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The brain vasculature is an important factor in many neonatal neurological disorders but there is a lack of diagnostic tools to evaluate the brain vascular dysfunction of neonates in the clinical setting. Measurement of blood\u2013brain barrier tight-junction (TJ)\u00a0proteins have shown promise as biomarkers for brain injury in the adult. Here we tested the biomarker potential of tight-junctions in the context of neonatal brain injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The levels of TJ-proteins (occluding, claudin-5, and zonula occludens protein 1) in both blood plasma and cerebrospinal fluid (CSF) as well as blood\u2013brain barrier function via\n                      <jats:sup>14<\/jats:sup>\n                      C-sucrose (342\u00a0Da) and Evans blue extravasation were measured in a hypoxia\/ischemia brain-injury model in neonatal rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Time-dependent changes of occludin and claudin-5 levels could be measured in blood and CSF after hypoxia\/ischemia with males generally having higher levels than females. The levels of claudin-5 in CSF correlated with the severity of the brain injury at 24\u00a0h post- hypoxia\/ischemia. Simultaneously, we detected early increase in blood\u2013brain barrier-permeability at 6 and 24\u00a0h after hypoxia\/ischemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Levels of circulating claudin-5 and occludin are increased after hypoxic\/ischemic brain injuries and blood\u2013brain barrier-impairment and have promise as early biomarkers for cerebral vascular dysfunction and as a tool for risk assessment of neonatal brain injuries.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12987-021-00240-9","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T14:54:11Z","timestamp":1612968851000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Circulating tight-junction proteins are potential biomarkers for blood\u2013brain barrier function in a model of neonatal hypoxic\/ischemic brain injury"],"prefix":"10.1186","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7165-3709","authenticated-orcid":false,"given":"E. 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