{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T03:51:06Z","timestamp":1793677866975,"version":"4.5.1"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"DOI":"10.1186\/s12985-024-02516-6","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T16:01:51Z","timestamp":1727798511000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative pathogenicity of influenza virus-induced pneumonia mouse model following intranasal and aerosolized intratracheal inoculation"],"prefix":"10.1186","volume":"21","author":[{"given":"Xiu-Yu","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Yu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Xi","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zai-Qing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Sheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Er-Hei","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,1]]},"reference":[{"issue":"1","key":"2516_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41572-018-0002-y","volume":"4","author":"F Krammer","year":"2018","unstructured":"Krammer F, et al. Influenza Nat Rev Dis Primers. 2018;4(1):3.","journal-title":"Influenza Nat Rev Dis Primers"},{"issue":"12","key":"2516_CR2","doi-asserted-by":"publisher","first-page":"4715","DOI":"10.1016\/j.apsb.2023.08.010","volume":"13","author":"C Liu","year":"2023","unstructured":"Liu C, et al. Emerging drug design strategies in anti-influenza drug discovery. Acta Pharm Sin B. 2023;13(12):4715\u201332.","journal-title":"Acta Pharm Sin B"},{"issue":"11","key":"2516_CR3","doi-asserted-by":"publisher","first-page":"Itc161","DOI":"10.7326\/AITC202111160","volume":"174","author":"TM Uyeki","year":"2021","unstructured":"Uyeki TM, Influenza. Ann Intern Med. 2021;174(11):Itc161\u201376.","journal-title":"Ann Intern Med"},{"issue":"1","key":"2516_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.rr7101a1","volume":"71","author":"LA Grohskopf","year":"2022","unstructured":"Grohskopf LA, et al. Prevention and Control of Seasonal Influenza with vaccines: recommendations of the Advisory Committee on Immunization Practices - United States, 2022-23 influenza season. MMWR Recomm Rep. 2022;71(1):1\u201328.","journal-title":"MMWR Recomm Rep"},{"issue":"15","key":"2516_CR5","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1001\/jama.2010.479","volume":"303","author":"AM Siston","year":"2010","unstructured":"Siston AM, et al. Pandemic 2009 influenza A(H1N1) virus illness among pregnant women in the United States. JAMA. 2010;303(15):1517\u201325.","journal-title":"JAMA"},{"issue":"1","key":"2516_CR6","doi-asserted-by":"publisher","first-page":"1742","DOI":"10.1080\/22221751.2022.2093135","volume":"11","author":"WWY Tso","year":"2022","unstructured":"Tso WWY, et al. Severity of SARS-CoV-2 Omicron BA.2 infection in unvaccinated hospitalized children: comparison to influenza and parainfluenza infections. Emerg Microbes Infect. 2022;11(1):1742\u201350.","journal-title":"Emerg Microbes Infect"},{"issue":"10127","key":"2516_CR7","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1016\/S0140-6736(17)33293-2","volume":"391","author":"AD Iuliano","year":"2018","unstructured":"Iuliano AD, et al. Estimates of global seasonal influenza-associated respiratory mortality: a modelling study. Lancet. 2018;391(10127):1285\u2013300.","journal-title":"Lancet"},{"key":"2516_CR8","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.virol.2015.02.041","volume":"481","author":"JA Belser","year":"2015","unstructured":"Belser JA, et al. Comparison of traditional intranasal and aerosol inhalation inoculation of mice with influenza A viruses. Virology. 2015;481:107\u201312.","journal-title":"Virology"},{"issue":"1","key":"2516_CR9","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1038\/s41426-018-0072-7","volume":"7","author":"R El-Shesheny","year":"2018","unstructured":"El-Shesheny R, et al. Replication and pathogenic potential of influenza a virus subtypes H3, H7, and H15 from free-range ducks in Bangladesh in mammals. Emerg Microbes Infect. 2018;7(1):70.","journal-title":"Emerg Microbes Infect"},{"issue":"8","key":"2516_CR10","first-page":"e50","volume":"2","author":"Z Zhu","year":"2013","unstructured":"Zhu Z, et al. Infection of inbred BALB\/c and C57BL\/6 and outbred Institute of Cancer Research mice with the emerging H7N9 avian influenza virus. Emerg Microbes Infect. 2013;2(8):e50.","journal-title":"Emerg Microbes Infect"},{"issue":"3","key":"2516_CR11","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1080\/01926230701302558","volume":"35","author":"JP Buchweitz","year":"2007","unstructured":"Buchweitz JP, Harkema JR, Kaminski NE. Time-dependent airway epithelial and inflammatory cell responses induced by influenza virus A\/PR\/8\/34 in C57BL\/6 mice. Toxicol Pathol. 2007;35(3):424\u201335.","journal-title":"Toxicol Pathol"},{"key":"2516_CR12","doi-asserted-by":"crossref","unstructured":"Rodriguez L, Nogales A, Mart\u00ednez-Sobrido L. Influenza a Virus studies in a mouse model of infection. J Vis Exp, 2017(127).","DOI":"10.3791\/55898"},{"key":"2516_CR13","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.jviromet.2014.09.004","volume":"209","author":"L Morales-Nebreda","year":"2014","unstructured":"Morales-Nebreda L, et al. Intratracheal administration of influenza virus is superior to intranasal administration as a model of acute lung injury. J Virol Methods. 2014;209:116\u201320.","journal-title":"J Virol Methods"},{"issue":"2","key":"2516_CR14","doi-asserted-by":"publisher","first-page":"L262","DOI":"10.1152\/ajplung.00046.2023","volume":"325","author":"Y Seo","year":"2023","unstructured":"Seo Y, et al. Optimizing anesthesia and delivery approaches for dosing into lungs of mice. Am J Physiol Lung Cell Mol Physiol. 2023;325(2):L262\u20139.","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2516_CR15","doi-asserted-by":"crossref","unstructured":"Zhao Y et al. Time-Course Transcriptome Analysis of the lungs of mice challenged with aerosols of Methicillin-resistant Staphylococcus aureus USA300 clone reveals inflammatory balance. Biomolecules, 2023. 13(2).","DOI":"10.3390\/biom13020347"},{"key":"2516_CR16","doi-asserted-by":"publisher","first-page":"267","DOI":"10.2217\/fmb-2023-0009","volume":"18","author":"Y Wang","year":"2023","unstructured":"Wang Y, et al. Mechanism of pulmonary plague biphasic syndrome: inhibition or activation of NF-\u03baB signaling pathway. Future Microbiol. 2023;18:267\u201386.","journal-title":"Future Microbiol"},{"issue":"1","key":"2516_CR17","doi-asserted-by":"publisher","first-page":"2350","DOI":"10.1080\/22221751.2022.2122577","volume":"11","author":"HY Qiu","year":"2022","unstructured":"Qiu HY, et al. Aerosolized Zika virus infection in Guinea pigs. Emerg Microbes Infect. 2022;11(1):2350\u20138.","journal-title":"Emerg Microbes Infect"},{"key":"2516_CR18","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.toxlet.2020.11.012","volume":"337","author":"Z Jiao","year":"2021","unstructured":"Jiao Z, et al. Time-course transcriptome analysis of lungs from mice exposed to ricin by intratracheal inoculation. Toxicol Lett. 2021;337:57\u201367.","journal-title":"Toxicol Lett"},{"key":"2516_CR19","doi-asserted-by":"publisher","first-page":"3307","DOI":"10.3389\/fmicb.2018.03307","volume":"9","author":"AJ Eisfeld","year":"2018","unstructured":"Eisfeld AJ, et al. C57BL\/6J and C57BL\/6NJ mice are differentially susceptible to inflammation-Associated Disease caused by Influenza A Virus. Front Microbiol. 2018;9:3307.","journal-title":"Front Microbiol"},{"key":"2516_CR20","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.intimp.2018.03.029","volume":"59","author":"N Song","year":"2018","unstructured":"Song N, et al. C5a receptor1 inhibition alleviates influenza virus-induced acute lung injury. Int Immunopharmacol. 2018;59:12\u201320.","journal-title":"Int Immunopharmacol"},{"issue":"4 Suppl","key":"2516_CR21","doi-asserted-by":"publisher","first-page":"s7","DOI":"10.1177\/0192623312438738","volume":"40","author":"PC Mann","year":"2012","unstructured":"Mann PC, et al. International harmonization of toxicologic pathology nomenclature: an overview and review of basic principles. Toxicol Pathol. 2012;40(4 Suppl):s7\u201313.","journal-title":"Toxicol Pathol"},{"issue":"1","key":"2516_CR22","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","volume":"162","author":"P Chomczynski","year":"1987","unstructured":"Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156\u20139.","journal-title":"Anal Biochem"},{"key":"2516_CR23","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.envres.2018.11.014","volume":"169","author":"Q Deng","year":"2019","unstructured":"Deng Q, et al. Particle deposition in the human lung: Health implications of particulate matter from different sources. Environ Res. 2019;169:237\u201345.","journal-title":"Environ Res"},{"issue":"11","key":"2516_CR24","doi-asserted-by":"publisher","first-page":"2201","DOI":"10.1016\/j.jbiomech.2015.11.029","volume":"49","author":"PG Koullapis","year":"2016","unstructured":"Koullapis PG, et al. Particle deposition in a realistic geometry of the human conducting airways: effects of inlet velocity profile, inhalation flowrate and electrostatic charge. J Biomech. 2016;49(11):2201\u201312.","journal-title":"J Biomech"},{"issue":"3","key":"2516_CR25","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/ja.2013.132","volume":"67","author":"R Sugamata","year":"2014","unstructured":"Sugamata R, et al. Leucomycin A3, a 16-membered macrolide antibiotic, inhibits influenza a virus infection and disease progression. J Antibiot (Tokyo). 2014;67(3):213\u201322.","journal-title":"J Antibiot (Tokyo)"},{"key":"2516_CR26","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.abb.2018.01.004","volume":"640","author":"Y Aratani","year":"2018","unstructured":"Aratani Y. Myeloperoxidase: its role for host defense, inflammation, and neutrophil function. Arch Biochem Biophys. 2018;640:47\u201352.","journal-title":"Arch Biochem Biophys"},{"key":"2516_CR27","doi-asserted-by":"publisher","first-page":"1041552","DOI":"10.3389\/fimmu.2022.1041552","volume":"13","author":"S Kozlovski","year":"2022","unstructured":"Kozlovski S, et al. ICAMs are dispensable for influenza clearance and anti-viral humoral and cellular immunity. Front Immunol. 2022;13:1041552.","journal-title":"Front Immunol"},{"issue":"43","key":"2516_CR28","doi-asserted-by":"publisher","first-page":"4995","DOI":"10.1016\/j.vaccine.2013.06.071","volume":"31","author":"JH Kreijtz","year":"2013","unstructured":"Kreijtz JH, et al. Low pathogenic avian influenza A(H7N9) virus causes high mortality in ferrets upon intratracheal challenge: a model to study intervention strategies. Vaccine. 2013;31(43):4995\u20139.","journal-title":"Vaccine"},{"issue":"1","key":"2516_CR29","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.ajpath.2011.03.026","volume":"179","author":"R Bodewes","year":"2011","unstructured":"Bodewes R, et al. Pathogenesis of Influenza A\/H5N1 virus infection in ferrets differs between intranasal and intratracheal routes of inoculation. Am J Pathol. 2011;179(1):30\u20136.","journal-title":"Am J Pathol"},{"issue":"5","key":"2516_CR30","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1007\/s12275-010-0120-z","volume":"48","author":"D Kwon","year":"2010","unstructured":"Kwon D, et al. Replication and pathogenesis of the pandemic (H1N1) 2009 influenza virus in mammalian models. J Microbiol. 2010;48(5):657\u201362.","journal-title":"J Microbiol"},{"issue":"2","key":"2516_CR31","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.micinf.2010.10.004","volume":"13","author":"MD Tate","year":"2011","unstructured":"Tate MD, Brooks AG, Reading PC. Correlation between sialic acid expression and infection of murine macrophages by different strains of influenza virus. Microbes Infect. 2011;13(2):202\u20137.","journal-title":"Microbes Infect"},{"key":"2516_CR32","doi-asserted-by":"crossref","unstructured":"Zhao C, Pu J. Influence of host sialic acid receptors structure on the host specificity of Influenza viruses. Viruses, 2022. 14(10).","DOI":"10.3390\/v14102141"},{"issue":"12","key":"2516_CR33","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1080\/17460913.2024.2355738","volume":"19","author":"J Wang","year":"2024","unstructured":"Wang J, et al. Mice fatal pneumonia model induced by less-virulent Streptococcus pneumoniae via intratracheal aerosolization. Future Microbiol. 2024;19(12):1055\u201370.","journal-title":"Future Microbiol"},{"issue":"11","key":"2516_CR34","doi-asserted-by":"publisher","first-page":"1918","DOI":"10.1002\/jmv.25550","volume":"91","author":"XY Hao","year":"2019","unstructured":"Hao XY, et al. Establishment of BALB\/C mouse models of influenza A H1N1 aerosol inhalation. J Med Virol. 2019;91(11):1918\u201329.","journal-title":"J Med Virol"},{"key":"2516_CR35","doi-asserted-by":"publisher","first-page":"74","DOI":"10.3389\/fcimb.2012.00074","volume":"2","author":"LE Bowen","year":"2012","unstructured":"Bowen LE, et al. Development of a murine nose-only inhalation model of influenza: comparison of disease caused by instilled and inhaled A\/PR\/8\/34. Front Cell Infect Microbiol. 2012;2:74.","journal-title":"Front Cell Infect Microbiol"},{"issue":"3","key":"2516_CR36","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.ajpath.2014.11.012","volume":"185","author":"LC Wiersma","year":"2015","unstructured":"Wiersma LC, et al. Pathogenesis of infection with 2009 pandemic H1N1 influenza virus in isogenic guinea pigs after intranasal or intratracheal inoculation. Am J Pathol. 2015;185(3):643\u201350.","journal-title":"Am J Pathol"},{"issue":"8","key":"2516_CR37","doi-asserted-by":"publisher","first-page":"e6662","DOI":"10.1371\/journal.pone.0006662","volume":"4","author":"A De Vleeschauwer","year":"2009","unstructured":"De Vleeschauwer A, et al. Comparative pathogenesis of an avian H5N2 and a swine H1N1 influenza virus in pigs. PLoS ONE. 2009;4(8):e6662.","journal-title":"PLoS ONE"},{"issue":"3","key":"2516_CR38","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1177\/0300985811429812","volume":"49","author":"JM van den Brand","year":"2012","unstructured":"van den Brand JM, et al. Modification of the ferret model for pneumonia from seasonal human influenza a virus infection. Vet Pathol. 2012;49(3):562\u20138.","journal-title":"Vet Pathol"},{"key":"2516_CR39","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.virusres.2018.05.023","volume":"255","author":"J Luo","year":"2018","unstructured":"Luo J, et al. Comparative pathogenesis of H3N2 canine influenza virus in beagle dogs challenged by intranasal and intratracheal inoculation. Virus Res. 2018;255:147\u201353.","journal-title":"Virus Res"},{"issue":"3","key":"2516_CR40","doi-asserted-by":"publisher","first-page":"e1010395","DOI":"10.1371\/journal.ppat.1010395","volume":"18","author":"J Corry","year":"2022","unstructured":"Corry J, et al. Infiltration of inflammatory macrophages and neutrophils and widespread pyroptosis in lung drive influenza lethality in nonhuman primates. PLoS Pathog. 2022;18(3):e1010395.","journal-title":"PLoS Pathog"},{"issue":"2","key":"2516_CR41","doi-asserted-by":"publisher","first-page":"e76","DOI":"10.1016\/j.ijid.2012.06.006","volume":"17","author":"J Bordon","year":"2013","unstructured":"Bordon J, et al. Understanding the roles of cytokines and neutrophil activity and neutrophil apoptosis in the protective versus deleterious inflammatory response in pneumonia. Int J Infect Dis. 2013;17(2):e76\u201383.","journal-title":"Int J Infect Dis"},{"issue":"1","key":"2516_CR42","doi-asserted-by":"publisher","first-page":"3422","DOI":"10.1038\/s41467-019-11249-y","volume":"10","author":"BM Tang","year":"2019","unstructured":"Tang BM, et al. Neutrophils-related host factors associated with severe disease and fatality in patients with influenza infection. Nat Commun. 2019;10(1):3422.","journal-title":"Nat Commun"},{"issue":"6","key":"2516_CR43","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1002\/JLB.1MR1221-345R","volume":"111","author":"Y Zhang","year":"2022","unstructured":"Zhang Y, et al. Neutrophil subsets and their differential roles in viral respiratory diseases. J Leukoc Biol. 2022;111(6):1159\u201373.","journal-title":"J Leukoc Biol"},{"issue":"1","key":"2516_CR44","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.ajpath.2011.03.013","volume":"179","author":"T Narasaraju","year":"2011","unstructured":"Narasaraju T, et al. Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis. Am J Pathol. 2011;179(1):199\u2013210.","journal-title":"Am J Pathol"},{"issue":"7","key":"2516_CR45","doi-asserted-by":"publisher","first-page":"819","DOI":"10.15252\/msb.20156123","volume":"11","author":"HB Schiller","year":"2015","unstructured":"Schiller HB, et al. Time- and compartment-resolved proteome profiling of the extracellular niche in lung injury and repair. Mol Syst Biol. 2015;11(7):819.","journal-title":"Mol Syst Biol"},{"issue":"5","key":"2516_CR46","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1016\/j.copbio.2013.04.011","volume":"24","author":"KC Clause","year":"2013","unstructured":"Clause KC, Barker TH. Extracellular matrix signaling in morphogenesis and repair. Curr Opin Biotechnol. 2013;24(5):830\u20133.","journal-title":"Curr Opin Biotechnol"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-024-02516-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12985-024-02516-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-024-02516-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T16:03:48Z","timestamp":1727798628000},"score":1,"resource":{"primary":{"URL":"https:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/s12985-024-02516-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,1]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["2516"],"URL":"https:\/\/doi.org\/10.1186\/s12985-024-02516-6","relation":{},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,1]]},"assertion":[{"value":"27 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"240"}}