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A transcriptome analysis of HIBCPP cells following infection with\n                      <jats:italic>Nm<\/jats:italic>\n                      by massive analysis of cDNA ends (MACE) was done to further characterize the cellular response to infection of the barrier.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Interestingly, whereas\n                      <jats:italic>Nm<\/jats:italic>\n                      B and\n                      <jats:italic>Nm<\/jats:italic>\n                      C wild type strains required active Erk1\/2 and p38 pathways for infection, invasion by capsule-deficient mutants was independent of Erk1\/2 and, in case of the\n                      <jats:italic>Nm<\/jats:italic>\n                      B strain, of p38 activity. The transcriptome analysis of HIBCPP cells following infection with\n                      <jats:italic>Nm<\/jats:italic>\n                      demonstrated specific regulation of genes involved in the immune response dependent on Erk1\/2 signalling. Gene ontology (GO) analysis confirmed loss of MAPK signalling after Erk1\/2 inhibition and revealed an additional reduction of cellular responses including NF\u03baB and JAK-STAT signalling. Interestingly, GO terms related to TNF signalling and production of IL6 were lost specifically following Erk1\/2 inhibition during infection with wild type\n                      <jats:italic>Nm<\/jats:italic>\n                      , which correlated with the reduced infection rates by the wild type in absence of Erk1\/2 signalling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our data point towards a role of MAPK signalling during infection of the CP epithelium by\n                      <jats:italic>Nm<\/jats:italic>\n                      , which is strongly influenced by capsule expression, and affects infection rates as well as the host cell response.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12987-021-00288-7","type":"journal-article","created":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T13:02:27Z","timestamp":1638622947000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Capsule-dependent impact of MAPK signalling on host cell invasion and immune response during infection of the choroid plexus epithelium by Neisseria meningitidis"],"prefix":"10.1186","volume":"18","author":[{"given":"Rosanna","family":"Herold","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Scholtysik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selina","family":"Moroniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Schroten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-0259","authenticated-orcid":false,"given":"Christian","family":"Schwerk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,4]]},"reference":[{"key":"288_CR1","doi-asserted-by":"publisher","first-page":"B3","DOI":"10.1016\/j.vaccine.2011.12.062","volume":"30","author":"D Pace","year":"2012","unstructured":"Pace D, Pollard AJ. 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