{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:00:10Z","timestamp":1777489210999,"version":"3.51.4"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hepatitis E virus (HEV) is a positive-sense, single-stranded RNA virus and causes primarily acute self-limiting infections. The ORF1 of the HEV genome encodes a polyprotein around 190\u00a0kDa, which contains several putative domains, including helicase and RNA-dependent RNA polymerase. The HEV-encoded helicase is a member of the superfamily 1 helicase family and possesses multiple enzymatic functions, such as RNA 5\u2032-triphosphatase, RNA unwinding, and NTPase, which are thought to contribute to viral RNA synthesis. However, the helicase interaction with cellular proteins remains less known. Oxysterol binding protein (OSBP) is a lipid regulator that shuffles between the Golgi apparatus and the endoplasmic reticulum for cholesterol and phosphatidylinositol-4-phosphate exchange and controls the efflux of cholesterol from cells. In this study, the\u00a0RNAi-mediated silencing of OSBP significantly reduced HEV replication. Further studies indicate that the HEV helicase interacted with OSBP, shown by co-immunoprecipitation and co-localization in co-transfected cells. The presence of helicase blocked OSBP preferential translocation to the Golgi apparatus. These results demonstrate that OSBP contributes to HEV replication and enrich our understanding of the HEV-cell interactions.<\/jats:p>","DOI":"10.1186\/s12985-024-02438-3","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T16:18:27Z","timestamp":1721665107000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oxysterol binding protein (OSBP) contributes to hepatitis E virus replication"],"prefix":"10.1186","volume":"21","author":[{"given":"Shaoli","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peixi","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shane","family":"Tsao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail","family":"Aderinwale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhargava Teja","family":"Sallapalli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"key":"2438_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S1462399499001271","volume":"1999","author":"S Jameel","year":"1999","unstructured":"Jameel S. Molecular biology and pathogenesis of hepatitis E virus. Expert Rev Mol Med. 1999;1999:1\u201316.","journal-title":"Expert Rev Mol Med"},{"key":"2438_CR2","first-page":"3051","volume-title":"Fields virology","author":"RH Purcell","year":"2001","unstructured":"Purcell RH, Emerson SU. Hepatitis E virus. In: Knipe DM, Howley PM, editors. Fields virology, vol. 2, 4th ed. Philadelphia: Lippincott Williams Wilkins; 2001. pp. 3051\u201361."},{"key":"2438_CR3","doi-asserted-by":"publisher","first-page":"e1929","DOI":"10.1002\/rmv.1929","volume":"27","author":"MT Perez-Gracia","year":"2017","unstructured":"Perez-Gracia MT, Suay-Garcia B, Mateos-Lindemann ML. Hepatitis E and pregnancy: current state. Rev Med Virol. 2017;27:e1929.","journal-title":"Rev Med Virol"},{"key":"2438_CR4","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.jcv.2015.10.011","volume":"73","author":"T Lachish","year":"2015","unstructured":"Lachish T, Erez O, Daudi N, Shouval D, Schwartz E. Acute hepatitis E virus in pregnant women in Israel and in other industrialized countries. J Clin Virol. 2015;73:20\u20134.","journal-title":"J Clin Virol"},{"key":"2438_CR5","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1128\/CMR.00057-13","volume":"27","author":"N Kamar","year":"2014","unstructured":"Kamar N, Dalton HR, Abravanel F, Izopet J. Hepatitis E virus infection. Clin Microbiol Rev. 2014;27:116\u201338.","journal-title":"Clin Microbiol Rev"},{"key":"2438_CR6","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.3389\/fmicb.2016.01419","volume":"7","author":"Y Nan","year":"2016","unstructured":"Nan Y, Zhang YJ. Molecular biology and infection of hepatitis E virus. Front Microbiol. 2016;7:1419.","journal-title":"Front Microbiol"},{"key":"2438_CR7","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1002\/hep.26987","volume":"60","author":"C Honer zu Siederdissen","year":"2014","unstructured":"Honer zu Siederdissen C, Pischke S, Schlue J, Deterding K, Hellms T, Schuler-Luttmann S, Schwarz A, Manns MP, Cornberg M, Wedemeyer H. Chronic hepatitis E virus infection beyond transplantation or human immunodeficiency virus infection. Hepatology. 2014;60:1112\u20133.","journal-title":"Hepatology"},{"key":"2438_CR8","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.jhep.2016.02.045","volume":"65","author":"Y Debing","year":"2016","unstructured":"Debing Y, Moradpour D, Neyts J, Gouttenoire J. Update on hepatitis E virology: implications for clinical practice. J Hepatol. 2016;65:200\u201312.","journal-title":"J Hepatol"},{"key":"2438_CR9","doi-asserted-by":"publisher","first-page":"2223","DOI":"10.1099\/vir.0.068429-0","volume":"95","author":"DB Smith","year":"2014","unstructured":"Smith DB, Simmonds P, Members Of The International Committee On The Taxonomy Of Viruses Study G, Jameel S, Emerson SU, Harrison TJ, Meng XJ, Okamoto H, Van der Poel WHM, Purdy MA. Consensus proposals for classification of the family Hepeviridae. J Gen Virol. 2014;95:2223\u201332.","journal-title":"J Gen Virol"},{"key":"2438_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040869","author":"S Sridhar","year":"2017","unstructured":"Sridhar S, Teng JLL, Chiu TH, Lau SKP, Woo PCY. Hepatitis E virus genotypes and evolution: emergence of camel hepatitis E variants. Int J Mol Sci. 2017. https:\/\/doi.org\/10.3390\/ijms18040869.","journal-title":"Int J Mol Sci"},{"key":"2438_CR11","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1111\/j.1365-2893.2009.01257.x","volume":"17","author":"XJ Meng","year":"2010","unstructured":"Meng XJ. Recent advances in hepatitis E virus. J Viral Hepat. 2010;17:153\u201361.","journal-title":"J Viral Hepat"},{"key":"2438_CR12","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.virusres.2011.02.011","volume":"161","author":"I Ahmad","year":"2011","unstructured":"Ahmad I, Holla RP, Jameel S. Molecular virology of hepatitis E virus. Virus Res. 2011;161:47\u201358.","journal-title":"Virus Res"},{"key":"2438_CR13","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/nrgastro.2017.150","volume":"15","author":"I Nimgaonkar","year":"2018","unstructured":"Nimgaonkar I, Ding Q, Schwartz RE, Ploss A. Hepatitis E virus: advances and challenges. Nat Rev Gastroenterol Hepatol. 2018;15:96\u2013110.","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"355\u2013357","key":"2438_CR14","first-page":"e353","volume":"150","author":"GH Lee","year":"2016","unstructured":"Lee GH, Tan BH, Teo EC, Lim SG, Dan YY, Wee A, Aw PP, Zhu Y, Hibberd ML, Tan CK, et al. Chronic infection with camelid hepatitis E virus in a liver transplant recipient who regularly consumes camel meat and milk. Gastroenterology. 2016;150(355\u2013357):e353.","journal-title":"Gastroenterology"},{"key":"2438_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/v13020267","author":"S Lin","year":"2021","unstructured":"Lin S, Zhang YJ. Advances in hepatitis E virus biology and pathogenesis. Viruses. 2021. https:\/\/doi.org\/10.3390\/v13020267.","journal-title":"Viruses"},{"key":"2438_CR16","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001778","author":"MA Purdy","year":"2022","unstructured":"Purdy MA, Drexler JF, Meng XJ, Norder H, Okamoto H, Van der Poel WHM, Reuter G, de Souza WM, Ulrich RG, Smith DB. ICTV virus taxonomy profile: Hepeviridae 2022. J Gen Virol. 2022. https:\/\/doi.org\/10.1099\/jgv.0.001778.","journal-title":"J Gen Virol"},{"key":"2438_CR17","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s13062-015-0050-0","volume":"10","author":"T Ahola","year":"2015","unstructured":"Ahola T, Karlin DG. Sequence analysis reveals a conserved extension in the capping enzyme of the alphavirus supergroup, and a homologous domain in nodaviruses. Biol Direct. 2015;10:16.","journal-title":"Biol Direct"},{"key":"2438_CR18","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1038\/nrm3154","volume":"12","author":"P Linder","year":"2011","unstructured":"Linder P, Jankowsky E. From unwinding to clamping\u2014the DEAD box RNA helicase family. Nat Rev Mol Cell Biol. 2011;12:505\u201316.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2438_CR19","doi-asserted-by":"publisher","first-page":"3595","DOI":"10.1128\/JVI.02130-09","volume":"84","author":"YA Karpe","year":"2010","unstructured":"Karpe YA, Lole KS. NTPase and 5\u2019 to 3\u2019 RNA duplex-unwinding activities of the hepatitis E virus helicase domain. J Virol. 2010;84:3595\u2013602.","journal-title":"J Virol"},{"key":"2438_CR20","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.virusres.2013.11.022","volume":"179","author":"V Mhaindarkar","year":"2014","unstructured":"Mhaindarkar V, Sharma K, Lole KS. Mutagenesis of hepatitis E virus helicase motifs: effects on enzyme activity. Virus Res. 2014;179:26\u201333.","journal-title":"Virus Res"},{"key":"2438_CR21","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1111\/j.1439-0396.2007.00752.x","volume":"92","author":"P Nguyen","year":"2008","unstructured":"Nguyen P, Leray V, Diez M, Serisier S, Le Bloc\u2019h J, Siliart B, Dumon H. Liver lipid metabolism. J Anim Physiol Anim Nutr (Berlin). 2008;92:272\u201383.","journal-title":"J Anim Physiol Anim Nutr (Berlin)"},{"key":"2438_CR22","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1038\/s41580-019-0190-7","volume":"21","author":"J Luo","year":"2020","unstructured":"Luo J, Yang H, Song BL. Mechanisms and regulation of cholesterol homeostasis. Nat Rev Mol Cell Biol. 2020;21:225\u201345.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2438_CR23","volume-title":"Endotext","author":"KR Feingold","year":"2000","unstructured":"Feingold KR, Grunfeld C, et al. Introduction to lipids and lipoproteins. In: Feingold KR, Anawalt B, Boyce A, Chrousos G, de Herder WW, Dungan K, Grossman A, Hershman JM, Hofland HJ, Kaltsas G, et al., editors. Endotext. South Dartmouth: MDText.com Inc; 2000."},{"key":"2438_CR24","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1111\/tra.12260","volume":"16","author":"P Holla","year":"2015","unstructured":"Holla P, Ahmad I, Ahmed Z, Jameel S. Hepatitis E virus enters liver cells through a dynamin-2, clathrin and membrane cholesterol-dependent pathway. Traffic. 2015;16:398\u2013416.","journal-title":"Traffic"},{"key":"2438_CR25","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/1476-511X-9-5","volume":"9","author":"L Luo","year":"2010","unstructured":"Luo L, Pu X, Wang Y, Xu N. Impaired plasma lipid profiles in acute hepatitis. Lipids Health Dis. 2010;9:5.","journal-title":"Lipids Health Dis"},{"key":"2438_CR26","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.jcmgh.2021.02.002","volume":"12","author":"M Glitscher","year":"2021","unstructured":"Glitscher M, Martin DH, Woytinek K, Schmidt B, Tabari D, Scholl C, Stingl JC, Seelow E, Choi M, Hildt E. Targeting cholesterol metabolism as efficient antiviral strategy against the hepatitis E virus. Cell Mol Gastroenterol Hepatol. 2021;12:159\u201380.","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"2438_CR27","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1139\/o03-088","volume":"82","author":"VM Olkkonen","year":"2004","unstructured":"Olkkonen VM, Levine TP. Oxysterol binding proteins: In more than one place at one time? Biochem Cell Biol. 2004;82:87\u201398.","journal-title":"Biochem Cell Biol"},{"key":"2438_CR28","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1016\/j.cell.2013.09.056","volume":"155","author":"B Mesmin","year":"2013","unstructured":"Mesmin B, Bigay J, von Moser Filseck J, Lacas-Gervais S, Drin G, Antonny B. A four-step cycle driven by PI(4)P hydrolysis directs sterol\/PI(4)P exchange by the ER-Golgi tether OSBP. Cell. 2013;155:830\u201343.","journal-title":"Cell"},{"key":"2438_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.09.056","author":"B Mesmin","year":"2013","unstructured":"Mesmin B, Bigay J, Von Filseck JM, Lacas-Gervais S, Drin G, Antonny B. A four-step cycle driven by PI(4)P hydrolysis directs sterol\/PI(4)P exchange by the ER-Golgi tether OSBP. Cell. 2013. https:\/\/doi.org\/10.1016\/j.cell.2013.09.056.","journal-title":"Cell"},{"key":"2438_CR30","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.biochi.2018.12.013","volume":"158","author":"A Koponen","year":"2019","unstructured":"Koponen A, Arora A, Takahashi K, Kentala H, Kivela AM, Jaaskelainen E, Peranen J, Somerharju P, Ikonen E, Viitala T, Olkkonen VM. ORP2 interacts with phosphoinositides and controls the subcellular distribution of cholesterol. Biochimie. 2019;158:90\u2013101.","journal-title":"Biochimie"},{"key":"2438_CR31","doi-asserted-by":"publisher","first-page":"800","DOI":"10.15252\/embr.201541108","volume":"17","author":"R Galmes","year":"2016","unstructured":"Galmes R, Houcine A, van Vliet AR, Agostinis P, Jackson CL, Giordano F. ORP5\/ORP8 localize to endoplasmic reticulum-mitochondria contacts and are involved in mitochondrial function. EMBO Rep. 2016;17:800\u201310.","journal-title":"EMBO Rep"},{"key":"2438_CR32","first-page":"1","volume":"8","author":"VM Olkkonen","year":"2015","unstructured":"Olkkonen VM. OSBP-related protein family in lipid transport over membrane contact sites. Lipid Insights. 2015;8:1\u20139.","journal-title":"Lipid Insights"},{"key":"2438_CR33","doi-asserted-by":"crossref","unstructured":"Lemmon MA. Pleckstrin homology (PH) domains and phosphoinositides. In: Biochemical Society Symposium. 2007. pp. 81\u201393.","DOI":"10.1042\/BSS2007c08"},{"key":"2438_CR34","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/nature03923","volume":"437","author":"YJ Im","year":"2005","unstructured":"Im YJ, Raychaudhuri S, Prinz WA, Hurley JH. Structural mechanism for sterol sensing and transport by OSBP-related proteins. Nature. 2005;437:154\u20138.","journal-title":"Nature"},{"key":"2438_CR35","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1083\/jcb.116.2.307","volume":"116","author":"ND Ridgway","year":"1992","unstructured":"Ridgway ND, Dawson PA, Ho YK, Brown MS, Goldstein JL. Translocation of oxysterol binding protein to Golgi apparatus triggered by ligand binding. J Cell Biol. 1992;116:307\u201319.","journal-title":"J Cell Biol"},{"key":"2438_CR36","doi-asserted-by":"publisher","first-page":"9294","DOI":"10.1073\/pnas.0503596102","volume":"102","author":"J Zhong","year":"2005","unstructured":"Zhong J, Gastaminza P, Cheng G, Kapadia S, Kato T, Burton DR, Wieland SF, Uprichard SL, Wakita T, Chisari FV. Robust hepatitis C virus infection in vitro. Proc Natl Acad Sci USA. 2005;102:9294\u20139.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2438_CR37","doi-asserted-by":"publisher","first-page":"5697","DOI":"10.1128\/JVI.00146-12","volume":"86","author":"P Shukla","year":"2012","unstructured":"Shukla P, Nguyen HT, Faulk K, Mather K, Torian U, Engle RE, Emerson SU. Adaptation of a genotype 3 hepatitis E virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination. J Virol. 2012;86:5697\u2013707.","journal-title":"J Virol"},{"key":"2438_CR38","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.3390\/v11111050","volume":"11","author":"S Lin","year":"2019","unstructured":"Lin S, Yang Y, Nan Y, Ma Z, Yang L, Zhang YJ. The capsid protein of hepatitis E virus inhibits interferon induction via its N-terminal arginine-rich motif. Viruses. 2019;11:1050.","journal-title":"Viruses"},{"key":"2438_CR39","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00072-18","author":"L Yang","year":"2018","unstructured":"Yang L, Wang R, Yang S, Ma Z, Lin S, Nan Y, Li Q, Tang Q, Zhang YJ. Karyopherin alpha 6 is required for replication of porcine reproductive and respiratory syndrome virus and Zika virus. J Virol. 2018. https:\/\/doi.org\/10.1128\/JVI.00072-18.","journal-title":"J Virol"},{"key":"2438_CR40","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1126\/science.273.5276.797","volume":"273","author":"NB Cole","year":"1996","unstructured":"Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin M, Lippincott-Schwartz J. Diffusional mobility of Golgi proteins in membranes of living cells. Science. 1996;273:797\u2013801.","journal-title":"Science"},{"key":"2438_CR41","doi-asserted-by":"publisher","first-page":"6375","DOI":"10.1128\/JVI.02571-08","volume":"83","author":"H Kannan","year":"2009","unstructured":"Kannan H, Fan S, Patel D, Bossis I, Zhang YJ. The hepatitis E virus open reading frame 3 product interacts with microtubules and interferes with their dynamics. J Virol. 2009;83:6375\u201382.","journal-title":"J Virol"},{"key":"2438_CR42","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1038\/s41467-020-14957-y","volume":"11","author":"P Gee","year":"2020","unstructured":"Gee P, Lung MSY, Okuzaki Y, Sasakawa N, Iguchi T, Makita Y, Hozumi H, Miura Y, Yang LF, Iwasaki M, et al. Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping. Nat Commun. 2020;11:1334.","journal-title":"Nat Commun"},{"key":"2438_CR43","doi-asserted-by":"publisher","first-page":"8696","DOI":"10.1128\/JVI.01228-14","volume":"88","author":"Y Nan","year":"2014","unstructured":"Nan Y, Ma Z, Wang R, Yu Y, Kannan H, Fredericksen B, Zhang YJ. Enhancement of interferon induction by ORF3 product of hepatitis E virus. J Virol. 2014;88:8696\u2013705.","journal-title":"J Virol"},{"key":"2438_CR44","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w","author":"J Abramson","year":"2024","unstructured":"Abramson J, Adler J, Dunger J, Evans R, Green T, Pritzel A, Ronneberger O, Willmore L, Ballard AJ, Bambrick J, et al. Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature. 2024. https:\/\/doi.org\/10.1038\/s41586-024-07487-w.","journal-title":"Nature"},{"key":"2438_CR45","doi-asserted-by":"publisher","first-page":"11924","DOI":"10.1128\/JVI.01935-14","volume":"88","author":"Y Nan","year":"2014","unstructured":"Nan Y, Yu Y, Ma Z, Khattar SK, Fredericksen B, Zhang YJ. Hepatitis E virus inhibits type I interferon induction by ORF1 products. J Virol. 2014;88:11924\u201332.","journal-title":"J Virol"},{"issue":"220\u2013234","key":"2438_CR46","first-page":"e228","volume":"49","author":"D Jamecna","year":"2019","unstructured":"Jamecna D, Polidori J, Mesmin B, Dezi M, Levy D, Bigay J, Antonny B. An intrinsically disordered region in OSBP acts as an entropic barrier to control protein dynamics and orientation at membrane contact sites. Dev Cell. 2019;49(220\u2013234):e228.","journal-title":"Dev Cell"},{"key":"2438_CR47","doi-asserted-by":"publisher","first-page":"673509","DOI":"10.3389\/fmicb.2021.673509","volume":"12","author":"K Avula","year":"2021","unstructured":"Avula K, Singh B, Kumar PV, Syed GH. Role of lipid transfer proteins (LTPs) in the viral life cycle. Front Microbiol. 2021;12:673509.","journal-title":"Front Microbiol"},{"key":"2438_CR48","doi-asserted-by":"publisher","first-page":"286","DOI":"10.3389\/fmicb.2019.00286","volume":"10","author":"Z Zhang","year":"2019","unstructured":"Zhang Z, He G, Filipowicz NA, Randall G, Belov GA, Kopek BG, Wang X. Host lipids in positive-strand RNA virus genome replication. Front Microbiol. 2019;10:286.","journal-title":"Front Microbiol"},{"key":"2438_CR49","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1002\/hep.22197","volume":"47","author":"Y Nahmias","year":"2008","unstructured":"Nahmias Y, Goldwasser J, Casali M, van Poll D, Wakita T, Chung RT, Yarmush ML. Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin. Hepatology (Baltimore, MD). 2008;47:1437\u201345.","journal-title":"Hepatology (Baltimore, MD)"},{"key":"2438_CR50","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1016\/j.cell.2010.03.050","volume":"141","author":"NY Hsu","year":"2010","unstructured":"Hsu NY, Ilnytska O, Belov G, Santiana M, Chen YH, Takvorian PM, Pau C, van der Schaar H, Kaushik-Basu N, Balla T, et al. Viral reorganization of the secretory pathway generates distinct organelles for RNA replication. Cell. 2010;141:799\u2013811.","journal-title":"Cell"},{"key":"2438_CR51","doi-asserted-by":"publisher","first-page":"9237","DOI":"10.1128\/JVI.00958-09","volume":"83","author":"Y Amako","year":"2009","unstructured":"Amako Y, Sarkeshik A, Hotta H, Yates J 3rd, Siddiqui A. Role of oxysterol binding protein in hepatitis C virus infection. J Virol. 2009;83:9237\u201346.","journal-title":"J Virol"},{"key":"2438_CR52","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1053\/j.gastro.2014.02.002","volume":"146","author":"H Wang","year":"2014","unstructured":"Wang H, Perry JW, Lauring AS, Neddermann P, De Francesco R, Tai AW. Oxysterol-binding protein is a phosphatidylinositol 4-kinase effector required for HCV replication membrane integrity and cholesterol trafficking. Gastroenterology. 2014;146:1373\u201385.","journal-title":"Gastroenterology"},{"key":"2438_CR53","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01952-17","author":"K Ishikawa-Sasaki","year":"2018","unstructured":"Ishikawa-Sasaki K, Nagashima S, Taniguchi K, Sasaki J. Model of OSBP-mediated cholesterol supply to Aichi virus RNA replication sites involving protein-protein interactions among viral proteins, ACBD3, OSBP, VAP-A\/B, and SAC1. J Virol. 2018. https:\/\/doi.org\/10.1128\/jvi.01952-17.","journal-title":"J Virol"},{"key":"2438_CR54","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1016\/j.bbrc.2013.12.052","volume":"443","author":"M Iwamoto","year":"2014","unstructured":"Iwamoto M, Watashi K, Tsukuda S, Aly HH, Fukasawa M, Fujimoto A, Suzuki R, Aizaki H, Ito T, Koiwai O, et al. Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP. Biochem Biophys Res Commun. 2014;443:808\u201313.","journal-title":"Biochem Biophys Res Commun"},{"key":"2438_CR55","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.virusres.2015.12.007","volume":"213","author":"NK Ojha","year":"2016","unstructured":"Ojha NK, Lole KS. Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network. Virus Res. 2016;213:195\u2013204.","journal-title":"Virus Res"},{"key":"2438_CR56","doi-asserted-by":"publisher","first-page":"828636","DOI":"10.3389\/fmicb.2022.828636","volume":"13","author":"K Metzger","year":"2022","unstructured":"Metzger K, Bentaleb C, Hervouet K, Alexandre V, Montpellier C, Saliou JM, Ferrie M, Camuzet C, Rouille Y, Lecoeur C, et al. Processing and subcellular localization of the hepatitis E virus replicase: identification of candidate viral factories. Front Microbiol. 2022;13:828636.","journal-title":"Front Microbiol"},{"key":"2438_CR57","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00459-19","author":"D Szkolnicka","year":"2019","unstructured":"Szkolnicka D, Pollan A, Da Silva N, Oechslin N, Gouttenoire J, Moradpour D. Recombinant hepatitis E viruses harboring tags in the ORF1 protein. J Virol. 2019. https:\/\/doi.org\/10.1128\/JVI.00459-19.","journal-title":"J Virol"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-024-02438-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12985-024-02438-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-024-02438-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:02:31Z","timestamp":1721667751000},"score":1,"resource":{"primary":{"URL":"https:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/s12985-024-02438-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,22]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["2438"],"URL":"https:\/\/doi.org\/10.1186\/s12985-024-02438-3","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4079307\/v1","asserted-by":"object"}]},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,22]]},"assertion":[{"value":"5 March 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"161"}}