevant to protect public health in the tropics in this climate change.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12985-024-02310-4","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T16:02:39Z","timestamp":1708012959000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detecting the impacts of humidity, rainfall, temperature, and season on chikungunya, dengue and Zika viruses in Aedes albopictus mosquitoes from selected sites in Cebu city, Philippines"],"prefix":"10.1186","volume":"21","author":[{"given":"Frances","family":"Edillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhoniel Ryan","family":"Ymbong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthoddiemn Olin","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen Mathilde","family":"Cabahug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristilynn","family":"Saavedra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"2310_CR1","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1089\/vbz.2006.0562","volume":"7","author":"MQ Benedict","year":"2007","unstructured":"Benedict MQ, Levine RS, Hawley WA, Lounibos LP. 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