{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T05:55:04Z","timestamp":1793858104650,"version":"4.6.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32225050"],"award-info":[{"award-number":["32225050"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"DOI":"10.1186\/s12985-025-02716-8","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T02:07:54Z","timestamp":1743732474000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification and characterization of a novel reovirus strain isolated fromgrass carp (Ctenopharyngodon idella)"],"prefix":"10.1186","volume":"22","author":[{"given":"Weiguang","family":"Kong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyi","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjie","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"2716_CR1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.fsi.2018.09.003","volume":"83","author":"H Su","year":"2018","unstructured":"Su H, Su JG. Cyprinid viral diseases and vaccine development. Fish Shellfish Immunol. 2018;83:84\u201395.","journal-title":"Fish Shellfish Immunol"},{"key":"2716_CR2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s10126-023-10225-4","volume":"25","author":"CC Yu","year":"2023","unstructured":"Yu CC, Wu ML, Jiang YC, Xu XY, Li JL, Shen YB. Transcriptome analysis of the spleen provides insight into the immune regulation of GCRV resistance in grass carp (Ctenopharyngodon idella). Mar Biotechnol. 2023;25:557\u201366.","journal-title":"Mar Biotechnol"},{"key":"2716_CR3","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.fsi.2020.08.047","volume":"106","author":"J Liu","year":"2020","unstructured":"Liu J, Yan YJ, Yan J, Wang JT, Wei J, Xiao J, et al. Multi-omics analysis revealed crucial genes and pathways associated with black carp antiviral innate immunity. Fish Shellfish Immunol. 2020;106:724\u201332.","journal-title":"Fish Shellfish Immunol"},{"issue":"22","key":"2716_CR4","doi-asserted-by":"crossref","first-page":"12466","DOI":"10.1128\/JVI.02333-12","volume":"86","author":"Q Wang","year":"2012","unstructured":"Wang Q, Zeng WW, Liu C, Zhang C, Wang YY, Shi CB, et al. Complete genome sequence of a reovirus isolated from grass carp, indicating different genotypes of GCRV in China. J Virol. 2012;86(22):12466.","journal-title":"J Virol"},{"key":"2716_CR5","doi-asserted-by":"crossref","first-page":"128454","DOI":"10.1016\/j.ijbiomac.2023.128454","volume":"256","author":"XY Liang","year":"2024","unstructured":"Liang XY, Wang Q, Wang HY, Wang XY, Chu PF, Yang C, et al. Grass carp superoxide dismutases exert antioxidant function and inhibit autophagy to promote grass carp reovirus (GCRV) replication. Int J Biol Macromol. 2024;256:128454.","journal-title":"Int J Biol Macromol"},{"issue":"2","key":"2716_CR6","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.fsi.2014.10.004","volume":"41","author":"XB Yu","year":"2014","unstructured":"Yu XB, Liu GL, Zhu B, Hao K, Ling F, Wang GX. In vitro immunocompetence of two compounds isolated from Polygala tenuifolia and development of resistance against grass carp reovirus (GCRV) and Dactylogyrus intermedius in respective host. Fish Shellfish Immunol. 2014;41(2):541\u20138.","journal-title":"Fish Shellfish Immunol"},{"issue":"1","key":"2716_CR7","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.dci.2014.07.006","volume":"47","author":"B Wang","year":"2014","unstructured":"Wang B, Zhang YB, Liu TK, Shi J, Sun F, Gui JF. Fish Viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway. Dev Comp Immunol. 2014;47(1):140\u20139.","journal-title":"Dev Comp Immunol"},{"issue":"8","key":"2716_CR8","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1111\/j.1095-8649.2010.02624.x","volume":"76","author":"C Yang","year":"2010","unstructured":"Yang C, Su J. Molecular identification and expression analysis of Toll-like receptor 3 in common carp Cyprinus Carpio. J Fish Biol. 2010;76(8):1926\u201339.","journal-title":"J Fish Biol"},{"key":"2716_CR9","doi-asserted-by":"crossref","first-page":"736133","DOI":"10.1016\/j.aquaculture.2020.736133","volume":"533","author":"JM Chen","year":"2021","unstructured":"Chen JM, Chang OQ, Li YY, Wang YY, Liu C, Yin JY, et al. Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II. Aquaculture. 2021;533:736133.","journal-title":"Aquaculture"},{"key":"2716_CR10","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.fsi.2021.07.014","volume":"117","author":"JM Chen","year":"2021","unstructured":"Chen JM, Li YY, Wang YY, Wu SY, Chang OQ, Yin JY, et al. Establishment of a rare minnow (Gobiocypris rarus) model for evaluation of experimental vaccines against a disease induced by grass carp reovirus genotype II. Fish Shellfish Immunol. 2021;117:53\u201361.","journal-title":"Fish Shellfish Immunol"},{"issue":"1","key":"2716_CR11","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1186\/s12864-017-3824-1","volume":"18","author":"LB He","year":"2017","unstructured":"He LB, Zhang AD, Pei YY, Chu PF, Li YM, Huang R, et al. Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing. BMC Genom. 2017;18(1):452.","journal-title":"BMC Genom"},{"issue":"11","key":"2716_CR12","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1007\/s00705-010-0753-6","volume":"155","author":"C Fan","year":"2010","unstructured":"Fan C, Shao L, Fang Q. Characterization of the nonstructural protein NS80 of grass carp reovirus. Arch Virol. 2010;155(11):1755\u201363.","journal-title":"Arch Virol"},{"issue":"9","key":"2716_CR13","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1007\/s00705-014-2007-5","volume":"159","author":"C Pei","year":"2014","unstructured":"Pei C, Ke F, Chen ZY, Zhang QY. Complete genome sequence and comparative analysis of grass carp reovirus strain 109 (GCReV-109) with other grass carp reovirus strains reveals no significant correlation with regional distribution. Arch Virol. 2014;159(9):2435\u201340.","journal-title":"Arch Virol"},{"key":"2716_CR14","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.virusres.2014.05.009","volume":"189","author":"J Ma","year":"2014","unstructured":"Ma J, Zeng LB, Fan YD, Zhou Y, Jiang N, Chen Q. Significant Inhibition of two different genotypes of grass carp reovirus in vitro using multiple ShRNAs expression vectors. Virus Res. 2014;189:47\u201355.","journal-title":"Virus Res"},{"issue":"6","key":"2716_CR15","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1111\/jwas.12326","volume":"47","author":"Q Wang","year":"2016","unstructured":"Wang Q, Zeng WW, Yin L, Wang YY, Liu C, Li YY, et al. Comparative study on physical-chemical and biological characteristics of grass carp reovirus from different genotypes. J World Aquacult Soc. 2016;47(6):862\u201372.","journal-title":"J World Aquacult Soc"},{"key":"2716_CR16","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-3-319-39196-0_5","volume":"940","author":"MG Mateu","year":"2016","unstructured":"Mateu MG. Assembly, engineering and applications of virus-based protein nanoparticles. Adv Exp Med Biol. 2016;940:83\u2013120.","journal-title":"Adv Exp Med Biol"},{"issue":"4","key":"2716_CR17","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1515\/pjvs-2016-0096","volume":"19","author":"HR Liang","year":"2016","unstructured":"Liang HR, Fu XZ, Li NQ, Liu LH, Lin Q, Li YG, et al. The distribution of different virulence grass carp reovirus strains in some neglected tissues. Pol J Vet Sci. 2016;19(4):763\u201370.","journal-title":"Pol J Vet Sci"},{"issue":"11","key":"2716_CR18","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.3390\/cells9112431","volume":"9","author":"PY Lozach","year":"2020","unstructured":"Lozach PY. Cell biology of viral infections. Cells. 2020;9(11):2431.","journal-title":"Cells"},{"issue":"3","key":"2716_CR19","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/mr.49.3.298-337.1985","volume":"49","author":"JW Moulder","year":"1985","unstructured":"Moulder JW. Comparative biology of intracellular parasitism. Microbiol Rev. 1985;49(3):298\u2013337.","journal-title":"Microbiol Rev"},{"issue":"20","key":"2716_CR20","doi-asserted-by":"crossref","first-page":"10932","DOI":"10.3390\/ijms222010932","volume":"22","author":"J B\u00f8gwald","year":"2021","unstructured":"B\u00f8gwald J, Dalmo RA. Protection of teleost fish against infectious diseases through oral administration of vaccines: update 2021. Int J Mol Sci. 2021;22(20):10932.","journal-title":"Int J Mol Sci"},{"key":"2716_CR21","first-page":"329","volume":"19","author":"BQ Liu","year":"2012","unstructured":"Liu BQ, Zeng WW, Wang Q, Zhang LS, Wang YY, Shi CB, et al. Development of a fluorescent quantitative polymerase chain reaction technique for detection of grass carp reovirus HZ08 strain. J Fish Sci China. 2012;19:329\u201335.","journal-title":"J Fish Sci China"},{"key":"2716_CR22","doi-asserted-by":"crossref","unstructured":"Fang Q, Zhang J, Zhang FX. Molecular Biology of Aquareoviruses, In: Fang, Q, editors. Aquareovirus Springer Singapore. 2021;39\u201375.","DOI":"10.1007\/978-981-16-1903-8_2"},{"key":"2716_CR23","doi-asserted-by":"crossref","first-page":"741243","DOI":"10.1016\/j.aquaculture.2024.741243","volume":"592","author":"CC Yu","year":"2024","unstructured":"Yu CC, Jiang YC, Zhang CY, Wu ML, Gui L, Xu XY, et al. Whole-genome resequencing of grass carp (Ctenopharyngodon idella) for genome-wide association study on GCRV resistance. Aquaculture. 2024;592:741243.","journal-title":"Aquaculture"},{"key":"2716_CR24","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1007\/s10499-022-00884-w","volume":"30","author":"H Mondal","year":"2022","unstructured":"Mondal H, Thomas J. A review on the recent advances and application of vaccines against fish pathogens in aquaculture. Aquacult Int. 2022;30:1971\u20132000.","journal-title":"Aquacult Int"},{"key":"2716_CR25","doi-asserted-by":"crossref","first-page":"5809","DOI":"10.1111\/are.16080","volume":"53","author":"S Lin","year":"2022","unstructured":"Lin S, Milardi M, Gao Y, Wong MH. Sustainable management of non-native grass carp as a protein source, weed\u2010control agent and sport fish. Aquac Res. 2022;53:5809\u201324.","journal-title":"Aquac Res"},{"key":"2716_CR26","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1186\/1743-422X-11-178","volume":"11","author":"HR Liang","year":"2014","unstructured":"Liang HR, Li YG, Zeng WW, Wang YY, Wang Q, Wu SQ. Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration. Virol J. 2014;11:178.","journal-title":"Virol J"},{"key":"2716_CR27","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.virs.2023.04.006","volume":"38","author":"R Jiang","year":"2023","unstructured":"Jiang R, Zhang J, Liao ZW, Zhu WT, Su H, Zhang YG, et al. Temperature-regulated type II grass carp reovirus establishes latent infection in Ctenopharyngodon Idella brain. Virol Sin. 2023;38:440\u20137.","journal-title":"Virol Sin"},{"key":"2716_CR28","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.3390\/vaccines11081373","volume":"11","author":"QQ Tian","year":"2023","unstructured":"Tian QQ, Huo XC, Liu Q, Yang CR, Zhang YG, Su JG. VP4\/VP56\/VP35 Virus-like particles effectively protect grass carp (Ctenopharyngodon idella) against GCRV-II infection. Vaccines. 2023;11:1373.","journal-title":"Vaccines"},{"key":"2716_CR29","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3390\/v14010030","volume":"14","author":"Y Gao","year":"2021","unstructured":"Gao Y, Huo XC, Wang ZS, Yuan GL, Liu XL, Ai TS, et al. Oral administration of Bacillus subtilis subunit vaccine significantly enhances the immune protection of grass carp against GCRV-II infection. Viruses. 2021;14:30.","journal-title":"Viruses"},{"key":"2716_CR30","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.chom.2013.03.010","volume":"13","author":"OO Koyuncu","year":"2013","unstructured":"Koyuncu OO, Hogue IB, Enquist LW. Virus infections in the nervous system. Cell Host Microbe. 2013;13:379\u201393.","journal-title":"Cell Host Microbe"},{"key":"2716_CR31","doi-asserted-by":"crossref","first-page":"105292","DOI":"10.1016\/j.micpath.2021.105292","volume":"161","author":"V Pandey","year":"2021","unstructured":"Pandey V, Bhat RAH, Chandra S, Tandel RS, Dubey MK, Sharma P, et al. Clinical signs, lethal dose and histopathological lesions in grass carp, Ctenopharyngodon Idella experimentally infected with Edwardsiella Tarda. Microb Pathog. 2021;161:105292.","journal-title":"Microb Pathog"},{"issue":"21","key":"2716_CR32","doi-asserted-by":"crossref","first-page":"15501","DOI":"10.3390\/ijms242115501","volume":"24","author":"WG Kong","year":"2023","unstructured":"Kong WG, Ding GY, Yang P, Li YQ, Cheng GF, Cai C, et al. Comparative transcriptomic analysis revealed potential differential mechanisms of grass carp reovirus pathogenicity. Int J Mol Sci. 2023;24(21):15501.","journal-title":"Int J Mol Sci"},{"key":"2716_CR33","doi-asserted-by":"crossref","first-page":"848958","DOI":"10.3389\/fimmu.2022.848958","volume":"13","author":"C Xu","year":"2022","unstructured":"Xu C, Qiao MH, Huo XC, Liao ZW, Su JG. An oral microencapsulated vaccine loaded by sodium alginate effectively enhances protection against GCRV infection in grass carp (Ctenopharyngodon idella). Front Immunol. 2022;13:848958.","journal-title":"Front Immunol"},{"key":"2716_CR34","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.tim.2014.08.005","volume":"22","author":"M Ciechonska","year":"2014","unstructured":"Ciechonska M, Duncan R, Reovirus. FAST proteins: virus-encoded cellular fusogens. Trends Microbiol. 2014;22:715\u201324.","journal-title":"Trends Microbiol"},{"key":"2716_CR35","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/2193-1801-3-190","volume":"3","author":"XY Yan","year":"2014","unstructured":"Yan XY, Wang Y, Xiong LF, Jian JC, Wu ZH. Phylogenetic analysis of newly isolated grass carp reovirus. Springerplus. 2014;3:190.","journal-title":"Springerplus"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-025-02716-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12985-025-02716-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12985-025-02716-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T02:09:03Z","timestamp":1743732543000},"score":1,"resource":{"primary":{"URL":"https:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/s12985-025-02716-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,2]]},"references-count":35,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2716"],"URL":"https:\/\/doi.org\/10.1186\/s12985-025-02716-8","relation":{},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,2]]},"assertion":[{"value":"29 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experimental protocols were conducted in accordance with the Guiding Principles for the Keeping and Use of Laboratory Animals and were approved by the Institute of Hydrobiology, Chinese Academy of Sciences (permit number 2023-027).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"92"}}