{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:18:33Z","timestamp":1793261913556,"version":"4.5.0"},"reference-count":105,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"NIOSH Nanotechnology Research Center","award":["921043S"],"award-info":[{"award-number":["921043S"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["921043S"],"award-info":[{"award-number":["921043S"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["939051L"],"award-info":[{"award-number":["939051L"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["921043S"],"award-info":[{"award-number":["921043S"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["939051L"],"award-info":[{"award-number":["939051L"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["921043S"],"award-info":[{"award-number":["921043S"]}]},{"name":"NIOSH Nanotechnology Research Center","award":["939051L"],"award-info":[{"award-number":["939051L"]}]},{"name":"National Science Foundation","award":["1434503"],"award-info":[{"award-number":["1434503"]}]},{"DOI":"10.13039\/501100004919","name":"King Abdulaziz City for Science and Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004919","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Part Fibre Toxicol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Organomodified nanoclays (ONC), two-dimensional montmorillonite with organic coatings, are increasingly used to improve nanocomposite properties. However, little is known about pulmonary health risks along the nanoclay life cycle even with increased evidence of airborne particulate exposures in occupational environments. Recently, oropharyngeal aspiration exposure to pre- and post-incinerated ONC in mice caused low grade, persistent lung inflammation with a pro-fibrotic signaling response with unknown mode(s) of action. We hypothesized that the organic coating presence and incineration status of nanoclays determine the inflammatory cytokine secretary profile and cytotoxic response of macrophages. To test this hypothesis differentiated human macrophages (THP-1) were acutely exposed (0\u201320\u00a0\u00b5g\/cm<jats:sup>2<\/jats:sup>) to pristine, uncoated nanoclay (CloisNa), an ONC (Clois30B), their incinerated byproducts (I-CloisNa and I-Clois30B), and crystalline silica (CS) followed by cytotoxicity and inflammatory endpoints. Macrophages were co-exposed to lipopolysaccharide (LPS) or LPS-free medium to assess the role of priming the NF-\u03baB pathway in macrophage response to nanoclay treatment. Data were compared to inflammatory responses in male C57Bl\/6J mice following 30 and 300\u00a0\u00b5g\/mouse aspiration exposure to the same particles.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In LPS-free media, CloisNa exposure caused mitochondrial depolarization while Clois30B exposure caused reduced macrophage viability, greater cytotoxicity, and significant damage-associated molecular patterns (IL-1\u03b1 and ATP) release compared to CloisNa and unexposed controls. LPS priming with low CloisNa doses caused elevated cathepsin B\/Caspage-1\/IL-1\u03b2 release while higher doses resulted in apoptosis. Clois30B exposure caused dose-dependent THP-1 cell pyroptosis evidenced by Cathepsin B and IL-1\u03b2 release and Gasdermin D cleavage. Incineration ablated the cytotoxic and inflammatory effects of Clois30B while I-CloisNa still retained some mild inflammatory potential. Comparative analyses suggested that in vitro macrophage cell viability, inflammasome endpoints, and pro-inflammatory cytokine profiles significantly correlated to mouse bronchioalveolar lavage inflammation metrics including inflammatory cell recruitment.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Presence of organic coating and incineration status influenced inflammatory and cytotoxic responses following exposure to human macrophages. Clois30B, with a quaternary ammonium tallow coating, induced a robust cell membrane damage and pyroptosis effect which was eliminated after incineration. Conversely, incinerated nanoclay exposure primarily caused elevated inflammatory cytokine release from THP-1 cells. Collectively, pre-incinerated nanoclay displayed interaction with macrophage membrane components (molecular initiating event), increased pro-inflammatory mediators, and increased inflammatory cell recruitment (two key events) in the lung fibrosis adverse outcome pathway.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12989-024-00577-7","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T04:38:03Z","timestamp":1710995883000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["In vitro inflammation and toxicity assessment of pre- and post-incinerated organomodified nanoclays to macrophages using high-throughput screening approaches"],"prefix":"10.1186","volume":"21","author":[{"given":"Todd A.","family":"Stueckle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayme P.","family":"Coyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Derk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alixandra","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cerasela Zoica","family":"Dinu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany G.","family":"Kornberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherri A.","family":"Friend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Dozier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushant","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh K.","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying W.","family":"Rojanasakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"577_CR1","unstructured":"Research TM. Nanoclay (kaolinite, smectite, and others) market for packaging, flame retardants, automotive, paints & coatings, and other end-users- global industry analysis, size, share, growth, trends and forecast 2015\u22122023. 2015."},{"key":"577_CR2","doi-asserted-by":"crossref","unstructured":"Alateyah A, Dhakal H, Zhang ZJ. Processing, properties, and applications of polymer nanocomposites based on layer silicates: a review. Adv Polym Technol. 2013;32(4).","DOI":"10.1002\/adv.21368"},{"issue":"2","key":"577_CR3","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/BF02704606","volume":"29","author":"HA Patel","year":"2006","unstructured":"Patel HA, Somani RS, Bajaj HC, Jasra RV. Nanoclays for polymer nanocomposites, paints, inks, greases and cosmetics formulations, drug delivery vehicle and waste water treatment. Bull Mater Sci. 2006;29(2):133\u201345.","journal-title":"Bull Mater Sci"},{"issue":"1","key":"577_CR4","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.mrgentox.2012.10.006","volume":"751","author":"Y Huang","year":"2013","unstructured":"Huang Y, Zhang M, Zou H, Li X, Xing M, Fang X, He J. Genetic damage and lipid peroxidation in workers occupationally exposed to organic bentonite particles. Mutat Res Genet Toxicol Environ Mutagen. 2013;751(1):40\u20134.","journal-title":"Mutat Res Genet Toxicol Environ Mutagen"},{"key":"577_CR5","first-page":"177","volume":"16","author":"SLYZY Zeng","year":"1998","unstructured":"Zeng SLYZY, Zhang QF, Du YJ, Sun YT, Zhang MJ. Study on occupational standard of bentonite dust in air of workplace. Chin J Ind Hyg Occup Dis. 1998;16:177\u20138.","journal-title":"Chin J Ind Hyg Occup Dis"},{"issue":"6","key":"577_CR6","doi-asserted-by":"publisher","first-page":"641","DOI":"10.3109\/17435390.2015.1132346","volume":"10","author":"A Mackevica","year":"2016","unstructured":"Mackevica A, Foss HS. Release of nanomaterials from solid nanocomposites and consumer exposure assessment\u2014a forward-looking review. Nanotoxicology. 2016;10(6):641\u201353.","journal-title":"Nanotoxicology"},{"issue":"7","key":"577_CR7","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1007\/s11051-012-0989-z","volume":"14","author":"CS-J Tsai","year":"2012","unstructured":"Tsai CS-J, White D, Rodriguez H, Munoz CE, Huang C-Y, Tsai C-J, et al. Exposure assessment and engineering control strategies for airborne nanoparticles: an application to emissions from nanocomposite compounding processes. J Nanopart Res. 2012;14(7):989.","journal-title":"J Nanopart Res"},{"issue":"2","key":"577_CR8","doi-asserted-by":"publisher","first-page":"160","DOI":"10.4209\/aaqr.2008.01.0001","volume":"8","author":"S Tsai","year":"2008","unstructured":"Tsai S, Ashter A, Ada E, Mead JL, Barry CF, Ellenbecker MJJAAQR. Airborne nanoparticle release associated with the compounding of nanocomposites using nanoalumina as fillers. Aerosol Air Qual Res. 2008;8(2):160\u201377.","journal-title":"Aerosol Air Qual Res"},{"key":"577_CR9","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1186\/1743-8977-11-17","volume":"11","author":"SJ Froggett","year":"2014","unstructured":"Froggett SJ, Clancy SF, Boverhof DR, Canady RA. A review and perspective of existing research on the release of nanomaterials from solid nanocomposites. Part Fibre Toxicol. 2014;11:17.","journal-title":"Part Fibre Toxicol"},{"issue":"8","key":"577_CR10","doi-asserted-by":"publisher","first-page":"1951","DOI":"10.1039\/C8EN00420J","volume":"5","author":"J Pourchez","year":"2018","unstructured":"Pourchez J, Chivas-Joly C, Longuet C, Leclerc L, Sarry G, Lopez-Cuesta JM. End-of-life incineration of nanocomposites: new insights into nanofiller partitioning into by-products and biological outcomes of airborne emission and residual ash. Environ Sci Nano. 2018;5(8):1951\u201364.","journal-title":"Environ Sci Nano"},{"issue":"2","key":"577_CR11","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1180\/claymin.2009.044.2.161","volume":"44","author":"MC Floody","year":"2009","unstructured":"Floody MC, Theng B, Reyes P, Mora MJCM. Natural nanoclays: applications and future trends\u2013a Chilean perspective. Clay Miner. 2009;44(2):161\u201376.","journal-title":"Clay Miner"},{"issue":"3","key":"577_CR12","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.progsurf.2014.08.002","volume":"89","author":"AC Lopes","year":"2014","unstructured":"Lopes AC, Martins P, Lanceros-Mendez S. Aluminosilicate and aluminosilicate based polymer composites: present status, applications and future trends. Prog Surf Sci. 2014;89(3):239\u201377.","journal-title":"Prog Surf Sci"},{"key":"577_CR13","volume-title":"Bentonite, kaolin and selected clay minerals","author":"Z Adamis","year":"2005","unstructured":"Adamis Z, Williams RB. Bentonite, kaolin and selected clay minerals. 1st ed. Geneva: World Health Organization; 2005.","edition":"1"},{"issue":"1","key":"577_CR14","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1021\/acs.chemrestox.6b00409","volume":"30","author":"C Pavan","year":"2017","unstructured":"Pavan C, Fubini B. Unveiling the variability of \u201cquartz hazard\u201d in light of recent toxicological findings. Chem Res Toxicol. 2017;30(1):469\u201385.","journal-title":"Chem Res Toxicol"},{"key":"577_CR15","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.envres.2014.12.024","volume":"138","author":"S Maisanaba","year":"2015","unstructured":"Maisanaba S, Pichardo S, Puerto M, Gutierrez-Praena D, Camean AM, Jos A. Toxicological evaluation of clay minerals and derived nanocomposites: a review. Environ Res. 2015;138:233\u201354.","journal-title":"Environ Res"},{"issue":"1 Pt A","key":"577_CR16","doi-asserted-by":"publisher","first-page":"3406","DOI":"10.1016\/j.bbagen.2016.09.003","volume":"1861","author":"A Wagner","year":"2017","unstructured":"Wagner A, Eldawud R, White A, Agarwal S, Stueckle TA, Sierros KA, et al. Toxicity evaluations of nanoclays and thermally degraded byproducts through spectroscopical and microscopical approaches. Biochim Biophys Acta Gen Subj. 2017;1861(1 Pt A):3406\u201315.","journal-title":"Biochim Biophys Acta Gen Subj"},{"issue":"3","key":"577_CR17","doi-asserted-by":"publisher","first-page":"2292","DOI":"10.1021\/acsnano.7b07281","volume":"12","author":"TA Stueckle","year":"2018","unstructured":"Stueckle TA, Davidson DC, Derk R, Kornberg TG, Battelli L, Friend S, et al. Short-term pulmonary toxicity assessment of pre- and post-incinerated organomodified nanoclay in mice. ACS Nano. 2018;12(3):2292\u2013310.","journal-title":"ACS Nano"},{"issue":"12","key":"577_CR18","doi-asserted-by":"publisher","first-page":"2445","DOI":"10.1021\/acs.chemrestox.9b00277","volume":"32","author":"TA Stueckle","year":"2019","unstructured":"Stueckle TA, White A, Wagner A, Gupta RK, Rojanasakul Y, Dinu CZ. Impacts of organomodified nanoclays and their incinerated byproducts on bronchial cell monolayer integrity. Chem Res Toxicol. 2019;32(12):2445\u201358.","journal-title":"Chem Res Toxicol"},{"issue":"37","key":"577_CR19","doi-asserted-by":"publisher","first-page":"32323","DOI":"10.1021\/acsami.7b06657","volume":"9","author":"A Wagner","year":"2017","unstructured":"Wagner A, White AP, Stueckle TA, Banerjee D, Sierros KA, Rojanasakul Y, et al. Early assessment and correlations of nanoclay\u2019s toxicity to their physical and chemical properties. ACS Appl Mater Interfaces. 2017;9(37):32323\u201335.","journal-title":"ACS Appl Mater Interfaces"},{"issue":"1","key":"577_CR20","doi-asserted-by":"publisher","first-page":"10709","DOI":"10.1038\/s41598-018-28884-y","volume":"8","author":"A Wagner","year":"2018","unstructured":"Wagner A, White AP, Tang MC, Agarwal S, Stueckle TA, Rojanasakul Y, et al. Incineration of Nanoclay Composites Leads to Byproducts with Reduced Cellular Reactivity. Sci Rep. 2018;8(1):10709.","journal-title":"Sci Rep"},{"key":"577_CR21","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.jhazmat.2015.10.018","volume":"304","author":"S Maisanaba","year":"2016","unstructured":"Maisanaba S, Hercog K, Filipic M, Jos A, Zegura B. Genotoxic potential of montmorillonite clay mineral and alteration in the expression of genes involved in toxicity mechanisms in the human hepatoma cell line HepG2. J Hazard Mater. 2016;304:425\u201333.","journal-title":"J Hazard Mater"},{"issue":"3","key":"577_CR22","doi-asserted-by":"publisher","first-page":"279","DOI":"10.3109\/17435390.2013.776123","volume":"8","author":"G Janer","year":"2014","unstructured":"Janer G, Fernandez-Rosas E, Mas del Molino E, Gonzalez-Galvez D, Vilar G, Lopez-Iglesias C, et al. In vitro toxicity of functionalised nanoclays is mainly driven by the presence of organic modifiers. Nanotoxicology. 2014;8(3):279\u201394.","journal-title":"Nanotoxicology"},{"key":"577_CR23","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.mrgentox.2014.04.023","volume":"770","author":"AK Sharma","year":"2014","unstructured":"Sharma AK, Mortensen A, Schmidt B, Frandsen H, Hadrup N, Larsen EH, Binderup ML. In-vivo study of genotoxic and inflammatory effects of the organo-modified Montmorillonite Cloisite(R) 30B. Mutat Res Genet Toxicol Environ Mutagen. 2014;770:66\u201371.","journal-title":"Mutat Res Genet Toxicol Environ Mutagen"},{"issue":"1\u20133","key":"577_CR24","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S0300-483X(02)00061-6","volume":"175","author":"DW Porter","year":"2002","unstructured":"Porter DW, Barger M, Robinson VA, Leonard SS, Landsittel D, Castranova V. Comparison of low doses of aged and freshly fractured silica on pulmonary inflammation and damage in the rat. Toxicology. 2002;175(1\u20133):63\u201371.","journal-title":"Toxicology"},{"issue":"7","key":"577_CR25","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1080\/17435390.2020.1771786","volume":"14","author":"E Di Ianni","year":"2020","unstructured":"Di Ianni E, M\u00f8ller P, Mortensen A, Szarek J, Clausen PA, Saber AT, et al. Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs. Nanotoxicology. 2020;14(7):869\u201392.","journal-title":"Nanotoxicology"},{"key":"577_CR26","doi-asserted-by":"publisher","first-page":"503405","DOI":"10.1016\/j.mrgentox.2021.503405","volume":"872","author":"E Di Ianni","year":"2021","unstructured":"Di Ianni E, M\u00f8ller P, Vogel UB, Jacobsen NR. Pro-inflammatory response and genotoxicity caused by clay and graphene nanomaterials in A549 and THP-1 cells. Mutat Res Genet Toxicol Environ Mutagen. 2021;872:503405.","journal-title":"Mutat Res Genet Toxicol Environ Mutagen"},{"key":"577_CR27","doi-asserted-by":"publisher","first-page":"97","DOI":"10.3389\/fimmu.2016.00097","volume":"7","author":"KM Pollard","year":"2016","unstructured":"Pollard KM. Silica, silicosis, and autoimmunity. Front Immunol. 2016;7:97.","journal-title":"Front Immunol"},{"issue":"1","key":"577_CR28","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1186\/s12989-016-0162-4","volume":"13","author":"M Sayan","year":"2016","unstructured":"Sayan M, Mossman BT. The NLRP3 inflammasome in pathogenic particle and fibre-associated lung inflammation and diseases. Part Fibre Toxicol. 2016;13(1):51.","journal-title":"Part Fibre Toxicol"},{"issue":"2","key":"577_CR29","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1093\/toxsci\/kfu199","volume":"142","author":"DL Villeneuve","year":"2014","unstructured":"Villeneuve DL, Crump D, Garcia-Reyero N, Hecker M, Hutchinson TH, LaLone CA, et al. Adverse outcome pathway (AOP) development I: strategies and principles. Toxicol Sci. 2014;142(2):312\u201320.","journal-title":"Toxicol Sci"},{"issue":"5","key":"577_CR30","doi-asserted-by":"publisher","first-page":"232","DOI":"10.3390\/toxics10050232","volume":"10","author":"X Chang","year":"2022","unstructured":"Chang X, Tan YM, Allen DG, Bell S, Brown PC, Browning L, et al. IVIVE: Facilitating the use of in vitro toxicity data in risk assessment and decision making. Toxics. 2022;10(5):232.","journal-title":"Toxics"},{"issue":"1","key":"577_CR31","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s12989-023-00516-y","volume":"20","author":"P McLean","year":"2023","unstructured":"McLean P, Mueller W, Gosens I, Cassee FR, Rothen-Rutishauser B, Boyles M, Tran L. Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis. Part Fibre Toxicol. 2023;20(1):5.","journal-title":"Part Fibre Toxicol"},{"issue":"6","key":"577_CR32","doi-asserted-by":"publisher","first-page":"e1794","DOI":"10.1002\/wnan.1794","volume":"14","author":"E Di Ianni","year":"2022","unstructured":"Di Ianni E, Jacobsen NR, Vogel U, M\u00f8ller P. Predicting nanomaterials pulmonary toxicity in animals by cell culture models: Achievements and perspectives. Wiley Interdisc Rev Nanomed Nanobiotechnol. 2022;14(6):e1794.","journal-title":"Wiley Interdisc Rev Nanomed Nanobiotechnol"},{"key":"577_CR33","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.diff.2022.10.001","volume":"128","author":"ZN Mohd Yasin","year":"2022","unstructured":"Mohd Yasin ZN, Mohd Idrus FN, Hoe CH, Yvonne-Tee GB. Macrophage polarization in THP-1 cell line and primary monocytes: a systematic review. Differentiation. 2022;128:67\u201382.","journal-title":"Differentiation"},{"issue":"4","key":"577_CR34","doi-asserted-by":"publisher","first-page":"3207","DOI":"10.1002\/jcp.29268","volume":"235","author":"F Lu","year":"2020","unstructured":"Lu F, Lan Z, Xin Z, He C, Guo Z, Xia X, Hu T. Emerging insights into molecular mechanisms underlying pyroptosis and functions of inflammasomes in diseases. J Cell Physiol. 2020;235(4):3207\u201321.","journal-title":"J Cell Physiol"},{"issue":"3","key":"577_CR35","doi-asserted-by":"publisher","first-page":"57","DOI":"10.4103\/ed.ed_16_18","volume":"3","author":"Y Shang","year":"2018","unstructured":"Shang Y, Sun Q. Particulate air pollution: major research methods and applications in animal models. Environ Dis. 2018;3(3):57\u201362.","journal-title":"Environ Dis"},{"key":"577_CR36","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1007\/s10453-010-9179-6","volume":"27","author":"C Degobbi","year":"2011","unstructured":"Degobbi C, Saldiva PHN, Rogers C. Endotoxin as modifier of particulate matter toxicity: a review of the literature. Aerobiologia. 2011;27:97\u2013105.","journal-title":"Aerobiologia"},{"key":"577_CR37","unstructured":"Halappanavar S SM, Wallin H, Vogel U, Sullivan K, Clippinger AJ. AOP 173: Substance interaction with the lung resident cell membrane components leading to lung fibrosis 2023. https:\/\/aopwiki.org\/aops\/173."},{"key":"577_CR38","unstructured":"Castranova V, Dalal NS, Vallyathan V. Role of surface free radicals in the pathogenicity of silica. Silica and silicainduced diseases. Boca Raton: CRC Press. 1996. pp. 91\u2013105"},{"key":"577_CR39","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.impact.2017.12.002","volume":"10","author":"JM Cohen","year":"2018","unstructured":"Cohen JM, Beltran-Huarac J, Pyrgiotakis G, Demokritou P. Effective delivery of sonication energy to fast settling and agglomerating nanomaterial suspensions for cellular studies: Implications for stability, particle kinetics, dosimetry and toxicity. NanoImpact. 2018;10:81\u20136.","journal-title":"NanoImpact"},{"key":"577_CR40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12989-020-00371-1","volume":"17","author":"JPDR Coyle","year":"2020","unstructured":"Coyle JPDR, Kornberg TG, Singh D, Jensen J, Friend S, Mercer R, Stueckle TA, Demokritou P, Rojanasakul Y, Rojanasakul LW. Carbon nanotube filler enhances incinerated thermoplastics-induced cytotoxicity and metabolic disruption in vitro. Part Fibre Toxicol. 2020;17:1\u201321.","journal-title":"Part Fibre Toxicol"},{"key":"577_CR41","doi-asserted-by":"publisher","first-page":"3514","DOI":"10.1038\/ncomms4514","volume":"5","author":"G DeLoid","year":"2014","unstructured":"DeLoid G, Cohen JM, Darrah T, Derk R, Rojanasakul L, Pyrgiotakis G, et al. Estimating the effective density of engineered nanomaterials for in vitro dosimetry. Nat Commun. 2014;5:3514.","journal-title":"Nat Commun"},{"issue":"25","key":"577_CR42","doi-asserted-by":"publisher","first-page":"253103","DOI":"10.1063\/1.4937927","volume":"107","author":"Y Yue","year":"2015","unstructured":"Yue Y, Kan Y, Choi H, Clearfield A, Liang HJAPL. Correlating hydrodynamic radii with that of two-dimensional nanoparticles. Appl Phys Lett. 2015;107(25):253103.","journal-title":"Appl Phys Lett"},{"issue":"6","key":"577_CR43","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1289\/ehp.1306561","volume":"121","author":"T Xia","year":"2013","unstructured":"Xia T, Hamilton RF, Bonner JC, Crandall ED, Elder A, Fazlollahi F, et al. Interlaboratory evaluation of in vitro cytotoxicity and inflammatory responses to engineered nanomaterials: the NIEHS Nano GO Consortium. Environ Health Perspect. 2013;121(6):683\u201390.","journal-title":"Environ Health Perspect"},{"issue":"1","key":"577_CR44","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/s00011-007-6115-5","volume":"56","author":"EK Park","year":"2007","unstructured":"Park EK, Jung HS, Yang HI, Yoo MC, Kim C, Kim KS. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007;56(1):45\u201350.","journal-title":"Inflamm Res"},{"key":"577_CR45","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.impact.2016.11.001","volume":"6","author":"TA Stueckle","year":"2017","unstructured":"Stueckle TA, Davidson DC, Derk R, Kornberg TG, Schwegler-Berry D, Pirela SV, et al. Evaluation of tumorigenic potential of CeO2 and Fe2O3 engineered nanoparticles by a human cell in vitro screening model. NanoImpact. 2017;6:39\u201354.","journal-title":"NanoImpact"},{"issue":"11\u201312","key":"577_CR46","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1080\/15287390590912612","volume":"68","author":"A Hubbs","year":"2005","unstructured":"Hubbs A, Greskevitch M, Kuempel E, Suarez F, Toraason M. Abrasive blasting agents: designing studies to evaluate relative risk. J Toxicol Environ Health A. 2005;68(11\u201312):999\u20131016.","journal-title":"J Toxicol Environ Health A"},{"issue":"2","key":"577_CR47","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1164\/ajrccm\/146.2.454","volume":"146","author":"KC Stone","year":"1992","unstructured":"Stone KC, Mercer RR, Freeman BA, Chang LY, Crapo JD. Distribution of lung cell numbers and volumes between alveolar and nonalveolar tissue. Am Rev Respir Dis. 1992;146(2):454\u20136.","journal-title":"Am Rev Respir Dis"},{"key":"577_CR48","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/978-1-4939-7357-6_2","volume":"1683","author":"KM Chambers","year":"2018","unstructured":"Chambers KM, Mandavilli BS, Dolman NJ, Janes MS. General staining and segmentation procedures for high content imaging and analysis. Methods Mol Biol (Clifton, NJ). 2018;1683:21\u201331.","journal-title":"Methods Mol Biol (Clifton, NJ)"},{"key":"577_CR49","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/978-1-4939-7357-6_3","volume":"1683","author":"BS Mandavilli","year":"2018","unstructured":"Mandavilli BS, Aggeler RJ, Chambers KM. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis. Methods in molecular biology (Clifton, NJ). 2018;1683:33\u201346.","journal-title":"Methods in molecular biology (Clifton, NJ)"},{"issue":"3","key":"577_CR50","doi-asserted-by":"publisher","first-page":"1805","DOI":"10.1021\/nn102734s","volume":"5","author":"S George","year":"2011","unstructured":"George S, Xia T, Rallo R, Zhao Y, Ji Z, Lin S, et al. Use of a high-throughput screening approach coupled with in vivo zebrafish embryo screening to develop hazard ranking for engineered nanomaterials. ACS Nano. 2011;5(3):1805\u201317.","journal-title":"ACS Nano"},{"issue":"7610","key":"577_CR51","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1038\/nature18629","volume":"535","author":"X Liu","year":"2016","unstructured":"Liu X, Zhang Z, Ruan J, Pan Y, Magupalli VG, Wu H, Lieberman J. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores. Nature. 2016;535(7610):153\u20138.","journal-title":"Nature"},{"issue":"5","key":"577_CR52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v012.i05","volume":"12","author":"C Ritz","year":"2005","unstructured":"Ritz C, Streibig JCJJSS. Bioassay analysis using R. J Stat Softw. 2005;12(5):1\u201322.","journal-title":"J Stat Softw"},{"issue":"3","key":"577_CR53","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1021\/ar300022h","volume":"46","author":"A Nel","year":"2013","unstructured":"Nel A, Xia T, Meng H, Wang X, Lin S, Ji Z, Zhang H. Nanomaterial toxicity testing in the 21st century: use of a predictive toxicological approach and high-throughput screening. Acc Chem Res. 2013;46(3):607\u201321.","journal-title":"Acc Chem Res"},{"issue":"4","key":"577_CR54","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1002\/(SICI)1097-4636(20000315)49:4<469::AID-JBM5>3.0.CO;2-A","volume":"49","author":"AU Daniels","year":"2000","unstructured":"Daniels AU, Barnes FH, Charlebois SJ, Smith RA. Macrophage cytokine response to particles and lipopolysaccharide in vitro. J Biomed Mater Res. 2000;49(4):469\u201378.","journal-title":"J Biomed Mater Res"},{"key":"577_CR55","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/j.actbio.2020.03.017","volume":"108","author":"E J\u00e4msen","year":"2020","unstructured":"J\u00e4msen E, Pajarinen J, Kouri VP, Rahikkala A, Goodman SB, Manninen M, et al. Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages. Acta Biomater. 2020;108:347\u201357.","journal-title":"Acta Biomater"},{"issue":"2","key":"577_CR56","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1007\/s00204-005-0013-9","volume":"80","author":"S Geh","year":"2006","unstructured":"Geh S, Y\u00fccel R, Duffin R, Albrecht C, Borm PJ, Armbruster L, et al. Cellular uptake and cytotoxic potential of respirable bentonite particles with different quartz contents and chemical modifications in human lung fibroblasts. Arch Toxicol. 2006;80(2):98\u2013106.","journal-title":"Arch Toxicol"},{"issue":"1","key":"577_CR57","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1042\/CBI20100587","volume":"36","author":"S Lordan","year":"2012","unstructured":"Lordan S, Higginbotham CL. Effect of serum concentration on the cytotoxicity of clay particles. Cell Biol Int. 2012;36(1):57\u201361.","journal-title":"Cell Biol Int"},{"issue":"8","key":"577_CR58","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1038\/s41577-019-0165-0","volume":"19","author":"KV Swanson","year":"2019","unstructured":"Swanson KV, Deng M, Ting JP. The NLRP3 inflammasome: molecular activation and regulation to therapeutics. Nat Rev Immunol. 2019;19(8):477\u201389.","journal-title":"Nat Rev Immunol"},{"key":"577_CR59","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.taap.2017.01.012","volume":"318","author":"F Jessop","year":"2017","unstructured":"Jessop F, Hamilton RF Jr, Rhoderick JF, Fletcher P, Holian A. Phagolysosome acidification is required for silica and engineered nanoparticle-induced lysosome membrane permeabilization and resultant NLRP3 inflammasome activity. Toxicol Appl Pharmacol. 2017;318:58\u201368.","journal-title":"Toxicol Appl Pharmacol"},{"issue":"1","key":"577_CR60","doi-asserted-by":"publisher","first-page":"C83","DOI":"10.1152\/ajpcell.00298.2015","volume":"311","author":"MA Katsnelson","year":"2016","unstructured":"Katsnelson MA, Lozada-Soto KM, Russo HM, Miller BA, Dubyak GR. NLRP3 inflammasome signaling is activated by low-level lysosome disruption but inhibited by extensive lysosome disruption: roles for K+ efflux and Ca2+ influx. Am J Physiol Cell Physiol. 2016;311(1):C83-c100.","journal-title":"Am J Physiol Cell Physiol"},{"issue":"3","key":"577_CR61","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1007\/s10495-012-0798-y","volume":"18","author":"GN Joshi","year":"2013","unstructured":"Joshi GN, Knecht DA. Silica phagocytosis causes apoptosis and necrosis by different temporal and molecular pathways in alveolar macrophages. Apoptosis. 2013;18(3):271\u201385.","journal-title":"Apoptosis"},{"issue":"4","key":"577_CR62","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/s41419-023-05786-0","volume":"14","author":"Z Xie","year":"2023","unstructured":"Xie Z, Zhao M, Yan C, Kong W, Lan F, Narengaowa, et al. Cathepsin B in programmed cell death machinery: mechanisms of execution and regulatory pathways. Cell Death Disease. 2023;14(4):255.","journal-title":"Cell Death Disease"},{"issue":"10","key":"577_CR63","doi-asserted-by":"publisher","first-page":"4589","DOI":"10.7150\/thno.34065","volume":"10","author":"X Yuan","year":"2020","unstructured":"Yuan X, Nie W, He Z, Yang J, Shao B, Ma X, et al. Carbon black nanoparticles induce cell necrosis through lysosomal membrane permeabilization and cause subsequent inflammatory response. Theranostics. 2020;10(10):4589\u2013605.","journal-title":"Theranostics"},{"key":"577_CR64","doi-asserted-by":"publisher","first-page":"122997","DOI":"10.1016\/j.envpol.2023.122997","volume":"341","author":"L Caceres","year":"2024","unstructured":"Caceres L, Abogunloko T, Malchow S, Ehret F, Merz J, Li X, et al. Molecular mechanisms underlying NLRP3 inflammasome activation and IL-1\u03b2 production in air pollution fine particulate matter (PM(2.5))-primed macrophages. Environ Pollut (Barking, Essex: 1987). 2024;341:122997.","journal-title":"Environ Pollut (Barking, Essex: 1987)"},{"issue":"13","key":"577_CR65","doi-asserted-by":"publisher","first-page":"3328","DOI":"10.3390\/ijms20133328","volume":"20","author":"N Kelley","year":"2019","unstructured":"Kelley N, Jeltema D, Duan Y, He Y. The NLRP3 inflammasome: an overview of mechanisms of activation and regulation. Int J Mol Sci. 2019;20(13):3328.","journal-title":"Int J Mol Sci"},{"key":"577_CR66","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.molimm.2018.09.010","volume":"103","author":"Q Liu","year":"2018","unstructured":"Liu Q, Zhang D, Hu D, Zhou X, Zhou Y. The role of mitochondria in NLRP3 inflammasome activation. Mol Immunol. 2018;103:115\u201324.","journal-title":"Mol Immunol"},{"key":"577_CR67","doi-asserted-by":"publisher","first-page":"122950","DOI":"10.1016\/j.envpol.2023.122950","volume":"341","author":"LB Zhuo","year":"2024","unstructured":"Zhuo LB, Liu YM, Jiang Y, Yan Z. Zinc oxide nanoparticles induce acute lung injury via oxidative stress-mediated mitochondrial damage and NLRP3 inflammasome activation: In vitro and in vivo studies. Environ Pollut (Barking, Essex: 1987). 2024;341:122950.","journal-title":"Environ Pollut (Barking, Essex: 1987)"},{"issue":"5","key":"577_CR68","doi-asserted-by":"publisher","first-page":"1661","DOI":"10.1084\/jem.181.5.1661","volume":"181","author":"N Zamzami","year":"1991","unstructured":"Zamzami N, Marchetti P, Castedo M, Zanin C, Vayssiere J-L, Petit PX, Kroemer G. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J Exp Med. 1991;181(5):1661\u201372.","journal-title":"J Exp Med"},{"issue":"1","key":"577_CR69","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/s00204-019-02604-5","volume":"94","author":"S Deville","year":"2020","unstructured":"Deville S, Honrath B, Tran QTD, Fejer G, Lambrichts I, Nelissen I, et al. Time-resolved characterization of the mechanisms of toxicity induced by silica and amino-modified polystyrene on alveolar-like macrophages. Arch Toxicol. 2020;94(1):173\u201386.","journal-title":"Arch Toxicol"},{"issue":"2","key":"577_CR70","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.tiv.2011.12.006","volume":"26","author":"V Wilhelmi","year":"2012","unstructured":"Wilhelmi V, Fischer U, van Berlo D, Schulze-Osthoff K, Schins RP, Albrecht C. Evaluation of apoptosis induced by nanoparticles and fine particles in RAW 264.7 macrophages: facts and artefacts. Toxicol In Vitro. 2012;26(2):323\u201334.","journal-title":"Toxicol In Vitro"},{"key":"577_CR71","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1016\/j.fct.2013.03.043","volume":"57","author":"S Maisanaba","year":"2013","unstructured":"Maisanaba S, Puerto M, Pichardo S, Jorda M, Moreno FJ, Aucejo S, Jos A. In vitro toxicological assessment of clays for their use in food packaging applications. Food Chem Toxicol. 2013;57:266\u201375.","journal-title":"Food Chem Toxicol"},{"issue":"1\u20132","key":"577_CR72","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.mrgentox.2010.04.021","volume":"700","author":"AK Sharma","year":"2010","unstructured":"Sharma AK, Schmidt B, Frandsen H, Jacobsen NR, Larsen EH, Binderup ML. Genotoxicity of unmodified and organo-modified montmorillonite. Mutat Res. 2010;700(1\u20132):18\u201325.","journal-title":"Mutat Res"},{"issue":"4","key":"577_CR73","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v21.i4.10","volume":"21","author":"D Simberg","year":"2004","unstructured":"Simberg D, Weisman S, Talmon Y, Barenholz Y. DOTAP (and other cationic lipids): chemistry, biophysics, and transfection. Crit Rev Ther Drug Carrier Syst. 2004;21(4):257\u2013317.","journal-title":"Crit Rev Ther Drug Carrier Syst"},{"issue":"10","key":"577_CR74","doi-asserted-by":"publisher","first-page":"e102065","DOI":"10.15252\/embj.2019102065","volume":"38","author":"AM Gram","year":"2019","unstructured":"Gram AM, Booty LM, Bryant CE. Chopping GSDMD: caspase-8 has joined the team of pyroptosis-mediating caspases. EMBO J. 2019;38(10):e102065.","journal-title":"EMBO J"},{"key":"577_CR75","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/s12989-014-0069-x","volume":"11","author":"V Rabolli","year":"2014","unstructured":"Rabolli V, Badissi AA, Devosse R, Uwambayinema F, Yakoub Y, Palmai-Pallag M, et al. The alarmin IL-1\u03b1 is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles. Part Fibre Toxicol. 2014;11:69.","journal-title":"Part Fibre Toxicol"},{"issue":"1","key":"577_CR76","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/1743-8977-10-5","volume":"10","author":"JR Roberts","year":"2013","unstructured":"Roberts JR, Antonini JM, Porter DW, Chapman RS, Scabilloni JF, Young S-H, et al. Lung toxicity and biodistribution of Cd\/Se-ZnS quantum dots with different surface functional groups after pulmonary exposure in rats. Part Fibre Toxicol. 2013;10(1):5.","journal-title":"Part Fibre Toxicol"},{"issue":"2","key":"577_CR77","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/j.ccc.2010.12.005","volume":"27","author":"LJ Cross","year":"2011","unstructured":"Cross LJ, Matthay MA. Biomarkers in acute lung injury: insights into the pathogenesis of acute lung injury. Crit Care Clin. 2011;27(2):355\u201377.","journal-title":"Crit Care Clin"},{"issue":"1","key":"577_CR78","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1111\/imr.12577","volume":"280","author":"D Yang","year":"2017","unstructured":"Yang D, Han Z, Oppenheim JJ. Alarmins and immunity. Immunol Rev. 2017;280(1):41\u201356.","journal-title":"Immunol Rev"},{"issue":"3","key":"577_CR79","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/ni.2215","volume":"13","author":"CS Shi","year":"2012","unstructured":"Shi CS, Shenderov K, Huang NN, Kabat J, Abu-Asab M, Fitzgerald KA, et al. Activation of autophagy by inflammatory signals limits IL-1\u03b2 production by targeting ubiquitinated inflammasomes for destruction. Nat Immunol. 2012;13(3):255\u201363.","journal-title":"Nat Immunol"},{"issue":"4","key":"577_CR80","first-page":"261","volume":"4","author":"T Otsuki","year":"2007","unstructured":"Otsuki T, Maeda M, Murakami S, Hayashi H, Miura Y, Kusaka M, et al. Immunological effects of silica and asbestos. Cell Mol Immunol. 2007;4(4):261\u20138.","journal-title":"Cell Mol Immunol"},{"issue":"11","key":"577_CR81","doi-asserted-by":"publisher","first-page":"6367","DOI":"10.4049\/jimmunol.0900459","volume":"184","author":"SL Re","year":"2010","unstructured":"Re SL, Dumoutier L, Couillin I, Van Vyve C, Yakoub Y, Uwambayinema F, et al. IL-17A\u2013Producing \u03b3\u03b4 T and Th17 Lymphocytes Mediate Lung Inflammation but Not Fibrosis in Experimental Silicosis. J Immunol. 2010;184(11):6367\u201377.","journal-title":"J Immunol"},{"issue":"1","key":"577_CR82","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.taap.2013.11.012","volume":"275","author":"Y Chen","year":"2014","unstructured":"Chen Y, Li C, Weng D, Song L, Tang W, Dai W, et al. Neutralization of interleukin-17A delays progression of silica-induced lung inflammation and fibrosis in C57BL\/6 mice. Toxicol Appl Pharmacol. 2014;275(1):62\u201372.","journal-title":"Toxicol Appl Pharmacol"},{"issue":"1","key":"577_CR83","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s12989-020-00345-3","volume":"17","author":"T Skuland","year":"2020","unstructured":"Skuland T, L\u00e5g M, Gutleb AC, Brinchmann BC, Serchi T, \u00d8vrevik J, et al. Pro-inflammatory effects of crystalline- and nano-sized non-crystalline silica particles in a 3D alveolar model. Part Fibre Toxicol. 2020;17(1):13.","journal-title":"Part Fibre Toxicol"},{"issue":"2","key":"577_CR84","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1080\/17435390.2017.1287313","volume":"11","author":"D Breznan","year":"2017","unstructured":"Breznan D, Das DD, O\u2019Brien JS, MacKinnon-Roy C, Nimesh S, Vuong NQ, et al. Differential cytotoxic and inflammatory potency of amorphous silicon dioxide nanoparticles of similar size in multiple cell lines. Nanotoxicology. 2017;11(2):223\u201335.","journal-title":"Nanotoxicology"},{"key":"577_CR85","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.3389\/fimmu.2018.01120","volume":"9","author":"J Dong","year":"2018","unstructured":"Dong J, Ma Q. Type 2 immune mechanisms in carbon nanotube-induced lung fibrosis. Front Immunol. 2018;9:1120.","journal-title":"Front Immunol"},{"key":"577_CR86","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1146\/annurev-immunol-032712-095937","volume":"31","author":"G Wick","year":"2013","unstructured":"Wick G, Grundtman C, Mayerl C, Wimpissinger T-F, Feichtinger J, Zelger B, et al. The immunology of fibrosis. Annu Rev Immunol. 2013;31:107\u201335.","journal-title":"Annu Rev Immunol"},{"key":"577_CR87","doi-asserted-by":"crossref","unstructured":"Barbarin V, Xing Z, Delos M, Lison D, Huaux FJAJoP-LC, Physiology M. Pulmonary overexpression of IL-10 augments lung fibrosis and Th2 responses induced by silica particles. Am J Physiol Lung Cell Mol Physiol 2005;288(5):L841-L8.","DOI":"10.1152\/ajplung.00329.2004"},{"key":"577_CR88","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/1743-8977-10-26","volume":"10","author":"C Brandenberger","year":"2013","unstructured":"Brandenberger C, Rowley NL, Jackson-Humbles DN, Zhang Q, Bramble LA, Lewandowski RP, et al. Engineered silica nanoparticles act as adjuvants to enhance allergic airway disease in mice. Part Fibre Toxicol. 2013;10:26.","journal-title":"Part Fibre Toxicol"},{"issue":"4","key":"577_CR89","doi-asserted-by":"publisher","first-page":"647","DOI":"10.2478\/s13382-013-0137-8","volume":"26","author":"R Swiercz","year":"2013","unstructured":"Swiercz R, Ha\u0142atek T, Stetkiewicz J, W\u0105sowicz W, Kur B, Grzeli\u0144ska Z, Majcherek W. Toxic effect in the lungs of rats after inhalation exposure to benzalkonium chloride. Int J Occup Med Environ Health. 2013;26(4):647\u201356.","journal-title":"Int J Occup Med Environ Health"},{"issue":"2 Pt 1","key":"577_CR90","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1053\/ai.1994.v94.a52646","volume":"94","author":"JA Bernstein","year":"1994","unstructured":"Bernstein JA, Stauder T, Bernstein DI, Bernstein IL. A combined respiratory and cutaneous hypersensitivity syndrome induced by work exposure to quaternary amines. J Allergy Clin Immunol. 1994;94(2 Pt 1):257\u20139.","journal-title":"J Allergy Clin Immunol"},{"issue":"1","key":"577_CR91","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/rr177","volume":"3","author":"MP Keane","year":"2002","unstructured":"Keane MP, Strieter RM. The importance of balanced pro-inflammatory and anti-inflammatory mechanisms in diffuse lung disease. Respir Res. 2002;3(1):5.","journal-title":"Respir Res"},{"issue":"7","key":"577_CR92","doi-asserted-by":"publisher","first-page":"3755","DOI":"10.4049\/jimmunol.1302470","volume":"193","author":"TT Le","year":"2014","unstructured":"Le TT, Karmouty-Quintana H, Melicoff E, Le TT, Weng T, Chen NY, et al. Blockade of IL-6 Trans signaling attenuates pulmonary fibrosis. J Immunol (Baltimore, Md: 1950). 2014;193(7):3755\u201368.","journal-title":"J Immunol (Baltimore, Md: 1950)"},{"issue":"2","key":"577_CR93","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1089\/107999003321455453","volume":"23","author":"SM Fitzgerald","year":"2003","unstructured":"Fitzgerald SM, Chi DS, Hall HK, Reynolds SA, Aramide O, Lee SA, et al. GM-CSF induction in human lung fibroblasts by IL-1 \u03b2, TNF-\u03b1, and macrophage contact. J Interferon Cytokine Res. 2003;23(2):57\u201365.","journal-title":"J Interferon Cytokine Res"},{"issue":"1\u20132","key":"577_CR94","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/S0300-483X(00)00415-7","volume":"159","author":"DS Lang","year":"2001","unstructured":"Lang DS, Schocker H, Hockertz S. Effects of crocidolite asbestos on human bronchoepithelial-dependent fibroblast stimulation in coculture: the role of IL-6 and GM-CSF. Toxicology. 2001;159(1\u20132):81\u201398.","journal-title":"Toxicology"},{"issue":"7","key":"577_CR95","doi-asserted-by":"publisher","first-page":"4618","DOI":"10.4049\/jimmunol.172.7.4618","volume":"172","author":"F Mor","year":"2004","unstructured":"Mor F, Quintana FJ, Cohen IR. Angiogenesis-inflammation cross-talk: vascular endothelial growth factor is secreted by activated T cells and induces Th1 polarization. J Immunol (Baltimore, Md: 1950). 2004;172(7):4618\u201323.","journal-title":"J Immunol (Baltimore, Md: 1950)"},{"issue":"13","key":"577_CR96","doi-asserted-by":"publisher","first-page":"5360","DOI":"10.1073\/pnas.1015476108","volume":"108","author":"M Fei","year":"2011","unstructured":"Fei M, Bhatia S, Oriss TB, Yarlagadda M, Khare A, Akira S, et al. TNF-\u03b1 from inflammatory dendritic cells (DCs) regulates lung IL-17A\/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection. Proc Natl Acad Sci USA. 2011;108(13):5360\u20135.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"2","key":"577_CR97","first-page":"135","volume":"4","author":"R Ullah","year":"2014","unstructured":"Ullah R, Deb BK, Mollah MYA. Synthesis and characterization of silica coated iron-oxide composites of different ratios. Int J Compos Mater. 2014;4(2):135\u201345.","journal-title":"Int J Compos Mater"},{"issue":"5","key":"577_CR98","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1590\/S1516-14392013005000114","volume":"16","author":"L Bieseki","year":"2013","unstructured":"Bieseki L, Bertell F, Treichel H, Penha FG, Pergher SBC. Acid treatments of montmorillonite-rich clay for Fe removal using a factorial design method. Mater Res. 2013;16(5):1122\u20137.","journal-title":"Mater Res"},{"issue":"1","key":"577_CR99","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1515\/msp-2015-0008","volume":"33","author":"M Munasir","year":"2015","unstructured":"Munasir M, Triwikantoro T, Zainuri M, Darminto D. Synthesis of SiO2 nanopowders containing quartz and cristobalite phases from silica sands. Mater Sci Pol. 2015;33(1):47\u201355.","journal-title":"Mater Sci Pol"},{"issue":"4","key":"577_CR100","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1080\/08958370252870998","volume":"14","author":"DW Porter","year":"2002","unstructured":"Porter DW, Ye J, Ma J, Barger M, Robinson VA, Ramsey D, et al. Time course of pulmonary response of rats to inhalation of crystalline silica: NF-kappa B activation, inflammation, cytokine production, and damage. Inhalation Toxicol. 2002;14(4):349\u201367.","journal-title":"Inhalation Toxicol"},{"issue":"1","key":"577_CR101","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/1743-8977-7-39","volume":"7","author":"D Napierska","year":"2010","unstructured":"Napierska D, Thomassen LC, Lison D, Martens JA, Hoet PH. The nanosilica hazard: another variable entity. Part Fibre Toxicol. 2010;7(1):39.","journal-title":"Part Fibre Toxicol"},{"issue":"26","key":"577_CR102","first-page":"6833","volume":"31","author":"T Morishige","year":"2010","unstructured":"Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Narimatsu S, et al. The effect of surface modification of amorphous silica particles on NLRP3 inflammasome mediated IL-1\u03b2 production. ROS Prod Endosomal Rupture Biomater. 2010;31(26):6833\u201342.","journal-title":"ROS Prod Endosomal Rupture Biomater"},{"issue":"1","key":"577_CR103","doi-asserted-by":"publisher","first-page":"e1413","DOI":"10.1002\/wnan.1413","volume":"9","author":"AR Collins","year":"2017","unstructured":"Collins AR, Annangi B, Rubio L, Marcos R, Dorn M, Merker C, et al. High throughput toxicity screening and intracellular detection of nanomaterials. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017;9(1):e1413.","journal-title":"Wiley Interdiscip Rev Nanomed Nanobiotechnol"},{"issue":"5","key":"577_CR104","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/s11051-017-3850-6","volume":"19","author":"R Landsiedel","year":"2017","unstructured":"Landsiedel R, Ma-Hock L, Wiench K, Wohlleben W, Sauer UG. Safety assessment of nanomaterials using an advanced decision-making framework, the DF4nanoGrouping. J Nanopart Res. 2017;19(5):171.","journal-title":"J Nanopart Res"},{"key":"577_CR105","unstructured":"CRC Handbook of Chemistry and Physics. 92nd Edition. Anticancer Research. 2012;32(5):2220-."}],"container-title":["Particle and Fibre Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12989-024-00577-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12989-024-00577-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12989-024-00577-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T05:02:25Z","timestamp":1710997345000},"score":1,"resource":{"primary":{"URL":"https:\/\/particleandfibretoxicology.biomedcentral.com\/articles\/10.1186\/s12989-024-00577-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,21]]},"references-count":105,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["577"],"URL":"https:\/\/doi.org\/10.1186\/s12989-024-00577-7","relation":{},"ISSN":["1743-8977"],"issn-type":[{"value":"1743-8977","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,21]]},"assertion":[{"value":"14 July 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 March 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal study protocol and procedures were approved by the NIOSH IACUC.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests. The findings and conclusions in this article are those of the author(s) and do not necessarily represent the official position of the National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention. Mention of product names does not constitute endorsement.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"16"}}